The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform
José António Belo - One of the best experts on this subject based on the ideXlab platform.
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Matrix Gla Protein expression pattern in the early avian embryo
The International Journal of Developmental Biology, 2016Co-Authors: Elizabeth Correia, M. Leonor Cancela, Natércia Conceição, José António BeloAbstract:MGP (Matrix Gla Protein) is an extracellular matrix vitamin K dependent protein previously identified as a physiological inhibitor of calcification and shown to be well conserved among vertebrates during evolution. MGP is involved in other mechanisms such as TGF-beta and BMP activity, and a proposed modulator of cell-matrix interactions. MGP is expressed early in vertebrate development although its role has not been clarified. Previous work in the chicken embryo found MGP localization predominantly in the aorta and aortic valve base, but no data is available earlier in development. Here we examined MGP expression pattern using whole-mount in situ hybridization and histological sectioning during the initial stages of chick development. MGP was first detected at HH10 in the head and in the forming dorsal aorta. At the moment of the onset of blood circulation, MGP was expressed additionally in the venous plexus which will remodel into the Vitelline Arteries. By E2.25, it is clear that the Vitelline Arteries are MGP positive. MGP expression progresses centrifugally throughout the area vasculosa of the yolk sac. Between stages HH17 and HH19 MGP is seen in the dorsal aorta, heart, notochord, nephric duct, roof plate, Vitelline Arteries and in the yolk sac, beneath main arterial branches and in the vicinity of several vessels and venules. MGP expression persists in these areas at least until E4.5. These data suggest that MGP expression could be associated with cell migration and differentiation and to the onset of angiogenesis in the developing chick embryo. This data has biomedical relevance by pointing to the potential use of chick embryo explants to study molecules involved in artery calcification
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Matrix Gla Protein expression pattern in the early avian embryo.
The International Journal of Developmental Biology, 2015Co-Authors: Elizabeth Correia, M. Leonor Cancela, Natércia Conceição, José António BeloAbstract:MGP (Matrix Gla Protein) is an extracellular matrix vitamin K dependent protein previously identified as a physiological inhibitor of calcification and shown to be well conserved among vertebrates during evolution. MGP is involved in other mechanisms such as TGF-β and BMP activity, and a proposed modulator of cell–matrix interactions. MGP is expressed early in vertebrate development although its role has not been clarified. Previous work in the chicken embryo found MGP localization predominantly in the aorta and aortic valve base, but no data is available earlier in development. Here we examined MGP expression pattern using whole-mount in situ hybridization and histological sectioning during the initial stages of chick development. MGP was first detected at HH10 in the head and in the forming dorsal aorta. At the moment of the onset of blood circulation, MGP was expressed additionally in the venous plexus which will remodel into the Vitelline Arteries. By E2.25, it is clear that the Vitelline Arteries are MGP positive. MGP expression progresses centrifugally throughout the area vasculosa of the yolk sac. Between stages HH17 and HH19 MGP is seen in the dorsal aorta, heart, notochord, nephric duct, roof plate, Vitelline Arteries and in the yolk sac, beneath main arterial branches and in the vicinity of several vessels and venules. MGP expression persists in these areas at least until E4.5. These data suggest that MGP expression could be associated with cell migration and differentiation and to the onset of angiogenesis in the developing chick embryo. This data has biomedical relevance by pointing to the potential use of chick embryo explants to study molecules involved in artery calcification.
Mervin C. Yoder - One of the best experts on this subject based on the ideXlab platform.
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Biomechanical forces promote embryonic haematopoiesis
Nature, 2009Co-Authors: Luigi Adamo, Olaia Naveiras, Pamela L. Wenzel, Shannon Mckinney-freeman, Peter J. Mack, Jorge Gracia-sancho, Astrid Suchy-dicey, Momoko Yoshimoto, M. William Lensch, Mervin C. YoderAbstract:Biomechanical forces are emerging as critical regulators of embryogenesis, particularly in the developing cardiovascular system. After initiation of the heartbeat in vertebrates, cells lining the ventral aspect of the dorsal aorta, the placental vessels, and the umbilical and Vitelline Arteries initiate expression of the transcription factor Runx1 (refs 3-5), a master regulator of haematopoiesis, and give rise to haematopoietic cells. It remains unknown whether the biomechanical forces imposed on the vascular wall at this developmental stage act as a determinant of haematopoietic potential. Here, using mouse embryonic stem cells differentiated in vitro, we show that fluid shear stress increases the expression of Runx1 in CD41(+)c-Kit(+) haematopoietic progenitor cells, concomitantly augmenting their haematopoietic colony-forming potential. Moreover, we find that shear stress increases haematopoietic colony-forming potential and expression of haematopoietic markers in the para-aortic splanchnopleura/aorta-gonads-mesonephros of mouse embryos and that abrogation of nitric oxide, a mediator of shear-stress-induced signalling, compromises haematopoietic potential in vitro and in vivo. Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development.
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Biomechanical forces promote embryonic haematopoiesis. Nature 459, U1131-consis consisfluo-U1120
2009Co-Authors: Luigi Adamo, Olaia Naveiras, Pamela L. Wenzel, Shannon Mckinney-freeman, Jorge Gracia-sancho, Astrid Suchy-dicey, Momoko Yoshimoto, J. Mack, M. William, Mervin C. YoderAbstract:Biomechanical forces are emerging as critical regulators of embryogenesis, particularly in the developing cardiovascular system1,2. After initiation of the heartbeat in vertebrates, cells lining the ventral aspect of the dorsal aorta, the placental vessels, and the umbilical and Vitelline Arteries initiate expression of the transcription factor Runx1 (refs 3–5), a master regulator of haematopoiesis, and give rise to haematopoietic cells4. It remains unknown whether the biomechanical forces imposed on the vascular wall at this developmental stage act as a determinant of haematopoietic potential6. Here, using mouse embryonic stem cells differentiated in vitro, we show that fluid shear stress increases the expression of Runx1 in CD41+c-Kit+ haematopoietic progenitor cells7,concomitantly augmenting their haematopoietic colony-forming potential. Moreover, we find that shear stress increases haematopoietic colony-forming potential and expression of haematopoietic markers in the paraaortic splanchnopleura/aorta–gonads–mesonephros of mouse embryos and that abrogation of nitric oxide, a mediator of shear-stress-induced signalling8, compromises haematopoietic potential in vitro and in vivo. Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development
Elizabeth Correia - One of the best experts on this subject based on the ideXlab platform.
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Matrix Gla Protein expression pattern in the early avian embryo
The International Journal of Developmental Biology, 2016Co-Authors: Elizabeth Correia, M. Leonor Cancela, Natércia Conceição, José António BeloAbstract:MGP (Matrix Gla Protein) is an extracellular matrix vitamin K dependent protein previously identified as a physiological inhibitor of calcification and shown to be well conserved among vertebrates during evolution. MGP is involved in other mechanisms such as TGF-beta and BMP activity, and a proposed modulator of cell-matrix interactions. MGP is expressed early in vertebrate development although its role has not been clarified. Previous work in the chicken embryo found MGP localization predominantly in the aorta and aortic valve base, but no data is available earlier in development. Here we examined MGP expression pattern using whole-mount in situ hybridization and histological sectioning during the initial stages of chick development. MGP was first detected at HH10 in the head and in the forming dorsal aorta. At the moment of the onset of blood circulation, MGP was expressed additionally in the venous plexus which will remodel into the Vitelline Arteries. By E2.25, it is clear that the Vitelline Arteries are MGP positive. MGP expression progresses centrifugally throughout the area vasculosa of the yolk sac. Between stages HH17 and HH19 MGP is seen in the dorsal aorta, heart, notochord, nephric duct, roof plate, Vitelline Arteries and in the yolk sac, beneath main arterial branches and in the vicinity of several vessels and venules. MGP expression persists in these areas at least until E4.5. These data suggest that MGP expression could be associated with cell migration and differentiation and to the onset of angiogenesis in the developing chick embryo. This data has biomedical relevance by pointing to the potential use of chick embryo explants to study molecules involved in artery calcification
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Matrix Gla Protein expression pattern in the early avian embryo.
The International Journal of Developmental Biology, 2015Co-Authors: Elizabeth Correia, M. Leonor Cancela, Natércia Conceição, José António BeloAbstract:MGP (Matrix Gla Protein) is an extracellular matrix vitamin K dependent protein previously identified as a physiological inhibitor of calcification and shown to be well conserved among vertebrates during evolution. MGP is involved in other mechanisms such as TGF-β and BMP activity, and a proposed modulator of cell–matrix interactions. MGP is expressed early in vertebrate development although its role has not been clarified. Previous work in the chicken embryo found MGP localization predominantly in the aorta and aortic valve base, but no data is available earlier in development. Here we examined MGP expression pattern using whole-mount in situ hybridization and histological sectioning during the initial stages of chick development. MGP was first detected at HH10 in the head and in the forming dorsal aorta. At the moment of the onset of blood circulation, MGP was expressed additionally in the venous plexus which will remodel into the Vitelline Arteries. By E2.25, it is clear that the Vitelline Arteries are MGP positive. MGP expression progresses centrifugally throughout the area vasculosa of the yolk sac. Between stages HH17 and HH19 MGP is seen in the dorsal aorta, heart, notochord, nephric duct, roof plate, Vitelline Arteries and in the yolk sac, beneath main arterial branches and in the vicinity of several vessels and venules. MGP expression persists in these areas at least until E4.5. These data suggest that MGP expression could be associated with cell migration and differentiation and to the onset of angiogenesis in the developing chick embryo. This data has biomedical relevance by pointing to the potential use of chick embryo explants to study molecules involved in artery calcification.
Sang Joon Lee - One of the best experts on this subject based on the ideXlab platform.
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Thermal effect on heart rate and hemodynamics in Vitelline Arteries of stage 18 chicken embryos.
Journal of biomechanics, 2010Co-Authors: Jung Yeop Lee, Sang Joon LeeAbstract:Abstract We investigated the thermal effects on heart rate, hemodynamics, and response of Vitelline Arteries of stage-18 chicken embryos. Heart rate was monitored by a high-speed imaging method, while hemodynamic quantities were evaluated using a particle image velocimetry (PIV) technique. Experiments were carried out at seven different temperatures (36–42 °C with 1 °C interval) after 1 h of incubation to stabilize the heart rate. The heart rate increased in a linear manner (r=0.992). Due to the increased cardiac output (or heart rate), the hemodynamic quantities such as mean velocity (Umean), velocity fluctuation (Ufluc), and peak velocity (Upeak) also increased with respect to the Womersley number (Ω) in the manner r=0.599, 0.693, and 0.725, respectively. This indicates that the mechanical force exerting on the vessel walls increases. However, the active response (or regulation) of the Vitelline Arteries was not observed in this study.
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Hemodynamics of the omphalo-mesenteric Arteries in stage 18 chicken embryos and "flow-structure" relations for the microcirculation.
Microvascular research, 2010Co-Authors: Jung Yeop Lee, Sang Joon LeeAbstract:Abstract Objective The primary objective of this study is to evaluate the hemodynamic and structural characteristics of the omphalo-mesenteric (Vitelline) Arteries in stage 18 chicken embryos. The measured results were compared with Murray's law to validate the theoretical prediction on the vascular structure. Methods Variation of hemodynamic parameters such as mean velocity (Umean), peak velocity (Upeak) at the systolic phase, velocity fluctuations (Ufluc) at the pulsatile frequency, and the Womersley number (Ω) were measured with respect to the geometric parameters including the bifurcation cascade level (BCL), vessel diameter (D), and distance (L) from the first bifurcation. They were assessed by using the time-resolved in vivo micro-PIV (particle image velocimetry) technique and the geometric information was obtained from the microscopic vessel images. Results The effect of “branching of the vessel” on the variation of hemodynamic characteristics is similar to those of the “increase in distance” from the first bifurcation and the “decrease in vessel diameter”. The flow quantities (Umean, Upeak and Ufluc) decrease due to the increase in cross-sectional area ratio (γ = 1.209 = (∑ Ddaughter2) / Dmother2), and the Womersley number also decreases as the bifurcation cascades (Ω « 1). Conclusion The geometric parameters are closely related to the variation of hemodynamic characteristics. Murray's law with non-constant viscosity hypothesis would provide an insight on the two-phase nature of microvascular blood flows.
Luigi Adamo - One of the best experts on this subject based on the ideXlab platform.
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Biomechanical forces promote embryonic haematopoiesis
Nature, 2009Co-Authors: Luigi Adamo, Olaia Naveiras, Pamela L. Wenzel, Shannon Mckinney-freeman, Peter J. Mack, Jorge Gracia-sancho, Astrid Suchy-dicey, Momoko Yoshimoto, M. William Lensch, Mervin C. YoderAbstract:Biomechanical forces are emerging as critical regulators of embryogenesis, particularly in the developing cardiovascular system. After initiation of the heartbeat in vertebrates, cells lining the ventral aspect of the dorsal aorta, the placental vessels, and the umbilical and Vitelline Arteries initiate expression of the transcription factor Runx1 (refs 3-5), a master regulator of haematopoiesis, and give rise to haematopoietic cells. It remains unknown whether the biomechanical forces imposed on the vascular wall at this developmental stage act as a determinant of haematopoietic potential. Here, using mouse embryonic stem cells differentiated in vitro, we show that fluid shear stress increases the expression of Runx1 in CD41(+)c-Kit(+) haematopoietic progenitor cells, concomitantly augmenting their haematopoietic colony-forming potential. Moreover, we find that shear stress increases haematopoietic colony-forming potential and expression of haematopoietic markers in the para-aortic splanchnopleura/aorta-gonads-mesonephros of mouse embryos and that abrogation of nitric oxide, a mediator of shear-stress-induced signalling, compromises haematopoietic potential in vitro and in vivo. Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development.
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Biomechanical forces promote embryonic haematopoiesis. Nature 459, U1131-consis consisfluo-U1120
2009Co-Authors: Luigi Adamo, Olaia Naveiras, Pamela L. Wenzel, Shannon Mckinney-freeman, Jorge Gracia-sancho, Astrid Suchy-dicey, Momoko Yoshimoto, J. Mack, M. William, Mervin C. YoderAbstract:Biomechanical forces are emerging as critical regulators of embryogenesis, particularly in the developing cardiovascular system1,2. After initiation of the heartbeat in vertebrates, cells lining the ventral aspect of the dorsal aorta, the placental vessels, and the umbilical and Vitelline Arteries initiate expression of the transcription factor Runx1 (refs 3–5), a master regulator of haematopoiesis, and give rise to haematopoietic cells4. It remains unknown whether the biomechanical forces imposed on the vascular wall at this developmental stage act as a determinant of haematopoietic potential6. Here, using mouse embryonic stem cells differentiated in vitro, we show that fluid shear stress increases the expression of Runx1 in CD41+c-Kit+ haematopoietic progenitor cells7,concomitantly augmenting their haematopoietic colony-forming potential. Moreover, we find that shear stress increases haematopoietic colony-forming potential and expression of haematopoietic markers in the paraaortic splanchnopleura/aorta–gonads–mesonephros of mouse embryos and that abrogation of nitric oxide, a mediator of shear-stress-induced signalling8, compromises haematopoietic potential in vitro and in vivo. Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development
