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Gerard Duc - One of the best experts on this subject based on the ideXlab platform.
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no red blood cell damage and no hemolysis in g6pd deficient subjects after ingestion of low vicine convicine Vicia faba seeds
Blood, 2018Co-Authors: Valentina Gallo, Oleksii A Skorokhod, Luigi Felice Simula, Tiziana Marrocco, Elisa Tambini, Evelin Schwarzer, Pascal Marget, Gerard Duc, Paolo AreseAbstract:TO THE EDITOR: Favism, or “favic crisis,” is a potentially life-threatening acute hemolysis elicited in carriers of low-activity glucose 6-phosphate dehydrogenase (G6PD) variants by ingestion of raw faba bean ( Vicia faba L) (FB) seeds.[1][1],[2][2] In 2 surveys of hemolytic crises because of
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faba bean Vicia faba l
Field Crops Research, 1997Co-Authors: Gerard DucAbstract:Abstract This review underlines the wide genetic variability which has been collected, characterized, and sometimes induced through mutagenesis in Vicia faba L. The comprehensive knowledge collected in the scientific community during the last 30 years has permitted major advances in faba bean breeding. These include control of major biotic and abiotic stresses, and diverse seed quality aspects. Biotechnology approaches have been developed with this species and complex breeding schemes that employ male sterility to increase outcrossing have been proposed.
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a mutation in Vicia faba results in ineffective nodules with impaired bacteroid differentiation and reduced synthesis of late nodulins
Journal of Experimental Botany, 1992Co-Authors: Annette Haser, Gerard Duc, David Lowell Robinson, Carroli P VanceAbstract:Although numerous reports have documented the effect of bacterially-induced ineffectiveness on root nodule structure, function, and plant gene expression, few studies have detailed the effect of the plant genome on similar parameters. In this report effective (N 2 -fixing) broadbean (Vicia faba L.) and plant-controlled ineffectivc (non-N 2 -fixing) broadbenn recessive for the sym-1 gene were compared for nodule structure, developmental expression of nodule enzyme activities, enzyme proteins, and mRNAs involved in a assimilation, leghemoglobin (Lb) synthesis, and acetylene reduction activity (ARA)
Alfred Pühler - One of the best experts on this subject based on the ideXlab platform.
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Analysis of genes encoding modular nodulins from Vicia hirsuta and Vicia faba
Plant Science, 1999Co-Authors: Helge Küster, Alfred PühlerAbstract:Abstract The expression of genes encoding modular nodulins was analysed in the Vicia hirsuta and the Vicia faba system. From V. hirsuta root nodules, we isolated two families of transcript sequences designated VhNOD28/32-A and VhNOD28/32-B encoding nodulins homologous to the V. faba nodulins Nvf-28/32 and the Medicago sativa nodulin-25. The modular proteins encoded by the V. hirsuta transcript sequences consisted of N- and C-terminal unique modules flanking two types of repetitive modules. Specific repetitive modules were deleted from individual VhNOD28/32 transcripts. Southern hybridizations indicated the presence of either a small VhNOD28/32 gene family or a single copy gene comprising at least two alleles. VhNOD28/32 transcripts were expressed specifically in root nodules, where they were localized in the nitrogen-fixing zone III. In the Vicia faba system, we isolated the promoter of two alleles of the VfNOD28/32 gene by PCR-based DNA walking. The promoter sequences were similar to the promoter of the M. sativa nodulin-25 gene and contained an organ-specific element at position −68/−55 from the transcriptional start. An analysis of transgenic V. hirsut a root nodules expressing the gus Aint reporter gene under the control of the two VfNOD28/32 promoters demonstrated that both promoters were active in the central region of transgenic V . hirsuta nodules.
Richard C Crain - One of the best experts on this subject based on the ideXlab platform.
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abscisic acid induced phosphoinositide turnover in guard cell protoplasts of Vicia faba
Plant Physiology, 1996Co-Authors: Yunjung Choi, J F Kelleher, Sarah M. Assmann, Richard C CrainAbstract:Guard cell protoplasts of Vicia faba treated with 10 [mu]M (+)abscisic acid (ABA) in the light exhibited a 20% decrease in diameter within 1.5 h, from 24.1 to 19.6 [mu]m. Within 10 s of administration of ABA, a 90% increase in levels of inositol 1,4,5-trisphosphate was observed, provided that cells were treated with Li+, an inhibitor of inositol phosphatase activity, prior to incubation. Concomitantly, levels of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate decreased 20% compared to levels in control cells; levels of label in the membrane lipids phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol did not change significantly in response to ABA treatment. These results show that phosphoinositide turnover is activated in response to ABA in guard cells. We conclude that phosphoinositide signaling is likely to be a step in the biochemical cascade that couples ABA to guard cell shrinking and stomatal closure.
Eric Pinelli - One of the best experts on this subject based on the ideXlab platform.
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Effect of fulvic acids on lead-induced oxidative stress to metal sensitive Vicia faba L. plant
Biology and Fertility of Soils, 2012Co-Authors: Muhammad Shahid, Camille Dumat, Jérôme Silvestre, Eric PinelliAbstract:Lead (Pb) is a ubiquitous environmental pollutant capable to induce various morphological, physiological, and biochemical functions in plants. Only few publications focus on the influence of Pb speciation both on its phytoavailability and phytotoxicity. Therefore, Pb toxicity (in terms of lipid peroxidation, hydrogen peroxide induction, and photosynthetic pigments contents) was studied in Vicia faba plants in relation with Pb uptake and speciation. V. faba seedlings were exposed to Pb supplied as Pb(NO3)2 or complexed by two fulvic acids (FAs), i.e. Suwannee River fulvic acid (SRFA) and Elliott Soil fulvic acid (ESFA), for 1, 12, and 24 h under controlled hydroponic conditions. For both FAs, Pb uptake and translocation by Vicia faba increased at low level (5 mg l−1), whereas decreased at high level of application (25 mg l−1). Despite the increased Pb uptake with FAs at low concentrations, there was no influence on the Pb toxicity to the plants. However, at high concentrations, FAs reduced Pb toxicity by reducing its uptake. These results highlighted the role of the dilution factor for FAs reactivity in relation with structure; SRFA was more effective than ESFA in reducing Pb uptake and alleviating Pb toxicity to V. faba due to comparatively strong binding affinity for the heavy metal.
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assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test
Ecotoxicology and Environmental Safety, 2012Co-Authors: Ahmed Khadra, Eric Pinelli, Marlene Z Lacroix, Alain Bousquetmelou, Hassan Hamdi, Georges MerlinaAbstract:The genotoxicity of quinolone and fluroquinolones was assessed using the micronucleus (MN) test on Vicia faba roots by direct contact exposure to a solid matrix. Plants were exposed to quinolones (nalidixic acid) and fluoroquinolones (ciprofloxacin and enrofloxacin) alone or mixed with artificially contaminated soils. Four different concentrations of each of these antibiotics were tested (0.01, 0.1, 1 and 10 mg/Kg) for nalidixic acid and (0.005, 0.05, 0.5 and 5 mg/Kg) for ciprofloxacin and enrofloxacin. These antibiotics were also used in mixture. Exposure of Vicia faba plants to each antibiotic at the highest two concentrations showed significant MN induction. The lowest two concentrations had no significant genotoxic effect. The mixture of the three compounds induced a significant MN induction whatever the mixture tested, from 0.02 to 20 mg/Kg. The results indicated that a similar genotoxic effect was obtained with the mixture at 0.2 mg/Kg in comparison with each molecule alone at 5-10 mg/Kg. Data revealed a clear synergism of these molecules on Vicia faba genotoxicity.
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assessment of the genotoxicity of olive mill waste water omww with the Vicia faba micronucleus test
Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2007Co-Authors: El H Hajjouji, Eric Pinelli, Georges Merlina, Maritxu Guiresse, Jeanclaude Revel, Mohamed HafidiAbstract:The present study concerns the genotoxicity of olive mill waste water (OMWW) generated in mills producing olive oil in Morocco. The Vicia faba micronucleus test was used to evaluate the genotoxicity of OMWW and the six major phenolic compounds identified by HPLC in this effluent. Five dilutions of OMWW were tested: 0.1, 1, 5, 10 and 20%. Maleic hydrazide was used as a positive control. The results showed that OMWW was genotoxic at 10% dilution. In order to investigate the components involved in this genotoxicity, the six major phenols present in this effluent, oleuropein, gallic acid, 4-hydroxyphenyl acetic acid, caffeic acid, paracoumaric acid and veratric acid, were studied at concentrations corresponding to the genotoxic concentration of the OMWW itself. Two phenols, gallic acid and oleuropein induced a significant increase in micronucleus frequency in Vicia faba; the four other phenols had no significant genotoxic effect. These results suggest that under the experimental conditions of our assay, OMWW genotoxicity was associated with gallic acid and oleuropein.
Noriaki Kondo - One of the best experts on this subject based on the ideXlab platform.
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changes in tubulin protein expression in guard cells of Vicia faba l accompanied with dynamic organization of microtubules during the diurnal cycle
Plant and Cell Physiology, 2000Co-Authors: Megumi Fukuda, Seiichiro Hasezawa, Nobuyoshi Nakajima, Noriaki KondoAbstract:We previously reported that the organization of microtubules (MTs) in guard cells of Vicia faba L. shows dynamic diurnal changes [Fukuda et al. (1998) Plant Cell Physiol. 39: 80]. Here, we report a method to directly extract total proteins from guard cells to investigate the biochemical changes in guard cells of Vicia faba L. during the diurnal cycle. Electrophoretic profiles of total proteins of guard cells showed distinct patterns with the time of extraction. Immunoblot analysis also demonstrated changes in a-tubulin and /Mubulin contents with the diurnal cycle. Both tubulins were abundant at 6:00 h and 12:00 h but were almost undetectable at 24:00 h. Although treatment with either actinomycin D or cycloheximide at 18:00 h inhibited neither radial organization of cortical MTs nor stomatal opening, that at 6:00 h inhibited both. These results suggest that the dynamic diurnal changes in the organization of MTs in guard cells and stomatal movement of Vicia faba L. may be, at least partly, regulated by de novo synthesis and decomposition of tubulin molecules in guard cells.
