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Li-kuang Chen - One of the best experts on this subject based on the ideXlab platform.
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Aspirin plus tirofiban inhibit the thrombosis induced by Russell's Viper Venom.
Thrombosis Journal, 2016Co-Authors: Ping-tse Chou, Li-kuang ChenAbstract:Background Thrombosis and coagulopathy are the commonest hematological manifestations of enVenomation of Russell’s Viper Venom (RVV). Factor X is activated by a factor X-activating enzyme from Russell’s Viper Venom (RVV-X) to start the coagulation cascade. We established an animal model with local ischemic effects induced by RVV. We tried to treat RVV enVenomation with antiplatelets and anticoagulants without recourse to antiVenom.
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Aspirin plus tirofiban inhibit the thrombosis induced by Russell’s Viper Venom
Thrombosis Journal, 2016Co-Authors: Ren-chieh Wu, Ping-tse Chou, Li-kuang ChenAbstract:Background Thrombosis and coagulopathy are the commonest hematological manifestations of enVenomation of Russell’s Viper Venom (RVV). Factor X is activated by a factor X-activating enzyme from Russell’s Viper Venom (RVV-X) to start the coagulation cascade. We established an animal model with local ischemic effects induced by RVV. We tried to treat RVV enVenomation with antiplatelets and anticoagulants without recourse to antiVenom. Methods RVV was injected into the foot pad of mice. We observed the effects at different intervals and compared local changes in ischemia with drug treatment after 30 min. Results A combination of aspirin plus tirofiban could prevent the ischemic change induced by RVV. The antithrombotic effects of single-use of aspirin or tirofiban were better than single-use of heparin or clopidogrel. Conclusion The aspirin + tirofiban group had a better outcome with respect to prevention of tissue ischemia and gangrene. This indicates that the activation and aggregation of platelets is the major cause of thrombosis induced by RVV.
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Aspirin plus tirofiban inhibit the thrombosis induced by Russell's Viper Venom.
Thrombosis journal, 2016Co-Authors: Ping-tse Chou, Li-kuang ChenAbstract:Thrombosis and coagulopathy are the commonest hematological manifestations of enVenomation of Russell's Viper Venom (RVV). Factor X is activated by a factor X-activating enzyme from Russell's Viper Venom (RVV-X) to start the coagulation cascade. We established an animal model with local ischemic effects induced by RVV. We tried to treat RVV enVenomation with antiplatelets and anticoagulants without recourse to antiVenom. RVV was injected into the foot pad of mice. We observed the effects at different intervals and compared local changes in ischemia with drug treatment after 30 min. A combination of aspirin plus tirofiban could prevent the ischemic change induced by RVV. The antithrombotic effects of single-use of aspirin or tirofiban were better than single-use of heparin or clopidogrel. The aspirin + tirofiban group had a better outcome with respect to prevention of tissue ischemia and gangrene. This indicates that the activation and aggregation of platelets is the major cause of thrombosis induced by RVV.
Ping-tse Chou - One of the best experts on this subject based on the ideXlab platform.
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Aspirin plus tirofiban inhibit the thrombosis induced by Russell's Viper Venom.
Thrombosis Journal, 2016Co-Authors: Ping-tse Chou, Li-kuang ChenAbstract:Background Thrombosis and coagulopathy are the commonest hematological manifestations of enVenomation of Russell’s Viper Venom (RVV). Factor X is activated by a factor X-activating enzyme from Russell’s Viper Venom (RVV-X) to start the coagulation cascade. We established an animal model with local ischemic effects induced by RVV. We tried to treat RVV enVenomation with antiplatelets and anticoagulants without recourse to antiVenom.
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Aspirin plus tirofiban inhibit the thrombosis induced by Russell’s Viper Venom
Thrombosis Journal, 2016Co-Authors: Ren-chieh Wu, Ping-tse Chou, Li-kuang ChenAbstract:Background Thrombosis and coagulopathy are the commonest hematological manifestations of enVenomation of Russell’s Viper Venom (RVV). Factor X is activated by a factor X-activating enzyme from Russell’s Viper Venom (RVV-X) to start the coagulation cascade. We established an animal model with local ischemic effects induced by RVV. We tried to treat RVV enVenomation with antiplatelets and anticoagulants without recourse to antiVenom. Methods RVV was injected into the foot pad of mice. We observed the effects at different intervals and compared local changes in ischemia with drug treatment after 30 min. Results A combination of aspirin plus tirofiban could prevent the ischemic change induced by RVV. The antithrombotic effects of single-use of aspirin or tirofiban were better than single-use of heparin or clopidogrel. Conclusion The aspirin + tirofiban group had a better outcome with respect to prevention of tissue ischemia and gangrene. This indicates that the activation and aggregation of platelets is the major cause of thrombosis induced by RVV.
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Aspirin plus tirofiban inhibit the thrombosis induced by Russell's Viper Venom.
Thrombosis journal, 2016Co-Authors: Ping-tse Chou, Li-kuang ChenAbstract:Thrombosis and coagulopathy are the commonest hematological manifestations of enVenomation of Russell's Viper Venom (RVV). Factor X is activated by a factor X-activating enzyme from Russell's Viper Venom (RVV-X) to start the coagulation cascade. We established an animal model with local ischemic effects induced by RVV. We tried to treat RVV enVenomation with antiplatelets and anticoagulants without recourse to antiVenom. RVV was injected into the foot pad of mice. We observed the effects at different intervals and compared local changes in ischemia with drug treatment after 30 min. A combination of aspirin plus tirofiban could prevent the ischemic change induced by RVV. The antithrombotic effects of single-use of aspirin or tirofiban were better than single-use of heparin or clopidogrel. The aspirin + tirofiban group had a better outcome with respect to prevention of tissue ischemia and gangrene. This indicates that the activation and aggregation of platelets is the major cause of thrombosis induced by RVV.
Gentian Denas - One of the best experts on this subject based on the ideXlab platform.
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Lupus Anticoagulant Testing: Diluted Russell Viper Venom Time (dRVVT).
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Vittorio Pengo, E. Bison, Alessandra Banzato, Giacomo Zoppellaro, Seena Padayattil Jose, Gentian DenasAbstract:Diluted Russell Viper Venom Time (dRVVT) has become the most popular test to detect Lupus Anticoagulant (LA). dRVVT is more sensitive than other global tests employed to detect LA and is not affected by inhibitors of factor VIII or IX. The test is most successfully implemented if you observe three steps in its execution: screening, mixing, and confirmatory studies. Interference due to the presence of heparin in tested plasma must be excluded by means of thrombin time (TT). The prior use of Vitamin K Antagonists (VKAs) or Non-vitamin K Oral Anticoagulants (NOACs) must also be evaluated by means of International Normalized Ratio, or specific tests, respectively.
Sudha Ramalingam - One of the best experts on this subject based on the ideXlab platform.
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Studies on pharmacological effects of Russell's Viper and Saw-scaled Viper Venom and its neutralization by chicken egg yolk antibodies.
International immunopharmacology, 2008Co-Authors: S. Meenatchisundaram, G. Parameswari, A. Michael, Sudha RamalingamAbstract:AntiVenom antibodies were raised in 24-week-old white leghorn chickens against hemotoxic Venoms of Russell's Viper and Saw-scaled Viper snakes. Booster injections of increasing concentrations of Venom were given at 14days of time interval to raise the antiVenom level in egg yolk. Antibodies were extracted from immunized chicken egg yolk by Polson et al. (Polson A., Von Wechmar M.B., Van Regenmortel M.H.V. Isolation of viral IgY antibodies from yolks of immunized hens. Immunological Communications 1980; 9:475–493.) and further purified by DEAE cellulose ion exchange column chromatography, which gave pure (180–200kDa) specific antibodies against Venom. High titre of more than 1:10,000 antibodies were detected by ELISA at the 135th day of observation. The lethal toxicity and various pharmacological activities like hemorrhagic activity, phospholipase activity, edema and procoagulant activities of Venom were carried out by both in vivo and in vitro methods. The effectiveness of antiVenom in neutralizing these effects was carried out involving pre-incubation type experiments. The median effective dose (ED50) for Russell's Viper Venom was 0.96mg/2LD50/18g mice and for Saw-scaled Viper Venom it was 1.28mg/2LD50/18g mice. One millilitre of specific antiVenom was effective in neutralizing 0.110mg of Russell's Viper and 0.137mg of Saw-scaled Viper Venoms respectively (PD50). AntiVenom was effective in neutralization assays in a dose dependent manner. The results indicate that antibodies raised in chicken could effectively neutralize the pharmacological effects induced by Venoms and chickens therefore present an alternative and cheaper source of specific antibody generation.
Douglas A. Triplett - One of the best experts on this subject based on the ideXlab platform.
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Use of the dilute Russell Viper Venom time (dRVVT): its importance and pitfalls.
Journal of autoimmunity, 2000Co-Authors: Douglas A. TriplettAbstract:Antiphospholipid antibodies (APA) are now recognized as the most common cause of acquired thrombophilia. These antibodies may lead to thrombosis in both arterial and venous sites. Lupus anticoagulants (LA) are the most significant risk factor among the various APAs. The detection of LAs remains challenging to most laboratories. Multiple screening tests are recommended (e.g. APTT, dilute PT and dRVVT). The dRVVT is one of the most important screening procedures. In many instances, commercially available dRVVT systems include a screening reagent with low PL concentration and a confirmatory product with high PL concentration. There are a number of commercially available dRVVT test systems. These reagents vary in phospholipid origin and concentration as well as source of Russell Viper Venom (RVV). It is imperative for laboratories to be well informed regarding reagent composition and laboratory performance.
