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Shigehiko Kitano - One of the best experts on this subject based on the ideXlab platform.
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Elevation of the Vitreous Body concentrations of oxidative stress-responsive apoptosis-inducing protein (ORAIP) in proliferative diabetic retinopathy
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Yuta Suzuki, Takako Yao, Ko Okumura, Yoshinori Seko, Shigehiko KitanoAbstract:Purpose Oxidative stress has been implicated in the pathogenesis of various disorders, including diabetic retinopathy (DR). Oxidative stress-responsive apoptosis-inducing protein (ORAIP; a tyrosine-sulfated secreted form of eukaryotic translation initiation factor 5A [eIF5A]) is a recently discovered pro-apoptotic ligand that is secreted from cells in response to oxidative stress and induces apoptosis in an autocrine fashion. This study aimed to determine if ORAIP plays a role in DR. Methods To investigate the role of ORAIP in DR, we analyzed the levels of ORAIP in the Vitreous Body and their relationship with the extent of proliferative diabetic retinopathy (PDR). Enzyme-linked immunosorbent assay was used to quantify the levels of ORAIP, vascular endothelial growth factor (VEGF), C–C motif chemokine ligand 2 (CCL2), interleukin-6 (IL-6), and IL-8 in the Vitreous Body of 40 eyes from 28 patients with PDR and 11 patients with non-PDR (NPDR). We also analyzed the expression of ORAIP in insoluble proliferative tissues from Vitreous Body samples by immunofluorescent staining. Results The Vitreous Body concentration of ORAIP was significantly ( P = 0.0433) higher in the PDR group (52.26 ± 8.68 [mean ± SE] ng/mL, n = 29) than in the NPDR group (28.21 ± 7.30 ng/mL, n = 11). However, there were no significant correlations between the concentration of ORAIP and those of VEGF, IL-6, CCL2, or IL-8. ORAIP expression was observed in the insoluble proliferative tissues in Vitreous Body samples of most patients in the PDR group, whereas almost no expression of ORAIP was observed in patients in the NPDR group. Conclusions Our findings strongly suggest that ORAIP plays a role in oxidative stress-induced retinal injury and may be a sensitive diagnostic marker and a promising therapeutic target for oxidative stress-induced cytotoxicity.
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elevation of the Vitreous Body concentrations of oxidative stress responsive apoptosis inducing protein oraip in proliferative diabetic retinopathy
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Yuta Suzuki, Ko Okumura, Yoshinori Seko, Shigehiko KitanoAbstract:Oxidative stress has been implicated in the pathogenesis of various disorders, including diabetic retinopathy (DR). Oxidative stress-responsive apoptosis-inducing protein (ORAIP; a tyrosine-sulfated secreted form of eukaryotic translation initiation factor 5A [eIF5A]) is a recently discovered pro-apoptotic ligand that is secreted from cells in response to oxidative stress and induces apoptosis in an autocrine fashion. This study aimed to determine if ORAIP plays a role in DR. To investigate the role of ORAIP in DR, we analyzed the levels of ORAIP in the Vitreous Body and their relationship with the extent of proliferative diabetic retinopathy (PDR). Enzyme-linked immunosorbent assay was used to quantify the levels of ORAIP, vascular endothelial growth factor (VEGF), C–C motif chemokine ligand 2 (CCL2), interleukin-6 (IL-6), and IL-8 in the Vitreous Body of 40 eyes from 28 patients with PDR and 11 patients with non-PDR (NPDR). We also analyzed the expression of ORAIP in insoluble proliferative tissues from Vitreous Body samples by immunofluorescent staining. The Vitreous Body concentration of ORAIP was significantly (P = 0.0433) higher in the PDR group (52.26 ± 8.68 [mean ± SE] ng/mL, n = 29) than in the NPDR group (28.21 ± 7.30 ng/mL, n = 11). However, there were no significant correlations between the concentration of ORAIP and those of VEGF, IL-6, CCL2, or IL-8. ORAIP expression was observed in the insoluble proliferative tissues in Vitreous Body samples of most patients in the PDR group, whereas almost no expression of ORAIP was observed in patients in the NPDR group. Our findings strongly suggest that ORAIP plays a role in oxidative stress-induced retinal injury and may be a sensitive diagnostic marker and a promising therapeutic target for oxidative stress-induced cytotoxicity.
Tetsuro Oshika - One of the best experts on this subject based on the ideXlab platform.
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experimental use of estriol for visualizing the Vitreous Body in the anterior chamber after posterior capsule rupture in animal models
Journal of Cataract and Refractive Surgery, 2009Co-Authors: Rong Huang, Yuichi Kaji, Shinichi Fukuda, Tetsuro OshikaAbstract:Purpose To compare the efficacy and safety of estriol and triamcinolone acetonide suspensions in visualizing the prolapsed Vitreous Body in the anterior chamber after posterior capsule rupture in animal models. Setting Tsukuba University Institute of Clinical Medicine, Ibaraki, Japan. Methods To evaluate efficacy, triamcinolone acetonide or estriol suspension was injected into the anterior chambers of porcine eyes after intentional posterior capsule rupture. To evaluate safety, triamcinolone acetonide 5.0 mg or estriol in 0.1 mL suspension was injected into the anterior chamber of New Zealand white rabbits. Slitlamp examinations, intraocular pressure (IOP), corneal endothelial cell density (ECD) measurements, and histologic examinations were performed up to 28 days after the injection. Results Triamcinolone acetonide and estriol were equally effective in allowing visualization of the prolapsed Vitreous Body in the anterior chamber. The granules of triamcinolone acetonide or estriol disappeared 1 day after the injection and did not affect the IOP or corneal ECD. No statistically significant histological changes were observed in the eyes 28 days after the injection of triamcinolone acetonide or estriol. Conclusions Estriol was effective for the visualization of the prolapsed Vitreous Body in the anterior chamber after posterior capsule rupture. In experimental models, no significant side effects were observed after the injection of estriol in the anterior chamber. Results suggest that estriol is an alternative reagent for visualizing the Vitreous Body, especially in steroid responders, because it has no glucocorticoid or mineralocorticoid activity.
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visualizing the Vitreous Body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Ophthalmology, 2004Co-Authors: Yuichi Kaji, Fumiki Okamoto, Takahiro Hiraoka, M Sato, Beihua Hu, N Yamane, Tetsuro OshikaAbstract:Abstract Objective To develop a new technique to visualize Vitreous Body prolapsed in the anterior chamber using 11-deoxycortisol. Study design Experimental study. Methods An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the Vitreous Body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-deoxycortisol in the anterior chamber. Results In our posterior capsule rupture model, the Vitreous in the anterior chamber became clearly visible, with 11-deoxycortisol showing white particles entrapped on its surface. The injection of 11-deoxycortisol facilitated the complete removal of the Vitreous Body from the anterior chamber. In intact rabbit eyes, most of the injected 11-deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions The injection of 11-deoxycortisol in the anterior chamber is useful in visualizing the Vitreous Body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture.
Taiji Sakamoto - One of the best experts on this subject based on the ideXlab platform.
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polylactic acid for visualizing the Vitreous Body during vitrectomy
Investigative Ophthalmology & Visual Science, 2007Co-Authors: Toshifumi Yamashita, Taiji Sakamoto, Keita Yamakiri, Muneki Miura, Hiroshi Enaida, Akifumi Ueno, Ikuyo Atsumi, Keiichi Matsuhisa, Yuji Sakamoto, Tetsuo KidaAbstract:PURPOSE. To investigate the possibility of using polylactic acid (PLA) as a surgical adjuvant for visualizing the Vitreous Body during vitrectomy. METHODS. After a core vitrectomy, 1 mL of PLA suspension was injected into the rabbit Vitreous in two groups: group A, 2.5% PLA (n = 5), and group B, 1% PLA (n = 9). Vehicle injection instead of PLA was used as a control (group C, n = 5). The clinical signs and electroretinogram (ERG) were evaluated for 28 days, and histologic findings were evaluated on day 28. Next, intraocular pressure (IOP) after intracameral injection of a PLA suspension was evaluated in the rabbits (n = 6). Last, the visualization of the Vitreous Body by PLA suspension was evaluated during vitrectomy in monkey eyes (n = 4). RESULTS. The white granules of PLA disappeared from the Vitreous cavity in 10 eyes within 3 weeks; however, a small amount of PLA remained in four eyes for 4 weeks. Mild inflammation of the anterior chamber was observed in one eye in group B and 1 eye in group C. No cataract or retinal hemorrhage was found in any eyes. The amplitude of ERG on each time point did not differ between the groups. IOP remained within normal range except for the initial spike. Retinal structure was well preserved histologically. During vitrectomy in monkey eyes, the Vitreous Body was well visualized, and the posterior Vitreous separation was performed easily and safely. CONCLUSIONS. PLA can be a new surgical adjuvant to visualize the Vitreous Body during vitrectomy.
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intracameral triamcinolone helps to visualize and remove the Vitreous Body in anterior chamber in cataract surgery
American Journal of Ophthalmology, 2004Co-Authors: Keita Yamakiri, Eisuke Uchino, Katsuaki Kimura, Taiji SakamotoAbstract:Aim To study the effects of intracameral injection of triamcinolone acetonide on visualizing and removing the Vitreous Body from the anterior chamber in cataract surgery. Design Observational case series. Methods Six eyes of six patients had the posterior capsule ruptured and the Vitreous Body prolapsed or incarcerated into the anterior chamber during cataract surgery. To visualize Vitreous Body, triamcinolone acetonide solution was injected into the anterior chamber and the Vitreous Body was resected. The intraoperative findings, results, and complications were evaluated. Results Vitreous Body was well observed under surgical microscopy and was resected safely and completely. Minimum inflammation was observed postoperatively, and the patients obtained good visual acuity. No serious complications were found. One eye showed increased intraocular pressure (40 mm Hg), which was normalized by additional washing of the anterior chamber. Conclusions Appropriate use of intracameral triamcinolone acetonide is beneficial to visualize and remove the Vitreous Body from the anterior chamber during cataract surgery, and sufficient washing of the anterior chamber is necessary to avoid complications.
Yuta Suzuki - One of the best experts on this subject based on the ideXlab platform.
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Elevation of the Vitreous Body concentrations of oxidative stress-responsive apoptosis-inducing protein (ORAIP) in proliferative diabetic retinopathy
Graefe's Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Yuta Suzuki, Takako Yao, Ko Okumura, Yoshinori Seko, Shigehiko KitanoAbstract:Purpose Oxidative stress has been implicated in the pathogenesis of various disorders, including diabetic retinopathy (DR). Oxidative stress-responsive apoptosis-inducing protein (ORAIP; a tyrosine-sulfated secreted form of eukaryotic translation initiation factor 5A [eIF5A]) is a recently discovered pro-apoptotic ligand that is secreted from cells in response to oxidative stress and induces apoptosis in an autocrine fashion. This study aimed to determine if ORAIP plays a role in DR. Methods To investigate the role of ORAIP in DR, we analyzed the levels of ORAIP in the Vitreous Body and their relationship with the extent of proliferative diabetic retinopathy (PDR). Enzyme-linked immunosorbent assay was used to quantify the levels of ORAIP, vascular endothelial growth factor (VEGF), C–C motif chemokine ligand 2 (CCL2), interleukin-6 (IL-6), and IL-8 in the Vitreous Body of 40 eyes from 28 patients with PDR and 11 patients with non-PDR (NPDR). We also analyzed the expression of ORAIP in insoluble proliferative tissues from Vitreous Body samples by immunofluorescent staining. Results The Vitreous Body concentration of ORAIP was significantly ( P = 0.0433) higher in the PDR group (52.26 ± 8.68 [mean ± SE] ng/mL, n = 29) than in the NPDR group (28.21 ± 7.30 ng/mL, n = 11). However, there were no significant correlations between the concentration of ORAIP and those of VEGF, IL-6, CCL2, or IL-8. ORAIP expression was observed in the insoluble proliferative tissues in Vitreous Body samples of most patients in the PDR group, whereas almost no expression of ORAIP was observed in patients in the NPDR group. Conclusions Our findings strongly suggest that ORAIP plays a role in oxidative stress-induced retinal injury and may be a sensitive diagnostic marker and a promising therapeutic target for oxidative stress-induced cytotoxicity.
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elevation of the Vitreous Body concentrations of oxidative stress responsive apoptosis inducing protein oraip in proliferative diabetic retinopathy
Graefes Archive for Clinical and Experimental Ophthalmology, 2019Co-Authors: Yuta Suzuki, Ko Okumura, Yoshinori Seko, Shigehiko KitanoAbstract:Oxidative stress has been implicated in the pathogenesis of various disorders, including diabetic retinopathy (DR). Oxidative stress-responsive apoptosis-inducing protein (ORAIP; a tyrosine-sulfated secreted form of eukaryotic translation initiation factor 5A [eIF5A]) is a recently discovered pro-apoptotic ligand that is secreted from cells in response to oxidative stress and induces apoptosis in an autocrine fashion. This study aimed to determine if ORAIP plays a role in DR. To investigate the role of ORAIP in DR, we analyzed the levels of ORAIP in the Vitreous Body and their relationship with the extent of proliferative diabetic retinopathy (PDR). Enzyme-linked immunosorbent assay was used to quantify the levels of ORAIP, vascular endothelial growth factor (VEGF), C–C motif chemokine ligand 2 (CCL2), interleukin-6 (IL-6), and IL-8 in the Vitreous Body of 40 eyes from 28 patients with PDR and 11 patients with non-PDR (NPDR). We also analyzed the expression of ORAIP in insoluble proliferative tissues from Vitreous Body samples by immunofluorescent staining. The Vitreous Body concentration of ORAIP was significantly (P = 0.0433) higher in the PDR group (52.26 ± 8.68 [mean ± SE] ng/mL, n = 29) than in the NPDR group (28.21 ± 7.30 ng/mL, n = 11). However, there were no significant correlations between the concentration of ORAIP and those of VEGF, IL-6, CCL2, or IL-8. ORAIP expression was observed in the insoluble proliferative tissues in Vitreous Body samples of most patients in the PDR group, whereas almost no expression of ORAIP was observed in patients in the NPDR group. Our findings strongly suggest that ORAIP plays a role in oxidative stress-induced retinal injury and may be a sensitive diagnostic marker and a promising therapeutic target for oxidative stress-induced cytotoxicity.
Keita Yamakiri - One of the best experts on this subject based on the ideXlab platform.
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polylactic acid for visualizing the Vitreous Body during vitrectomy
Investigative Ophthalmology & Visual Science, 2007Co-Authors: Toshifumi Yamashita, Taiji Sakamoto, Keita Yamakiri, Muneki Miura, Hiroshi Enaida, Akifumi Ueno, Ikuyo Atsumi, Keiichi Matsuhisa, Yuji Sakamoto, Tetsuo KidaAbstract:PURPOSE. To investigate the possibility of using polylactic acid (PLA) as a surgical adjuvant for visualizing the Vitreous Body during vitrectomy. METHODS. After a core vitrectomy, 1 mL of PLA suspension was injected into the rabbit Vitreous in two groups: group A, 2.5% PLA (n = 5), and group B, 1% PLA (n = 9). Vehicle injection instead of PLA was used as a control (group C, n = 5). The clinical signs and electroretinogram (ERG) were evaluated for 28 days, and histologic findings were evaluated on day 28. Next, intraocular pressure (IOP) after intracameral injection of a PLA suspension was evaluated in the rabbits (n = 6). Last, the visualization of the Vitreous Body by PLA suspension was evaluated during vitrectomy in monkey eyes (n = 4). RESULTS. The white granules of PLA disappeared from the Vitreous cavity in 10 eyes within 3 weeks; however, a small amount of PLA remained in four eyes for 4 weeks. Mild inflammation of the anterior chamber was observed in one eye in group B and 1 eye in group C. No cataract or retinal hemorrhage was found in any eyes. The amplitude of ERG on each time point did not differ between the groups. IOP remained within normal range except for the initial spike. Retinal structure was well preserved histologically. During vitrectomy in monkey eyes, the Vitreous Body was well visualized, and the posterior Vitreous separation was performed easily and safely. CONCLUSIONS. PLA can be a new surgical adjuvant to visualize the Vitreous Body during vitrectomy.
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intracameral triamcinolone helps to visualize and remove the Vitreous Body in anterior chamber in cataract surgery
American Journal of Ophthalmology, 2004Co-Authors: Keita Yamakiri, Eisuke Uchino, Katsuaki Kimura, Taiji SakamotoAbstract:Aim To study the effects of intracameral injection of triamcinolone acetonide on visualizing and removing the Vitreous Body from the anterior chamber in cataract surgery. Design Observational case series. Methods Six eyes of six patients had the posterior capsule ruptured and the Vitreous Body prolapsed or incarcerated into the anterior chamber during cataract surgery. To visualize Vitreous Body, triamcinolone acetonide solution was injected into the anterior chamber and the Vitreous Body was resected. The intraoperative findings, results, and complications were evaluated. Results Vitreous Body was well observed under surgical microscopy and was resected safely and completely. Minimum inflammation was observed postoperatively, and the patients obtained good visual acuity. No serious complications were found. One eye showed increased intraocular pressure (40 mm Hg), which was normalized by additional washing of the anterior chamber. Conclusions Appropriate use of intracameral triamcinolone acetonide is beneficial to visualize and remove the Vitreous Body from the anterior chamber during cataract surgery, and sufficient washing of the anterior chamber is necessary to avoid complications.
