Vibrio Anguillarum

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Debra L. Milton - One of the best experts on this subject based on the ideXlab platform.

Barbara Weber - One of the best experts on this subject based on the ideXlab platform.

Chang Chen - One of the best experts on this subject based on the ideXlab platform.

Jorge H. Crosa - One of the best experts on this subject based on the ideXlab platform.

  • Genetic Determinants of Virulence in the Marine Fish Pathogen Vibrio Anguillarum
    Fish pathology, 2011
    Co-Authors: Hiroaki Naka, Jorge H. Crosa
    Abstract:

    One of the most studied fish pathogens is Vibrio Anguillarum. Development of the genetics and biochemistry of the mechanisms of virulence in this fish pathogen together with clinical and ecologic studies has permitted the intensive development of microbiology in fish diseases. It is the intention of this review to compile the exhaustive knowledge accumulated on this bacterium and its interaction with the host fish by reporting a complete analysis of the V. Anguillarum virulence factors and the genetics of their complexity.

  • Characterization of ferric-anguibactin transport in Vibrio Anguillarum
    BioMetals, 2007
    Co-Authors: Claudia S. López, Jorge H. Crosa
    Abstract:

    The fish pathogen Vibrio Anguillarum is the causative agent of a fatal hemorrhagic septicemia in salmonid fish. Many serotype O1 strains harbors a 65 Kbp plasmid (pJM1 encoding an iron sequestering system essential for virulence. The genes involved in the biosynthesis of the indigenous siderophore anguibactin are encoded by both the pJM1 plasmid and the chromosome, while those involved in the transport of the ferric-siderophore complex, including the outer membrane receptor, are plasmid-encoded. This work describes the role of specific amino acid residues of the outer membrane receptor FatA in the mechanism of transport of ferric-anguibactin. FatA modeling indicated that this protein has a 22 stranded ß-barrel blocked by the plug domain, the latter being formed by residues 51–54. Deletion of the plug domain resulted in a receptor unable to act as an open channel for the transport of the ferric anguibactin complex.

  • Characterization of ferric-anguibactin transport in Vibrio Anguillarum.
    Biometals : an international journal on the role of metal ions in biology biochemistry and medicine, 2007
    Co-Authors: Claudia S. López, Jorge H. Crosa
    Abstract:

    The fish pathogen Vibrio Anguillarum is the causative agent of a fatal hemorrhagic septicemia in salmonid fish. Many serotype O1 strains harbors a 65 Kbp plasmid (pJM1 encoding an iron sequestering system essential for virulence. The genes involved in the biosynthesis of the indigenous siderophore anguibactin are encoded by both the pJM1 plasmid and the chromosome, while those involved in the transport of the ferric-siderophore complex, including the outer membrane receptor, are plasmid-encoded. This work describes the role of specific amino acid residues of the outer membrane receptor FatA in the mechanism of transport of ferric-anguibactin. FatA modeling indicated that this protein has a 22 stranded beta-barrel blocked by the plug domain, the latter being formed by residues 51-154. Deletion of the plug domain resulted in a receptor unable to act as an open channel for the transport of the ferric anguibactin complex.

  • Multiplex PCR for simultaneous detection of five virulence hemolysin genes in Vibrio Anguillarum.
    Journal of microbiological methods, 2005
    Co-Authors: Channarong Rodkhum, Jorge H. Crosa, Ikuo Hirono, Takashi Aoki
    Abstract:

    A multiplex PCR was developed for detection of hemolysin-producing Vibrio Anguillarum using primers targeting five hemolysin genes (vah1, vah2, vah3, vah4 and vah5). This method was successful in amplifying reactions containing as little as 100 fg of genomic template DNA. The direct detection of V. Anguillarum in clinical specimens by this multiplex PCR was also successful in reactions containing as few as 10 bacterial cells. This multiplex PCR method can be a rapid and sensitive method for detecting pathogenic V. Anguillarum.

  • Two tonB Systems Function in Iron Transport in Vibrio Anguillarum, but Only One Is Essential for Virulence
    Infection and immunity, 2004
    Co-Authors: Michiel Stork, Manuel L. Lemos, Manuela Di Lorenzo, Susana Mouriño, Carlos R. Osorio, Jorge H. Crosa
    Abstract:

    We have identified two functional tonB systems in the marine fish pathogen Vibrio Anguillarum, tonB1 and tonB2. Each of the tonB genes is transcribed in an operon with the cognate exbB and exbD genes in response to iron limitation. Only tonB2 is essential for transport of ferric anguibactin and virulence.

Yuanxing Zhang - One of the best experts on this subject based on the ideXlab platform.

  • transcriptome profiling reveals th17 like immune responses induced in zebrafish bath vaccinated with a live attenuated Vibrio Anguillarum
    PLOS ONE, 2013
    Co-Authors: Hua Zhang, Qin Liu, Qiyao Wang, Chao Fei, Minjun Yang, Yuanxing Zhang
    Abstract:

    Background A candidate vaccine, live attenuated Vibrio Anguillarum developed in our laboratory could prevent Vibriosis of fish resulted from V. Anguillarum and V. alginolyticus. To elucidate the molecular mechanisms underlying the vaccine protection, we used microarray technology to compare the spleen transcriptomes of bath-vaccinated and unvaccinated zebrafish at 28 days post vaccination.

  • FERMENTATION PREPARATION OF RECOMBINANT Vibrio Anguillarum VACCINE WITH HETEROGENEOUS ANTIGEN DISPLAY
    Preparative biochemistry & biotechnology, 2013
    Co-Authors: Sanying Wang, Qin Liu, Menghao Cai, Qiyao Wang, Yuanxing Zhang
    Abstract:

    In the design of recombinant bacterial vector vaccine, heterogeneous antigen is displayed on the outer membrane of the vector strain to evoke polyvalent immunological protection. Thus, the expression of heterogeneous antigen in cells and its display on the outer membrane are of great concern for vaccine preparation. In our previous work, a multivalent bacterial vector vaccine MVAV6203A-1 was constructed by displaying the protective antigen GAPDH from Aeromonas hydrophila on the surface of an attenuated Vibrio Anguillarum MVAV6203. In this work, a new fermentation medium was designed by a four-step method to improve the cell growth and antigen display of V. Anguillarum MVAV6203A-1. First, suitable carbon and nitrogen sources were selected by a component swapping method. Second, the initial concentrations of carbon and nitrogen sources were determined by orthogonal design. Then three main factors to significantly affect cell growth and antigen expression were screened by a Plackett–Burman design. Finally, t...

  • Different approaches to expressing Edwardsiella tarda antigen GAPDH in attenuated Vibrio Anguillarum for multivalent fish vaccines
    Journal of fish diseases, 2012
    Co-Authors: Y Zheng, Yuanxing Zhang, Qiyao Wang, Yichuan Xiao, Jingfan Xiao, Qinghai Liu
    Abstract:

    With the development of gene technology, expressing heterologous antigens in attenuated bacteria has become an important strategy to design multivalent vaccines. In our previous work, an attenuated Vibrio Anguillarum named MVAV6203 was developed and proven to be an efficient live vaccine candidate. In this research, we aimed to express protective antigen glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Edwardsiella tarda in attenuated Vibrio Anguillarum to establish a multivalent V. Anguillarum vector vaccine. Several strategies were compared between low- vs. high-copy plasmid-mediated antigen expression, in vivo-inducible vs. constitutive antigen expression and intracellular vs. surface-displaying antigen expression. Zebrafish, Danio rerio (Hamilton), was applied as the fish model to evaluate the immune protection of the V. Anguillarum vector vaccine candidates. Our results demonstrated that V. Anguillarum MVAV6203 (pUTatLNG40), which harbours a low-copy plasmid-loaded antigen surface display system under the control of a constitutive promoter, presented the best protective efficacy against the infection of Vibrio Anguillarum (relative per cent survival, RPS = 85%) and Edwardsiella tarda (RPS = 70%).

  • Freeze-drying of live attenuated Vibrio Anguillarum mutant for vaccine preparation.
    Biologicals : journal of the International Association of Biological Standardization, 2007
    Co-Authors: Lu Yang, Yuanxing Zhang
    Abstract:

    Vibrio Anguillarum MVAV6203 is a mutant strain as a candidate of live attenuated vaccine. In vaccine preparation, the freeze-drying conditions of the strain were investigated to improve the survival after freeze-drying, including the protectant, rehydration medium, freezing temperature, and initial cell concentration. Vibrio Anguillarum MVAV6203 is sensitive to freeze-drying and the viability was only 0.03% in the absence of protectant. Of the tested protectants, 5% trehalose with 15% skimmed milk gave the highest viability of 34.2%. Higher cell survival was obtained by quick freezing at -80 degrees C than slow freezing at -20 degrees C. Initial cell concentration was another important factor, preferable for 1-3 x 10(10)CFU/ml. The supplementation of 10% skimmed milk in rehydration medium improved obviously freeze-drying viability. The combination of the optimal conditions achieved 51.4% cell viability after freeze-drying.

  • DNA sequencing of a plasmid with virulence from marine fish pathogen Vibrio Anguillarum.
    Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 2003
    Co-Authors: Huizhan Zhang, Na Liang, Hui-yi Jin, Yuanxing Zhang
    Abstract:

    DNA sequence of a plasmid pEIB1 associated with virulence from the marine fish pathogen Vibrio Anguillarum was determined using the methods of restriction endonuclease digestion, subcloning, and primer walking. The whole length of obtained pEIB1 DNA sequence was 66 164 bp, and the overall G+C content of DNA sequence is 42.7%. This sequence encodes 44 open reading frames containing the genes of DNA replication, biosynthesis and regulation of the siderophore anguibactin and transport of ferric-anguibactin complexes.

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