The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Haiyan Xiang - One of the best experts on this subject based on the ideXlab platform.
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antioxidant profiling of Vine tea ampelopsis grossedentata off line coupling heart cutting hsccc with hplc dad qtof ms ms
Food Chemistry, 2017Co-Authors: Qingping Gao, Lin Chen, Shuyun Shi, Ping Cai, Shuihan Zhang, Haiyan XiangAbstract:Abstract Vine tea with strong antioxidant activity is commonly consumed as healthy tea/beverage. However, detailed information about its antioxidants is incomplete. Here, off-line hyphenation of heart-cutting high-speed countercurrent chromatography (HSCCC) with high performance liquid chromatography–diode array detector–quadrupole time-of-flight tandem mass spectrometry (HPLC–DAD–QTOF-MS/MS) were described for systematic profiling antioxidants in Vine tea. At first, antioxidants were rapidly screened by 1,1-diphenyl-2-picryl-hydrazyl radical–high performance liquid chromatography (DPPH–HPLC). Subsequently, stepwise HSCCC using petroleum ether–ethyl acetate–methanol–water (4:9:4:9, v/v/v/v) and (4:9:5:8, v/v/v/v) as solvent systems was optimized to fractionate and enrich antioxidants from ethyl acetate fraction of Vine tea. Finally, heart-cutting mode was used to collect five interesting HSCCC fractions for HPLC–DAD–QTOF-MS/MS analysis. Desirable orthogonality between HSCCC and HPLC led to identification of fifteen antioxidant flavonoids, while four minor flavonoids were first reported in Vine tea. Results showed that the developed system is efficient to comprehensively explore antioxidants from complex natural herbs.
Marcin Pietrzykowski - One of the best experts on this subject based on the ideXlab platform.
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tree species and soil substrate effects on soil biota during early soil forming stages at afforested mine sites
Applied Soil Ecology, 2016Co-Authors: Agnieszka Jozefowska, Bartlomiej Woś, Marcin PietrzykowskiAbstract:Development of soil microbial and soil fauna communities is a key factor in the soil forming process at reclaimed post-mine sites. The objective of this study was to determine the influence of reclaimed mine soil (RMS) properties (texture, pH, total organic carbon (TOC) and total nitrogen (TN)) and planted tree species on soil biological activity (dehydrogenase activity (DHA), microbial biomass carbon (MBC), enchytraeids (Enchytraeidae) and earthworm (Lumbricidae) communities). Research sites were located in southern Poland on an afforested sand mine pit (Szczakowa) and a sulphur mine spoil heap (Piaseczno). Study plots were arranged in pure tree stands of pine, larch, oak, birch and alder with 25- to 35-year-old trees growing on different RMS substrates: quaternary sands; quaternary loamy sands and loams; neogene clays; and mixed quaternary sands and neogene clay. Microbial biomass carbon depended mainly on the texture, TOC and TN content in the uppermost soil layer, whereas DHA was associated with the fine root annual increment. Earthworm biomass was significantly correlated with clay content, pH and TOC in the uppermost organic soil layers. Among planted tree species, black alder clearly altered soil microbial parameters, biomass and density of earthworms. The strongest effect on DHA was noted under birch trees. Among the studied tree species, deciduous trees (birch, alder and oak) had a greater impact on soil biological properties compared to coniferous trees (pine and larch).
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linking heavy metal bioavailability cd cu zn and pb in scots pine needles to soil properties in reclaimed mine areas
Science of The Total Environment, 2014Co-Authors: Marcin Pietrzykowski, Jaroslaw Socha, Natalie S Van DoornAbstract:article i nfo This work deals with bioaccumulation of Zn, Pb, Cu and Cd in foliage of Scots pine, grown on mine soils. Regression models were used to describe relationships between pine elements bioavailability and biological (dehydrogenase activity) and physico-chemical properties of mine soils developed at different parental rocks. Concentration of trace elements in post-mine ecosystems did not differ from data for Scots pine on natural sites. We conclude that, in this part of Europe in afforested areas affected by hard coal, sand, lignite and sulphur mining, there is no risk of trace element concentrations in mine soils. An exception was in the case of Cd in soils on sand quarry and hard coal spoil heap located in the Upper Silesia region, which was more due to industrial pressure and pollutant deposition than the original Cd concentration in parental rocks.
Qingping Gao - One of the best experts on this subject based on the ideXlab platform.
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antioxidant profiling of Vine tea ampelopsis grossedentata off line coupling heart cutting hsccc with hplc dad qtof ms ms
Food Chemistry, 2017Co-Authors: Qingping Gao, Lin Chen, Shuyun Shi, Ping Cai, Shuihan Zhang, Haiyan XiangAbstract:Abstract Vine tea with strong antioxidant activity is commonly consumed as healthy tea/beverage. However, detailed information about its antioxidants is incomplete. Here, off-line hyphenation of heart-cutting high-speed countercurrent chromatography (HSCCC) with high performance liquid chromatography–diode array detector–quadrupole time-of-flight tandem mass spectrometry (HPLC–DAD–QTOF-MS/MS) were described for systematic profiling antioxidants in Vine tea. At first, antioxidants were rapidly screened by 1,1-diphenyl-2-picryl-hydrazyl radical–high performance liquid chromatography (DPPH–HPLC). Subsequently, stepwise HSCCC using petroleum ether–ethyl acetate–methanol–water (4:9:4:9, v/v/v/v) and (4:9:5:8, v/v/v/v) as solvent systems was optimized to fractionate and enrich antioxidants from ethyl acetate fraction of Vine tea. Finally, heart-cutting mode was used to collect five interesting HSCCC fractions for HPLC–DAD–QTOF-MS/MS analysis. Desirable orthogonality between HSCCC and HPLC led to identification of fifteen antioxidant flavonoids, while four minor flavonoids were first reported in Vine tea. Results showed that the developed system is efficient to comprehensively explore antioxidants from complex natural herbs.
Ping Cai - One of the best experts on this subject based on the ideXlab platform.
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antioxidant profiling of Vine tea ampelopsis grossedentata off line coupling heart cutting hsccc with hplc dad qtof ms ms
Food Chemistry, 2017Co-Authors: Qingping Gao, Lin Chen, Shuyun Shi, Ping Cai, Shuihan Zhang, Haiyan XiangAbstract:Abstract Vine tea with strong antioxidant activity is commonly consumed as healthy tea/beverage. However, detailed information about its antioxidants is incomplete. Here, off-line hyphenation of heart-cutting high-speed countercurrent chromatography (HSCCC) with high performance liquid chromatography–diode array detector–quadrupole time-of-flight tandem mass spectrometry (HPLC–DAD–QTOF-MS/MS) were described for systematic profiling antioxidants in Vine tea. At first, antioxidants were rapidly screened by 1,1-diphenyl-2-picryl-hydrazyl radical–high performance liquid chromatography (DPPH–HPLC). Subsequently, stepwise HSCCC using petroleum ether–ethyl acetate–methanol–water (4:9:4:9, v/v/v/v) and (4:9:5:8, v/v/v/v) as solvent systems was optimized to fractionate and enrich antioxidants from ethyl acetate fraction of Vine tea. Finally, heart-cutting mode was used to collect five interesting HSCCC fractions for HPLC–DAD–QTOF-MS/MS analysis. Desirable orthogonality between HSCCC and HPLC led to identification of fifteen antioxidant flavonoids, while four minor flavonoids were first reported in Vine tea. Results showed that the developed system is efficient to comprehensively explore antioxidants from complex natural herbs.
Shuihan Zhang - One of the best experts on this subject based on the ideXlab platform.
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antioxidant profiling of Vine tea ampelopsis grossedentata off line coupling heart cutting hsccc with hplc dad qtof ms ms
Food Chemistry, 2017Co-Authors: Qingping Gao, Lin Chen, Shuyun Shi, Ping Cai, Shuihan Zhang, Haiyan XiangAbstract:Abstract Vine tea with strong antioxidant activity is commonly consumed as healthy tea/beverage. However, detailed information about its antioxidants is incomplete. Here, off-line hyphenation of heart-cutting high-speed countercurrent chromatography (HSCCC) with high performance liquid chromatography–diode array detector–quadrupole time-of-flight tandem mass spectrometry (HPLC–DAD–QTOF-MS/MS) were described for systematic profiling antioxidants in Vine tea. At first, antioxidants were rapidly screened by 1,1-diphenyl-2-picryl-hydrazyl radical–high performance liquid chromatography (DPPH–HPLC). Subsequently, stepwise HSCCC using petroleum ether–ethyl acetate–methanol–water (4:9:4:9, v/v/v/v) and (4:9:5:8, v/v/v/v) as solvent systems was optimized to fractionate and enrich antioxidants from ethyl acetate fraction of Vine tea. Finally, heart-cutting mode was used to collect five interesting HSCCC fractions for HPLC–DAD–QTOF-MS/MS analysis. Desirable orthogonality between HSCCC and HPLC led to identification of fifteen antioxidant flavonoids, while four minor flavonoids were first reported in Vine tea. Results showed that the developed system is efficient to comprehensively explore antioxidants from complex natural herbs.
