The Experts below are selected from a list of 141 Experts worldwide ranked by ideXlab platform
Masami Mochizuki - One of the best experts on this subject based on the ideXlab platform.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis . Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus. Methods Longicin P1 and P4 peptides were chemically synthesized. Antiviral activity of the longicin peptides against LGTV was evaluated through in vitro virucidal assays, wherein the antiviral efficacy was determined by reduction in number of viral foci and Virus yield. Additionally, longicin P4 was also tested for its activity against human adenoVirus, a non-enveloped Virus. Lastly, to assess the importance of longicin on the innate antiviral immunity of H. longicornis ticks, gene silencing through RNAi was performed. Results Longicin P4 produced significant viral foci reduction and lower Virus yield against LGTV, while longicin P1 failed to demonstrate the same results. Conversely, both longicin partial analogs (P1 and P4) did not show significant antiviral activity when tested on adenoVirus. In addition, longicin -silenced ticks showed significantly higher Virus titer after 7 days post-infection but a significantly lower titer was detected after an additional 14 days of observation as compared to the Luc dsRNA-injected ticks. Mortality in both groups did not show any significant difference. Conclusion Our results suggest that longicin P4 has in vitro antiviral activity against LGTV but not against a non-enveloped Virus such as adenoVirus. Likewise, though most cationic antimicrobial peptides like longicin act directly on target membranes, the exact mechanism of membrane targeting of longicin P4 in enveloped Viruses, such as LGTV, requires further investigation. Lastly, while the in vitro virucidal capacity of longicin P4 was confirmed in this study, the role of the endogenous tick longicin in the antiviral defense of H. longicornis against LGTV still remains to be demonstrated.
Melbourne Rio Talactac - One of the best experts on this subject based on the ideXlab platform.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis . Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus. Methods Longicin P1 and P4 peptides were chemically synthesized. Antiviral activity of the longicin peptides against LGTV was evaluated through in vitro virucidal assays, wherein the antiviral efficacy was determined by reduction in number of viral foci and Virus yield. Additionally, longicin P4 was also tested for its activity against human adenoVirus, a non-enveloped Virus. Lastly, to assess the importance of longicin on the innate antiviral immunity of H. longicornis ticks, gene silencing through RNAi was performed. Results Longicin P4 produced significant viral foci reduction and lower Virus yield against LGTV, while longicin P1 failed to demonstrate the same results. Conversely, both longicin partial analogs (P1 and P4) did not show significant antiviral activity when tested on adenoVirus. In addition, longicin -silenced ticks showed significantly higher Virus titer after 7 days post-infection but a significantly lower titer was detected after an additional 14 days of observation as compared to the Luc dsRNA-injected ticks. Mortality in both groups did not show any significant difference. Conclusion Our results suggest that longicin P4 has in vitro antiviral activity against LGTV but not against a non-enveloped Virus such as adenoVirus. Likewise, though most cationic antimicrobial peptides like longicin act directly on target membranes, the exact mechanism of membrane targeting of longicin P4 in enveloped Viruses, such as LGTV, requires further investigation. Lastly, while the in vitro virucidal capacity of longicin P4 was confirmed in this study, the role of the endogenous tick longicin in the antiviral defense of H. longicornis against LGTV still remains to be demonstrated.
Remil Linggatong Galay - One of the best experts on this subject based on the ideXlab platform.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis . Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus. Methods Longicin P1 and P4 peptides were chemically synthesized. Antiviral activity of the longicin peptides against LGTV was evaluated through in vitro virucidal assays, wherein the antiviral efficacy was determined by reduction in number of viral foci and Virus yield. Additionally, longicin P4 was also tested for its activity against human adenoVirus, a non-enveloped Virus. Lastly, to assess the importance of longicin on the innate antiviral immunity of H. longicornis ticks, gene silencing through RNAi was performed. Results Longicin P4 produced significant viral foci reduction and lower Virus yield against LGTV, while longicin P1 failed to demonstrate the same results. Conversely, both longicin partial analogs (P1 and P4) did not show significant antiviral activity when tested on adenoVirus. In addition, longicin -silenced ticks showed significantly higher Virus titer after 7 days post-infection but a significantly lower titer was detected after an additional 14 days of observation as compared to the Luc dsRNA-injected ticks. Mortality in both groups did not show any significant difference. Conclusion Our results suggest that longicin P4 has in vitro antiviral activity against LGTV but not against a non-enveloped Virus such as adenoVirus. Likewise, though most cationic antimicrobial peptides like longicin act directly on target membranes, the exact mechanism of membrane targeting of longicin P4 in enveloped Viruses, such as LGTV, requires further investigation. Lastly, while the in vitro virucidal capacity of longicin P4 was confirmed in this study, the role of the endogenous tick longicin in the antiviral defense of H. longicornis against LGTV still remains to be demonstrated.
Kozo Fujisaki - One of the best experts on this subject based on the ideXlab platform.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis . Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus. Methods Longicin P1 and P4 peptides were chemically synthesized. Antiviral activity of the longicin peptides against LGTV was evaluated through in vitro virucidal assays, wherein the antiviral efficacy was determined by reduction in number of viral foci and Virus yield. Additionally, longicin P4 was also tested for its activity against human adenoVirus, a non-enveloped Virus. Lastly, to assess the importance of longicin on the innate antiviral immunity of H. longicornis ticks, gene silencing through RNAi was performed. Results Longicin P4 produced significant viral foci reduction and lower Virus yield against LGTV, while longicin P1 failed to demonstrate the same results. Conversely, both longicin partial analogs (P1 and P4) did not show significant antiviral activity when tested on adenoVirus. In addition, longicin -silenced ticks showed significantly higher Virus titer after 7 days post-infection but a significantly lower titer was detected after an additional 14 days of observation as compared to the Luc dsRNA-injected ticks. Mortality in both groups did not show any significant difference. Conclusion Our results suggest that longicin P4 has in vitro antiviral activity against LGTV but not against a non-enveloped Virus such as adenoVirus. Likewise, though most cationic antimicrobial peptides like longicin act directly on target membranes, the exact mechanism of membrane targeting of longicin P4 in enveloped Viruses, such as LGTV, requires further investigation. Lastly, while the in vitro virucidal capacity of longicin P4 was confirmed in this study, the role of the endogenous tick longicin in the antiviral defense of H. longicornis against LGTV still remains to be demonstrated.
Naotoshi Tsuji - One of the best experts on this subject based on the ideXlab platform.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus.
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Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis Virus Surrogate Langat Virus
Parasites & Vectors, 2016Co-Authors: Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami MochizukiAbstract:Background Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis . Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat Virus (LGTV), a tick-borne flaviVirus. Methods Longicin P1 and P4 peptides were chemically synthesized. Antiviral activity of the longicin peptides against LGTV was evaluated through in vitro virucidal assays, wherein the antiviral efficacy was determined by reduction in number of viral foci and Virus yield. Additionally, longicin P4 was also tested for its activity against human adenoVirus, a non-enveloped Virus. Lastly, to assess the importance of longicin on the innate antiviral immunity of H. longicornis ticks, gene silencing through RNAi was performed. Results Longicin P4 produced significant viral foci reduction and lower Virus yield against LGTV, while longicin P1 failed to demonstrate the same results. Conversely, both longicin partial analogs (P1 and P4) did not show significant antiviral activity when tested on adenoVirus. In addition, longicin -silenced ticks showed significantly higher Virus titer after 7 days post-infection but a significantly lower titer was detected after an additional 14 days of observation as compared to the Luc dsRNA-injected ticks. Mortality in both groups did not show any significant difference. Conclusion Our results suggest that longicin P4 has in vitro antiviral activity against LGTV but not against a non-enveloped Virus such as adenoVirus. Likewise, though most cationic antimicrobial peptides like longicin act directly on target membranes, the exact mechanism of membrane targeting of longicin P4 in enveloped Viruses, such as LGTV, requires further investigation. Lastly, while the in vitro virucidal capacity of longicin P4 was confirmed in this study, the role of the endogenous tick longicin in the antiviral defense of H. longicornis against LGTV still remains to be demonstrated.
