The Experts below are selected from a list of 959874 Experts worldwide ranked by ideXlab platform
E Nordborg - One of the best experts on this subject based on the ideXlab platform.
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A Western Blot and molecular genetic investigation of the estrogen receptor beta in giant cell arteritis
Clinical and Experimental Rheumatology, 2006Co-Authors: K Larsson, C Nordborg, A-r Moslemi, E NordborgAbstract:OBJECTIVE The epidemiology of giant cell arteritis (GCA) may indicate a pathogenetic relationship between GCA and female sex hormone metabolism; GCA is two to four times more common in women compared with men. Our previous analyses gave no support for the hypothesis that the pathogenesis of GCA should be related to somatic mutations in the estrogen receptor alpha (ERalpha) gene. The object of the present study was to investigate the size of the estrogen receptor beta (ERBeta), and the size and nucleotide sequence of the ERBeta gene in temporal arteries in GCA. METHODS The ERBeta protein was analyzed by Western Blot technique and the ERBeta gene by RT-PCR and direct sequencing of the PCR product. RESULTS Western Blot analysis revealed an ERBeta of normal size. There were no aberrations in size or nucleotide sequence in the ERBeta gene in the GCA patients. CONCLUSION The present observations gave no support for the hypothesis that somatic mutations in the ERBeta gene should be involved in the pathogenesis of GCA.
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A Western Blot and molecular genetic investigation of the estrogen receptor beta in giant cell arteritis.
Clinical and experimental rheumatology, 2006Co-Authors: K Larsson, C Nordborg, A-r Moslemi, E NordborgAbstract:The epidemiology of giant cell arteritis (GCA) may indicate a pathogenetic relationship between GCA and female sex hormone metabolism; GCA is two to four times more common in women compared with men. Our previous analyses gave no support for the hypothesis that the pathogenesis of GCA should be related to somatic mutations in the estrogen receptor alpha (ERalpha) gene. The object of the present study was to investigate the size of the estrogen receptor beta (ERBeta), and the size and nucleotide sequence of the ERBeta gene in temporal arteries in GCA. The ERBeta protein was analyzed by Western Blot technique and the ERBeta gene by RT-PCR and direct sequencing of the PCR product. Western Blot analysis revealed an ERBeta of normal size. There were no aberrations in size or nucleotide sequence in the ERBeta gene in the GCA patients. The present observations gave no support for the hypothesis that somatic mutations in the ERBeta gene should be involved in the pathogenesis of GCA.
Daniel J Macphee - One of the best experts on this subject based on the ideXlab platform.
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Methodological considerations for improving Western Blot analysis.
Journal of Pharmacological and Toxicological Methods, 2009Co-Authors: Daniel J MacpheeAbstract:The need for a technique that could allow the determination of antigen specificity of antisera led to the development of a method that allowed the production of a replica of proteins, which had been separated electrophoretically on polyacrylamide gels, on to a nitrocellulose membrane. This method was coined Western Blotting and is very useful to study the presence, relative abundance, relative molecular mass, post-translational modification, and interaction of specific proteins. As a result it is utilized routinely in many fields of scientific research such as chemistry, biology and biomedical sciences. This review serves to touch on some of the methodological conditions that should be considered to improve Western Blot analysis, particularly as a guide for graduate students but also scientists who wish to continue adapting this now fundamental research tool.
K Larsson - One of the best experts on this subject based on the ideXlab platform.
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A Western Blot and molecular genetic investigation of the estrogen receptor beta in giant cell arteritis
Clinical and Experimental Rheumatology, 2006Co-Authors: K Larsson, C Nordborg, A-r Moslemi, E NordborgAbstract:OBJECTIVE The epidemiology of giant cell arteritis (GCA) may indicate a pathogenetic relationship between GCA and female sex hormone metabolism; GCA is two to four times more common in women compared with men. Our previous analyses gave no support for the hypothesis that the pathogenesis of GCA should be related to somatic mutations in the estrogen receptor alpha (ERalpha) gene. The object of the present study was to investigate the size of the estrogen receptor beta (ERBeta), and the size and nucleotide sequence of the ERBeta gene in temporal arteries in GCA. METHODS The ERBeta protein was analyzed by Western Blot technique and the ERBeta gene by RT-PCR and direct sequencing of the PCR product. RESULTS Western Blot analysis revealed an ERBeta of normal size. There were no aberrations in size or nucleotide sequence in the ERBeta gene in the GCA patients. CONCLUSION The present observations gave no support for the hypothesis that somatic mutations in the ERBeta gene should be involved in the pathogenesis of GCA.
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A Western Blot and molecular genetic investigation of the estrogen receptor beta in giant cell arteritis.
Clinical and experimental rheumatology, 2006Co-Authors: K Larsson, C Nordborg, A-r Moslemi, E NordborgAbstract:The epidemiology of giant cell arteritis (GCA) may indicate a pathogenetic relationship between GCA and female sex hormone metabolism; GCA is two to four times more common in women compared with men. Our previous analyses gave no support for the hypothesis that the pathogenesis of GCA should be related to somatic mutations in the estrogen receptor alpha (ERalpha) gene. The object of the present study was to investigate the size of the estrogen receptor beta (ERBeta), and the size and nucleotide sequence of the ERBeta gene in temporal arteries in GCA. The ERBeta protein was analyzed by Western Blot technique and the ERBeta gene by RT-PCR and direct sequencing of the PCR product. Western Blot analysis revealed an ERBeta of normal size. There were no aberrations in size or nucleotide sequence in the ERBeta gene in the GCA patients. The present observations gave no support for the hypothesis that somatic mutations in the ERBeta gene should be involved in the pathogenesis of GCA.
Qi Yan - One of the best experts on this subject based on the ideXlab platform.
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Evaluation on the Cambridge Biotech HIV-1 Western Blot kit
Chinese journal of epidemiology, 2006Co-Authors: Qi YanAbstract:Objective To evaluate the Cambridge Biotech HIV-1 Western Blot kit and to assay the Sensitivity and Specificity in the different HIV infected status groups in the fields. Methods To collect the serum specimens from 645 cases, using Cambridge Biotech HIV-1 Western Blot kit and using Genelabs Diagnostics HIV Blot 2.2 Western Blot kit to confirm the presence of HIV antibody through ELISA test. Results On HIV antibody positive individuals, Cambridge Biotech HIV-1 Western Blot kit and Genelabs Diagnostics HIV Blot 2.2 Western Blot kit showed positive reaction. The sensitivity of both Western Blot kits was 100% . Of 398 serum specimens from ELISA testing on HIV antibody negative individuals, 23 showed indeterminate when tested by Cambridge Biotech HIV-1 Western Blot kit and 86 showed indeterminate when tested by Genelabs Diagnostics HIV Blot 2.2 Western Blot kit. In such HIV screen test negative groups, the rates of specificity of Cambridge kit were 94.22% and 78.39% for Genelabs kit respectively. Conclusion The Cambridge Biotech HIV-1 Western Blot kit had much higher specificity then Genelabs Diagnostics HIV Blot 2.2 Western Blot kit.
C Nordborg - One of the best experts on this subject based on the ideXlab platform.
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A Western Blot and molecular genetic investigation of the estrogen receptor beta in giant cell arteritis
Clinical and Experimental Rheumatology, 2006Co-Authors: K Larsson, C Nordborg, A-r Moslemi, E NordborgAbstract:OBJECTIVE The epidemiology of giant cell arteritis (GCA) may indicate a pathogenetic relationship between GCA and female sex hormone metabolism; GCA is two to four times more common in women compared with men. Our previous analyses gave no support for the hypothesis that the pathogenesis of GCA should be related to somatic mutations in the estrogen receptor alpha (ERalpha) gene. The object of the present study was to investigate the size of the estrogen receptor beta (ERBeta), and the size and nucleotide sequence of the ERBeta gene in temporal arteries in GCA. METHODS The ERBeta protein was analyzed by Western Blot technique and the ERBeta gene by RT-PCR and direct sequencing of the PCR product. RESULTS Western Blot analysis revealed an ERBeta of normal size. There were no aberrations in size or nucleotide sequence in the ERBeta gene in the GCA patients. CONCLUSION The present observations gave no support for the hypothesis that somatic mutations in the ERBeta gene should be involved in the pathogenesis of GCA.
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A Western Blot and molecular genetic investigation of the estrogen receptor beta in giant cell arteritis.
Clinical and experimental rheumatology, 2006Co-Authors: K Larsson, C Nordborg, A-r Moslemi, E NordborgAbstract:The epidemiology of giant cell arteritis (GCA) may indicate a pathogenetic relationship between GCA and female sex hormone metabolism; GCA is two to four times more common in women compared with men. Our previous analyses gave no support for the hypothesis that the pathogenesis of GCA should be related to somatic mutations in the estrogen receptor alpha (ERalpha) gene. The object of the present study was to investigate the size of the estrogen receptor beta (ERBeta), and the size and nucleotide sequence of the ERBeta gene in temporal arteries in GCA. The ERBeta protein was analyzed by Western Blot technique and the ERBeta gene by RT-PCR and direct sequencing of the PCR product. Western Blot analysis revealed an ERBeta of normal size. There were no aberrations in size or nucleotide sequence in the ERBeta gene in the GCA patients. The present observations gave no support for the hypothesis that somatic mutations in the ERBeta gene should be involved in the pathogenesis of GCA.
