The Experts below are selected from a list of 37488 Experts worldwide ranked by ideXlab platform
L. Grillner - One of the best experts on this subject based on the ideXlab platform.
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Detection of herpes simplex Virus type 1, herpes simplex Virus type 2 and varicella-zoster Virus in skin lesions. Comparison of real-time PCR, nested PCR and Virus Isolation
Journal of Clinical Virology, 2004Co-Authors: Julia Schmutzhard, Hilde Merete Riedel, Benita Zweygberg Wirgart, L. GrillnerAbstract:Background: Herpes simplex Virus type 1 (HSV-1), herpes simplex Virus type 2 (HSV-2) and varicella-zoster Virus (VZV) cause a wide range of signs and symptoms, varying from trivial mucocutaneous lesions to life-threatening infections, especially in immuno-suppressed patients. Since antiviral drugs are available, rapid and sensitive laboratory diagnosis of these Virus infections is important. Objective: To set up and evaluate HSV-1, HSV-2 and VZV qualitative real-time PCR on the Lightcycler system and to compare the results with those of the 'in-house' nested PCR and Virus Isolation. Study design: 110 consecutive samples from dermal or genital lesions from patients suspected of having HSV infections and another 110 samples from patients with suspected VZV infections were tested with real-time PCR, nested PCR and Virus Isolation. Results: 24 samples (22%) were positive for HSV-1 by Virus Isolation and nested PCR, whereas 26 (24%) were positive by real-time PCR. HSV-2 was detected in 28 samples (25%) by Virus Isolation, in 41 (37%) by nested PCR and in 40 (36%) by real-time PCR. VZV was isolated in 15 samples (14%) and VZV DNA was detected in 51 samples (46%) by nested PCR as well as by real-time PCR. Nucleic acid amplification increased the detection rate of HSV-2 and VZV DNA in particular compared to Virus Isolation. No significant difference in sensitivity was found between real-time PCR and nested PCR. Conclusion: Real-time PCR has the advantage of rapid amplification, a reduced risk for contamination and it is a suitable method for diagnosis of VZV and HSV in specimens from skin lesions. © 2003 Elsevier B.V. All rights reserved.
B Wirgart Zweygberg - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous detection and typing of influenza Viruses A and B by a nested reverse transcription-PCR: Comparison to Virus Isolation and antigen detection by immunofluorescence and optimal immunoassay
2001Co-Authors: Björn Herrmann, C Larsson, B Wirgart ZweygbergAbstract:Simultaneous detection and typing of influenza Viruses A and B by a nested reverse transcription-PCR: Comparison to Virus Isolation and antigen detection by immunofluorescence and optimal immunoassay
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simultaneous detection and typing of influenza Viruses a and b by a nested reverse transcription pcr comparison to Virus Isolation and antigen detection by immunofluorescence and optical immunoassay flu oia
Journal of Clinical Microbiology, 2001Co-Authors: Björn Herrmann, C Larsson, B Wirgart ZweygbergAbstract:A nested reverse transcription (RT)-PCR was developed for simultaneous detection and typing of influenza Viruses A and B. The detection limit for influenza Virus A subtypes H1 and H3 and that for influenza Virus B were between 1 and 4 target gene copies per reaction for each type. The clinical benefit of the RT-PCR method was evaluated by comparing the results with Virus Isolation and direct immunofluorescence (IF) assays on 215 nasopharyngeal aspirates from patients with suspected influenza Virus infection. The RT-PCR detected 83 cases of influenza A, compared to 66 cases detected by Virus Isolation and 68 cases detected by IF assay. The corresponding figures for the detection of influenza B were 15, 12, and 11 cases, respectively. In total, 16 out of 98 RT-PCR-positive specimens were negative by Virus Isolation and IF. An optical immunoassay for rapid detection of influenza A and B (FLU OIA; Bio Star Inc., Boulder, Colo.) was compared to RT-PCR and IF on 105 nasopharyngeal aspirates and 79 swabs. The sensitivity for the OIA was 40.4% compared to PCR and 48.8% compared to IF assay, when nasopharyngeal aspirates were examined. The specificities were 94.3 and 93.9%, respectively. The sensitivity was higher for OIA on nasopharyngeal swabs, 77.5% and 86.6% compared to PCR and IF, respectively, while the specificity was lower, 82.0% and 75.5%, respectively. The RT-PCR provides a sensitive and specific method for detecting and typing influenza Viruses A and B. The rapid OIA is useful as a complementary test, but it cannot replace established methods without further evaluation.
Gordon Allan - One of the best experts on this subject based on the ideXlab platform.
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The reverse transcription polymerase chain reaction for the diagnosis of porcine reproductive and respiratory syndrome: Comparison with Virus Isolation and serology
Veterinary microbiology, 1998Co-Authors: M Spagnuolo-weaver, I. Walker, Francis Mcneilly, V. Calvert, D Graham, K Burns, B. M. Adair, Gordon AllanAbstract:A single-tube reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of porcine reproductive and respiratory syndrome (PRRS) Virus in blood samples from infected pigs was developed. This test was assessed for sensitivity and application as a rapid diagnostic tool by comparison with Virus Isolation and detection of PRRS Virus antibody in blood. The RT-PCR test was slightly more sensitive than Virus Isolation for detection of Virus in serum and markedly more sensitive than Virus Isolation from plasma from experimentally infected pigs. The RT-PCR test was also applicable when using whole blood-impregnated filter paper discs, with 94% of the specimens taken by this procedure being positive when compared to RT-PCR performed on serum. PRRS viral nucleic acid was detected in blood samples as early as 24 h after infection and persisted for some time, whereas circulating antibody to PRRS Virus was not detected in the same animals until 9 days after infection. These results indicate that the RT-PCR may be an useful technique for the early identification of PRRS viral nucleic acid in blood samples of infected pigs.
Julia Schmutzhard - One of the best experts on this subject based on the ideXlab platform.
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Detection of herpes simplex Virus type 1, herpes simplex Virus type 2 and varicella-zoster Virus in skin lesions. Comparison of real-time PCR, nested PCR and Virus Isolation
Journal of Clinical Virology, 2004Co-Authors: Julia Schmutzhard, Hilde Merete Riedel, Benita Zweygberg Wirgart, L. GrillnerAbstract:Background: Herpes simplex Virus type 1 (HSV-1), herpes simplex Virus type 2 (HSV-2) and varicella-zoster Virus (VZV) cause a wide range of signs and symptoms, varying from trivial mucocutaneous lesions to life-threatening infections, especially in immuno-suppressed patients. Since antiviral drugs are available, rapid and sensitive laboratory diagnosis of these Virus infections is important. Objective: To set up and evaluate HSV-1, HSV-2 and VZV qualitative real-time PCR on the Lightcycler system and to compare the results with those of the 'in-house' nested PCR and Virus Isolation. Study design: 110 consecutive samples from dermal or genital lesions from patients suspected of having HSV infections and another 110 samples from patients with suspected VZV infections were tested with real-time PCR, nested PCR and Virus Isolation. Results: 24 samples (22%) were positive for HSV-1 by Virus Isolation and nested PCR, whereas 26 (24%) were positive by real-time PCR. HSV-2 was detected in 28 samples (25%) by Virus Isolation, in 41 (37%) by nested PCR and in 40 (36%) by real-time PCR. VZV was isolated in 15 samples (14%) and VZV DNA was detected in 51 samples (46%) by nested PCR as well as by real-time PCR. Nucleic acid amplification increased the detection rate of HSV-2 and VZV DNA in particular compared to Virus Isolation. No significant difference in sensitivity was found between real-time PCR and nested PCR. Conclusion: Real-time PCR has the advantage of rapid amplification, a reduced risk for contamination and it is a suitable method for diagnosis of VZV and HSV in specimens from skin lesions. © 2003 Elsevier B.V. All rights reserved.
Björn Herrmann - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous detection and typing of influenza Viruses A and B by a nested reverse transcription-PCR: Comparison to Virus Isolation and antigen detection by immunofluorescence and optimal immunoassay
2001Co-Authors: Björn Herrmann, C Larsson, B Wirgart ZweygbergAbstract:Simultaneous detection and typing of influenza Viruses A and B by a nested reverse transcription-PCR: Comparison to Virus Isolation and antigen detection by immunofluorescence and optimal immunoassay
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simultaneous detection and typing of influenza Viruses a and b by a nested reverse transcription pcr comparison to Virus Isolation and antigen detection by immunofluorescence and optical immunoassay flu oia
Journal of Clinical Microbiology, 2001Co-Authors: Björn Herrmann, C Larsson, B Wirgart ZweygbergAbstract:A nested reverse transcription (RT)-PCR was developed for simultaneous detection and typing of influenza Viruses A and B. The detection limit for influenza Virus A subtypes H1 and H3 and that for influenza Virus B were between 1 and 4 target gene copies per reaction for each type. The clinical benefit of the RT-PCR method was evaluated by comparing the results with Virus Isolation and direct immunofluorescence (IF) assays on 215 nasopharyngeal aspirates from patients with suspected influenza Virus infection. The RT-PCR detected 83 cases of influenza A, compared to 66 cases detected by Virus Isolation and 68 cases detected by IF assay. The corresponding figures for the detection of influenza B were 15, 12, and 11 cases, respectively. In total, 16 out of 98 RT-PCR-positive specimens were negative by Virus Isolation and IF. An optical immunoassay for rapid detection of influenza A and B (FLU OIA; Bio Star Inc., Boulder, Colo.) was compared to RT-PCR and IF on 105 nasopharyngeal aspirates and 79 swabs. The sensitivity for the OIA was 40.4% compared to PCR and 48.8% compared to IF assay, when nasopharyngeal aspirates were examined. The specificities were 94.3 and 93.9%, respectively. The sensitivity was higher for OIA on nasopharyngeal swabs, 77.5% and 86.6% compared to PCR and IF, respectively, while the specificity was lower, 82.0% and 75.5%, respectively. The RT-PCR provides a sensitive and specific method for detecting and typing influenza Viruses A and B. The rapid OIA is useful as a complementary test, but it cannot replace established methods without further evaluation.
