The Experts below are selected from a list of 42003 Experts worldwide ranked by ideXlab platform
Barbara Muller - One of the best experts on this subject based on the ideXlab platform.
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double labelled hiv 1 particles for study of Virus Cell Interaction
Virology, 2007Co-Authors: Marko Lampe, John A G Briggs, Thomas Endress, Barbel Glass, Stefan Riegelsberger, Hansgeorg Krausslich, Don C Lamb, Christoph Brauchle, Barbara MullerAbstract:Human immunodeficiency Virus (HIV) delivers its genome to a host Cell through fusion of the viral envelope with a Cellular membrane. While the viral and Cellular proteins involved in entry have been analyzed in detail, the dynamics of Virus-Cell fusion are largely unknown. Single Virus tracing (SVT) provides the unique opportunity to visualize viral particles in real time allowing direct observation of the dynamics of this stochastic process. For this purpose, we developed a double-coloured HIV derivative carrying a green fluorescent label attached to the viral matrix protein combined with a red label fused to the viral Vpr protein designed to distinguish between complete virions and subviral particles lacking MA after membrane fusion. We present here a detailed characterization of this novel tool together with exemplary live Cell imaging studies, demonstrating its suitability for real-time analyses of HIV-Cell Interaction.
Don C Lamb - One of the best experts on this subject based on the ideXlab platform.
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double labelled hiv 1 particles for study of Virus Cell Interaction
Virology, 2007Co-Authors: Marko Lampe, John A G Briggs, Thomas Endress, Barbel Glass, Stefan Riegelsberger, Hansgeorg Krausslich, Don C Lamb, Christoph Brauchle, Barbara MullerAbstract:Human immunodeficiency Virus (HIV) delivers its genome to a host Cell through fusion of the viral envelope with a Cellular membrane. While the viral and Cellular proteins involved in entry have been analyzed in detail, the dynamics of Virus-Cell fusion are largely unknown. Single Virus tracing (SVT) provides the unique opportunity to visualize viral particles in real time allowing direct observation of the dynamics of this stochastic process. For this purpose, we developed a double-coloured HIV derivative carrying a green fluorescent label attached to the viral matrix protein combined with a red label fused to the viral Vpr protein designed to distinguish between complete virions and subviral particles lacking MA after membrane fusion. We present here a detailed characterization of this novel tool together with exemplary live Cell imaging studies, demonstrating its suitability for real-time analyses of HIV-Cell Interaction.
Lawrence S Young - One of the best experts on this subject based on the ideXlab platform.
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in vitro production of stable epstein barr Virus positive epithelial Cell clones which resemble the Virus Cell Interaction observed in nasopharyngeal carcinoma
Virology, 1996Co-Authors: Pauline G Knox, Alan B Rickinson, Lawrence S YoungAbstract:The Interaction of Epstein– Barr Virus (EBV) with epithelial Cells and the consequent role of the Virus in the aetiology of undifferentiated nasopharyngeal carcinoma (NPC) is poorly understood. One important obstacle to work in this area has been the lack of an epithelial Cell culture system in which EBV is stably maintained. Using a previously described approach in which CR2-transfected epithelial Cells (SVK-CR2) are rendered susceptible to transient EBV infection (Li et al., Nature 356, 347, 1992), we now demonstrate that the pattern of EBV latent gene transcription in these acutely infected epithelial Cells differs from that observed in Virus-infected primary B Cells. In addition, some of these epithelial Cells spontaneously entered the EBV lytic cycle. By cloning Akata Virus-infected SVK-CR2 Cells we generated two stable lines which remained EBV positive for more than 1.5 years at which time further subclones were isolated. These cloned lines carry the EBV genome as an episome and exclusively use the FQp promoter for driving EBNA1 transcription, display no Cp/Wp promoter activity, and express low levels of the LMP mRNAs. Unlike acutely infected SVK-CR2 Cells, the cloned lines responded poorly to suspension-induced terminal differentiation and were impaired in their ability to enter the Virus lytic cycle. These results, showing similarities between the cloned EBV-positive SVK-CR2 lines and NPC tumour Cells, suggest that stable maintenance of EBV in epithelial Cells may require an undifferentiated Cellular environment. q 1996 Academic Press, Inc.
Marko Lampe - One of the best experts on this subject based on the ideXlab platform.
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double labelled hiv 1 particles for study of Virus Cell Interaction
Virology, 2007Co-Authors: Marko Lampe, John A G Briggs, Thomas Endress, Barbel Glass, Stefan Riegelsberger, Hansgeorg Krausslich, Don C Lamb, Christoph Brauchle, Barbara MullerAbstract:Human immunodeficiency Virus (HIV) delivers its genome to a host Cell through fusion of the viral envelope with a Cellular membrane. While the viral and Cellular proteins involved in entry have been analyzed in detail, the dynamics of Virus-Cell fusion are largely unknown. Single Virus tracing (SVT) provides the unique opportunity to visualize viral particles in real time allowing direct observation of the dynamics of this stochastic process. For this purpose, we developed a double-coloured HIV derivative carrying a green fluorescent label attached to the viral matrix protein combined with a red label fused to the viral Vpr protein designed to distinguish between complete virions and subviral particles lacking MA after membrane fusion. We present here a detailed characterization of this novel tool together with exemplary live Cell imaging studies, demonstrating its suitability for real-time analyses of HIV-Cell Interaction.
David R Katz - One of the best experts on this subject based on the ideXlab platform.
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herpes simplex Virus type 1 induced activation of myeloid dendritic Cells the roles of Virus Cell Interaction and paracrine type i ifn secretion
Journal of Immunology, 2004Co-Authors: Gabriele Pollara, Meleri Jones, Matthew E Handley, Mansi Rajpopat, Antonia Kwan, Robert S Coffin, Graham R Foster, Benjamin M Chain, David R KatzAbstract:Adaptive Cellular immunity is required to clear HSV-1 infection in the periphery. Myeloid dendritic Cells (DCs) are the first professional Ag-presenting Cell to encounter the Virus after primary and secondary infection and thus the consequences of their infection are important in understanding the pathogenesis of the disease and the response to the Virus. Following HSV-1 infection, both uninfected and infected human DCs acquire a more mature phenotype. In this study, we demonstrate that type I IFN secreted from myeloid DC mediates bystander activation of the uninfected DCs. Furthermore, we confirm that this IFN primes DCs for elevated IL-12 p40 and p70 secretion. However, secretion of IFN is not responsible for the acquisition of a mature phenotype by HSV-1-infected DC. Rather, Virus binding to a receptor on the Cell surface induces DC maturation directly, through activation of the NF-kappaB and p38 MAPK pathways. The binding of HSV glycoprotein D is critical to the acquisition of a mature phenotype and type I IFN secretion. The data therefore demonstrate that DCs can respond to HSV exposure directly through recognition of viral envelope structures. In the context of natural HSV infection, the coupling of viral entry to the activation of DC signaling pathways is likely to be counterbalanced by viral disruption of DC maturation. However, the parallel release of type I IFN may result in paracrine activation so that the DCs are nonetheless able to mount an adaptive immune response.
