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Zofia Piotrowska-seget - One of the best experts on this subject based on the ideXlab platform.
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Response of rhizospheric and endophytic bacterial communities of White Mustard (Sinapis alba) to bioaugmentation of soil with the Pseudomonas sp. H15 strain.
Ecotoxicology and environmental safety, 2020Co-Authors: Tomasz Płociniczak, Magdalena Pacwa-płociniczak, Mirosław Kwaśniewski, Karolina Chwialkowska, Zofia Piotrowska-segetAbstract:Abstract A factor that may significantly increase the efficacy of phytoextraction is soil bioaugmentation with specific bacteria, which can alter the composition of rhizospheric and endophytic bacterial communities. The aim of this study was to compare the effect of soil treatment with living (bioaugmentation) and dead (control) cells of the plant growth-promoting metal-resistant endophytic strain Pseudomonas sp. H15 on the bacterial community composition in the rhizo- and endo-sphere of White Mustard during enhanced phytoextraction. The bacterial communities in the rhizosphere were dominated (51.7–68.2%) by Proteobacteria, regardless of the soil treatment or sampling point. A temporary increase in the number of sequences belonging to Gammaproteobacteria (up to 37.3%) was only observed 24 h after the soil treatment with living Pseudomonas sp. H15 cells, whereas for the remaining samples, the relative abundance of this class did not exceed 7.1%. The relative abundance of Proteobacteria in the endosphere of the roots, stems, and leaves of White Mustard was higher in the control than in bioaugmented plants. The most pronounced dominance of the Gammaproteobacteria sequences was observed in the stems and leaves of the control plants at the first sampling point, which strongly indicates the ability of the plants to rapidly uptake DNA from soil and translocate it to the aboveground parts of the plants. Additionally, the bioaugmentation of the soil caused a diverse shift in the bacterial communities in the rhizo- and endo-sphere of White Mustard compared to control. The most distinct differences, which were dependent on the treatment, were observed in the endosphere of plants at the beginning of the experiment and decreased over time. These results indicate that the rhizo- and endo-biome of White Mustard reacts to soil bioaugmentation and may influence the efficiency of bacterial-assisted phytoextraction.
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Rhizospheric Bacterial Strain Brevibacterium casei MH8a Colonizes Plant Tissues and Enhances Cd, Zn, Cu Phytoextraction by White Mustard
Frontiers in plant science, 2016Co-Authors: Tomasz Płociniczak, Aki Sinkkonen, Martin Romantschuk, Sławomir Sułowicz, Zofia Piotrowska-segetAbstract:Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants. The aim of this study was to determine whether the multiple-tolerant bacterium, Brevibacterium casei MH8a isolated from the heavy metal-contaminated rhizosphere soil of Sinapis alba L., is able to promote plant growth and enhance Cd, Zn and Cu uptake by White Mustard under laboratory conditions. Additionally, the ability of the rifampicin-resistant spontaneous mutant of MH8a to colonize plant tissues and its mechanisms of plant growth promotion were also examined. In order to assess the ecological consequences of bioaugmentation on autochthonous bacteria, the phospholipid fatty acid (PLFA) analysis was used. The MH8a strain exhibited the ability to produce ammonia, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and HCN but was not able to solubilize inorganic phosphate and produce siderophores. Introduction of MH8a into soil significantly increased S. alba biomass and the accumulation of Cd (208%), Zn (86%) and Cu (39%) in plant shoots in comparison with those grown in non-inoculated soil. Introduced into the soil, MH8a was able to enter the plant and was found in the roots and leaves of inoculated plants thus indicating its endophytic features. PLFA analysis revealed that the MH8a that was introduced into soil had a temporary influence on the structure of the autochthonous bacterial communities. The plant growth-promoting features of the MH8a strain and its ability to enhance the metal uptake by White Mustard and its long-term survival in soil as well as its temporary impact on autochthonous microorganisms make the strain a suitable candidate for the promotion of plant growth and the efficiency of phytoextraction.
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GFP-tagged multimetal-tolerant bacteria and their detection in the rhizosphere of White Mustard
Annals of microbiology, 2011Co-Authors: Zofia Piotrowska-seget, Grażyna Beściak, Tytus Bernaś, Jacek KozdrójAbstract:The introduction of rhizobacteria that tolerate heavy metals is a promising approach to support plants involved in phytoextraction and phytostabilisation. In this study, soil of a metal-mine wasteland was analyzed for the presence of metal-tolerant bacterial isolates, and the tolerance patterns of the isolated strains for a number of heavy metals and antibiotics were compared. Several of the multimetal-tolerant strains were tagged with a broad host range reporter plasmid (i.e. pPROBE-NT) bearing a green fluorescent protein marker gene (gfp). Overall, the metal-tolerant isolates were predominately Gram-negative bacteria. Most of the strains showed a tolerance to five metals (Zn, Cu, Ni, Pb and Cd), but with differing tolerance patterns. From among the successfully tagged isolates, we used the transconjugant Pseudomonas putida G25 (pPROBE-NT) to inoculate White Mustard seedlings. Despite a significant decrease in transconjugant abundance in the rhizosphere, the gfp-tagged cells survived on the root surfaces at a level previously reported for root colonisers.
Rupert Hochegger - One of the best experts on this subject based on the ideXlab platform.
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Development and validation of a duplex real-time PCR method for the simultaneous detection of celery and White Mustard in food.
Food chemistry, 2013Co-Authors: Magdalena Fuchs, Margit Cichna-markl, Rupert HocheggerAbstract:Abstract The developed duplex real-time PCR method allows the simultaneous detection of traces of potentially allergenic White Mustard (Sinapis alba) and celery roots (Apium graveolens var. rapaceum), celery stalks (A. g. var. dulce) and leaf celery (A. g. var. secalinum). The duplex assay does not show any cross-reactivity with 64 different biological species, including various members of the Brassicaceae and Apiaceae family. In raw model sausages spiked with White Mustard and celery roots, the LOD was found to be 0.001% White Mustard and 0.005% celery. In model sausages brewed at 75–78 °C for 15 min the LOD was found to be 0.005% White Mustard and 0.005% celery. The duplex real-time PCR assay was applied to check if commercial food products are labelled in compliance with the legal regulations.
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Development and validation of a duplex real-time PCR method for the simultaneous detection of celery and White Mustard in food
Clinical and Translational Allergy, 2011Co-Authors: Margit Cichna-markl, Magdalena Fuchs, Rupert HocheggerAbstract:Celery (celery root: Apium graveolens var. Rapaceum; leaf celery: A. g. var. Secalinum; celery stalks: A. g. var. Dulce) and Mustard (White or yellow Mustard: Sinapis alba; black Mustard: Brassica nigra; brown or oriental Mustard: Brassica juncea) are frequently used as ingredients in sauces, spices, sausages and other meat-products as well as in convenience products. Within the European Union, the presence of potentially allergenic celery and Mustard in foodstuffs has to be declared according to the EU legislative 2007/68/EC. The aim of the present study was to develop and validate a duplex real-time PCR method allowing the simultaneous detection of traces of celery and White Mustard in food. Primers and TaqMan probes were designed for the Apium graveolens NADPH-dependent mannose-6-phosphate reductase mRNA as well as the Sinapis alba mRNA for MADS D protein. PCR was performed on the RotorGene RG-3000 from Corbett Life Sciences. With the optimized duplex assay DNA extracted from celery root, leaf celery and celery stalks as well as DNA from White Mustard was amplified. The assay did not show any cross-reactivity with more than 60 food matrices, among them important members of the plant families Apiaceae and Brassicaceae, spices and different meat species. The LOD in serially diluted DNA extracts from celery root, leaf celery, celery stalks and White Mustard was found to be 2 pg/μL (10 pg absolute). The PCR efficiency was 99.4% for celery root, 108.3% for celery stalks, 96.5% for leaf celery and 99.0% for White Mustard. The LOD in DNA extracts obtained by extraction of model sausages was 0.005% (50 mg/kg) for celery and 0.001% (10 mg/kg) for White Mustard, in both raw and brewed model sausages. The PCR efficiency was 90.0% (celery) and 101.1% (White Mustard) in raw model sausages and 85.8% (celery) and 91.2% (White Mustard) in brewed model sausages.
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Development and validation of a real-time PCR method for the detection of White Mustard (Sinapis alba) in foods.
Journal of agricultural and food chemistry, 2010Co-Authors: Magdalena Fuchs, Margit Cichna-markl, Rupert HocheggerAbstract:This paper presents a real-time PCR method allowing the detection of traces of White Mustard (Sinapis alba) in complex food matrices. The primers and the probe are targeted at the gene coding for S. alba MADS D. The real-time PCR method was found to be specific for White Mustard and did not show any cross-reactivity with 67 biological species, including 12 members of the Brassicaceae family. The limit of detection, determined by analyzing serially diluted White Mustard DNA extracts, was 1 pg of White Mustard DNA/μL, corresponding to 5 pg of White Mustard DNA. In model sausages, the limit of detection was found to be 0.001% White Mustard (corresponding to 10 ppm or 10 mg/kg). The real-time PCR method was applied to verify the correct declaration of 20 foodstuffs purchased from Austrian supermarkets. White Mustard DNA was detected in one of three samples labeled with “may contain traces of Mustard” and in one of seven samples without any information on the presence of Mustard.
Tomasz Płociniczak - One of the best experts on this subject based on the ideXlab platform.
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Response of rhizospheric and endophytic bacterial communities of White Mustard (Sinapis alba) to bioaugmentation of soil with the Pseudomonas sp. H15 strain.
Ecotoxicology and environmental safety, 2020Co-Authors: Tomasz Płociniczak, Magdalena Pacwa-płociniczak, Mirosław Kwaśniewski, Karolina Chwialkowska, Zofia Piotrowska-segetAbstract:Abstract A factor that may significantly increase the efficacy of phytoextraction is soil bioaugmentation with specific bacteria, which can alter the composition of rhizospheric and endophytic bacterial communities. The aim of this study was to compare the effect of soil treatment with living (bioaugmentation) and dead (control) cells of the plant growth-promoting metal-resistant endophytic strain Pseudomonas sp. H15 on the bacterial community composition in the rhizo- and endo-sphere of White Mustard during enhanced phytoextraction. The bacterial communities in the rhizosphere were dominated (51.7–68.2%) by Proteobacteria, regardless of the soil treatment or sampling point. A temporary increase in the number of sequences belonging to Gammaproteobacteria (up to 37.3%) was only observed 24 h after the soil treatment with living Pseudomonas sp. H15 cells, whereas for the remaining samples, the relative abundance of this class did not exceed 7.1%. The relative abundance of Proteobacteria in the endosphere of the roots, stems, and leaves of White Mustard was higher in the control than in bioaugmented plants. The most pronounced dominance of the Gammaproteobacteria sequences was observed in the stems and leaves of the control plants at the first sampling point, which strongly indicates the ability of the plants to rapidly uptake DNA from soil and translocate it to the aboveground parts of the plants. Additionally, the bioaugmentation of the soil caused a diverse shift in the bacterial communities in the rhizo- and endo-sphere of White Mustard compared to control. The most distinct differences, which were dependent on the treatment, were observed in the endosphere of plants at the beginning of the experiment and decreased over time. These results indicate that the rhizo- and endo-biome of White Mustard reacts to soil bioaugmentation and may influence the efficiency of bacterial-assisted phytoextraction.
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Rhizospheric Bacterial Strain Brevibacterium casei MH8a Colonizes Plant Tissues and Enhances Cd, Zn, Cu Phytoextraction by White Mustard
Frontiers in plant science, 2016Co-Authors: Tomasz Płociniczak, Aki Sinkkonen, Martin Romantschuk, Sławomir Sułowicz, Zofia Piotrowska-segetAbstract:Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants. The aim of this study was to determine whether the multiple-tolerant bacterium, Brevibacterium casei MH8a isolated from the heavy metal-contaminated rhizosphere soil of Sinapis alba L., is able to promote plant growth and enhance Cd, Zn and Cu uptake by White Mustard under laboratory conditions. Additionally, the ability of the rifampicin-resistant spontaneous mutant of MH8a to colonize plant tissues and its mechanisms of plant growth promotion were also examined. In order to assess the ecological consequences of bioaugmentation on autochthonous bacteria, the phospholipid fatty acid (PLFA) analysis was used. The MH8a strain exhibited the ability to produce ammonia, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and HCN but was not able to solubilize inorganic phosphate and produce siderophores. Introduction of MH8a into soil significantly increased S. alba biomass and the accumulation of Cd (208%), Zn (86%) and Cu (39%) in plant shoots in comparison with those grown in non-inoculated soil. Introduced into the soil, MH8a was able to enter the plant and was found in the roots and leaves of inoculated plants thus indicating its endophytic features. PLFA analysis revealed that the MH8a that was introduced into soil had a temporary influence on the structure of the autochthonous bacterial communities. The plant growth-promoting features of the MH8a strain and its ability to enhance the metal uptake by White Mustard and its long-term survival in soil as well as its temporary impact on autochthonous microorganisms make the strain a suitable candidate for the promotion of plant growth and the efficiency of phytoextraction.
Magdalena Fuchs - One of the best experts on this subject based on the ideXlab platform.
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Development and validation of a duplex real-time PCR method for the simultaneous detection of celery and White Mustard in food.
Food chemistry, 2013Co-Authors: Magdalena Fuchs, Margit Cichna-markl, Rupert HocheggerAbstract:Abstract The developed duplex real-time PCR method allows the simultaneous detection of traces of potentially allergenic White Mustard (Sinapis alba) and celery roots (Apium graveolens var. rapaceum), celery stalks (A. g. var. dulce) and leaf celery (A. g. var. secalinum). The duplex assay does not show any cross-reactivity with 64 different biological species, including various members of the Brassicaceae and Apiaceae family. In raw model sausages spiked with White Mustard and celery roots, the LOD was found to be 0.001% White Mustard and 0.005% celery. In model sausages brewed at 75–78 °C for 15 min the LOD was found to be 0.005% White Mustard and 0.005% celery. The duplex real-time PCR assay was applied to check if commercial food products are labelled in compliance with the legal regulations.
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Development and validation of a duplex real-time PCR method for the simultaneous detection of celery and White Mustard in food
Clinical and Translational Allergy, 2011Co-Authors: Margit Cichna-markl, Magdalena Fuchs, Rupert HocheggerAbstract:Celery (celery root: Apium graveolens var. Rapaceum; leaf celery: A. g. var. Secalinum; celery stalks: A. g. var. Dulce) and Mustard (White or yellow Mustard: Sinapis alba; black Mustard: Brassica nigra; brown or oriental Mustard: Brassica juncea) are frequently used as ingredients in sauces, spices, sausages and other meat-products as well as in convenience products. Within the European Union, the presence of potentially allergenic celery and Mustard in foodstuffs has to be declared according to the EU legislative 2007/68/EC. The aim of the present study was to develop and validate a duplex real-time PCR method allowing the simultaneous detection of traces of celery and White Mustard in food. Primers and TaqMan probes were designed for the Apium graveolens NADPH-dependent mannose-6-phosphate reductase mRNA as well as the Sinapis alba mRNA for MADS D protein. PCR was performed on the RotorGene RG-3000 from Corbett Life Sciences. With the optimized duplex assay DNA extracted from celery root, leaf celery and celery stalks as well as DNA from White Mustard was amplified. The assay did not show any cross-reactivity with more than 60 food matrices, among them important members of the plant families Apiaceae and Brassicaceae, spices and different meat species. The LOD in serially diluted DNA extracts from celery root, leaf celery, celery stalks and White Mustard was found to be 2 pg/μL (10 pg absolute). The PCR efficiency was 99.4% for celery root, 108.3% for celery stalks, 96.5% for leaf celery and 99.0% for White Mustard. The LOD in DNA extracts obtained by extraction of model sausages was 0.005% (50 mg/kg) for celery and 0.001% (10 mg/kg) for White Mustard, in both raw and brewed model sausages. The PCR efficiency was 90.0% (celery) and 101.1% (White Mustard) in raw model sausages and 85.8% (celery) and 91.2% (White Mustard) in brewed model sausages.
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Development and validation of a real-time PCR method for the detection of White Mustard (Sinapis alba) in foods.
Journal of agricultural and food chemistry, 2010Co-Authors: Magdalena Fuchs, Margit Cichna-markl, Rupert HocheggerAbstract:This paper presents a real-time PCR method allowing the detection of traces of White Mustard (Sinapis alba) in complex food matrices. The primers and the probe are targeted at the gene coding for S. alba MADS D. The real-time PCR method was found to be specific for White Mustard and did not show any cross-reactivity with 67 biological species, including 12 members of the Brassicaceae family. The limit of detection, determined by analyzing serially diluted White Mustard DNA extracts, was 1 pg of White Mustard DNA/μL, corresponding to 5 pg of White Mustard DNA. In model sausages, the limit of detection was found to be 0.001% White Mustard (corresponding to 10 ppm or 10 mg/kg). The real-time PCR method was applied to verify the correct declaration of 20 foodstuffs purchased from Austrian supermarkets. White Mustard DNA was detected in one of three samples labeled with “may contain traces of Mustard” and in one of seven samples without any information on the presence of Mustard.
Krzysztof Józef Jankowski - One of the best experts on this subject based on the ideXlab platform.
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Canola-quality White Mustard: Agronomic management and seed yield
Industrial Crops and Products, 2020Co-Authors: Krzysztof Józef Jankowski, Dariusz Załuski, Mateusz SokólskiAbstract:Abstract Modern White Mustard (Sinapis alba L.) cultivars have high concentrations of erucic acid and glucosinolates. The progress in the breeding and cultivation of oilseed crops has contributed to the development of canola-quality White Mustard, a variety without erucic acid and with low glucosinolate content, which was released for commercial production in 2012. The introduction of a new variety of White Mustard could require partial or complete modification of the relevant production technology. The agricultural requirements of new crop cultivars can be rapidly assessed in experiments with fractional factorial design. In 2016–2018, a field experiment with mixed 2m3k−1 factorial design, where five factors were tested at two and three levels, was carried out in the Agricultural Experiment Station in Balcyny in north-eastern Poland. Two White Mustard cultivars, including the traditional cultivar Radena and the canola-quality cultivar Warta, were sown on three dates: the optimal date (4–10 April), as well as 7 and 14 days past the optimal date. White Mustard cultivars were grown at three levels of agricultural inputs (0, 1, 2) with different rates of nitrogen (80, 120 and 160 kg ha-1), sulfur (0, 20 and 40 kg ha-1) and boron fertilizers (0, 150 and 300 g ha-1). The yields of the traditional cultivar exceeded those of the canola-quality cultivar by 0.53 Mg ha-1 on average. Both cultivars produced the highest yields when sown on the optimal date. Delayed sowing (by 7 and 14 days) contributed to the greatest decrease in yield (32 % and 42 %, respectively) in a dry year (2018). The traditional cultivar was more sensitive to delayed sowing than the canola-quality cultivar. In both analyzed cultivars, nitrogen fertilizer delivered yield-forming effects up to the rate of 80 kg ha-1 (in the dry year) or 120 kg ha-1 (in the year with above-average precipitation, 2016–2017). In both cultivars, a significant increase in seed yield was observed in response to 20 kg S ha-1. Foliar application of boron fertilizer did not increase White Mustard yields.
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Classification of the seeds of traditional and double‐low cultivars of White Mustard based on texture features
Journal of Food Process Engineering, 2019Co-Authors: Ewa Ropelewska, Krzysztof Józef JankowskiAbstract:The aim of this study was to develop classification models based on the texture features from the images acquired with a flatbed scanner to distinguish between the seeds of traditional (Radena) and double‐low (Warta) cultivars of White Mustard. Texture features were calculated in MaZda software. The classification models for RGB, Lab, and XYZ color space and individual color channels for distinguishing between the seeds of traditional and double‐low cultivars of White Mustard were built in the WEKA application with the use of selected Decision Trees (J48), Rules (J Rip), Bayes (Naive Bayes), Lazy (IBk), Meta (Multi Class Classifier), and Functions (FLDA) classifiers. The classification accuracy of the evaluated color models ranged from 77% (XYZ, IBk classifier) to 83% (RGB, JRip classifier). In an evaluation of individual color channels, classification accuracy was highest for channels R, L, and X, and it ranged from 85% for color channels L and X when the IBk classifier was used to 93% for the texture features from color channel R when the JRip classifier was used. PRACTICAL APPLICATIONS: The developed models support fast, cheap, nondestructive, and reliable discrimination of the seeds of White Mustard. The proposed classification models based on texture features effectively distinguished between the seeds of traditional (Radena) and double‐low (Warta) cultivars of White Mustard. Therefore, they can be reliably used to examine the authenticity of seeds and to detect seed adulteration.
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classification of the seeds of traditional and double low cultivars of White Mustard based on texture features
Journal of Food Process Engineering, 2019Co-Authors: Ewa Ropelewska, Krzysztof Józef JankowskiAbstract:The aim of this study was to develop classification models based on the texture features from the images acquired with a flatbed scanner to distinguish between the seeds of traditional (Radena) and double‐low (Warta) cultivars of White Mustard. Texture features were calculated in MaZda software. The classification models for RGB, Lab, and XYZ color space and individual color channels for distinguishing between the seeds of traditional and double‐low cultivars of White Mustard were built in the WEKA application with the use of selected Decision Trees (J48), Rules (J Rip), Bayes (Naive Bayes), Lazy (IBk), Meta (Multi Class Classifier), and Functions (FLDA) classifiers. The classification accuracy of the evaluated color models ranged from 77% (XYZ, IBk classifier) to 83% (RGB, JRip classifier). In an evaluation of individual color channels, classification accuracy was highest for channels R, L, and X, and it ranged from 85% for color channels L and X when the IBk classifier was used to 93% for the texture features from color channel R when the JRip classifier was used. PRACTICAL APPLICATIONS: The developed models support fast, cheap, nondestructive, and reliable discrimination of the seeds of White Mustard. The proposed classification models based on texture features effectively distinguished between the seeds of traditional (Radena) and double‐low (Warta) cultivars of White Mustard. Therefore, they can be reliably used to examine the authenticity of seeds and to detect seed adulteration.
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Concentrations of copper, zinc and manganese in the roots, straw and oil cake of White Mustard (Sinapis alba L.) and Indian Mustard (Brassica juncea (L.) Czern. et Coss.) depending on sulphur fertilization
Plant Soil and Environment, 2018Co-Authors: Krzysztof Józef Jankowski, W. Budzynski, Łukasz Kijewski, A. KlasaAbstract:The purpose of this experiment was to determine the influence of the soil application of sulphur (S) on concentrations of micronutrients in the root residues, straw and oil cake of White and Indian Mustard. The plant material for chemical analyses originated from a controlled field experiment conducted in experimental fields at the University of Warmia and Mazury in Olsztyn, Poland (2006-2008). In both White and Indian Mustard, the richest source of Cu (7.2; 7.0 mg/kg dry matter (DM)) and Zn (64.6; 55.3 mg/kg DM) was the oil cake from Mustard seeds. Regarding Mn, both White and Indian Mustard accumulated the highest content of this element in roots (48.2; 50.8 mg/kg DM), less in oil cake (31.9; 35.5 mg/kg DM) and the least Mn was determined in straw of both species (24.0; 17.1 mg/kg DM). The application of sulphur caused a significant increase in the concentration of Zn and Mn in White Mustard roots. The content of micronutrients in roots of Indian Mustard was not differentiated significantly by S fertilization. The application of sulphur caused a significant decrease in the content of Mn in White Mustard straw and Cu in Indian Mustard straw. The content of micronutrients in White Mustard oil cake and Indian Mustard oil cake was not significantly changed by S fertilization.
