The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform
A Kijlstra - One of the best experts on this subject based on the ideXlab platform.
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longitudinal analysis of varicella zoster Virus DNA on the ocular surface associated with herpes zoster ophthalmicus
American Journal of Ophthalmology, 2001Co-Authors: Michel J W Zaal, Hennie J Volkerdieben, M Wienesen, J Damaro, A KijlstraAbstract:Abstract PURPOSE: Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface of patients with herpes zoster ophthalmicus. METHODS: Clinical specimens were obtained from the bulbar conjunctival surface with a cotton-tipped swab at weekly intervals for 6 consecutive weeks from 21 patients with acute ophthalmic zoster with a skin rash duration of less than 7 days. All patients received oral valacyclovir 1000 mg three times daily for 10 days without additional corticosteroids. The swabs were analyzed by means of polymerase chain reaction for the presence of varicella-zoster Virus and herpes simplex Virus type 1 DNA. Conjunctival swabs were also obtained from a control group of 20 patients with cataract. RESULTS: On inclusion, varicella-zoster Virus DNA was present on the ocular surface of 19 of the 21 patients. Six varicella-zoster Virus DNA–positive patients had no signs of ocular inflammation. All control swabs were negative for both varicella-zoster Virus and herpes simplex Virus DNA. The duration of varicella-zoster Virus DNA detection from rash onset varied from 2 to 34 days. The number of days between the onset of herpes zoster skin rash and the latest positive varicella-zoster Virus DNA test was significantly longer in patients whose age was equal to or above the median age of 66 years than in the younger patients (Mann-Whitney test: P = .0004). At 6-week follow-up, all conjunctival swabs were negative for varicella-zoster Virus DNA. However, at that time, the eyes of seven patients were still inflamed. CONCLUSION: The duration of varicella-zoster Virus DNA shedding in herpes zoster ophthalmicus is highly variable and age dependent, and is probably related to the host immune response.
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Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface associated with herpes zoster ophthalmicus
American journal of ophthalmology, 2001Co-Authors: Michel J W Zaal, M Wienesen, Hennie J. Völker-dieben, J. D'amaro, A KijlstraAbstract:Abstract PURPOSE: Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface of patients with herpes zoster ophthalmicus. METHODS: Clinical specimens were obtained from the bulbar conjunctival surface with a cotton-tipped swab at weekly intervals for 6 consecutive weeks from 21 patients with acute ophthalmic zoster with a skin rash duration of less than 7 days. All patients received oral valacyclovir 1000 mg three times daily for 10 days without additional corticosteroids. The swabs were analyzed by means of polymerase chain reaction for the presence of varicella-zoster Virus and herpes simplex Virus type 1 DNA. Conjunctival swabs were also obtained from a control group of 20 patients with cataract. RESULTS: On inclusion, varicella-zoster Virus DNA was present on the ocular surface of 19 of the 21 patients. Six varicella-zoster Virus DNA–positive patients had no signs of ocular inflammation. All control swabs were negative for both varicella-zoster Virus and herpes simplex Virus DNA. The duration of varicella-zoster Virus DNA detection from rash onset varied from 2 to 34 days. The number of days between the onset of herpes zoster skin rash and the latest positive varicella-zoster Virus DNA test was significantly longer in patients whose age was equal to or above the median age of 66 years than in the younger patients (Mann-Whitney test: P = .0004). At 6-week follow-up, all conjunctival swabs were negative for varicella-zoster Virus DNA. However, at that time, the eyes of seven patients were still inflamed. CONCLUSION: The duration of varicella-zoster Virus DNA shedding in herpes zoster ophthalmicus is highly variable and age dependent, and is probably related to the host immune response.
José Salas - One of the best experts on this subject based on the ideXlab platform.
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Replication of African swine fever Virus DNA in infected cells.
Virology, 1999Co-Authors: Gema Rojo, Eladio Viñuela, Regina Garcı́a-beato, María L. Salas, José SalasAbstract:We have examined the ultrastructural localization of African swine fever Virus DNA in thin-sections of infected cells by in situ hybridization and autoradiography. Virus-specific DNA sequences were found in the nucleus of infected Vero cells at early times in the synthesis of the viral DNA, forming dense foci localized in proximity to the nuclear membrane. At later times, the viral DNA was found exclusively in the cytoplasm. Electron microscopic autoradiography of African swine fever Virus-infected macrophages showed that the nucleus is also a site of viral DNA replication at early times. These results provide further evidence of the existence of nuclear and cytoplasmic stages in the synthesis of African swine fever Virus DNA. On the other hand, alkaline sucrose sedimentation analysis of the replicative intermediates synthesized in the nucleus and cytoplasm of infected macrophages showed that small DNA fragments ( approximately 6-12S) were synthesized in the nucleus at an early time, whereas at later times, larger fragments of approximately 37-49S were labeled in the cytoplasm. Pulse-chase experiments demonstrated that these fragments are precursors of the mature cross-linked viral DNA. The formation of dimeric concatemers, which are predominantly head-to-head linked, was observed by pulsed-field electrophoresis and restriction enzyme analysis at intermediate and late times in the replication of African swine fever Virus DNA. Our findings suggest that the replication of African swine fever Virus DNA proceeds by a de novo start mechanism with the synthesis of small DNA fragments, which are then converted into larger size molecules. Ligation or further elongation of these molecules would originate a two-unit concatemer with dimeric ends that could be resolved to generate the genomic DNA by site-specific nicking, rearrangement, and ligation as has been proposed in the de novo start model of Baroudy et al. (B. M. Baroudy, S. Venkatesam, and B. Moss, 1982, Cold Spring Harbor Symp. Quant. Biol. 47, 723-729) for the replication of vaccinia Virus DNA.
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Replication of African swine fever Virus DNA in infected cells.
Virology, 1999Co-Authors: Gema Rojo, Eladio Viñuela, Regina Garcı́a-beato, Maria Salas, José SalasAbstract:Abstract We have examined the ultrastructural localization of African swine fever Virus DNA in thin-sections of infected cells byin situhybridization and autoradiography. Virus-specific DNA sequences were found in the nucleus of infected Vero cells at early times in the synthesis of the viral DNA, forming dense foci localized in proximity to the nuclear membrane. At later times, the viral DNA was found exclusively in the cytoplasm. Electron microscopic autoradiography of African swine fever Virus-infected macrophages showed that the nucleus is also a site of viral DNA replication at early times. These results provide further evidence of the existence of nuclear and cytoplasmic stages in the synthesis of African swine fever Virus DNA. On the other hand, alkaline sucrose sedimentation analysis of the replicative intermediates synthesized in the nucleus and cytoplasm of infected macrophages showed that small DNA fragments (∼6–12S) were synthesized in the nucleus at an early time, whereas at later times, larger fragments of ∼37–49S were labeled in the cytoplasm. Pulse–chase experiments demonstrated that these fragments are precursors of the mature cross-linked viral DNA. The formation of dimeric concatemers, which are predominantly head-to-head linked, was observed by pulsed-field electrophoresis and restriction enzyme analysis at intermediate and late times in the replication of African swine fever Virus DNA. Our findings suggest that the replication of African swine fever Virus DNA proceeds by ade novostart mechanism with the synthesis of small DNA fragments, which are then converted into larger size molecules. Ligation or further elongation of these molecules would originate a two-unit concatemer with dimeric ends that could be resolved to generate the genomic DNA by site-specific nicking, rearrangement, and ligation as has been proposed in thede novostart model of Baroudyet al.(B. M. Baroudy, S. Venkatesam, and B. Moss, 1982,Cold Spring Harbor Symp. Quant. Biol.47, 723–729) for the replication of vaccinia Virus DNA.
Michel J W Zaal - One of the best experts on this subject based on the ideXlab platform.
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longitudinal analysis of varicella zoster Virus DNA on the ocular surface associated with herpes zoster ophthalmicus
American Journal of Ophthalmology, 2001Co-Authors: Michel J W Zaal, Hennie J Volkerdieben, M Wienesen, J Damaro, A KijlstraAbstract:Abstract PURPOSE: Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface of patients with herpes zoster ophthalmicus. METHODS: Clinical specimens were obtained from the bulbar conjunctival surface with a cotton-tipped swab at weekly intervals for 6 consecutive weeks from 21 patients with acute ophthalmic zoster with a skin rash duration of less than 7 days. All patients received oral valacyclovir 1000 mg three times daily for 10 days without additional corticosteroids. The swabs were analyzed by means of polymerase chain reaction for the presence of varicella-zoster Virus and herpes simplex Virus type 1 DNA. Conjunctival swabs were also obtained from a control group of 20 patients with cataract. RESULTS: On inclusion, varicella-zoster Virus DNA was present on the ocular surface of 19 of the 21 patients. Six varicella-zoster Virus DNA–positive patients had no signs of ocular inflammation. All control swabs were negative for both varicella-zoster Virus and herpes simplex Virus DNA. The duration of varicella-zoster Virus DNA detection from rash onset varied from 2 to 34 days. The number of days between the onset of herpes zoster skin rash and the latest positive varicella-zoster Virus DNA test was significantly longer in patients whose age was equal to or above the median age of 66 years than in the younger patients (Mann-Whitney test: P = .0004). At 6-week follow-up, all conjunctival swabs were negative for varicella-zoster Virus DNA. However, at that time, the eyes of seven patients were still inflamed. CONCLUSION: The duration of varicella-zoster Virus DNA shedding in herpes zoster ophthalmicus is highly variable and age dependent, and is probably related to the host immune response.
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Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface associated with herpes zoster ophthalmicus
American journal of ophthalmology, 2001Co-Authors: Michel J W Zaal, M Wienesen, Hennie J. Völker-dieben, J. D'amaro, A KijlstraAbstract:Abstract PURPOSE: Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface of patients with herpes zoster ophthalmicus. METHODS: Clinical specimens were obtained from the bulbar conjunctival surface with a cotton-tipped swab at weekly intervals for 6 consecutive weeks from 21 patients with acute ophthalmic zoster with a skin rash duration of less than 7 days. All patients received oral valacyclovir 1000 mg three times daily for 10 days without additional corticosteroids. The swabs were analyzed by means of polymerase chain reaction for the presence of varicella-zoster Virus and herpes simplex Virus type 1 DNA. Conjunctival swabs were also obtained from a control group of 20 patients with cataract. RESULTS: On inclusion, varicella-zoster Virus DNA was present on the ocular surface of 19 of the 21 patients. Six varicella-zoster Virus DNA–positive patients had no signs of ocular inflammation. All control swabs were negative for both varicella-zoster Virus and herpes simplex Virus DNA. The duration of varicella-zoster Virus DNA detection from rash onset varied from 2 to 34 days. The number of days between the onset of herpes zoster skin rash and the latest positive varicella-zoster Virus DNA test was significantly longer in patients whose age was equal to or above the median age of 66 years than in the younger patients (Mann-Whitney test: P = .0004). At 6-week follow-up, all conjunctival swabs were negative for varicella-zoster Virus DNA. However, at that time, the eyes of seven patients were still inflamed. CONCLUSION: The duration of varicella-zoster Virus DNA shedding in herpes zoster ophthalmicus is highly variable and age dependent, and is probably related to the host immune response.
Gema Rojo - One of the best experts on this subject based on the ideXlab platform.
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Replication of African swine fever Virus DNA in infected cells.
Virology, 1999Co-Authors: Gema Rojo, Eladio Viñuela, Regina Garcı́a-beato, María L. Salas, José SalasAbstract:We have examined the ultrastructural localization of African swine fever Virus DNA in thin-sections of infected cells by in situ hybridization and autoradiography. Virus-specific DNA sequences were found in the nucleus of infected Vero cells at early times in the synthesis of the viral DNA, forming dense foci localized in proximity to the nuclear membrane. At later times, the viral DNA was found exclusively in the cytoplasm. Electron microscopic autoradiography of African swine fever Virus-infected macrophages showed that the nucleus is also a site of viral DNA replication at early times. These results provide further evidence of the existence of nuclear and cytoplasmic stages in the synthesis of African swine fever Virus DNA. On the other hand, alkaline sucrose sedimentation analysis of the replicative intermediates synthesized in the nucleus and cytoplasm of infected macrophages showed that small DNA fragments ( approximately 6-12S) were synthesized in the nucleus at an early time, whereas at later times, larger fragments of approximately 37-49S were labeled in the cytoplasm. Pulse-chase experiments demonstrated that these fragments are precursors of the mature cross-linked viral DNA. The formation of dimeric concatemers, which are predominantly head-to-head linked, was observed by pulsed-field electrophoresis and restriction enzyme analysis at intermediate and late times in the replication of African swine fever Virus DNA. Our findings suggest that the replication of African swine fever Virus DNA proceeds by a de novo start mechanism with the synthesis of small DNA fragments, which are then converted into larger size molecules. Ligation or further elongation of these molecules would originate a two-unit concatemer with dimeric ends that could be resolved to generate the genomic DNA by site-specific nicking, rearrangement, and ligation as has been proposed in the de novo start model of Baroudy et al. (B. M. Baroudy, S. Venkatesam, and B. Moss, 1982, Cold Spring Harbor Symp. Quant. Biol. 47, 723-729) for the replication of vaccinia Virus DNA.
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Replication of African swine fever Virus DNA in infected cells.
Virology, 1999Co-Authors: Gema Rojo, Eladio Viñuela, Regina Garcı́a-beato, Maria Salas, José SalasAbstract:Abstract We have examined the ultrastructural localization of African swine fever Virus DNA in thin-sections of infected cells byin situhybridization and autoradiography. Virus-specific DNA sequences were found in the nucleus of infected Vero cells at early times in the synthesis of the viral DNA, forming dense foci localized in proximity to the nuclear membrane. At later times, the viral DNA was found exclusively in the cytoplasm. Electron microscopic autoradiography of African swine fever Virus-infected macrophages showed that the nucleus is also a site of viral DNA replication at early times. These results provide further evidence of the existence of nuclear and cytoplasmic stages in the synthesis of African swine fever Virus DNA. On the other hand, alkaline sucrose sedimentation analysis of the replicative intermediates synthesized in the nucleus and cytoplasm of infected macrophages showed that small DNA fragments (∼6–12S) were synthesized in the nucleus at an early time, whereas at later times, larger fragments of ∼37–49S were labeled in the cytoplasm. Pulse–chase experiments demonstrated that these fragments are precursors of the mature cross-linked viral DNA. The formation of dimeric concatemers, which are predominantly head-to-head linked, was observed by pulsed-field electrophoresis and restriction enzyme analysis at intermediate and late times in the replication of African swine fever Virus DNA. Our findings suggest that the replication of African swine fever Virus DNA proceeds by ade novostart mechanism with the synthesis of small DNA fragments, which are then converted into larger size molecules. Ligation or further elongation of these molecules would originate a two-unit concatemer with dimeric ends that could be resolved to generate the genomic DNA by site-specific nicking, rearrangement, and ligation as has been proposed in thede novostart model of Baroudyet al.(B. M. Baroudy, S. Venkatesam, and B. Moss, 1982,Cold Spring Harbor Symp. Quant. Biol.47, 723–729) for the replication of vaccinia Virus DNA.
M Wienesen - One of the best experts on this subject based on the ideXlab platform.
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longitudinal analysis of varicella zoster Virus DNA on the ocular surface associated with herpes zoster ophthalmicus
American Journal of Ophthalmology, 2001Co-Authors: Michel J W Zaal, Hennie J Volkerdieben, M Wienesen, J Damaro, A KijlstraAbstract:Abstract PURPOSE: Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface of patients with herpes zoster ophthalmicus. METHODS: Clinical specimens were obtained from the bulbar conjunctival surface with a cotton-tipped swab at weekly intervals for 6 consecutive weeks from 21 patients with acute ophthalmic zoster with a skin rash duration of less than 7 days. All patients received oral valacyclovir 1000 mg three times daily for 10 days without additional corticosteroids. The swabs were analyzed by means of polymerase chain reaction for the presence of varicella-zoster Virus and herpes simplex Virus type 1 DNA. Conjunctival swabs were also obtained from a control group of 20 patients with cataract. RESULTS: On inclusion, varicella-zoster Virus DNA was present on the ocular surface of 19 of the 21 patients. Six varicella-zoster Virus DNA–positive patients had no signs of ocular inflammation. All control swabs were negative for both varicella-zoster Virus and herpes simplex Virus DNA. The duration of varicella-zoster Virus DNA detection from rash onset varied from 2 to 34 days. The number of days between the onset of herpes zoster skin rash and the latest positive varicella-zoster Virus DNA test was significantly longer in patients whose age was equal to or above the median age of 66 years than in the younger patients (Mann-Whitney test: P = .0004). At 6-week follow-up, all conjunctival swabs were negative for varicella-zoster Virus DNA. However, at that time, the eyes of seven patients were still inflamed. CONCLUSION: The duration of varicella-zoster Virus DNA shedding in herpes zoster ophthalmicus is highly variable and age dependent, and is probably related to the host immune response.
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Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface associated with herpes zoster ophthalmicus
American journal of ophthalmology, 2001Co-Authors: Michel J W Zaal, M Wienesen, Hennie J. Völker-dieben, J. D'amaro, A KijlstraAbstract:Abstract PURPOSE: Longitudinal analysis of varicella-zoster Virus DNA on the ocular surface of patients with herpes zoster ophthalmicus. METHODS: Clinical specimens were obtained from the bulbar conjunctival surface with a cotton-tipped swab at weekly intervals for 6 consecutive weeks from 21 patients with acute ophthalmic zoster with a skin rash duration of less than 7 days. All patients received oral valacyclovir 1000 mg three times daily for 10 days without additional corticosteroids. The swabs were analyzed by means of polymerase chain reaction for the presence of varicella-zoster Virus and herpes simplex Virus type 1 DNA. Conjunctival swabs were also obtained from a control group of 20 patients with cataract. RESULTS: On inclusion, varicella-zoster Virus DNA was present on the ocular surface of 19 of the 21 patients. Six varicella-zoster Virus DNA–positive patients had no signs of ocular inflammation. All control swabs were negative for both varicella-zoster Virus and herpes simplex Virus DNA. The duration of varicella-zoster Virus DNA detection from rash onset varied from 2 to 34 days. The number of days between the onset of herpes zoster skin rash and the latest positive varicella-zoster Virus DNA test was significantly longer in patients whose age was equal to or above the median age of 66 years than in the younger patients (Mann-Whitney test: P = .0004). At 6-week follow-up, all conjunctival swabs were negative for varicella-zoster Virus DNA. However, at that time, the eyes of seven patients were still inflamed. CONCLUSION: The duration of varicella-zoster Virus DNA shedding in herpes zoster ophthalmicus is highly variable and age dependent, and is probably related to the host immune response.
