The Experts below are selected from a list of 84 Experts worldwide ranked by ideXlab platform
Yvan Arsenijevic - One of the best experts on this subject based on the ideXlab platform.
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Remaining Rod Activity Mediates Visual Behavior in Adult Rpe65−/− mice.
Investigative ophthalmology & visual science, 2010Co-Authors: M. Cachafeiro, Alexis-pierre Bemelmans, Kriss Canola, V. Pignat, Sylvain V. Crippa, Corinne Kostic, Yvan ArsenijevicAbstract:PURPOSE C57/Bl6, Cpfl1(-/-) (cone photoreceptors function loss 1; pure rod function), Gnat1a(-/-) (rod α-transducin; pure cone function), and Rpe65(-/-);Rho(-/-) double-knockout mice were studied to distinguish the respective contributions of the different photoreceptor (PR) systems that enable light perception and mediate a Visual Reflex in adult Rpe65(-/-) mice, with a simple behavioral procedure. METHODS Visual function was estimated using a rotating automated optomotor drum covered with vertical black-and-white stripes at spatial frequencies of 0.025 to 0.5 cycles per degree (cyc/deg) in both photopic and scotopic conditions. Mouse strains with different luminances were tested to evaluate the contribution and the light-intensity threshold of each PR system. RESULTS Stripe rotation elicited head movements in the wild-type (WT) animals in photopic and scotopic conditions, depending on the spatial frequency, whereas the Cpfl1(-/-) mice show a reduced activity in the photopic condition and the Gnat1a(-/-) mice an almost absent response in the scotopic condition. A robust Visual response was obtained with Rpe65(-/-) knockout mice at 0.075 and 0.1 cyc/deg in the photopic condition. The residual rod function in the Rpe65(-/-) animals was demonstrated by testing Rpe65(-/-);Rho(-/-) mice that present no response in photopic conditions. CONCLUSIONS The optomotor test is a simple method of estimating the Visual function and evaluating the respective contributions of rod and cone systems. This test was used to demonstrate that in Rpe65(-/-) mice, devoid of functional cones and of detectable 11-cis-retinal protein, the rods mimic cone function in part, by mediating vision in photopic conditions.
Timothy Pheby - One of the best experts on this subject based on the ideXlab platform.
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schwann cell grafting into the retina of the dystrophic rcs rat limits functional deterioration
Investigative Ophthalmology & Visual Science, 2000Co-Authors: J. M. Lawrence, Yves Sauvé, David J Keegan, Peter J. Coffey, L Hetherington, S.j.o. Whiteley, Anthony S. L. Kwan, S V Girman, Timothy PhebyAbstract:PURPOSE: To examine whether congenic Schwann cells grafted into the subretinal space of dystrophic Royal College of Surgeons (RCS) rats can prevent photoreceptor loss and maintain Visual function. METHODS: Purified neonatal Schwann cells derived from congenic rats were grafted into the subretinal space of 3- to 4-week-old dystrophic RCS rats. Graft placement was confirmed using Schwann cells labeled in vitro with the fluorescent dye Hoechst 33342 or in grafted eyes processed for electron microscopy (48-hour to 1-month survival). At longer intervals, up to 9 months after surgery, animals were examined for photoreceptor survival; preservation of a Visual Reflex, head-tracking to moving stripes; and preservation of Visual receptive fields associated with the region of graft placement. RESULTS: One week after the graft was performed, Schwann cells had integrated into the subretinal space with little evidence of a reactive response. When screened for head-tracking to moving stripes, Schwann cell-grafted animals performed better than sham-treated or control dystrophic animals. Threshold sensitivity measurements and Visual field assessment made by recording from the superior colliculus also showed a significant level of preserved function compared with control animals. Functional rescue was correlated with photoreceptor survival and could be observed for at least 9 months after grafting. CONCLUSIONS: Schwann cells injected into the subretinal space limit functional deterioration and prolong photoreceptor survival. It is suggested that they act by local release of growth factors that either support photoreceptors directly and/or stimulate phagocytosis in RPE cells.
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Schwann cell grafting into the retina of the dystrophic RCS rat limits functional deterioration
Investigative ophthalmology & visual science, 2000Co-Authors: J. M. Lawrence, Yves Sauvé, David J Keegan, Peter J. Coffey, L Hetherington, S.j.o. Whiteley, Anthony S. L. Kwan, Timothy Pheby, Raymond D. LundAbstract:METHODS. Purified neonatal Schwann cells derived from congenic rats were grafted into the subretinal space of 3- to 4-week-old dystrophic RCS rats. Graft placement was confirmed using Schwann cells labeled in vitro with the fluorescent dye Hoechst 33342 or in grafted eyes processed for electron microscopy (48-hour to 1-month survival). At longer intervals, up to 9 months after surgery, animals were examined for photoreceptor survival; preservation of a Visual Reflex, headtracking to moving stripes; and preservation of Visual receptive fields associated with the region of graft placement. RESULTS. One week after the graft was performed, Schwann cells had integrated into the subretinal space with little evidence of a reactive response. When screened for head-tracking to moving stripes, Schwann cell‐ grafted animals performed better than sham-treated or control dystrophic animals. Threshold sensitivity measurements and Visual field assessment made by recording from the superior colliculus also showed a significant level of preserved function compared with control animals. Functional rescue was correlated with photoreceptor survival and could be observed for at least 9 months after grafting. CONCLUSIONS. Schwann cells injected into the subretinal space limit functional deterioration and prolong photoreceptor survival. It is suggested that they act by local release of growth factors that either support photoreceptors directly and/or stimulate phagocytosis in RPE cells. (Invest Ophthalmol Vis Sci. 2000;41:518 ‐528)
S.j.o. Whiteley - One of the best experts on this subject based on the ideXlab platform.
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schwann cell grafting into the retina of the dystrophic rcs rat limits functional deterioration
Investigative Ophthalmology & Visual Science, 2000Co-Authors: J. M. Lawrence, Yves Sauvé, David J Keegan, Peter J. Coffey, L Hetherington, S.j.o. Whiteley, Anthony S. L. Kwan, S V Girman, Timothy PhebyAbstract:PURPOSE: To examine whether congenic Schwann cells grafted into the subretinal space of dystrophic Royal College of Surgeons (RCS) rats can prevent photoreceptor loss and maintain Visual function. METHODS: Purified neonatal Schwann cells derived from congenic rats were grafted into the subretinal space of 3- to 4-week-old dystrophic RCS rats. Graft placement was confirmed using Schwann cells labeled in vitro with the fluorescent dye Hoechst 33342 or in grafted eyes processed for electron microscopy (48-hour to 1-month survival). At longer intervals, up to 9 months after surgery, animals were examined for photoreceptor survival; preservation of a Visual Reflex, head-tracking to moving stripes; and preservation of Visual receptive fields associated with the region of graft placement. RESULTS: One week after the graft was performed, Schwann cells had integrated into the subretinal space with little evidence of a reactive response. When screened for head-tracking to moving stripes, Schwann cell-grafted animals performed better than sham-treated or control dystrophic animals. Threshold sensitivity measurements and Visual field assessment made by recording from the superior colliculus also showed a significant level of preserved function compared with control animals. Functional rescue was correlated with photoreceptor survival and could be observed for at least 9 months after grafting. CONCLUSIONS: Schwann cells injected into the subretinal space limit functional deterioration and prolong photoreceptor survival. It is suggested that they act by local release of growth factors that either support photoreceptors directly and/or stimulate phagocytosis in RPE cells.
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Schwann cell grafting into the retina of the dystrophic RCS rat limits functional deterioration
Investigative ophthalmology & visual science, 2000Co-Authors: J. M. Lawrence, Yves Sauvé, David J Keegan, Peter J. Coffey, L Hetherington, S.j.o. Whiteley, Anthony S. L. Kwan, Timothy Pheby, Raymond D. LundAbstract:METHODS. Purified neonatal Schwann cells derived from congenic rats were grafted into the subretinal space of 3- to 4-week-old dystrophic RCS rats. Graft placement was confirmed using Schwann cells labeled in vitro with the fluorescent dye Hoechst 33342 or in grafted eyes processed for electron microscopy (48-hour to 1-month survival). At longer intervals, up to 9 months after surgery, animals were examined for photoreceptor survival; preservation of a Visual Reflex, headtracking to moving stripes; and preservation of Visual receptive fields associated with the region of graft placement. RESULTS. One week after the graft was performed, Schwann cells had integrated into the subretinal space with little evidence of a reactive response. When screened for head-tracking to moving stripes, Schwann cell‐ grafted animals performed better than sham-treated or control dystrophic animals. Threshold sensitivity measurements and Visual field assessment made by recording from the superior colliculus also showed a significant level of preserved function compared with control animals. Functional rescue was correlated with photoreceptor survival and could be observed for at least 9 months after grafting. CONCLUSIONS. Schwann cells injected into the subretinal space limit functional deterioration and prolong photoreceptor survival. It is suggested that they act by local release of growth factors that either support photoreceptors directly and/or stimulate phagocytosis in RPE cells. (Invest Ophthalmol Vis Sci. 2000;41:518 ‐528)
M. Cachafeiro - One of the best experts on this subject based on the ideXlab platform.
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Remaining Rod Activity Mediates Visual Behavior in Adult Rpe65−/− mice.
Investigative ophthalmology & visual science, 2010Co-Authors: M. Cachafeiro, Alexis-pierre Bemelmans, Kriss Canola, V. Pignat, Sylvain V. Crippa, Corinne Kostic, Yvan ArsenijevicAbstract:PURPOSE C57/Bl6, Cpfl1(-/-) (cone photoreceptors function loss 1; pure rod function), Gnat1a(-/-) (rod α-transducin; pure cone function), and Rpe65(-/-);Rho(-/-) double-knockout mice were studied to distinguish the respective contributions of the different photoreceptor (PR) systems that enable light perception and mediate a Visual Reflex in adult Rpe65(-/-) mice, with a simple behavioral procedure. METHODS Visual function was estimated using a rotating automated optomotor drum covered with vertical black-and-white stripes at spatial frequencies of 0.025 to 0.5 cycles per degree (cyc/deg) in both photopic and scotopic conditions. Mouse strains with different luminances were tested to evaluate the contribution and the light-intensity threshold of each PR system. RESULTS Stripe rotation elicited head movements in the wild-type (WT) animals in photopic and scotopic conditions, depending on the spatial frequency, whereas the Cpfl1(-/-) mice show a reduced activity in the photopic condition and the Gnat1a(-/-) mice an almost absent response in the scotopic condition. A robust Visual response was obtained with Rpe65(-/-) knockout mice at 0.075 and 0.1 cyc/deg in the photopic condition. The residual rod function in the Rpe65(-/-) animals was demonstrated by testing Rpe65(-/-);Rho(-/-) mice that present no response in photopic conditions. CONCLUSIONS The optomotor test is a simple method of estimating the Visual function and evaluating the respective contributions of rod and cone systems. This test was used to demonstrate that in Rpe65(-/-) mice, devoid of functional cones and of detectable 11-cis-retinal protein, the rods mimic cone function in part, by mediating vision in photopic conditions.
J. M. Lawrence - One of the best experts on this subject based on the ideXlab platform.
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schwann cell grafting into the retina of the dystrophic rcs rat limits functional deterioration
Investigative Ophthalmology & Visual Science, 2000Co-Authors: J. M. Lawrence, Yves Sauvé, David J Keegan, Peter J. Coffey, L Hetherington, S.j.o. Whiteley, Anthony S. L. Kwan, S V Girman, Timothy PhebyAbstract:PURPOSE: To examine whether congenic Schwann cells grafted into the subretinal space of dystrophic Royal College of Surgeons (RCS) rats can prevent photoreceptor loss and maintain Visual function. METHODS: Purified neonatal Schwann cells derived from congenic rats were grafted into the subretinal space of 3- to 4-week-old dystrophic RCS rats. Graft placement was confirmed using Schwann cells labeled in vitro with the fluorescent dye Hoechst 33342 or in grafted eyes processed for electron microscopy (48-hour to 1-month survival). At longer intervals, up to 9 months after surgery, animals were examined for photoreceptor survival; preservation of a Visual Reflex, head-tracking to moving stripes; and preservation of Visual receptive fields associated with the region of graft placement. RESULTS: One week after the graft was performed, Schwann cells had integrated into the subretinal space with little evidence of a reactive response. When screened for head-tracking to moving stripes, Schwann cell-grafted animals performed better than sham-treated or control dystrophic animals. Threshold sensitivity measurements and Visual field assessment made by recording from the superior colliculus also showed a significant level of preserved function compared with control animals. Functional rescue was correlated with photoreceptor survival and could be observed for at least 9 months after grafting. CONCLUSIONS: Schwann cells injected into the subretinal space limit functional deterioration and prolong photoreceptor survival. It is suggested that they act by local release of growth factors that either support photoreceptors directly and/or stimulate phagocytosis in RPE cells.
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Schwann cell grafting into the retina of the dystrophic RCS rat limits functional deterioration
Investigative ophthalmology & visual science, 2000Co-Authors: J. M. Lawrence, Yves Sauvé, David J Keegan, Peter J. Coffey, L Hetherington, S.j.o. Whiteley, Anthony S. L. Kwan, Timothy Pheby, Raymond D. LundAbstract:METHODS. Purified neonatal Schwann cells derived from congenic rats were grafted into the subretinal space of 3- to 4-week-old dystrophic RCS rats. Graft placement was confirmed using Schwann cells labeled in vitro with the fluorescent dye Hoechst 33342 or in grafted eyes processed for electron microscopy (48-hour to 1-month survival). At longer intervals, up to 9 months after surgery, animals were examined for photoreceptor survival; preservation of a Visual Reflex, headtracking to moving stripes; and preservation of Visual receptive fields associated with the region of graft placement. RESULTS. One week after the graft was performed, Schwann cells had integrated into the subretinal space with little evidence of a reactive response. When screened for head-tracking to moving stripes, Schwann cell‐ grafted animals performed better than sham-treated or control dystrophic animals. Threshold sensitivity measurements and Visual field assessment made by recording from the superior colliculus also showed a significant level of preserved function compared with control animals. Functional rescue was correlated with photoreceptor survival and could be observed for at least 9 months after grafting. CONCLUSIONS. Schwann cells injected into the subretinal space limit functional deterioration and prolong photoreceptor survival. It is suggested that they act by local release of growth factors that either support photoreceptors directly and/or stimulate phagocytosis in RPE cells. (Invest Ophthalmol Vis Sci. 2000;41:518 ‐528)
