The Experts below are selected from a list of 4434 Experts worldwide ranked by ideXlab platform
Rita Van Ginderdeuren - One of the best experts on this subject based on the ideXlab platform.
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a new and standardized method to sample and analyse Vitreous biopsies in unsolved uveitis by the cellient automated cell block system
Acta Ophthalmologica, 2014Co-Authors: Joachim Van Calster, Rita Van GinderdeurenAbstract:Purpose In this prospective study a universal protocol for sampling and analyzing Vitreous Material was investigated. Vitreous biopsies are difficult to handle because of the paucity of cells and the gelatinous structure of the Vitreous. Histopathological analysis of the Vitreous is useful in difficult uveitis cases to differentiate uveitis from lymphoma or infection and to define the type of cellular reaction. Methods After isolation of Vitreous content by a single port biopsy or by a standardized 23G vitrectomy, 170 consecutive Vitreous samples were analyzed with the Cellient® tissue processor (Hologic). This machine is a fully automated processor starting from a specified container with PreservCyt® (fixative fluid) with cells to paraffin. Cytology was compared with fixatives Cytolyt® (contains a mucolytic agent) and Preservcyt®. Routine histochemical and immunostainings were evaluated. Results In 79 cases (46%) a single port biopsy and in 91 cases (54%) a tree port pars plana vitrectomy was performed. In 94% of the cases, sufficient Material was found for establishing the diagnosis. In 18%, a Cytolyt® wash was necessary to prevent clotting of the tubes in the Cellient® due to the viscosity of the sample. In 21% the diagnosis was an acute inflammation (presence of granulocytes), in 32% chronic active inflammation (presence of T-lymphocytes), in 35% low-grade inflammation (presence of CD68 cells, without T-lymphocytes); and in 5% a malignant process. In 9% a granulomatous inflammation was detected, in 5% glial tissue (floaters). In 6% no diagnosis was found. Conclusion A standardized protocol for sampling and handling Vitreous biopsies, fixing in PreservCyt® and processing by the Cellient® system gives a superior result in morphology, number of cells, and possibility of immuno-histochemical stainings. The diagnosis can be established or confirmed in more than 90% of cases.
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a new and standardized method to sample and analyse Vitreous samples by the cellient automated cell block system
Acta Ophthalmologica, 2013Co-Authors: Rita Van Ginderdeuren, Joachim Van Calster, Peter Stalmans, Joost Van Den OordAbstract:Purpose In this prospective study, a universal protocol for sampling and analysing Vitreous Material was investigated. Vitreous biopsies are difficult to handle because of the paucity of cells and the gelatinous structure of the Vitreous. Histopathological analysis of the Vitreous is useful in difficult uveitis cases to differentiate uveitis from lymphoma or infection and to define the type of cellular reaction. Methods Hundred consecutive Vitreous samples were analysed with the Cellient® tissue processor (Hologic). This machine is a fully automated processor starting from a specified container with PreservCyt® (fixative fluid) with cells to paraffin. Cytology was compared with fixatives Cytolyt® (contains a mucolyticum) and PreservCyt®. Routine histochemical and immunostainings were evaluated. Results In 92% of the cases, sufficient Material was found for diagnosis. In 14%, a Cytolyt® wash was necessary to prevent clotting of the tubes in the Cellient® due to the viscosity of the sample. In 23%, the diagnosis was an acute inflammation (presence of granulocytes); in 33%, chronic active inflammation (presence of T lymphocytes); in 33%, low-grade inflammation (presence of CD68 cells, without T lymphocytes); and in 3%, a malignant process. Conclusion A standardized protocol for sampling and handling Vitreous biopsies, fixing in PreservCyt® and processing by the Cellient® gives a satisfactory result in morphology, number of cells and possibility of immuno-histochemical stainings. The diagnosis can be established or confirmed in more than 90% of cases.
Joachim Van Calster - One of the best experts on this subject based on the ideXlab platform.
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a new and standardized method to sample and analyse Vitreous biopsies in unsolved uveitis by the cellient automated cell block system
Acta Ophthalmologica, 2014Co-Authors: Joachim Van Calster, Rita Van GinderdeurenAbstract:Purpose In this prospective study a universal protocol for sampling and analyzing Vitreous Material was investigated. Vitreous biopsies are difficult to handle because of the paucity of cells and the gelatinous structure of the Vitreous. Histopathological analysis of the Vitreous is useful in difficult uveitis cases to differentiate uveitis from lymphoma or infection and to define the type of cellular reaction. Methods After isolation of Vitreous content by a single port biopsy or by a standardized 23G vitrectomy, 170 consecutive Vitreous samples were analyzed with the Cellient® tissue processor (Hologic). This machine is a fully automated processor starting from a specified container with PreservCyt® (fixative fluid) with cells to paraffin. Cytology was compared with fixatives Cytolyt® (contains a mucolytic agent) and Preservcyt®. Routine histochemical and immunostainings were evaluated. Results In 79 cases (46%) a single port biopsy and in 91 cases (54%) a tree port pars plana vitrectomy was performed. In 94% of the cases, sufficient Material was found for establishing the diagnosis. In 18%, a Cytolyt® wash was necessary to prevent clotting of the tubes in the Cellient® due to the viscosity of the sample. In 21% the diagnosis was an acute inflammation (presence of granulocytes), in 32% chronic active inflammation (presence of T-lymphocytes), in 35% low-grade inflammation (presence of CD68 cells, without T-lymphocytes); and in 5% a malignant process. In 9% a granulomatous inflammation was detected, in 5% glial tissue (floaters). In 6% no diagnosis was found. Conclusion A standardized protocol for sampling and handling Vitreous biopsies, fixing in PreservCyt® and processing by the Cellient® system gives a superior result in morphology, number of cells, and possibility of immuno-histochemical stainings. The diagnosis can be established or confirmed in more than 90% of cases.
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a new and standardized method to sample and analyse Vitreous samples by the cellient automated cell block system
Acta Ophthalmologica, 2013Co-Authors: Rita Van Ginderdeuren, Joachim Van Calster, Peter Stalmans, Joost Van Den OordAbstract:Purpose In this prospective study, a universal protocol for sampling and analysing Vitreous Material was investigated. Vitreous biopsies are difficult to handle because of the paucity of cells and the gelatinous structure of the Vitreous. Histopathological analysis of the Vitreous is useful in difficult uveitis cases to differentiate uveitis from lymphoma or infection and to define the type of cellular reaction. Methods Hundred consecutive Vitreous samples were analysed with the Cellient® tissue processor (Hologic). This machine is a fully automated processor starting from a specified container with PreservCyt® (fixative fluid) with cells to paraffin. Cytology was compared with fixatives Cytolyt® (contains a mucolyticum) and PreservCyt®. Routine histochemical and immunostainings were evaluated. Results In 92% of the cases, sufficient Material was found for diagnosis. In 14%, a Cytolyt® wash was necessary to prevent clotting of the tubes in the Cellient® due to the viscosity of the sample. In 23%, the diagnosis was an acute inflammation (presence of granulocytes); in 33%, chronic active inflammation (presence of T lymphocytes); in 33%, low-grade inflammation (presence of CD68 cells, without T lymphocytes); and in 3%, a malignant process. Conclusion A standardized protocol for sampling and handling Vitreous biopsies, fixing in PreservCyt® and processing by the Cellient® gives a satisfactory result in morphology, number of cells and possibility of immuno-histochemical stainings. The diagnosis can be established or confirmed in more than 90% of cases.
Joost Van Den Oord - One of the best experts on this subject based on the ideXlab platform.
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a new and standardized method to sample and analyse Vitreous samples by the cellient automated cell block system
Acta Ophthalmologica, 2013Co-Authors: Rita Van Ginderdeuren, Joachim Van Calster, Peter Stalmans, Joost Van Den OordAbstract:Purpose In this prospective study, a universal protocol for sampling and analysing Vitreous Material was investigated. Vitreous biopsies are difficult to handle because of the paucity of cells and the gelatinous structure of the Vitreous. Histopathological analysis of the Vitreous is useful in difficult uveitis cases to differentiate uveitis from lymphoma or infection and to define the type of cellular reaction. Methods Hundred consecutive Vitreous samples were analysed with the Cellient® tissue processor (Hologic). This machine is a fully automated processor starting from a specified container with PreservCyt® (fixative fluid) with cells to paraffin. Cytology was compared with fixatives Cytolyt® (contains a mucolyticum) and PreservCyt®. Routine histochemical and immunostainings were evaluated. Results In 92% of the cases, sufficient Material was found for diagnosis. In 14%, a Cytolyt® wash was necessary to prevent clotting of the tubes in the Cellient® due to the viscosity of the sample. In 23%, the diagnosis was an acute inflammation (presence of granulocytes); in 33%, chronic active inflammation (presence of T lymphocytes); in 33%, low-grade inflammation (presence of CD68 cells, without T lymphocytes); and in 3%, a malignant process. Conclusion A standardized protocol for sampling and handling Vitreous biopsies, fixing in PreservCyt® and processing by the Cellient® gives a satisfactory result in morphology, number of cells and possibility of immuno-histochemical stainings. The diagnosis can be established or confirmed in more than 90% of cases.
José Antonio Odriozola - One of the best experts on this subject based on the ideXlab platform.
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Recycling of construction and demolition waste generated by building infrastructure for the production of glassy Materials
Ceramics International, 2016Co-Authors: A. Domínguez, M I Dominguez, Miguel Ángel Centeno, S. Ivanova, José Antonio OdriozolaAbstract:The use of waste Materials generated by construction and demolition industry to yield valuable glassy Materials, i.e. enamel for glazed ceramic tiles and cellular glasses is presented in this study. Both types of Materials are produced by one-step treatment at moderate temperatures after simple waste chemical composition adjust. The enamels are manufactured directly from the initial waste powder by melting, while the expanded Materials result from mixing of the Vitreous Material obtained after waste vitrification with an adequate foaming agent and posterior thermal treatment. Through the manuscript the feasibility of one step production of second generation profit Materials is discussed in order to help achieving sustainable development and environmental protection.
Pontikes Yiannis - One of the best experts on this subject based on the ideXlab platform.
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INCREASING THE DIMENSIONAL STABILITY OF CAO-FEOX-AL2O3-SIO2 ALKALI-ACTIVATED MaterialS: ON THE SWELLING POTENTIAL OF CALCIUM OXIDE-RICH ADMIXTURES
'Eurowaste SRL', 2019Co-Authors: Marchi Maurizio, Segata Monica, Faleschini Flora, Pontikes YiannisAbstract:Advanced thermochemical conversion processes are emerging technologies for Materials\u2019 recovery and energetic conversion of wastes. During these processes, a (semi-)Vitreous Material is also produced, and as these technologies get closer to maturity and full-scale implementation, significant volumes of these secondary outputs are expected to be generated. The production of building Materials through the alkali activation of such residues is often identified as a possible large-scale valorization route, but the high susceptibility of alkali-activated Materials (AAM) to shrinkage limits their attractiveness to the construction sector. Aiming to mitigate such a phenomenon, an experimental study was conducted investigating the effect of calcium oxide-rich admixtures on the dimensional stability of CaO-FeOx-Al2O3-SiO2 AAMs. This work describes the impacts of such admixtures on autogenous and drying shrinkage, porosity, microstructure, and mineralogy on AAMs. Drying shrinkage was identified as the governing mechanism affecting AAM volumetric stability, whereas autogenous shrinkage was less significant. The reference pastes presented the highest drying shrinkage, while increasing the dosage of shrinkage reducing agent (SRA) was found to reduce drying shrinkage up to 63%. The reduction of drying shrinkage was proportional to SRA content; however, elevated dosages of such admixture were found to be detrimental for AAM microstructure. On the other hand, small dosages of calcium oxide-rich admixtures did not induce significant changes in the samples\u2019 mineralogical evolution but promoted the formation of denser and less fractured microstructures. The results presented here show that calcium oxide-rich admixtures can be used to increase AAM\u2019s volumetric stability and an optimal dosage is prescribed
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Increasing the dimensional stability of CaO-FeOx-Al₂O₃-SiO₂ alkali-activated Materials: On the swelling potential of calcium oxide-rich admixtures
'Eurowaste SRL', 2019Co-Authors: Ascensao Guilherme, Segata Monica, Faleschini Flora, March Maurizio, Pontikes YiannisAbstract:Advanced thermochemical conversion processes are emerging technologies for Materials’ recovery and energetic conversion of wastes. During these processes, a (semi-)Vitreous Material is also produced, and as these technologies get closer to maturity and full-scale implementation, significant volumes of these secondary outputs are expected to be generated. The production of building Materials through the alkali activation of such residues is often identified as a possible large-scale valorization route, but the high susceptibility of alkali-activated Materials (AAM) to shrinkage limits their attractiveness to the construction sector. Aiming to mitigate such a phenomenon, an experimental study was conducted investigating the effect of calcium oxide-rich admixtures on the dimensional stability of CaO-FeOx-Al2O3-SiO2 AAMs. This work describes the impacts of such admixtures on autogenous and drying shrinkage, porosity, microstructure, and mineralogy on AAMs. Drying shrinkage was identified as the governing mechanism affecting AAM volumetric stability, whereas autogenous shrinkage was less significant. The reference pastes presented the highest drying shrinkage, while increasing the dosage of shrinkage reducing agent (SRA) was found to reduce drying shrinkage up to 63%. The reduction of drying shrinkage was proportional to SRA content; however, elevated dosages of such admixture were found to be detrimental for AAM microstructure. On the other hand, small dosages of calcium oxide-rich admixtures did not induce significant changes in the samples’ mineralogical evolution but promoted the formation of denser and less fractured microstructures. The results presented here show that calcium oxide-rich admixtures can be used to increase AAM’s volumetric stability and an optimal dosage is prescribed.status: publishe
