The Experts below are selected from a list of 39786 Experts worldwide ranked by ideXlab platform
Jens H Kuhn - One of the best experts on this subject based on the ideXlab platform.
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Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses
Emerging Infectious Diseases, 2016Co-Authors: Andy Kilianski, Pierce A. Roth, Andrew Tanny Liem, Jessica M. Hill, Kristen L. Willis, Rebecca D. Rossmaier, Andrew V. Marinich, Mark Karavis, Michele N Maughan, Jens H KuhnAbstract:Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of Virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis Virus and Ebola Virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA Virus Characterization.
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Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses.
Emerging infectious diseases, 2016Co-Authors: Andy Kilianski, Pierce A. Roth, Andrew Tanny Liem, Jessica M. Hill, Kristen L. Willis, Rebecca D. Rossmaier, Andrew V. Marinich, Mark Karavis, Michele N Maughan, Jens H KuhnAbstract:Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of Virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis Virus and Ebola Virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA Virus Characterization.
Andy Kilianski - One of the best experts on this subject based on the ideXlab platform.
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Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses
Emerging Infectious Diseases, 2016Co-Authors: Andy Kilianski, Pierce A. Roth, Andrew Tanny Liem, Jessica M. Hill, Kristen L. Willis, Rebecca D. Rossmaier, Andrew V. Marinich, Mark Karavis, Michele N Maughan, Jens H KuhnAbstract:Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of Virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis Virus and Ebola Virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA Virus Characterization.
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Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses.
Emerging infectious diseases, 2016Co-Authors: Andy Kilianski, Pierce A. Roth, Andrew Tanny Liem, Jessica M. Hill, Kristen L. Willis, Rebecca D. Rossmaier, Andrew V. Marinich, Mark Karavis, Michele N Maughan, Jens H KuhnAbstract:Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of Virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis Virus and Ebola Virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA Virus Characterization.
Wayne L. Gray - One of the best experts on this subject based on the ideXlab platform.
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Simian varicella Virus: Characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster Virus.
Journal of General Virology, 1992Co-Authors: Thomas M. Fletcher, Wayne L. GrayAbstract:Simian varicella Virus (SVV) causes a varicella-like disease in non-human primates. In this study, SVV virions were purified from SVV-infected BSC-1 cells by zonal and differential gradient centrifugation and the virion polypeptide composition was analysed by SDS-PAGE. SVV virions had a buoyant density of 1.21 g/ml, identical to the value obtained for varicella-zoster Virus (VZV) virions purified by the same method. Electron microscopy of the concentrated SVV virions revealed characteristic herpesVirus morphology. SVV virions consisted of at least 30 polypeptide species ranging from 16K to >200K. The electrophoretic profiles of radiolabelled SVV and VZV virion polypeptides were very similar. Immunoprecipitations of solubilized SVV-infected cell preparations using SVV immune sera revealed at least 18 viral polypeptides with an M r range of 12K to 142K and six glycoproteins ranging from 46K to 115K. In addition, extensive cross-reactivity between SVV and VZV proteins and glycoproteins was demonstrated by immunoprecipitation with heterologous immune sera. The high degree of antigenic relatedness between SVV and VZV provides further support for simian varicella as a model for VZV infections.
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Simian varicella Virus: Characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster Virus.
The Journal of general virology, 1992Co-Authors: Thomas M. Fletcher, Wayne L. GrayAbstract:Simian varicella Virus (SVV) causes a varicella-like disease in non-human primates. In this study, SVV virions were purified from SVV-infected BSC-1 cells by zonal and differential gradient centrifugation and the virion polypeptide composition was analysed by SDS-PAGE. SVV virions had a buoyant density of 1.21 g/ml, identical to the value obtained for varicella-zoster Virus (VZV) virions purified by the same method. Electron microscopy of the concentrated SVV virions revealed characteristic herpesVirus morphology. SVV virions consisted of at least 30 polypeptide species ranging from 16K to greater than 200K. The electrophoretic profiles of radiolabelled SVV and VZV virion polypeptides were very similar. Immunoprecipitations of solubilized SVV-infected cell preparations using SVV immune sera revealed at least 18 viral polypeptides with an Mr range of 12K to 142K and six glycoproteins ranging from 46K to 115K. In addition, extensive cross-reactivity between SVV and VZV proteins and glycoproteins was demonstrated by immunoprecipitation with heterologous immune sera. The high degree of antigenic relatedness between SVV and VZV provides further support for simian varicella as a model for VZV infections.
Claire Geslin - One of the best experts on this subject based on the ideXlab platform.
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living side by side with a Virus Characterization of two novel plasmids from thermococcus prieurii a host for the spindle shaped Virus tpv1
Applied and Environmental Microbiology, 2013Co-Authors: Aurore Gorlas, Patrick Forterre, Mart Krupovic, Claire GeslinAbstract:Microbial cells often serve as an evolutionary battlefield for different types of mobile genetic elements, such as Viruses and plasmids. Here, we describe the isolation and Characterization of two new archaeal plasmids which share the host with the spindle-shaped Thermococcus prieurii Virus 1 (TPV1). The two plasmids, pTP1 and pTP2, were isolated from the hyperthermophilic archaeon Thermococcus prieurii (phylum Euryarchaeota), a resident of a deep-sea hydrothermal vent located at the East Pacific Rise at 2,700-m depth (7°25′24 S, 107°47′66 W). pTP1 (3.1 kb) and pTP2 (2.0 kb) are among the smallest known plasmids of hyperthermophilic archaea, and both are predicted to replicate via the rolling-circle mechanism. The two plasmids and the Virus TPV1 do not have a single gene in common and stably propagate in infected cells without any apparent antagonistic effect on each other. The compatibility of the three genetic elements and the high copy number of pTP1 and pTP2 plasmids (50 copies/cell) might be useful for developing new genetic tools for studying hyperthermophilic euryarchaea and their Viruses.
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living side by side with a Virus Characterization of two novel plasmids from thermococcus prieurii a host for the spindle shaped Virus tpv1
Applied and Environmental Microbiology, 2013Co-Authors: Aurore Gorlas, Patrick Forterre, Mart Krupovic, Claire GeslinAbstract:Microbial cells often serve as an evolutionary battlefield for different types of mobile genetic elements, such as Viruses and plasmids. Here, we describe the isolation and Characterization of two new archaeal plasmids which share the host with the spindle-shaped Thermococcus prieurii Virus 1 (TPV1). The two plasmids, pTP1 and pTP2, were isolated from the hyperthermophilic archaeon Thermococcus prieurii (phylum Euryarchaeota), a resident of a deep-sea hydrothermal vent located at the East Pacific Rise at 2,700-m depth (7°25′24 S, 107°47′66 W). pTP1 (3.1 kb) and pTP2 (2.0 kb) are among the smallest known plasmids of hyperthermophilic archaea, and both are predicted to replicate via the rolling-circle mechanism. The two plasmids and the Virus TPV1 do not have a single gene in common and stably propagate in infected cells without any apparent antagonistic effect on each other. The compatibility of the three genetic elements and the high copy number of pTP1 and pTP2 plasmids (50 copies/cell) might be useful for developing new genetic tools for studying hyperthermophilic euryarchaea and their Viruses.
Patrick Forterre - One of the best experts on this subject based on the ideXlab platform.
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living side by side with a Virus Characterization of two novel plasmids from thermococcus prieurii a host for the spindle shaped Virus tpv1
Applied and Environmental Microbiology, 2013Co-Authors: Aurore Gorlas, Patrick Forterre, Mart Krupovic, Claire GeslinAbstract:Microbial cells often serve as an evolutionary battlefield for different types of mobile genetic elements, such as Viruses and plasmids. Here, we describe the isolation and Characterization of two new archaeal plasmids which share the host with the spindle-shaped Thermococcus prieurii Virus 1 (TPV1). The two plasmids, pTP1 and pTP2, were isolated from the hyperthermophilic archaeon Thermococcus prieurii (phylum Euryarchaeota), a resident of a deep-sea hydrothermal vent located at the East Pacific Rise at 2,700-m depth (7°25′24 S, 107°47′66 W). pTP1 (3.1 kb) and pTP2 (2.0 kb) are among the smallest known plasmids of hyperthermophilic archaea, and both are predicted to replicate via the rolling-circle mechanism. The two plasmids and the Virus TPV1 do not have a single gene in common and stably propagate in infected cells without any apparent antagonistic effect on each other. The compatibility of the three genetic elements and the high copy number of pTP1 and pTP2 plasmids (50 copies/cell) might be useful for developing new genetic tools for studying hyperthermophilic euryarchaea and their Viruses.
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living side by side with a Virus Characterization of two novel plasmids from thermococcus prieurii a host for the spindle shaped Virus tpv1
Applied and Environmental Microbiology, 2013Co-Authors: Aurore Gorlas, Patrick Forterre, Mart Krupovic, Claire GeslinAbstract:Microbial cells often serve as an evolutionary battlefield for different types of mobile genetic elements, such as Viruses and plasmids. Here, we describe the isolation and Characterization of two new archaeal plasmids which share the host with the spindle-shaped Thermococcus prieurii Virus 1 (TPV1). The two plasmids, pTP1 and pTP2, were isolated from the hyperthermophilic archaeon Thermococcus prieurii (phylum Euryarchaeota), a resident of a deep-sea hydrothermal vent located at the East Pacific Rise at 2,700-m depth (7°25′24 S, 107°47′66 W). pTP1 (3.1 kb) and pTP2 (2.0 kb) are among the smallest known plasmids of hyperthermophilic archaea, and both are predicted to replicate via the rolling-circle mechanism. The two plasmids and the Virus TPV1 do not have a single gene in common and stably propagate in infected cells without any apparent antagonistic effect on each other. The compatibility of the three genetic elements and the high copy number of pTP1 and pTP2 plasmids (50 copies/cell) might be useful for developing new genetic tools for studying hyperthermophilic euryarchaea and their Viruses.
