The Experts below are selected from a list of 162 Experts worldwide ranked by ideXlab platform
Shuji Tanaka - One of the best experts on this subject based on the ideXlab platform.
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cloning and sequence analysis of vibrio parahaemolyticus ompk gene encoding a 26 kda outer membrane protein ompk that serves as receptor for a broad host range Vibriophage kvp40
Fems Microbiology Letters, 1995Co-Authors: Tetsuyoshi Inoue, Shigenobu Matsuzaki, Shuji TanakaAbstract:The ompK gene of Vibrio parahaemolyticus 1010 (RIMD 2210001) encoding an outer membrane protein (OMP), OmpK, which serves as the receptor for a broad-host-range Vibriophage, KVP40, was cloned and sequenced. The gene consisted of 789 nucleotides encoding 263 amino acids. Since the first 20 amino acids most likely constitute the signal peptide, mature OmpK would consist of 243 amino acids with a calculated molecular mass of 27458 Da. Sequence comparisons indicate that OmpK is unique among Vibrio OMPs so far sequenced, but may be distantly related to Tsx of enteric bacteria and is homologous to an Aeromonas hydrophila OMP, protein IV.
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a 26 kda outer membrane protein ompk common to vibrio species is the receptor for a broad host range Vibriophage kvp40
Fems Microbiology Letters, 1995Co-Authors: Tetsuyoshi Inoue, Shigenobu Matsuzaki, Shuji TanakaAbstract:KVP40 is a broad-host-range Vibriophage forming plaques on strains of at least eight Vibrio and one Photobacterium species. A spontaneous KVP40-resistant mutant, R4000, derived from Vibrio parahaemolyticus 1010 lacked a 26-kDa outer membrane protein designated OmpK. KVP40 was inactivated by outer membrane and OmpK prepared from 1010, but not by outer membrane from R4000. These results strongly suggest that OmpK is the receptor for KVP40. Immunoblotting analyses using an anti-OmpK rabbit serum revealed that OmpK or its homologs of molecular masses 25–29 kDa were distributed widely among Vibrio and Photobacterium strains including those naturally resistant to KVP40.
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evidence for the existence of a restriction modification system common to several species of the family vibrionaceae
Fems Microbiology Letters, 1992Co-Authors: Shigenobu Matsuzaki, Tetsuyoshi Inoue, Shuji TanakaAbstract:A broad-host-range Vibriophage KVP40 originally isolated on Vibrio parahaemolyticus 1010 was restricted and modified by strains of at least five Vibrio and one Photobacterium species. 1010 was a non-restricting host. An anti-restriction mutant KVP40 aar 1 was isolated after propagating the phage on a restricting host, V. anguillarum VIB36. KVP40 aar 1 grown on either 1010 or VIB36, as well as the parental phage grown on VIB36, showed much higher efficiencies of plating on all the restricting hosts as compared with the parental phage grown on 1010, indicating that these restricting hosts probably share a common restriction-modification system active in vivo on KVP40.
Tetsuyoshi Inoue - One of the best experts on this subject based on the ideXlab platform.
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cloning and sequence analysis of vibrio parahaemolyticus ompk gene encoding a 26 kda outer membrane protein ompk that serves as receptor for a broad host range Vibriophage kvp40
Fems Microbiology Letters, 1995Co-Authors: Tetsuyoshi Inoue, Shigenobu Matsuzaki, Shuji TanakaAbstract:The ompK gene of Vibrio parahaemolyticus 1010 (RIMD 2210001) encoding an outer membrane protein (OMP), OmpK, which serves as the receptor for a broad-host-range Vibriophage, KVP40, was cloned and sequenced. The gene consisted of 789 nucleotides encoding 263 amino acids. Since the first 20 amino acids most likely constitute the signal peptide, mature OmpK would consist of 243 amino acids with a calculated molecular mass of 27458 Da. Sequence comparisons indicate that OmpK is unique among Vibrio OMPs so far sequenced, but may be distantly related to Tsx of enteric bacteria and is homologous to an Aeromonas hydrophila OMP, protein IV.
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a 26 kda outer membrane protein ompk common to vibrio species is the receptor for a broad host range Vibriophage kvp40
Fems Microbiology Letters, 1995Co-Authors: Tetsuyoshi Inoue, Shigenobu Matsuzaki, Shuji TanakaAbstract:KVP40 is a broad-host-range Vibriophage forming plaques on strains of at least eight Vibrio and one Photobacterium species. A spontaneous KVP40-resistant mutant, R4000, derived from Vibrio parahaemolyticus 1010 lacked a 26-kDa outer membrane protein designated OmpK. KVP40 was inactivated by outer membrane and OmpK prepared from 1010, but not by outer membrane from R4000. These results strongly suggest that OmpK is the receptor for KVP40. Immunoblotting analyses using an anti-OmpK rabbit serum revealed that OmpK or its homologs of molecular masses 25–29 kDa were distributed widely among Vibrio and Photobacterium strains including those naturally resistant to KVP40.
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evidence for the existence of a restriction modification system common to several species of the family vibrionaceae
Fems Microbiology Letters, 1992Co-Authors: Shigenobu Matsuzaki, Tetsuyoshi Inoue, Shuji TanakaAbstract:A broad-host-range Vibriophage KVP40 originally isolated on Vibrio parahaemolyticus 1010 was restricted and modified by strains of at least five Vibrio and one Photobacterium species. 1010 was a non-restricting host. An anti-restriction mutant KVP40 aar 1 was isolated after propagating the phage on a restricting host, V. anguillarum VIB36. KVP40 aar 1 grown on either 1010 or VIB36, as well as the parental phage grown on VIB36, showed much higher efficiencies of plating on all the restricting hosts as compared with the parental phage grown on 1010, indicating that these restricting hosts probably share a common restriction-modification system active in vivo on KVP40.
Shigenobu Matsuzaki - One of the best experts on this subject based on the ideXlab platform.
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sequence analysis of bacteriophage t4 dna packaging terminase genes 16 and 17 reveals a common atpase center in the large subunit of viral terminases
Nucleic Acids Research, 2002Co-Authors: Michael S Mitchell, Shigenobu Matsuzaki, Shosuke Imai, V. B. RaoAbstract:Phage DNA packaging is believed to be driven by a rotary device coupled to an ATPase ‘motor’. Recent evidence suggests that the phage DNA packaging motor is one of the strongest force-generating molecular motors reported to date. However, the ATPase center that is responsible for generating this force is unknown. In order to identify the DNA translocating ATPase, the sequences of the packaging/terminase genes of coliphages T4 and RB49 and Vibriophages KVP40 and KVP20 have been analyzed. Alignment of the terminase polypeptide sequences revealed a number of functional signatures in the terminase genes 16 and 17. Most importantly, the data provide compelling evidence for an ATPase catalytic center in the N-terminal half of the large terminase subunit gp17. An analogous ATPase domain consisting of conserved functional signatures is also identified in the large terminase subunit of other bacteriophages and herpesviruses. Interestingly, the putative terminase ATPase domain exhibits some of the common features found in the ATPase domain of DEAD box helicases. Residues that would be critical for ATPase catalysis and its coupling to DNA packaging are identified. Com binatorial mutagenesis shows that the predicted threonine residues in the putative ATPase coupling motif are indeed critical for function.
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cloning and sequence analysis of vibrio parahaemolyticus ompk gene encoding a 26 kda outer membrane protein ompk that serves as receptor for a broad host range Vibriophage kvp40
Fems Microbiology Letters, 1995Co-Authors: Tetsuyoshi Inoue, Shigenobu Matsuzaki, Shuji TanakaAbstract:The ompK gene of Vibrio parahaemolyticus 1010 (RIMD 2210001) encoding an outer membrane protein (OMP), OmpK, which serves as the receptor for a broad-host-range Vibriophage, KVP40, was cloned and sequenced. The gene consisted of 789 nucleotides encoding 263 amino acids. Since the first 20 amino acids most likely constitute the signal peptide, mature OmpK would consist of 243 amino acids with a calculated molecular mass of 27458 Da. Sequence comparisons indicate that OmpK is unique among Vibrio OMPs so far sequenced, but may be distantly related to Tsx of enteric bacteria and is homologous to an Aeromonas hydrophila OMP, protein IV.
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a 26 kda outer membrane protein ompk common to vibrio species is the receptor for a broad host range Vibriophage kvp40
Fems Microbiology Letters, 1995Co-Authors: Tetsuyoshi Inoue, Shigenobu Matsuzaki, Shuji TanakaAbstract:KVP40 is a broad-host-range Vibriophage forming plaques on strains of at least eight Vibrio and one Photobacterium species. A spontaneous KVP40-resistant mutant, R4000, derived from Vibrio parahaemolyticus 1010 lacked a 26-kDa outer membrane protein designated OmpK. KVP40 was inactivated by outer membrane and OmpK prepared from 1010, but not by outer membrane from R4000. These results strongly suggest that OmpK is the receptor for KVP40. Immunoblotting analyses using an anti-OmpK rabbit serum revealed that OmpK or its homologs of molecular masses 25–29 kDa were distributed widely among Vibrio and Photobacterium strains including those naturally resistant to KVP40.
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evidence for the existence of a restriction modification system common to several species of the family vibrionaceae
Fems Microbiology Letters, 1992Co-Authors: Shigenobu Matsuzaki, Tetsuyoshi Inoue, Shuji TanakaAbstract:A broad-host-range Vibriophage KVP40 originally isolated on Vibrio parahaemolyticus 1010 was restricted and modified by strains of at least five Vibrio and one Photobacterium species. 1010 was a non-restricting host. An anti-restriction mutant KVP40 aar 1 was isolated after propagating the phage on a restricting host, V. anguillarum VIB36. KVP40 aar 1 grown on either 1010 or VIB36, as well as the parental phage grown on VIB36, showed much higher efficiencies of plating on all the restricting hosts as compared with the parental phage grown on 1010, indicating that these restricting hosts probably share a common restriction-modification system active in vivo on KVP40.
Amar N. Ghosh - One of the best experts on this subject based on the ideXlab platform.
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Structural analysis and proteomics studies on the Myoviridae Vibriophage M4
Archives of Virology, 2019Co-Authors: Moumita Dutta, Amar N. GhoshAbstract:Bacteriophages play a crucial role in tracking the spread of bacterial epidemics. The frequent emergence of antibiotic-resistant bacterial strains throughout the world has motivated studies on bacteriophages that can potentially be used in phage therapy as an alternative to conventional antibiotic treatment. A recent outbreak of cholera in Haiti took many lives due to a rapid development of resistance to the available antibiotics. The properties of Vibriophages, bacteriophages that infect Vibrio cholerae , are therefore of practical interest. A detailed understanding of the structure and assembly of a Vibriophage is potentially useful in developing phage therapy against cholera as well as for fabricating artificial nanocontainers. Therefore, the aim of the present study was to determine the three-dimensional organization of Vibriophage M4 at sub-nanometer resolution by electron microscopy and single-particle analysis techniques to facilitate its use as a therapeutic agent. We found that M4 has a large capsid with T = 13 icosahedral symmetry and a long contractile tail. This double-stranded DNA phage also contains a head-to-tail connector protein complex that joins the capsid to the tail and a prominent baseplate at the end of the tail. This study also provides information regarding the proteome of this phage, which is proteins similar to that of other Myoviridae phages, and most of the encoded proteins are structural proteins that form the exquisite architecture of this bacteriophage.
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visualizing a vibrio cholerae o1 el tor typing bacteriophage belonging to the myoviridae group and the packaging of its genomic ends inside the phage capsid
Journal of Biomolecular Structure & Dynamics, 2018Co-Authors: Anindito Sen, Amar N. GhoshAbstract:Phage D10, an O1 El Tor tying Vibriophage, has been successfully employed to tract the outspread of cholera epidemic. Using Transmission Electron Microscopy and computational image analysis, we hav...
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Visualizing a Vibrio cholerae O1 El Tor typing bacteriophage belonging to the Myoviridae group and the packaging of its genomic ends inside the phage capsid
2017Co-Authors: Anindito Sen, Amar N. GhoshAbstract:Phage D10, an O1 El Tor tying Vibriophage, has been successfully employed to tract the outspread of cholera epidemic. Using Transmission Electron Microscopy and computational image analysis, we have determined the structures of the capsid, head-to-tail connector, the contractile helical tail, the baseplate and combined them to form the complete three-dimensional (3D) D10 phage structure. Using partial denaturation experiments on the genome and using the computed 3D structure of the phage, we have established the packing of the genome ends inside the capsid together with the release styles during the phage infection, respectively. Finally, using the 3D density maps of the different components of the D10 phage, we have presented a simplified picture of morphogenesis of the D10 Vibriophage. Using the complete assembled structure of the D10 phage, we have traced the path of the phage genome during the infection process, all the way from the phage head down the tail tube of the tail to the top of the baseplate. To the best of our knowledge, this is first structural study for a long-tailed Vibriophage. We have tabulated the structural features of the different components of the phages belonging to the Myoviridae and Siphoviridae. The comparative study suggested the possibility of a common origin of the bacteriophages, irrespective of belonging to different groups and species.
Mathias Middelboe - One of the best experts on this subject based on the ideXlab platform.
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stumbling across the same phage comparative genomics of widespread temperate phages infecting the fish pathogen vibrio anguillarum
Viruses, 2017Co-Authors: Panos G Kalatzis, Nanna Rorbo, Daniel Castillo, Jesper Juel Mauritzen, Johanna Jorgensen, Constantina Kokkari, Faxing Zhang, Pantelis Katharios, Mathias MiddelboeAbstract:Nineteen Vibrio anguillarum-specific temperate bacteriophages isolated across Europe and Chile from aquaculture and environmental sites were genome sequenced and analyzed for host range, morphology and life cycle characteristics. The phages were classified as Siphoviridae with genome sizes between 46,006 and 54,201 bp. All 19 phages showed high genetic similarity, and 13 phages were genetically identical. Apart from sporadically distributed single nucleotide polymorphisms (SNPs), genetic diversifications were located in three variable regions (VR1, VR2 and VR3) in six of the phage genomes. Identification of specific genes, such as N6-adenine methyltransferase and lambda like repressor, as well as the presence of a tRNAArg, suggested a both mutualistic and parasitic interaction between phages and hosts. During short term phage exposure experiments, 28% of a V. anguillarum host population was lysogenized by the temperate phages and a genomic analysis of a collection of 31 virulent V. anguillarum showed that the isolated phages were present as prophages in >50% of the strains covering large geographical distances. Further, phage sequences were widely distributed among CRISPR-Cas arrays of publicly available sequenced Vibrios. The observed distribution of these specific temperate Vibriophages across large geographical scales may be explained by efficient dispersal of phages and bacteria in the marine environment combined with a mutualistic interaction between temperate phages and their hosts which selects for co-existence rather than arms race dynamics.
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Vibriophages differentially influence biofilm formation by vibrio anguillarum strains
Applied and Environmental Microbiology, 2015Co-Authors: Demeng Tan, Amalie Dahl, Mathias MiddelboeAbstract:Vibrio anguillarum is an important pathogen in marine aquaculture, responsible for vibriosis. Bacteriophages can potentially be used to control bacterial pathogens; however, successful application of phages requires a detailed understanding of phage-host interactions under both free-living and surface-associated growth conditions. In this study, we explored in vitro phage-host interactions in two different strains of V. anguillarum (BA35 and PF430-3) during growth in microcolonies, biofilms, and free-living cells. Two Vibriophages, ΦH20 (Siphoviridae) and KVP40 (Myoviridae), had completely different effects on the biofilm development. Addition of phage ΦH20 to strain BA35 showed efficient control of biofilm formation and density of free-living cells. The interactions between BA35 and ΦH20 were thus characterized by a strong phage control of the phage-sensitive population and subsequent selection for phage-resistant mutants. Addition of phage KVP40 to strain PF430-3 resulted in increased biofilm development, especially during the early stage. Subsequent experiments in liquid cultures showed that addition of phage KVP40 stimulated the aggregation of host cells, which protected the cells against phage infection. By the formation of biofilms, strain PF430-3 created spatial refuges that protected the host from phage infection and allowed coexistence between phage-sensitive cells and lytic phage KVP40. Together, the results demonstrate highly variable phage protection mechanisms in two closely related V. anguillarum strains, thus emphasizing the challenges of using phages to control vibriosis in aquaculture and adding to the complex roles of phages as drivers of prokaryotic diversity and population dynamics.
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Vibriophages and their interactions with the fish pathogen vibrio anguillarum
Applied and Environmental Microbiology, 2014Co-Authors: Demeng Tan, Lone Gram, Mathias MiddelboeAbstract:Vibrio anguillarum is an important pathogen in aquaculture, responsible for the disease vibriosis in many fish and invertebrate species. Disease control by antibiotics is a concern due to potential development and spread of antibiotic resistance. The use of bacteriophages to control the pathogen may offer a non-antibiotic-based approach to reduce vibriosis. A detailed understanding of the phage-host interaction is needed to evaluate the potential of phages to control the pathogen. In this study, we examined the diversity and interactions of 11 Vibriophages, 24 V. anguillarum strains, and 13 Vibrio species strains. Together, the host ranges of the 11 phages covered all of the tested 37 Vibrio sp. host strains, which represented considerable temporal (20 years) and geographical (9 countries) differences in their origins of isolation. Thus, despite the occurrence of unique susceptibility patterns of the individual host isolates, key phenotypic properties related to phage susceptibility are distributed worldwide and maintained in the global Vibrio community for decades. The phage susceptibility pattern of the isolates did not show any relation to the physiological relationships obtained from Biolog GN2 profiles, demonstrating that similar phage susceptibility patterns occur across broad phylogenetic and physiological differences in Vibrio strains. Subsequent culture experiments with two phages and two V. anguillarum hosts demonstrated an initial strong lytic potential of the phages. However, rapid regrowth of both phage-resistant and phage-sensitive cells following the initial lysis suggested that several mechanisms of protection against phage infection had developed in the host populations.
