The Experts below are selected from a list of 552 Experts worldwide ranked by ideXlab platform
Rudolph C Johnson - One of the best experts on this subject based on the ideXlab platform.
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique ...
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique was characterized using the organophosphorus Nerve Agent VX. The limit of detection for VX in high-performance liquid chromatography (HPLC)-grade water, defined as the lowest calibrator concentration, was 25 pg/mL in a small, 500 μL sample. The method was characterized over the course of 22 sample sets containing calibrators, blanks, and quality control samples. Method precision, expressed as the mean relative standard deviation, was less than 9.2% for all calibrators. Quality control sample accuracy was 102% and 100% of the mean for VX spiked into HPLC-grade water at concentrations of 2.0 and 0.25 ng/mL, respectively. This method successfully was applied to aqueous extracts from soil, hamburger, and finished tap water spiked with VX. Recovery was 65%, 81%, and 100% from these matrixes, respectively. Biologically based extractions of organophosphorus compounds represent a new technique for sample extraction that provides an increase in extraction specificity and sensitivity.
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immunomagnetic separation and quantification of butyrylcholinesterase Nerve Agent adducts in human serum
Analytical Chemistry, 2010Co-Authors: Jennifer L S Sporty, Ronald A Evans, Edward M Jakubowski, Sharon W Lemire, Julie A Renner, Robert F Williams, Jurgen G Schmidt, Marcel J Van Der Schans, D Noort, Rudolph C JohnsonAbstract:A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring Nerve Agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 μL serum samples. The particle-antibody-BuChE product was rinsed and directly digested with pepsin. Native and isotopically enriched nonapeptides corresponding to the pepsin digest products for uninhibited BuChE, and sarin, cyclohexylsarin, VX, and Russian VX Nerve Agent-inhibited BuChE were synthesized for use as calibrators and internal standards, respectively. Internal standards were added to the filtered digest sample, and the samples were quantified via high performance liquid chromatography-isotope dilution-tandem mass spectrometry. The ratio of adducted to total BuChE nonapeptides was calculated for each Nerve Agent-exposed serum sample using data collected in a single chromatogram. Nerve Agent-inhibited quality control serum pools were characterized as part of method validation; the method was observed to have extremely low background noise. The measurement of both uninhibited and inhibited BuChE peptides compensated for any variations in the pepsin digestion before the internal standard peptide was added to the sample and may prove useful in individualizing patient results following a Nerve Agent exposure. © 2010 American Chemical Society.
Ronald A Evans - One of the best experts on this subject based on the ideXlab platform.
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique ...
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique was characterized using the organophosphorus Nerve Agent VX. The limit of detection for VX in high-performance liquid chromatography (HPLC)-grade water, defined as the lowest calibrator concentration, was 25 pg/mL in a small, 500 μL sample. The method was characterized over the course of 22 sample sets containing calibrators, blanks, and quality control samples. Method precision, expressed as the mean relative standard deviation, was less than 9.2% for all calibrators. Quality control sample accuracy was 102% and 100% of the mean for VX spiked into HPLC-grade water at concentrations of 2.0 and 0.25 ng/mL, respectively. This method successfully was applied to aqueous extracts from soil, hamburger, and finished tap water spiked with VX. Recovery was 65%, 81%, and 100% from these matrixes, respectively. Biologically based extractions of organophosphorus compounds represent a new technique for sample extraction that provides an increase in extraction specificity and sensitivity.
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immunomagnetic separation and quantification of butyrylcholinesterase Nerve Agent adducts in human serum
Analytical Chemistry, 2010Co-Authors: Jennifer L S Sporty, Ronald A Evans, Edward M Jakubowski, Sharon W Lemire, Julie A Renner, Robert F Williams, Jurgen G Schmidt, Marcel J Van Der Schans, D Noort, Rudolph C JohnsonAbstract:A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring Nerve Agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 μL serum samples. The particle-antibody-BuChE product was rinsed and directly digested with pepsin. Native and isotopically enriched nonapeptides corresponding to the pepsin digest products for uninhibited BuChE, and sarin, cyclohexylsarin, VX, and Russian VX Nerve Agent-inhibited BuChE were synthesized for use as calibrators and internal standards, respectively. Internal standards were added to the filtered digest sample, and the samples were quantified via high performance liquid chromatography-isotope dilution-tandem mass spectrometry. The ratio of adducted to total BuChE nonapeptides was calculated for each Nerve Agent-exposed serum sample using data collected in a single chromatogram. Nerve Agent-inhibited quality control serum pools were characterized as part of method validation; the method was observed to have extremely low background noise. The measurement of both uninhibited and inhibited BuChE peptides compensated for any variations in the pepsin digestion before the internal standard peptide was added to the sample and may prove useful in individualizing patient results following a Nerve Agent exposure. © 2010 American Chemical Society.
Edward M Jakubowski - One of the best experts on this subject based on the ideXlab platform.
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique ...
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique was characterized using the organophosphorus Nerve Agent VX. The limit of detection for VX in high-performance liquid chromatography (HPLC)-grade water, defined as the lowest calibrator concentration, was 25 pg/mL in a small, 500 μL sample. The method was characterized over the course of 22 sample sets containing calibrators, blanks, and quality control samples. Method precision, expressed as the mean relative standard deviation, was less than 9.2% for all calibrators. Quality control sample accuracy was 102% and 100% of the mean for VX spiked into HPLC-grade water at concentrations of 2.0 and 0.25 ng/mL, respectively. This method successfully was applied to aqueous extracts from soil, hamburger, and finished tap water spiked with VX. Recovery was 65%, 81%, and 100% from these matrixes, respectively. Biologically based extractions of organophosphorus compounds represent a new technique for sample extraction that provides an increase in extraction specificity and sensitivity.
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immunomagnetic separation and quantification of butyrylcholinesterase Nerve Agent adducts in human serum
Analytical Chemistry, 2010Co-Authors: Jennifer L S Sporty, Ronald A Evans, Edward M Jakubowski, Sharon W Lemire, Julie A Renner, Robert F Williams, Jurgen G Schmidt, Marcel J Van Der Schans, D Noort, Rudolph C JohnsonAbstract:A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring Nerve Agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 μL serum samples. The particle-antibody-BuChE product was rinsed and directly digested with pepsin. Native and isotopically enriched nonapeptides corresponding to the pepsin digest products for uninhibited BuChE, and sarin, cyclohexylsarin, VX, and Russian VX Nerve Agent-inhibited BuChE were synthesized for use as calibrators and internal standards, respectively. Internal standards were added to the filtered digest sample, and the samples were quantified via high performance liquid chromatography-isotope dilution-tandem mass spectrometry. The ratio of adducted to total BuChE nonapeptides was calculated for each Nerve Agent-exposed serum sample using data collected in a single chromatogram. Nerve Agent-inhibited quality control serum pools were characterized as part of method validation; the method was observed to have extremely low background noise. The measurement of both uninhibited and inhibited BuChE peptides compensated for any variations in the pepsin digestion before the internal standard peptide was added to the sample and may prove useful in individualizing patient results following a Nerve Agent exposure. © 2010 American Chemical Society.
Jennifer S Knaack - One of the best experts on this subject based on the ideXlab platform.
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique ...
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique was characterized using the organophosphorus Nerve Agent VX. The limit of detection for VX in high-performance liquid chromatography (HPLC)-grade water, defined as the lowest calibrator concentration, was 25 pg/mL in a small, 500 μL sample. The method was characterized over the course of 22 sample sets containing calibrators, blanks, and quality control samples. Method precision, expressed as the mean relative standard deviation, was less than 9.2% for all calibrators. Quality control sample accuracy was 102% and 100% of the mean for VX spiked into HPLC-grade water at concentrations of 2.0 and 0.25 ng/mL, respectively. This method successfully was applied to aqueous extracts from soil, hamburger, and finished tap water spiked with VX. Recovery was 65%, 81%, and 100% from these matrixes, respectively. Biologically based extractions of organophosphorus compounds represent a new technique for sample extraction that provides an increase in extraction specificity and sensitivity.
Yingtao Zhou - One of the best experts on this subject based on the ideXlab platform.
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique ...
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high throughput immunomagnetic scavenging technique for quantitative analysis of live VX Nerve Agent in water hamburger and soil matrixes
Analytical Chemistry, 2012Co-Authors: Jennifer S Knaack, Yingtao Zhou, Carter W Abney, Samantha M Prezioso, Matthew L Magnuson, Ronald A Evans, Edward M Jakubowski, Katelyn Hardy, Rudolph C JohnsonAbstract:We have developed a novel immunomagnetic scavenging technique for extracting cholinesterase inhibitors from aqueous matrixes using biological targeting and antibody-based extraction. The technique was characterized using the organophosphorus Nerve Agent VX. The limit of detection for VX in high-performance liquid chromatography (HPLC)-grade water, defined as the lowest calibrator concentration, was 25 pg/mL in a small, 500 μL sample. The method was characterized over the course of 22 sample sets containing calibrators, blanks, and quality control samples. Method precision, expressed as the mean relative standard deviation, was less than 9.2% for all calibrators. Quality control sample accuracy was 102% and 100% of the mean for VX spiked into HPLC-grade water at concentrations of 2.0 and 0.25 ng/mL, respectively. This method successfully was applied to aqueous extracts from soil, hamburger, and finished tap water spiked with VX. Recovery was 65%, 81%, and 100% from these matrixes, respectively. Biologically based extractions of organophosphorus compounds represent a new technique for sample extraction that provides an increase in extraction specificity and sensitivity.
