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Tom Defoirdt - One of the best experts on this subject based on the ideXlab platform.
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Quorum sensing-disrupting compounds protect larvae of the giant freshwater prawn Macrobrachium rosenbergii from Vibrio harveyi infection
Aquaculture, 2013Co-Authors: Gde Sasmita Julyantoro Pande, Mathieu Wille, Patrick Sorgeloos, Peter Bossier, Anne Aamdal Scheie, Tore Benneche, Tom DefoirdtAbstract:Abstract Vibriosis outbreaks caused by Vibrio harveyi and related species are amongst the major obstacles for the further expansion of giant freshwater prawn ( Macrobrachium rosenbergii ) larviculture. Vibrio harveyi regulates virulence gene expression through quorum sensing, bacterial cell-to-cell communication, and consequently, quorum sensing disruption has been suggested as an alternative strategy to control infections caused by these bacteria. Previous studies have shown that quorum sensing-disrupting compounds are able to disrupt quorum sensing in Vibrio harveyi . In this study, we demonstrated that the quorum sensing-disrupting compounds cinnamaldehyde, ( Z -)-4-bromo-5-(bromomethylene)-2(5 H )-furanone and ( Z )-4-((5-(bromomethylene)-2-oxo-2,5-dihydrothiophen-3-yl)metoxy)-4-oxobutanoic acid increased the survival of giant freshwater prawn larvae when challenged to pathogenic Vibrio harveyi . Our in vivo challenge test showed that cinnamaldehyde and the thiophenone can protect the larvae from Vibrio harveyi infection when dosed to the culture water at 1 μM and 10 μM, whereas the brominated furanone offered protection at 1 μM but resulted in complete mortality at 10 μM. Although there were significant differences in survival between challenged larvae with and without addition of quorum sensing-disrupting compounds, there were no differences in growth (as determined by the larval stage index).
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Monitoring of Vibrio harveyi quorum sensing activity in real time during infection of brine shrimp larvae.
The ISME journal, 2012Co-Authors: Tom Defoirdt, Patrick SorgeloosAbstract:Quorum sensing, bacterial cell-to-cell communication, has been linked to the virulence of pathogenic bacteria. Indeed, in vitro experiments have shown that many bacterial pathogens regulate the expression of virulence genes by this cell-to-cell communication process. Moreover, signal molecules have been detected in samples retrieved from infected hosts and quorum sensing disruption has been reported to result in reduced virulence in different host–pathogen systems. However, data on in vivo quorum sensing activity of pathogens during infection of a host are currently lacking. We previously reported that quorum sensing regulates the virulence of Vibrio harveyi in a standardised model system with gnotobiotic brine shrimp (Artemia franciscana) larvae. Here, we monitored quorum sensing activity in Vibrio harveyi during infection of the shrimp, using bioluminescence as a read-out. We found that wild-type Vibrio harveyi shows a strong increase in quorum sensing activity early during infection. In this respect, the bacteria behave remarkably similar in different larvae, despite the fact that only half of them survive the infection. Interestingly, when expressed per bacterial cell, Vibrio harveyi showed around 200-fold higher maximal quorum sensing-regulated bioluminescence when associated with larvae than in the culture water. Finally, the in vivo quorum sensing activity of mutants defective in the production of one of the three signal molecules is consistent with their virulence, with no detectable in vivo quorum sensing activity in AI-2- and CAI-1-deficient mutants. These results indicate that AI-2 and CAI-1 are the dominant signals during infection of brine shrimp.
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Quorum quenching bacteria protect Macrobrachium rosenbergii larvae from Vibrio harveyi infection.
Journal of Applied Microbiology, 2010Co-Authors: Do Thi Thanh Nhan, Tom Defoirdt, Mathieu Wille, Peter Bossier, Patrick SorgeloosAbstract:Aims: In this study, we investigated the effect of N-acyl homoserine lactone-degrading bacterial enrichment cultures (ECs) on larviculture of the giant freshwater prawn Macrobrachium rosenbergii. Methods and Results: The larval performance in terms of larval growth, larval survival, larval quality, duration of the larval rearing process and microflora levels in the rearing water as well as inside the prawn gut was investigated. The application of the EC bacteria was performed in two ways: by adding them directly into the larval rearing water and via enriched Artemia nauplii used for larval feeding. The results of the study demonstrated that both ECs that were tested had a similar positive effect on larval survival and larval quality, whereas they did not affect larval growth or the duration of the larval rearing process. Conclusions: Under normal hatchery conditions, the optimal EC densities were found to be 106 CFU ml-1 for adding into the rearing water and 5 × 108 CFU ml-1 for enrichment of Artemia nauplii used for feeding of the larvae. In the hatchery, the ECs can be grown on waste streams of Artemia hatching. Significance and Impact of the Study: Application of this kind of ECs could lead to a more sustainable aquaculture production, by replacing the use of antibiotics to control diseases. © 2010 The Society for Applied Microbiology. Author Keywords: bacteria; enrichment cultures; Macrobrachium rosenbergii; Vibrio harveyi Index Keywords: ammonia; n acylhomoserine lactone; bacterial disease; bacterium; biodegradation; biological control; biotechnology; disease control; freshwater environment; growth rate; larval development; larviculture; prawn culture; survival; sustainable development; animal cell; animal experiment; animal model; article; bacterium culture; controlled study; dose response; enrichment culture; enteric feeding; hatchery; intestine; larva; larval development; larval stage; macrobrachium rosenbergii; metamorphosis; microflora; nonhuman; quorum sensing; shrimp; survival; toxicity testing; Vibrio harveyi; Vibriosis; Artemia; Bacteria (microorganisms); Macrobrachium rosenbergii; Vibrio harveyi
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Luminescence, virulence and quorum sensing signal production by pathogenic Vibrio campbellii and Vibrio harveyi isolates.
Journal of applied microbiology, 2007Co-Authors: Tom Defoirdt, Willy Verstraete, Peter BossierAbstract:Aims: To study the relationship between luminescence, autoinducer production and virulence of pathogenic Vibrios. Methods and Results: Luminescence, quorum sensing signal production and virulence towards brine shrimp nauplii of 13 Vibrio campbellii and Vibrio harveyi strains were studied. Although only two of the tested strains were brightly luminescent, all of them were shown to produce the three different types of quorum sensing signals known to be produced by Vibrio harveyi. Cell-free culture fluids of all strains significantly induced bioluminescence in the cholerae autoinducer 1, autoinducer 2 and harveyi autoinducer 1 reporter strains JAF375, JMH597 and JMH612, respectively. There was no relation between luminescence and signal production and virulence towards brine shrimp. Conclusions: There is a large difference between different strains of Vibrio campbellii and Vibrio harveyi with respect to bioluminescence. However, this is not reflected in signal production and virulence towards gnotobiotic brine shrimp. Moreover, there seems to be no relation between quorum sensing signal production and virulence towards brine shrimp. Significance and Impact of the Study: The results presented here indicate that strains that are most brightly luminescent are not necessarily the most virulent ones and that the lower virulence of some of the strains is not due to a lack of autoinducer production.
Patrick Sorgeloos - One of the best experts on this subject based on the ideXlab platform.
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Quorum sensing-disrupting compounds protect larvae of the giant freshwater prawn Macrobrachium rosenbergii from Vibrio harveyi infection
Aquaculture, 2013Co-Authors: Gde Sasmita Julyantoro Pande, Mathieu Wille, Patrick Sorgeloos, Peter Bossier, Anne Aamdal Scheie, Tore Benneche, Tom DefoirdtAbstract:Abstract Vibriosis outbreaks caused by Vibrio harveyi and related species are amongst the major obstacles for the further expansion of giant freshwater prawn ( Macrobrachium rosenbergii ) larviculture. Vibrio harveyi regulates virulence gene expression through quorum sensing, bacterial cell-to-cell communication, and consequently, quorum sensing disruption has been suggested as an alternative strategy to control infections caused by these bacteria. Previous studies have shown that quorum sensing-disrupting compounds are able to disrupt quorum sensing in Vibrio harveyi . In this study, we demonstrated that the quorum sensing-disrupting compounds cinnamaldehyde, ( Z -)-4-bromo-5-(bromomethylene)-2(5 H )-furanone and ( Z )-4-((5-(bromomethylene)-2-oxo-2,5-dihydrothiophen-3-yl)metoxy)-4-oxobutanoic acid increased the survival of giant freshwater prawn larvae when challenged to pathogenic Vibrio harveyi . Our in vivo challenge test showed that cinnamaldehyde and the thiophenone can protect the larvae from Vibrio harveyi infection when dosed to the culture water at 1 μM and 10 μM, whereas the brominated furanone offered protection at 1 μM but resulted in complete mortality at 10 μM. Although there were significant differences in survival between challenged larvae with and without addition of quorum sensing-disrupting compounds, there were no differences in growth (as determined by the larval stage index).
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Monitoring of Vibrio harveyi quorum sensing activity in real time during infection of brine shrimp larvae.
The ISME journal, 2012Co-Authors: Tom Defoirdt, Patrick SorgeloosAbstract:Quorum sensing, bacterial cell-to-cell communication, has been linked to the virulence of pathogenic bacteria. Indeed, in vitro experiments have shown that many bacterial pathogens regulate the expression of virulence genes by this cell-to-cell communication process. Moreover, signal molecules have been detected in samples retrieved from infected hosts and quorum sensing disruption has been reported to result in reduced virulence in different host–pathogen systems. However, data on in vivo quorum sensing activity of pathogens during infection of a host are currently lacking. We previously reported that quorum sensing regulates the virulence of Vibrio harveyi in a standardised model system with gnotobiotic brine shrimp (Artemia franciscana) larvae. Here, we monitored quorum sensing activity in Vibrio harveyi during infection of the shrimp, using bioluminescence as a read-out. We found that wild-type Vibrio harveyi shows a strong increase in quorum sensing activity early during infection. In this respect, the bacteria behave remarkably similar in different larvae, despite the fact that only half of them survive the infection. Interestingly, when expressed per bacterial cell, Vibrio harveyi showed around 200-fold higher maximal quorum sensing-regulated bioluminescence when associated with larvae than in the culture water. Finally, the in vivo quorum sensing activity of mutants defective in the production of one of the three signal molecules is consistent with their virulence, with no detectable in vivo quorum sensing activity in AI-2- and CAI-1-deficient mutants. These results indicate that AI-2 and CAI-1 are the dominant signals during infection of brine shrimp.
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Quorum quenching bacteria protect Macrobrachium rosenbergii larvae from Vibrio harveyi infection.
Journal of Applied Microbiology, 2010Co-Authors: Do Thi Thanh Nhan, Tom Defoirdt, Mathieu Wille, Peter Bossier, Patrick SorgeloosAbstract:Aims: In this study, we investigated the effect of N-acyl homoserine lactone-degrading bacterial enrichment cultures (ECs) on larviculture of the giant freshwater prawn Macrobrachium rosenbergii. Methods and Results: The larval performance in terms of larval growth, larval survival, larval quality, duration of the larval rearing process and microflora levels in the rearing water as well as inside the prawn gut was investigated. The application of the EC bacteria was performed in two ways: by adding them directly into the larval rearing water and via enriched Artemia nauplii used for larval feeding. The results of the study demonstrated that both ECs that were tested had a similar positive effect on larval survival and larval quality, whereas they did not affect larval growth or the duration of the larval rearing process. Conclusions: Under normal hatchery conditions, the optimal EC densities were found to be 106 CFU ml-1 for adding into the rearing water and 5 × 108 CFU ml-1 for enrichment of Artemia nauplii used for feeding of the larvae. In the hatchery, the ECs can be grown on waste streams of Artemia hatching. Significance and Impact of the Study: Application of this kind of ECs could lead to a more sustainable aquaculture production, by replacing the use of antibiotics to control diseases. © 2010 The Society for Applied Microbiology. Author Keywords: bacteria; enrichment cultures; Macrobrachium rosenbergii; Vibrio harveyi Index Keywords: ammonia; n acylhomoserine lactone; bacterial disease; bacterium; biodegradation; biological control; biotechnology; disease control; freshwater environment; growth rate; larval development; larviculture; prawn culture; survival; sustainable development; animal cell; animal experiment; animal model; article; bacterium culture; controlled study; dose response; enrichment culture; enteric feeding; hatchery; intestine; larva; larval development; larval stage; macrobrachium rosenbergii; metamorphosis; microflora; nonhuman; quorum sensing; shrimp; survival; toxicity testing; Vibrio harveyi; Vibriosis; Artemia; Bacteria (microorganisms); Macrobrachium rosenbergii; Vibrio harveyi
Peter Bossier - One of the best experts on this subject based on the ideXlab platform.
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Quorum sensing-disrupting compounds protect larvae of the giant freshwater prawn Macrobrachium rosenbergii from Vibrio harveyi infection
Aquaculture, 2013Co-Authors: Gde Sasmita Julyantoro Pande, Mathieu Wille, Patrick Sorgeloos, Peter Bossier, Anne Aamdal Scheie, Tore Benneche, Tom DefoirdtAbstract:Abstract Vibriosis outbreaks caused by Vibrio harveyi and related species are amongst the major obstacles for the further expansion of giant freshwater prawn ( Macrobrachium rosenbergii ) larviculture. Vibrio harveyi regulates virulence gene expression through quorum sensing, bacterial cell-to-cell communication, and consequently, quorum sensing disruption has been suggested as an alternative strategy to control infections caused by these bacteria. Previous studies have shown that quorum sensing-disrupting compounds are able to disrupt quorum sensing in Vibrio harveyi . In this study, we demonstrated that the quorum sensing-disrupting compounds cinnamaldehyde, ( Z -)-4-bromo-5-(bromomethylene)-2(5 H )-furanone and ( Z )-4-((5-(bromomethylene)-2-oxo-2,5-dihydrothiophen-3-yl)metoxy)-4-oxobutanoic acid increased the survival of giant freshwater prawn larvae when challenged to pathogenic Vibrio harveyi . Our in vivo challenge test showed that cinnamaldehyde and the thiophenone can protect the larvae from Vibrio harveyi infection when dosed to the culture water at 1 μM and 10 μM, whereas the brominated furanone offered protection at 1 μM but resulted in complete mortality at 10 μM. Although there were significant differences in survival between challenged larvae with and without addition of quorum sensing-disrupting compounds, there were no differences in growth (as determined by the larval stage index).
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Quorum quenching bacteria protect Macrobrachium rosenbergii larvae from Vibrio harveyi infection.
Journal of Applied Microbiology, 2010Co-Authors: Do Thi Thanh Nhan, Tom Defoirdt, Mathieu Wille, Peter Bossier, Patrick SorgeloosAbstract:Aims: In this study, we investigated the effect of N-acyl homoserine lactone-degrading bacterial enrichment cultures (ECs) on larviculture of the giant freshwater prawn Macrobrachium rosenbergii. Methods and Results: The larval performance in terms of larval growth, larval survival, larval quality, duration of the larval rearing process and microflora levels in the rearing water as well as inside the prawn gut was investigated. The application of the EC bacteria was performed in two ways: by adding them directly into the larval rearing water and via enriched Artemia nauplii used for larval feeding. The results of the study demonstrated that both ECs that were tested had a similar positive effect on larval survival and larval quality, whereas they did not affect larval growth or the duration of the larval rearing process. Conclusions: Under normal hatchery conditions, the optimal EC densities were found to be 106 CFU ml-1 for adding into the rearing water and 5 × 108 CFU ml-1 for enrichment of Artemia nauplii used for feeding of the larvae. In the hatchery, the ECs can be grown on waste streams of Artemia hatching. Significance and Impact of the Study: Application of this kind of ECs could lead to a more sustainable aquaculture production, by replacing the use of antibiotics to control diseases. © 2010 The Society for Applied Microbiology. Author Keywords: bacteria; enrichment cultures; Macrobrachium rosenbergii; Vibrio harveyi Index Keywords: ammonia; n acylhomoserine lactone; bacterial disease; bacterium; biodegradation; biological control; biotechnology; disease control; freshwater environment; growth rate; larval development; larviculture; prawn culture; survival; sustainable development; animal cell; animal experiment; animal model; article; bacterium culture; controlled study; dose response; enrichment culture; enteric feeding; hatchery; intestine; larva; larval development; larval stage; macrobrachium rosenbergii; metamorphosis; microflora; nonhuman; quorum sensing; shrimp; survival; toxicity testing; Vibrio harveyi; Vibriosis; Artemia; Bacteria (microorganisms); Macrobrachium rosenbergii; Vibrio harveyi
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Luminescence, virulence and quorum sensing signal production by pathogenic Vibrio campbellii and Vibrio harveyi isolates.
Journal of applied microbiology, 2007Co-Authors: Tom Defoirdt, Willy Verstraete, Peter BossierAbstract:Aims: To study the relationship between luminescence, autoinducer production and virulence of pathogenic Vibrios. Methods and Results: Luminescence, quorum sensing signal production and virulence towards brine shrimp nauplii of 13 Vibrio campbellii and Vibrio harveyi strains were studied. Although only two of the tested strains were brightly luminescent, all of them were shown to produce the three different types of quorum sensing signals known to be produced by Vibrio harveyi. Cell-free culture fluids of all strains significantly induced bioluminescence in the cholerae autoinducer 1, autoinducer 2 and harveyi autoinducer 1 reporter strains JAF375, JMH597 and JMH612, respectively. There was no relation between luminescence and signal production and virulence towards brine shrimp. Conclusions: There is a large difference between different strains of Vibrio campbellii and Vibrio harveyi with respect to bioluminescence. However, this is not reflected in signal production and virulence towards gnotobiotic brine shrimp. Moreover, there seems to be no relation between quorum sensing signal production and virulence towards brine shrimp. Significance and Impact of the Study: The results presented here indicate that strains that are most brightly luminescent are not necessarily the most virulent ones and that the lower virulence of some of the strains is not due to a lack of autoinducer production.
Iddya Karunasagar - One of the best experts on this subject based on the ideXlab platform.
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Biofilm formation by Vibrio harveyi on surfaces
Aquaculture, 1996Co-Authors: S.k. Otta, Iddya KarunasagarAbstract:Abstract The role of biofilm in the survival and persistence of the bacterial shrimp pathogen Vibrio harveyi and its possible role in perpetuating infection in shrimp hatcheries was studied. Vibrio harveyi formed biofilms on all three substrates tested: cement slab, high density polyethylene (HDPE) plastic and steel coupons. Cell density was highest on the plastic surface followed by the cement slab and the steel surface. Biofilm on the three surfaces also exhibited differential sensitivity to the sanitiser chlorine, maximum resistance being found on the concrete slab followed by plastic and steel coupons. Planktonic cells were sensitive to short exposure to low levels of chlorine. Biofilm formation occurred even in the presence of the antibiotics chloramphenicol and tetracycline, both added to the medium at 50 ppm.
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mass mortality of penaeus monodon larvae due to antibiotic resistant Vibrio harveyi infection
Aquaculture, 1994Co-Authors: Iddya Karunasagar, R Pai, G R Malathi, Iddya KarunasagarAbstract:Abstract The cause of mass mortality in Penaeus monodon larvae in a hatchery was investigated. Antibiotic-resistant Vibrio harveyi could be isolated from all the infected larvae. These bacteria were absent in healthy eggs and nauplii. Although the intake seawater had V. harveyi , these strains were sensitive to antibiotics. The results suggest that antibiotic-resistant V. harveyi had been colonising larval tanks. The isolates from moribund larvae showed much lower LD 50 values than isolates from natural seawater, thus indicating their higher virulence.
Prasanna Kumar Patil - One of the best experts on this subject based on the ideXlab platform.
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Vibrio harveyi biofilm as immunostimulant candidate for high-health pacific white shrimp, Penaeus vannamei farming.
Fish & shellfish immunology, 2019Co-Authors: T.n. Vinay, Arvind Kumar Ray, Satheesha Avunje, Sathish Kumar Thangaraj, Halesha Krishnappa, Baskaran Viswanathan, Mohan Anjaneya Reddy, Koyadan Kizhakedath Vijayan, Prasanna Kumar PatilAbstract:Abstract The study was to develop Vibrio harveyi biofilm-based novel microbial product and its oral delivery for high health Penaeus vannamei farming. Yield of bacterial biofilm was optimized on chitin substrate (size:
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Vibrio harveyi biofilm as immunostimulant candidate for high-health pacific white shrimp, Penaeus vannamei farming
'Elsevier BV', 2019Co-Authors: Vinay Tharabenahalli Nagaraju, Arvind Kumar Ray, Satheesha Avunje, Halesha Krishnappa, Baskaran Viswanathan, Mohan Anjaneya Reddy, Satish Kumar T, Vijayan Koyadan Kizhakedath, Prasanna Kumar PatilAbstract:Not AvailableThe study was to develop Vibrio harveyi biofilm-based novel microbial product and its oral delivery for high health Penaeus vannamei farming. Yield of bacterial biofilm was optimized on chitin substrate (size:
