The Experts below are selected from a list of 87 Experts worldwide ranked by ideXlab platform
Satoru Suzuki - One of the best experts on this subject based on the ideXlab platform.
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Virus-Like Particles Suppress Growth of the Red-Tide-Forming Marine Dinoflagellate Gymnodinium mikimotoi
Marine Biotechnology, 2003Co-Authors: Masashi Onji, Shin-ichi Nakano, Satoru SuzukiAbstract:We isolated 2 Virus-Like Agents that suppressed growth of Gymnodinium mikimotoi from coastal waters of the Uwa Sea, Japan. The Agents found in the flagellate cells, named GM6 and GM7, were filterable in a 0.22-lm-pore filter with approximately 100-nm shapes. Electron microscopic observation showed the presence of Virus-Like particles in severely damaged G. mikimotoi cells infected by GM6. The growth-suppression activity of the Agents (GM6 or GM7) was lost by heating at 50°C, with treatments of DNase and protease, and filtration through a 0.05-lm filter. Our results suggest that the Agents are DNA Viruses infectious to and virulent for G. mikimotoi. This is the first report of a Virus-Like Agent specific to G. mikimotoi .
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Virus-Like particles suppress growth of the red-tide-forming marine dinoflagellate Gymnodinium mikimotoi.
Marine Biotechnology, 2003Co-Authors: Masashi Onji, Shin-ichi Nakano, Satoru SuzukiAbstract:We isolated 2 Virus-Like Agents that suppressed growth of Gymnodinium mikimotoi from coastal waters of the Uwa Sea, Japan. The Agents found in the flagellate cells, named GM6 and GM7, were filterable in a 0.22-lm-pore filter with approximately 100-nm shapes. Electron microscopic observation showed the presence of Virus-Like particles in severely damaged G. mikimotoi cells infected by GM6. The growth-suppression activity of the Agents (GM6 or GM7) was lost by heating at 50°C, with treatments of DNase and protease, and filtration through a 0.05-lm filter. Our results suggest that the Agents are DNA Viruses infectious to and virulent for G. mikimotoi. This is the first report of a Virus-Like Agent specific to G. mikimotoi.
Masashi Onji - One of the best experts on this subject based on the ideXlab platform.
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Virus-Like Particles Suppress Growth of the Red-Tide-Forming Marine Dinoflagellate Gymnodinium mikimotoi
Marine Biotechnology, 2003Co-Authors: Masashi Onji, Shin-ichi Nakano, Satoru SuzukiAbstract:We isolated 2 Virus-Like Agents that suppressed growth of Gymnodinium mikimotoi from coastal waters of the Uwa Sea, Japan. The Agents found in the flagellate cells, named GM6 and GM7, were filterable in a 0.22-lm-pore filter with approximately 100-nm shapes. Electron microscopic observation showed the presence of Virus-Like particles in severely damaged G. mikimotoi cells infected by GM6. The growth-suppression activity of the Agents (GM6 or GM7) was lost by heating at 50°C, with treatments of DNase and protease, and filtration through a 0.05-lm filter. Our results suggest that the Agents are DNA Viruses infectious to and virulent for G. mikimotoi. This is the first report of a Virus-Like Agent specific to G. mikimotoi .
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Virus-Like particles suppress growth of the red-tide-forming marine dinoflagellate Gymnodinium mikimotoi.
Marine Biotechnology, 2003Co-Authors: Masashi Onji, Shin-ichi Nakano, Satoru SuzukiAbstract:We isolated 2 Virus-Like Agents that suppressed growth of Gymnodinium mikimotoi from coastal waters of the Uwa Sea, Japan. The Agents found in the flagellate cells, named GM6 and GM7, were filterable in a 0.22-lm-pore filter with approximately 100-nm shapes. Electron microscopic observation showed the presence of Virus-Like particles in severely damaged G. mikimotoi cells infected by GM6. The growth-suppression activity of the Agents (GM6 or GM7) was lost by heating at 50°C, with treatments of DNase and protease, and filtration through a 0.05-lm filter. Our results suggest that the Agents are DNA Viruses infectious to and virulent for G. mikimotoi. This is the first report of a Virus-Like Agent specific to G. mikimotoi.
John Cason - One of the best experts on this subject based on the ideXlab platform.
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a human murine mammary tumour Virus Like Agent is an unconvincing aetiological Agent for human breast cancer
Reviews in Medical Virology, 2004Co-Authors: Christine Mant, John CasonAbstract:There has recently been renewed interest in both the scientific literature, and in the media, that a human relative (HMLV) of murine mammary tumour Virus (MMTV) may be implicated in the aetiology of up to 42% of sporadic cases of human breast cancer. Such reports are potentially of considerable clinical significance, as aside from the small percentage of genetically acquired breast cancers, the cause of sporadic cases of breast cancer is completely unknown. Indeed, convincing proof of an infectious cause for human breast malignancies would permit the development of new preventative measures, treatment modalities and raise the possibility of prophylactic and therapeutic vaccines. Here we review the historical background for a retroviral cause of human breast cancers and give an up-to-date critique of the most recent research findings. We conclude that the available evidence for an infectious aetiology of human breast cancers is unconvincing. Amongst the many cognate arguments against an infective hypothesis for sporadic cases of human breast cancer are the facts that: (i) human tissues lack the appropriate cell-surface receptor for entry of MMTVs/HMLVs; (ii) unLike all other virally caused human malignancies, immunodeficiency does not predispose to an increased incidence, or prevalence, of human breast cancers; and, (iii) reports of PCR amplification of MMTV/HMLV sequences from breast cancers have been robustly disputed by four independent laboratories. Indeed positive PCR results may be readily explained by the mis-amplification of host genomic DNA. Hence, the burden of proof currently lies with those who champion a viral cause for sporadic cases of breast cancer. Copyright © 2004 John Wiley & Sons, Ltd.
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A human murine mammary tumour Virus‐Like Agent is an unconvincing aetiological Agent for human breast cancer
Reviews in Medical Virology, 2004Co-Authors: Christine Mant, John CasonAbstract:There has recently been renewed interest in both the scientific literature, and in the media, that a human relative (HMLV) of murine mammary tumour Virus (MMTV) may be implicated in the aetiology of up to 42% of sporadic cases of human breast cancer. Such reports are potentially of considerable clinical significance, as aside from the small percentage of genetically acquired breast cancers, the cause of sporadic cases of breast cancer is completely unknown. Indeed, convincing proof of an infectious cause for human breast malignancies would permit the development of new preventative measures, treatment modalities and raise the possibility of prophylactic and therapeutic vaccines. Here we review the historical background for a retroviral cause of human breast cancers and give an up-to-date critique of the most recent research findings. We conclude that the available evidence for an infectious aetiology of human breast cancers is unconvincing. Amongst the many cognate arguments against an infective hypothesis for sporadic cases of human breast cancer are the facts that: (i) human tissues lack the appropriate cell-surface receptor for entry of MMTVs/HMLVs; (ii) unLike all other virally caused human malignancies, immunodeficiency does not predispose to an increased incidence, or prevalence, of human breast cancers; and, (iii) reports of PCR amplification of MMTV/HMLV sequences from breast cancers have been robustly disputed by four independent laboratories. Indeed positive PCR results may be readily explained by the mis-amplification of host genomic DNA. Hence, the burden of proof currently lies with those who champion a viral cause for sporadic cases of breast cancer. Copyright © 2004 John Wiley & Sons, Ltd.
Shin-ichi Nakano - One of the best experts on this subject based on the ideXlab platform.
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Virus-Like Particles Suppress Growth of the Red-Tide-Forming Marine Dinoflagellate Gymnodinium mikimotoi
Marine Biotechnology, 2003Co-Authors: Masashi Onji, Shin-ichi Nakano, Satoru SuzukiAbstract:We isolated 2 Virus-Like Agents that suppressed growth of Gymnodinium mikimotoi from coastal waters of the Uwa Sea, Japan. The Agents found in the flagellate cells, named GM6 and GM7, were filterable in a 0.22-lm-pore filter with approximately 100-nm shapes. Electron microscopic observation showed the presence of Virus-Like particles in severely damaged G. mikimotoi cells infected by GM6. The growth-suppression activity of the Agents (GM6 or GM7) was lost by heating at 50°C, with treatments of DNase and protease, and filtration through a 0.05-lm filter. Our results suggest that the Agents are DNA Viruses infectious to and virulent for G. mikimotoi. This is the first report of a Virus-Like Agent specific to G. mikimotoi .
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Virus-Like particles suppress growth of the red-tide-forming marine dinoflagellate Gymnodinium mikimotoi.
Marine Biotechnology, 2003Co-Authors: Masashi Onji, Shin-ichi Nakano, Satoru SuzukiAbstract:We isolated 2 Virus-Like Agents that suppressed growth of Gymnodinium mikimotoi from coastal waters of the Uwa Sea, Japan. The Agents found in the flagellate cells, named GM6 and GM7, were filterable in a 0.22-lm-pore filter with approximately 100-nm shapes. Electron microscopic observation showed the presence of Virus-Like particles in severely damaged G. mikimotoi cells infected by GM6. The growth-suppression activity of the Agents (GM6 or GM7) was lost by heating at 50°C, with treatments of DNase and protease, and filtration through a 0.05-lm filter. Our results suggest that the Agents are DNA Viruses infectious to and virulent for G. mikimotoi. This is the first report of a Virus-Like Agent specific to G. mikimotoi.
Robert L. Gilbertson - One of the best experts on this subject based on the ideXlab platform.
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Tomato chocolate spot Virus, a member of a new torradoVirus species that causes a necrosis-associated disease of tomato in Guatemala
Archives of Virology, 2010Co-Authors: Ozgur Batuman, M. Palmieri, M. R. Rojas, Robert L. GilbertsonAbstract:Tomatoes in Guatemala have been affected by a new disease, locally known as “mancha de chocolate” (chocolate spot). The disease is characterized by distinct necrotic spots on leaves, stems and petioles that eventually expand and cause a dieback of apical tissues. Samples from symptomatic plants tested negative for infection by tomato spotted wilt Virus, tobacco streak Virus, tobacco etch Virus and other known tomato-infecting Viruses. A Virus-Like Agent was sap-transmitted from diseased tissue to Nicotiana benthamiana and, when graft-transmitted to tomato, this Agent induced chocolate spot symptoms. This Virus-Like Agent also was sap-transmitted to Datura stramonium and Nicotiana glutinosa, but not to a range of non-solanaceous indicator plants. Icosahedral virions ~28–30 nm in diameter were purified from symptomatic N. benthamiana plants. When rub-inoculated onto leaves of N. benthamiana plants, these virions induced symptoms indistinguishable from those in N. benthamiana plants infected with the sap-transmissible Virus associated with chocolate spot disease. Tomatoes inoculated with sap or grafted with shoots from N. benthamiana plants infected with purified virions developed typical chocolate spot symptoms, consistent with this Virus being the causal Agent of the disease. Analysis of nucleic acids associated with purified virions of the chocolate-spot-associated Virus, revealed a genome composed of two single-stranded RNAs of ~7.5 and ~5.1 kb. Sequence analysis of these RNAs revealed a genome organization similar to recently described torradoViruses, a new group of picorna-Like Viruses causing necrosis-associated diseases of tomatoes in Europe [tomato torrado Virus (ToTV)] and Mexico [tomato apex necrosis Virus (ToANV) and tomato marchitez Virus (ToMarV)]. Thus, the ~7.5 kb and ~5.1 kb RNAs of the chocolate-spot-associated Virus corresponded to the torradoVirus RNA1 and RNA2, respectively; however, sequence comparisons revealed 64–83% identities with RNA1 and RNA2 sequences of ToTV, ToANV and ToMarV. Together, these results indicate that the chocolate-spot-associated Virus is a member of a distinct torradoVirus species and, thus, another member of the recently established genus TorradoVirus in the family Secoviridae . The name tomato chocolate spot Virus is proposed.
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an outbreak of a necrosis disease of tomato in california in 2008 was caused by a new ilarVirus species related to parietaria mottle Virus
Plant Disease, 2009Co-Authors: Ozgur Batuman, G Miyao, L F Chen, R M Davis, Robert L. GilbertsonAbstract:During the 2008 early-summer growing season, Virus-Like necrosis symptoms, most similar to those induced by Tobacco streak Virus (TSV), were observed in leaves, stems, and petioles of processing tomato plants in the Central Valley of California. Symptoms were observed in numerous fields in Merced, San Joaquin, and Yolo counties, though the incidence of the disease in most fields was not high (not more than 5% but over 20% in some areas). Antibody-based tests of representative samples of the disease for infection with Tomato spotted wilt Virus, TSV, and Tomato apex necrosis Virus, which cause similar symptoms, were negative. A putative Virus-Like Agent was sap- and graft-transmitted to tomato plants and induced necrotic spots in leaves and stem and petiole necrosis symptoms that were similar to those observed in the field. Eventually, these plants recovered from these symptoms. In sap-transmission experiments, the Virus-Like Agent induced systemic symptoms in Chenopodium quinoa and C. amaranticolor (stunte...
