The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform
Dario Compagnone - One of the best experts on this subject based on the ideXlab platform.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
Journal of nanobiotechnology, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Guilia Pezzoni, Mohamed Emara, Dario CompagnoneAbstract:Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
BMC, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Mohamed M. Emara, Guilia Pezzoni, Dario CompagnoneAbstract:Abstract Background Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. Results The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). Conclusions The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas
Mervat E. Hamdy - One of the best experts on this subject based on the ideXlab platform.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
Journal of nanobiotechnology, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Guilia Pezzoni, Mohamed Emara, Dario CompagnoneAbstract:Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
BMC, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Mohamed M. Emara, Guilia Pezzoni, Dario CompagnoneAbstract:Abstract Background Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. Results The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). Conclusions The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas
Guilia Pezzoni - One of the best experts on this subject based on the ideXlab platform.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
Journal of nanobiotechnology, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Guilia Pezzoni, Mohamed Emara, Dario CompagnoneAbstract:Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
BMC, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Mohamed M. Emara, Guilia Pezzoni, Dario CompagnoneAbstract:Abstract Background Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. Results The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). Conclusions The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas
Ayman H. El-deeb - One of the best experts on this subject based on the ideXlab platform.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
Journal of nanobiotechnology, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Guilia Pezzoni, Mohamed Emara, Dario CompagnoneAbstract:Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
BMC, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Mohamed M. Emara, Guilia Pezzoni, Dario CompagnoneAbstract:Abstract Background Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. Results The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). Conclusions The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas
Taher A. Salah - One of the best experts on this subject based on the ideXlab platform.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
Journal of nanobiotechnology, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Guilia Pezzoni, Mohamed Emara, Dario CompagnoneAbstract:Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas.
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Development of gold nanoparticles biosensor for ultrasensitive Diagnosis of foot and mouth disease virus
BMC, 2018Co-Authors: Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-deeb, Mohamed M. Emara, Guilia Pezzoni, Dario CompagnoneAbstract:Abstract Background Nano-PCR is a recent tool that is used in Viral Diseases Diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the Diagnosis of different Diseases including Viral Diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific Diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. Results The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a − 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). Conclusions The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV Diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV Diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas
