The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
F. Ascencio - One of the best experts on this subject based on the ideXlab platform.
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Experimental Vibriosis Induction with Vibrio alginolyticus of Larvae of the Catarina Scallop (Argopecten ventricosus = circularis) (Sowerby II, 1842)
Microbial ecology, 1998Co-Authors: J.c. Sainz, A.n. Maeda-martínez, F. AscencioAbstract:Six-day-old larvae of the catarina scallop, Argopecten ventricosus (=circularis), were infected with different concentrations of Vibrio alginolyticus to determine virulence and to describe Vibriosis in this species. The development of Vibriosis was compared to the effect of the supernatant of a 24-h V. alginolyticus culture. An experimental larvae culture system (ELCS) yielded a maximum survival of 80% from the 6th to the 19th day (control and low concentrations of V. alginolyticus). No effect was shown with concentrations of V. alginolyticus below 0.5 × 105 CFU ml−1. At concentrations higher than 5.0 × 105 CFU ml−1, swimming depletion, empty stomachs, lipidic granules in the digestive system, velum degradation, and massive mortality were observed. The supernatant of V. alginolyticus culture showed similar effects to the highest concentrations of V. alginolyticus cells.
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experimental Vibriosis induction with vibrio alginolyticus of larvae of the catarina scallop argopecten ventricosus circularis sowerby ii 1842
Microbial Ecology, 1998Co-Authors: J.c. Sainz, A N Maedamartinez, F. AscencioAbstract:Six-day-old larvae of the catarina scallop, Argopecten ventricosus (=circularis), were infected with different concentrations of Vibrio alginolyticus to determine virulence and to describe Vibriosis in this species. The development of Vibriosis was compared to the effect of the supernatant of a 24-h V. alginolyticus culture. An experimental larvae culture system (ELCS) yielded a maximum survival of 80% from the 6th to the 19th day (control and low concentrations of V. alginolyticus). No effect was shown with concentrations of V. alginolyticus below 0.5 × 105 CFU ml−1. At concentrations higher than 5.0 × 105 CFU ml−1, swimming depletion, empty stomachs, lipidic granules in the digestive system, velum degradation, and massive mortality were observed. The supernatant of V. alginolyticus culture showed similar effects to the highest concentrations of V. alginolyticus cells.
J.c. Sainz - One of the best experts on this subject based on the ideXlab platform.
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Experimental Vibriosis Induction with Vibrio alginolyticus of Larvae of the Catarina Scallop (Argopecten ventricosus = circularis) (Sowerby II, 1842)
Microbial ecology, 1998Co-Authors: J.c. Sainz, A.n. Maeda-martínez, F. AscencioAbstract:Six-day-old larvae of the catarina scallop, Argopecten ventricosus (=circularis), were infected with different concentrations of Vibrio alginolyticus to determine virulence and to describe Vibriosis in this species. The development of Vibriosis was compared to the effect of the supernatant of a 24-h V. alginolyticus culture. An experimental larvae culture system (ELCS) yielded a maximum survival of 80% from the 6th to the 19th day (control and low concentrations of V. alginolyticus). No effect was shown with concentrations of V. alginolyticus below 0.5 × 105 CFU ml−1. At concentrations higher than 5.0 × 105 CFU ml−1, swimming depletion, empty stomachs, lipidic granules in the digestive system, velum degradation, and massive mortality were observed. The supernatant of V. alginolyticus culture showed similar effects to the highest concentrations of V. alginolyticus cells.
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experimental Vibriosis induction with vibrio alginolyticus of larvae of the catarina scallop argopecten ventricosus circularis sowerby ii 1842
Microbial Ecology, 1998Co-Authors: J.c. Sainz, A N Maedamartinez, F. AscencioAbstract:Six-day-old larvae of the catarina scallop, Argopecten ventricosus (=circularis), were infected with different concentrations of Vibrio alginolyticus to determine virulence and to describe Vibriosis in this species. The development of Vibriosis was compared to the effect of the supernatant of a 24-h V. alginolyticus culture. An experimental larvae culture system (ELCS) yielded a maximum survival of 80% from the 6th to the 19th day (control and low concentrations of V. alginolyticus). No effect was shown with concentrations of V. alginolyticus below 0.5 × 105 CFU ml−1. At concentrations higher than 5.0 × 105 CFU ml−1, swimming depletion, empty stomachs, lipidic granules in the digestive system, velum degradation, and massive mortality were observed. The supernatant of V. alginolyticus culture showed similar effects to the highest concentrations of V. alginolyticus cells.
Carmen Amaro - One of the best experts on this subject based on the ideXlab platform.
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Vaccination of market-size eels against Vibriosis due to Vibrio vulnificus serovar E
Aquaculture, 2004Co-Authors: Maria D. Esteve-gassent, Rodolfo Barrera, Carmen AmaroAbstract:Vaccination with Vulnivaccine at eel farms has been previously shown to protect cultured eels against Vibriosis caused by Vibrio vulnificus serovar E for more than 1 year. The reported protocol included an initial vaccination by triple prolonged immersion at the glass-eel stage together with one optional oral booster at the elver stage. However, eels at the market-size stage (around 150 g body weight) can suffer stress-related Vibriosis after handling and transport to the selling facilities, which implies a serious risk for consumer health. The main objective of this work was therefore to develop an effective re-vaccination procedure, useful for preventing stress-related Vibriosis and zoonosis in market-size eels. To this end, eels, primed 2 years before, were reimmunised by prolonged immersion with a very low dose of Vulnivaccine, a procedure commercially applicable to large fish. The protection, the immune response in plasma, skin mucus and bile as well as the bactericidal activity of the collected body fluids were evaluated in reimmunised and control animals (non-reimmunised) during a 30-day period. Reimmunisation significantly increased antibody titres in plasma, mucus and bile, as well as the bactericidal activity of these fluids, and reduced the mortality rate after bath challenge with the pathogen. In conclusion, the prolonged immersion in a very high dilution of Vulnivaccine can protect market-size eels against stress-related Vibriosis and, consequently, significantly reduce additional risks of zoonosis.
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Susceptibility of Nile tilapia (Oreochromis niloticus) to Vibriosis due to Vibrio vulnificus biotype 2 (serovar E)
Aquaculture, 2002Co-Authors: Belén Fouz, Rodolfo Barrera, Elena Alcaide, Carmen AmaroAbstract:The present study documents the susceptibility of Nile tilapia to the experimental Vibriosis caused by Vibrio vulnificus biotype 2 (serovar E) using a reference strain (Spanish Collection of Type Cultures, CECT 4604) selected for its high degree of virulence for eels. The biotype 1 of this species is one of the usual organisms involved in epizootics occurred in tilapia. After intraperitoneal injection, the selected strain developed a haemorrhagic septicaemia similar to eel Vibriosis with a LD50 four log units lower than that exhibited by the type strain of the species, which belongs to the biotype 1. The results obtained in waterborne and intubation challenges indicated that water and feed could act as a vehicle for Vibriosis transmission to healthy tilapia. Moreover, live cells and the extracellular products derived from the strain CECT 4604 showed remarkable activity against tilapia erythrocytes, which correlated with the in vivo production of extensive haemorrhagic areas. Our results suggest that this bacterium could constitute a serious health hazard for tilapia, especially if it is cocultured with eels. Thus, vaccination of tilapia with a vaccine against V. vulnificus biotype 2 could be the best strategy to prevent any cross transmission of the disease from eels to tilapia under intensive rearing conditions.
Gabriela Gaxiola - One of the best experts on this subject based on the ideXlab platform.
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A Vibriosis outbreak in the Pacific white shrimp, Litopenaeus vannamei reared in biofloc and clear seawater.
Journal of invertebrate pathology, 2019Co-Authors: Diana Aguilera-rivera, Alejandra Prieto-davó, Gabriela Rodríguez-fuentes, Karla S. Escalante-herrera, Gabriela GaxiolaAbstract:In May and June 2015, moderate and severe lesions were observed in Litopenaeus vannamei reared in clear seawater while, at the same time, lesions in shrimp reared in biofloc were considerably fewer. The signs of disease included anorexia, lethargy, melanization, expanded chromatophores, luminescence and necrotic areas in the uropods, suggesting a possible Vibriosis. However, lesions observed in shrimp reared in biofloc disappeared after a certain time and without mortality in tanks, whereas mortality and severe signs continued to be observed in shrimp reared in clear seawater. To treat the possible Vibriosis, oxytetracycline was administered only in clear seawater tanks, but the results were not successful. Bacterial cultures from hepatopancreas tissues of shrimp from both rearing systems confirmed a Vibriosis outbreak only in the clear seawater system. Subsequently, Vibrio harveyi, Vibrio rotiferianus, Photobacterium sp. and Photobacterium damselae were identified from bacterial culture previously isolated for both rearing systems by molecular methods. Shewanella sp. was isolated and identified only in biofloc. To understand the possible pathogenicity and resistance mechanisms of the Vibiro strains for both rearing systems, pathogenicity (toxR) and oxytetracycline resistance-related genes (tet(B), tet(D), tet(G)) were determined. Although these genes were expressed for both rearing systems, biofloc proved to have the ability to control the development of the disease, in comparison to clear water, where the Vibriosis was evident regardless of the administration of oxytetracycline as a treatment.
Diana Aguilera-rivera - One of the best experts on this subject based on the ideXlab platform.
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A Vibriosis outbreak in the Pacific white shrimp, Litopenaeus vannamei reared in biofloc and clear seawater.
Journal of invertebrate pathology, 2019Co-Authors: Diana Aguilera-rivera, Alejandra Prieto-davó, Gabriela Rodríguez-fuentes, Karla S. Escalante-herrera, Gabriela GaxiolaAbstract:In May and June 2015, moderate and severe lesions were observed in Litopenaeus vannamei reared in clear seawater while, at the same time, lesions in shrimp reared in biofloc were considerably fewer. The signs of disease included anorexia, lethargy, melanization, expanded chromatophores, luminescence and necrotic areas in the uropods, suggesting a possible Vibriosis. However, lesions observed in shrimp reared in biofloc disappeared after a certain time and without mortality in tanks, whereas mortality and severe signs continued to be observed in shrimp reared in clear seawater. To treat the possible Vibriosis, oxytetracycline was administered only in clear seawater tanks, but the results were not successful. Bacterial cultures from hepatopancreas tissues of shrimp from both rearing systems confirmed a Vibriosis outbreak only in the clear seawater system. Subsequently, Vibrio harveyi, Vibrio rotiferianus, Photobacterium sp. and Photobacterium damselae were identified from bacterial culture previously isolated for both rearing systems by molecular methods. Shewanella sp. was isolated and identified only in biofloc. To understand the possible pathogenicity and resistance mechanisms of the Vibiro strains for both rearing systems, pathogenicity (toxR) and oxytetracycline resistance-related genes (tet(B), tet(D), tet(G)) were determined. Although these genes were expressed for both rearing systems, biofloc proved to have the ability to control the development of the disease, in comparison to clear water, where the Vibriosis was evident regardless of the administration of oxytetracycline as a treatment.
