The Experts below are selected from a list of 81 Experts worldwide ranked by ideXlab platform

Cherupally Krishnan Krishnan Nair - One of the best experts on this subject based on the ideXlab platform.

  • effect of Vinblastine Sulfate on γ radiation induced dna single strand breaks in murine tissues
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2003
    Co-Authors: Rema Rajagopalan, Sanjeev K Ranjan, Cherupally Krishnan Krishnan Nair
    Abstract:

    Abstract The effect of Vinblastine Sulfate on γ-radiation-induced DNA strand breaks in different tissues of tumour bearing mice, was studied by single-cell gel electrophoresis. Intraperitonial administration of different doses (0.25–2.0 mg/kg body weight) of Vinblastine Sulfate 30 min prior to 4 Gy γ-radiation exposure showed a dose-dependent decrease in the yield of DNA strand breaks in murine fibrosarcoma, blood leukocytes and bone marrow cells. The dose-dependent protection of cellular DNA against radiation-induced strand breaks as evidenced from comet tail length, tail moment and percent DNA in the tail, was more pronounced in bone marrow cells than in the cells of the tumor fibrosarcoma. In fibrosarcoma cells, the decrease in comet tail length, tail moment and percent DNA in the tail was detected at lower doses of Vinblastine Sulfate administration and these parameters were not significantly altered at higher doses, from that of the control irradiated. From this study, it appears that in addition to anticancer activity, Vinblastine Sulfate could offer protection to the normal tissues against γ-radiation-induced DNA strand breaks.

Rema Rajagopalan - One of the best experts on this subject based on the ideXlab platform.

  • effect of Vinblastine Sulfate on γ radiation induced dna single strand breaks in murine tissues
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2003
    Co-Authors: Rema Rajagopalan, Sanjeev K Ranjan, Cherupally Krishnan Krishnan Nair
    Abstract:

    Abstract The effect of Vinblastine Sulfate on γ-radiation-induced DNA strand breaks in different tissues of tumour bearing mice, was studied by single-cell gel electrophoresis. Intraperitonial administration of different doses (0.25–2.0 mg/kg body weight) of Vinblastine Sulfate 30 min prior to 4 Gy γ-radiation exposure showed a dose-dependent decrease in the yield of DNA strand breaks in murine fibrosarcoma, blood leukocytes and bone marrow cells. The dose-dependent protection of cellular DNA against radiation-induced strand breaks as evidenced from comet tail length, tail moment and percent DNA in the tail, was more pronounced in bone marrow cells than in the cells of the tumor fibrosarcoma. In fibrosarcoma cells, the decrease in comet tail length, tail moment and percent DNA in the tail was detected at lower doses of Vinblastine Sulfate administration and these parameters were not significantly altered at higher doses, from that of the control irradiated. From this study, it appears that in addition to anticancer activity, Vinblastine Sulfate could offer protection to the normal tissues against γ-radiation-induced DNA strand breaks.

  • Effect of Vinblastine Sulfate on gamma-radiation-induced DNA single-strand breaks in murine tissues.
    Mutation research, 2003
    Co-Authors: Rema Rajagopalan, Sanjeev K Ranjan, Cherupally Krishnan K Nair
    Abstract:

    The effect of Vinblastine Sulfate on gamma-radiation-induced DNA strand breaks in different tissues of tumour bearing mice, was studied by single-cell gel electrophoresis. Intraperitonial administration of different doses (0.25-2.0mg/kg body weight) of Vinblastine Sulfate 30 min prior to 4 Gy gamma-radiation exposure showed a dose-dependent decrease in the yield of DNA strand breaks in murine fibrosarcoma, blood leukocytes and bone marrow cells. The dose-dependent protection of cellular DNA against radiation-induced strand breaks as evidenced from comet tail length, tail moment and percent DNA in the tail, was more pronounced in bone marrow cells than in the cells of the tumor fibrosarcoma. In fibrosarcoma cells, the decrease in comet tail length, tail moment and percent DNA in the tail was detected at lower doses of Vinblastine Sulfate administration and these parameters were not significantly altered at higher doses, from that of the control irradiated. From this study, it appears that in addition to anticancer activity, Vinblastine Sulfate could offer protection to the normal tissues against gamma-radiation-induced DNA strand breaks.

Sanjeev K Ranjan - One of the best experts on this subject based on the ideXlab platform.

  • effect of Vinblastine Sulfate on γ radiation induced dna single strand breaks in murine tissues
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2003
    Co-Authors: Rema Rajagopalan, Sanjeev K Ranjan, Cherupally Krishnan Krishnan Nair
    Abstract:

    Abstract The effect of Vinblastine Sulfate on γ-radiation-induced DNA strand breaks in different tissues of tumour bearing mice, was studied by single-cell gel electrophoresis. Intraperitonial administration of different doses (0.25–2.0 mg/kg body weight) of Vinblastine Sulfate 30 min prior to 4 Gy γ-radiation exposure showed a dose-dependent decrease in the yield of DNA strand breaks in murine fibrosarcoma, blood leukocytes and bone marrow cells. The dose-dependent protection of cellular DNA against radiation-induced strand breaks as evidenced from comet tail length, tail moment and percent DNA in the tail, was more pronounced in bone marrow cells than in the cells of the tumor fibrosarcoma. In fibrosarcoma cells, the decrease in comet tail length, tail moment and percent DNA in the tail was detected at lower doses of Vinblastine Sulfate administration and these parameters were not significantly altered at higher doses, from that of the control irradiated. From this study, it appears that in addition to anticancer activity, Vinblastine Sulfate could offer protection to the normal tissues against γ-radiation-induced DNA strand breaks.

  • Effect of Vinblastine Sulfate on gamma-radiation-induced DNA single-strand breaks in murine tissues.
    Mutation research, 2003
    Co-Authors: Rema Rajagopalan, Sanjeev K Ranjan, Cherupally Krishnan K Nair
    Abstract:

    The effect of Vinblastine Sulfate on gamma-radiation-induced DNA strand breaks in different tissues of tumour bearing mice, was studied by single-cell gel electrophoresis. Intraperitonial administration of different doses (0.25-2.0mg/kg body weight) of Vinblastine Sulfate 30 min prior to 4 Gy gamma-radiation exposure showed a dose-dependent decrease in the yield of DNA strand breaks in murine fibrosarcoma, blood leukocytes and bone marrow cells. The dose-dependent protection of cellular DNA against radiation-induced strand breaks as evidenced from comet tail length, tail moment and percent DNA in the tail, was more pronounced in bone marrow cells than in the cells of the tumor fibrosarcoma. In fibrosarcoma cells, the decrease in comet tail length, tail moment and percent DNA in the tail was detected at lower doses of Vinblastine Sulfate administration and these parameters were not significantly altered at higher doses, from that of the control irradiated. From this study, it appears that in addition to anticancer activity, Vinblastine Sulfate could offer protection to the normal tissues against gamma-radiation-induced DNA strand breaks.

Stephen D Dertinger - One of the best experts on this subject based on the ideXlab platform.

  • in vivo pig a and micronucleus study of the prototypical aneugen Vinblastine Sulfate
    Environmental and Molecular Mutagenesis, 2018
    Co-Authors: Svetlana L Avlasevich, Carson Labash, Dorothea K Torous, Jeffrey C Bemis, James T Macgregor, Stephen D Dertinger
    Abstract:

    The Pig-a assay is being used in regulatory studies to evaluate the potential of agents to induce somatic cell gene mutations and an OECD test guideline is under development. A working group involved with establishing the guideline recently noted that representative aneugenic agents had not been evaluated, and to help fill this data gap Pig-a mutant phenotype and micronucleated reticulocyte frequencies were measured in an integrated study design to assess the mutagenic and cytogenetic damage responses to Vinblastine Sulfate exposure. Male Sprague Dawley rats were treated for twenty-eight consecutive days with Vinblastine dose levels from 0.0156 to 0.125 mg/kg/day. Micronucleated reticulocyte frequencies in peripheral blood were determined at Days 4 and 29, and mutant cell frequencies were determined at Days −4, 15, 29, and 46. Vinblastine affected reticulocyte frequencies, with reductions noted during the treatment phase and increases observed following cessation of treatment. Micronucleated reticulocyte frequencies were significantly elevated at Day 4 in the high dose group. Although a statistically significant increase in mutant reticulocyte frequencies were found for one dose group at a single time point (Day 46), it was not deemed biologically relevant because there was no analogous finding in mutant RBCs, it occurred at the lowest dose tested, and only 1 rat exceeded an upper bound tolerance interval established with historical negative control rats. Therefore, whereas micronucleus induction reflects Vinblastine's well-established aneugenic effect on hematopoietic cells, the lack of a Pig-a response indicates that this tubulin-binding agent does not cause appreciable mutagenicity in this same cell type. Environ. Mol. Mutagen., 2017. © 2017 Wiley Periodicals, Inc.

  • In vivo pig‐a and micronucleus study of the prototypical aneugen Vinblastine Sulfate
    Environmental and Molecular Mutagenesis, 2017
    Co-Authors: Svetlana L Avlasevich, Carson Labash, Dorothea K Torous, Jeffrey C Bemis, James T Macgregor, Stephen D Dertinger
    Abstract:

    The Pig-a assay is being used in regulatory studies to evaluate the potential of agents to induce somatic cell gene mutations and an OECD test guideline is under development. A working group involved with establishing the guideline recently noted that representative aneugenic agents had not been evaluated, and to help fill this data gap Pig-a mutant phenotype and micronucleated reticulocyte frequencies were measured in an integrated study design to assess the mutagenic and cytogenetic damage responses to Vinblastine Sulfate exposure. Male Sprague Dawley rats were treated for twenty-eight consecutive days with Vinblastine dose levels from 0.0156 to 0.125 mg/kg/day. Micronucleated reticulocyte frequencies in peripheral blood were determined at Days 4 and 29, and mutant cell frequencies were determined at Days −4, 15, 29, and 46. Vinblastine affected reticulocyte frequencies, with reductions noted during the treatment phase and increases observed following cessation of treatment. Micronucleated reticulocyte frequencies were significantly elevated at Day 4 in the high dose group. Although a statistically significant increase in mutant reticulocyte frequencies were found for one dose group at a single time point (Day 46), it was not deemed biologically relevant because there was no analogous finding in mutant RBCs, it occurred at the lowest dose tested, and only 1 rat exceeded an upper bound tolerance interval established with historical negative control rats. Therefore, whereas micronucleus induction reflects Vinblastine's well-established aneugenic effect on hematopoietic cells, the lack of a Pig-a response indicates that this tubulin-binding agent does not cause appreciable mutagenicity in this same cell type. Environ. Mol. Mutagen., 2017. © 2017 Wiley Periodicals, Inc.

Svetlana L Avlasevich - One of the best experts on this subject based on the ideXlab platform.

  • in vivo pig a and micronucleus study of the prototypical aneugen Vinblastine Sulfate
    Environmental and Molecular Mutagenesis, 2018
    Co-Authors: Svetlana L Avlasevich, Carson Labash, Dorothea K Torous, Jeffrey C Bemis, James T Macgregor, Stephen D Dertinger
    Abstract:

    The Pig-a assay is being used in regulatory studies to evaluate the potential of agents to induce somatic cell gene mutations and an OECD test guideline is under development. A working group involved with establishing the guideline recently noted that representative aneugenic agents had not been evaluated, and to help fill this data gap Pig-a mutant phenotype and micronucleated reticulocyte frequencies were measured in an integrated study design to assess the mutagenic and cytogenetic damage responses to Vinblastine Sulfate exposure. Male Sprague Dawley rats were treated for twenty-eight consecutive days with Vinblastine dose levels from 0.0156 to 0.125 mg/kg/day. Micronucleated reticulocyte frequencies in peripheral blood were determined at Days 4 and 29, and mutant cell frequencies were determined at Days −4, 15, 29, and 46. Vinblastine affected reticulocyte frequencies, with reductions noted during the treatment phase and increases observed following cessation of treatment. Micronucleated reticulocyte frequencies were significantly elevated at Day 4 in the high dose group. Although a statistically significant increase in mutant reticulocyte frequencies were found for one dose group at a single time point (Day 46), it was not deemed biologically relevant because there was no analogous finding in mutant RBCs, it occurred at the lowest dose tested, and only 1 rat exceeded an upper bound tolerance interval established with historical negative control rats. Therefore, whereas micronucleus induction reflects Vinblastine's well-established aneugenic effect on hematopoietic cells, the lack of a Pig-a response indicates that this tubulin-binding agent does not cause appreciable mutagenicity in this same cell type. Environ. Mol. Mutagen., 2017. © 2017 Wiley Periodicals, Inc.

  • In vivo pig‐a and micronucleus study of the prototypical aneugen Vinblastine Sulfate
    Environmental and Molecular Mutagenesis, 2017
    Co-Authors: Svetlana L Avlasevich, Carson Labash, Dorothea K Torous, Jeffrey C Bemis, James T Macgregor, Stephen D Dertinger
    Abstract:

    The Pig-a assay is being used in regulatory studies to evaluate the potential of agents to induce somatic cell gene mutations and an OECD test guideline is under development. A working group involved with establishing the guideline recently noted that representative aneugenic agents had not been evaluated, and to help fill this data gap Pig-a mutant phenotype and micronucleated reticulocyte frequencies were measured in an integrated study design to assess the mutagenic and cytogenetic damage responses to Vinblastine Sulfate exposure. Male Sprague Dawley rats were treated for twenty-eight consecutive days with Vinblastine dose levels from 0.0156 to 0.125 mg/kg/day. Micronucleated reticulocyte frequencies in peripheral blood were determined at Days 4 and 29, and mutant cell frequencies were determined at Days −4, 15, 29, and 46. Vinblastine affected reticulocyte frequencies, with reductions noted during the treatment phase and increases observed following cessation of treatment. Micronucleated reticulocyte frequencies were significantly elevated at Day 4 in the high dose group. Although a statistically significant increase in mutant reticulocyte frequencies were found for one dose group at a single time point (Day 46), it was not deemed biologically relevant because there was no analogous finding in mutant RBCs, it occurred at the lowest dose tested, and only 1 rat exceeded an upper bound tolerance interval established with historical negative control rats. Therefore, whereas micronucleus induction reflects Vinblastine's well-established aneugenic effect on hematopoietic cells, the lack of a Pig-a response indicates that this tubulin-binding agent does not cause appreciable mutagenicity in this same cell type. Environ. Mol. Mutagen., 2017. © 2017 Wiley Periodicals, Inc.