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José Ángel López-jiménez - One of the best experts on this subject based on the ideXlab platform.
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Effects of long-term ingestion of White Tea on oxidation produced by aging and acute oxidative damage in rats
Journal of Physiology and Biochemistry, 2017Co-Authors: Cristóbal Espinosa Ruiz, José Ángel López-jiménez, Salvador Zamora, Lorena Cabrera, Francisca Pérez-llamasAbstract:The infusion Tea extracted from the leaves of the plant Camellia sinensis can be used in the prevention of cancer, cardiovascular and neurodegenerative diseases, and aging, while adriamycin (ADR) is an anticancer drug that increases oxidative stress in cells. The present study evaluated the protective effect of the long-term consumption of White Tea used at two different doses against the oxidative stress produced by aging and acute oxidation caused ADR treatment. At wearing, rats received distilled water (control), or 0.15 (dose 1) or 0.45 mg (dose 2) of solid Tea extract/kilogram body weight in their drink. At 12 months, about half of the rats of each group were injected with a bolus of ADR, and six rats of the control group with an injection of saline solution and sacrificed. The rest of the animals continued in their cages until 24 months of age, when they were sacrificed. Lipid and protein oxidation of liver and brain microsomes was analyzed by measuring hydroperoxide and carbonyl levels. White Tea consumption for 12 months at a non-pharmacological dose was seen to reverse the oxidative damage caused by ADR in both liver and brain, while the consumption of White Tea for 20 months at a non-pharmacological dose had no effect on carbonyl or hydroperoxides in these tissues. The long-term ingestion of White Tea protected tissues from acute oxidative stress but did not affect chronic oxidative agents such aging.
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Long-term intake of White Tea prevents oxidative damage caused by adriamycin in kidney of rats.
Journal of the Science of Food and Agriculture, 2015Co-Authors: Cristóbal Espinosa, José Ángel López-jiménez, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador ZamoraAbstract:BACKGROUND White Tea infusion (Camelia sinensis) has antioxidants properties. The infusion contains polyphenols that have been proposed to induce antioxidant response element (ARE) response via nuclear factor E2-related factor 2 (NRF2). Adriamycin (ADR) has antitumour properties and oxidative effects. Oxidative stress is related to a variety of kidney diseases. Prevention of the oxidative stress through long-term intake of White Tea and the study of the molecular mechanisms involved in protection could be of great interest. Rats were given distilled water, 0.015 or 0.045 g of solid White Tea extract kg(-1) body weight for 12 months. Animals received an injection of ADR. In kidney, oxidative stress parameters were measured, the expressions of nuclear factor E2-related factor 2 gene (Nrf2), and detoxifying and antioxidants genes were analysed, and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured. RESULTS ADR administration increased oxidative parameters and decreased the antioxidant activity; significantly increased the expression of analysed genes and the activity of CAT and SOD and decreased GR activity. The highest White Tea dose protected redox status and inhibited ARE response. CONCLUSION Long-term intake of White Tea protected kidney against the oxidative stress. ADR activated the ARE response but in animals treated with the highest dose of White Tea, this response was inhibited, probably for antioxidant protection. © 2015 Society of Chemical Industry.
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Effect of long term intake of White Tea on acute oxidative stress in rats
Nutricion Hospitalaria, 2015Co-Authors: Cristóbal Espinosa, José Ángel López-jiménez, Francisca Pérez-llamas, Daniel González-silvera, Salvador ZamoraAbstract:Introduction: the well known antioxidant properties of White Tea include the prevention of cancer, neurodegenerative diseases and oxidative stress. Adriamycin can generate an amount of oxidative stress in vivo. Objective: evaluate long term intake of White Tea on plasma antioxidant capacity and on the fatty acid profile of liver and heart microsomes in animals subjected to acute oxidative stress. Methods: rats were given distilled water (controls), 15 mg/d (dose 1) or 45 mg/d (dose 2) of solid White Tea extract/per kilogram of body weight for 12 months. After this time, all the animals received an injection of adriamycin (ADR) (10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. Samples of plasma and liver and heart were taken. The antioxidant activity, the carbonyl groups and hydroperoxide concentration were analyzed in plasma, and the fatty acid profiles of liver and heart microsomes were obtained. Results & discussion: only the hydroperoxides showed significant changes, while slight tendencies were observed in antioxidant activity and the carbonyl groups. Although the long term intake of White Tea and the administration of adriamycin did not change the fatty acid profile, slight tendencies existed for the SFAs, MUFAs and PUFAs.
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Molecular mechanisms by which White Tea prevents oxidative stress
Journal of Physiology and Biochemistry, 2014Co-Authors: Cristóbal Espinosa, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador Zamora, José Ángel López-jiménezAbstract:The flavonoid content of Tea ( Camellia sinensis ) has beneficial properties in the prevention of diseases. However, the mechanisms by which White Tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid Tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 ( Nrf2 ), NAD(P)H:quinone oxidoreductase 1 ( Nqo1 ), glutathione S-transferase ( Gst ), haem oxygenase-1 ( Ho1 ), catalase ( Cat ), superoxide dismutase ( Sod ) and glutathione reductase ( Gr ) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2 , Gst , Nqo1 , Ho1 , Cat , Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of White Tea increased in a higher degree the expression of Nrf2 , Gst , Nqo1 and Ho1 in the Tea + ADR group compared with the control group and C + ADR group. In addition, Tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner.
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Molecular mechanisms by which White Tea prevents oxidative stress
Journal of Physiology and Biochemistry, 2014Co-Authors: Cristóbal Espinosa, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador Zamora, José Ángel López-jiménezAbstract:The flavonoid content of Tea ( Camellia sinensis ) has beneficial properties in the prevention of diseases. However, the mechanisms by which White Tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid Tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 ( Nrf2 ), NAD(P)H:quinone oxidoreductase 1 ( Nqo1 ), glutathione S-transferase ( Gst ), haem oxygenase-1 ( Ho1 ), catalase ( Cat ), superoxide dismutase ( Sod ) and glutathione reductase ( Gr ) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2 , Gst , Nqo1 , Ho1 , Cat , Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of White Tea increased in a higher degree the expression of Nrf2 , Gst , Nqo1 and Ho1 in the Tea + ADR group compared with the control group and C + ADR group. In addition, Tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner.
Salvador Zamora - One of the best experts on this subject based on the ideXlab platform.
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Effects of long-term ingestion of White Tea on oxidation produced by aging and acute oxidative damage in rats
Journal of Physiology and Biochemistry, 2017Co-Authors: Cristóbal Espinosa Ruiz, José Ángel López-jiménez, Salvador Zamora, Lorena Cabrera, Francisca Pérez-llamasAbstract:The infusion Tea extracted from the leaves of the plant Camellia sinensis can be used in the prevention of cancer, cardiovascular and neurodegenerative diseases, and aging, while adriamycin (ADR) is an anticancer drug that increases oxidative stress in cells. The present study evaluated the protective effect of the long-term consumption of White Tea used at two different doses against the oxidative stress produced by aging and acute oxidation caused ADR treatment. At wearing, rats received distilled water (control), or 0.15 (dose 1) or 0.45 mg (dose 2) of solid Tea extract/kilogram body weight in their drink. At 12 months, about half of the rats of each group were injected with a bolus of ADR, and six rats of the control group with an injection of saline solution and sacrificed. The rest of the animals continued in their cages until 24 months of age, when they were sacrificed. Lipid and protein oxidation of liver and brain microsomes was analyzed by measuring hydroperoxide and carbonyl levels. White Tea consumption for 12 months at a non-pharmacological dose was seen to reverse the oxidative damage caused by ADR in both liver and brain, while the consumption of White Tea for 20 months at a non-pharmacological dose had no effect on carbonyl or hydroperoxides in these tissues. The long-term ingestion of White Tea protected tissues from acute oxidative stress but did not affect chronic oxidative agents such aging.
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Long-term intake of White Tea prevents oxidative damage caused by adriamycin in kidney of rats.
Journal of the Science of Food and Agriculture, 2015Co-Authors: Cristóbal Espinosa, José Ángel López-jiménez, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador ZamoraAbstract:BACKGROUND White Tea infusion (Camelia sinensis) has antioxidants properties. The infusion contains polyphenols that have been proposed to induce antioxidant response element (ARE) response via nuclear factor E2-related factor 2 (NRF2). Adriamycin (ADR) has antitumour properties and oxidative effects. Oxidative stress is related to a variety of kidney diseases. Prevention of the oxidative stress through long-term intake of White Tea and the study of the molecular mechanisms involved in protection could be of great interest. Rats were given distilled water, 0.015 or 0.045 g of solid White Tea extract kg(-1) body weight for 12 months. Animals received an injection of ADR. In kidney, oxidative stress parameters were measured, the expressions of nuclear factor E2-related factor 2 gene (Nrf2), and detoxifying and antioxidants genes were analysed, and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured. RESULTS ADR administration increased oxidative parameters and decreased the antioxidant activity; significantly increased the expression of analysed genes and the activity of CAT and SOD and decreased GR activity. The highest White Tea dose protected redox status and inhibited ARE response. CONCLUSION Long-term intake of White Tea protected kidney against the oxidative stress. ADR activated the ARE response but in animals treated with the highest dose of White Tea, this response was inhibited, probably for antioxidant protection. © 2015 Society of Chemical Industry.
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Effect of long term intake of White Tea on acute oxidative stress in rats
Nutricion Hospitalaria, 2015Co-Authors: Cristóbal Espinosa, José Ángel López-jiménez, Francisca Pérez-llamas, Daniel González-silvera, Salvador ZamoraAbstract:Introduction: the well known antioxidant properties of White Tea include the prevention of cancer, neurodegenerative diseases and oxidative stress. Adriamycin can generate an amount of oxidative stress in vivo. Objective: evaluate long term intake of White Tea on plasma antioxidant capacity and on the fatty acid profile of liver and heart microsomes in animals subjected to acute oxidative stress. Methods: rats were given distilled water (controls), 15 mg/d (dose 1) or 45 mg/d (dose 2) of solid White Tea extract/per kilogram of body weight for 12 months. After this time, all the animals received an injection of adriamycin (ADR) (10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. Samples of plasma and liver and heart were taken. The antioxidant activity, the carbonyl groups and hydroperoxide concentration were analyzed in plasma, and the fatty acid profiles of liver and heart microsomes were obtained. Results & discussion: only the hydroperoxides showed significant changes, while slight tendencies were observed in antioxidant activity and the carbonyl groups. Although the long term intake of White Tea and the administration of adriamycin did not change the fatty acid profile, slight tendencies existed for the SFAs, MUFAs and PUFAs.
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Molecular mechanisms by which White Tea prevents oxidative stress
Journal of Physiology and Biochemistry, 2014Co-Authors: Cristóbal Espinosa, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador Zamora, José Ángel López-jiménezAbstract:The flavonoid content of Tea ( Camellia sinensis ) has beneficial properties in the prevention of diseases. However, the mechanisms by which White Tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid Tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 ( Nrf2 ), NAD(P)H:quinone oxidoreductase 1 ( Nqo1 ), glutathione S-transferase ( Gst ), haem oxygenase-1 ( Ho1 ), catalase ( Cat ), superoxide dismutase ( Sod ) and glutathione reductase ( Gr ) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2 , Gst , Nqo1 , Ho1 , Cat , Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of White Tea increased in a higher degree the expression of Nrf2 , Gst , Nqo1 and Ho1 in the Tea + ADR group compared with the control group and C + ADR group. In addition, Tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner.
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Molecular mechanisms by which White Tea prevents oxidative stress
Journal of Physiology and Biochemistry, 2014Co-Authors: Cristóbal Espinosa, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador Zamora, José Ángel López-jiménezAbstract:The flavonoid content of Tea ( Camellia sinensis ) has beneficial properties in the prevention of diseases. However, the mechanisms by which White Tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid Tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 ( Nrf2 ), NAD(P)H:quinone oxidoreductase 1 ( Nqo1 ), glutathione S-transferase ( Gst ), haem oxygenase-1 ( Ho1 ), catalase ( Cat ), superoxide dismutase ( Sod ) and glutathione reductase ( Gr ) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2 , Gst , Nqo1 , Ho1 , Cat , Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of White Tea increased in a higher degree the expression of Nrf2 , Gst , Nqo1 and Ho1 in the Tea + ADR group compared with the control group and C + ADR group. In addition, Tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner.
Francisca Pérez-llamas - One of the best experts on this subject based on the ideXlab platform.
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Effects of long-term ingestion of White Tea on oxidation produced by aging and acute oxidative damage in rats
Journal of Physiology and Biochemistry, 2017Co-Authors: Cristóbal Espinosa Ruiz, José Ángel López-jiménez, Salvador Zamora, Lorena Cabrera, Francisca Pérez-llamasAbstract:The infusion Tea extracted from the leaves of the plant Camellia sinensis can be used in the prevention of cancer, cardiovascular and neurodegenerative diseases, and aging, while adriamycin (ADR) is an anticancer drug that increases oxidative stress in cells. The present study evaluated the protective effect of the long-term consumption of White Tea used at two different doses against the oxidative stress produced by aging and acute oxidation caused ADR treatment. At wearing, rats received distilled water (control), or 0.15 (dose 1) or 0.45 mg (dose 2) of solid Tea extract/kilogram body weight in their drink. At 12 months, about half of the rats of each group were injected with a bolus of ADR, and six rats of the control group with an injection of saline solution and sacrificed. The rest of the animals continued in their cages until 24 months of age, when they were sacrificed. Lipid and protein oxidation of liver and brain microsomes was analyzed by measuring hydroperoxide and carbonyl levels. White Tea consumption for 12 months at a non-pharmacological dose was seen to reverse the oxidative damage caused by ADR in both liver and brain, while the consumption of White Tea for 20 months at a non-pharmacological dose had no effect on carbonyl or hydroperoxides in these tissues. The long-term ingestion of White Tea protected tissues from acute oxidative stress but did not affect chronic oxidative agents such aging.
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Long-term intake of White Tea prevents oxidative damage caused by adriamycin in kidney of rats.
Journal of the Science of Food and Agriculture, 2015Co-Authors: Cristóbal Espinosa, José Ángel López-jiménez, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador ZamoraAbstract:BACKGROUND White Tea infusion (Camelia sinensis) has antioxidants properties. The infusion contains polyphenols that have been proposed to induce antioxidant response element (ARE) response via nuclear factor E2-related factor 2 (NRF2). Adriamycin (ADR) has antitumour properties and oxidative effects. Oxidative stress is related to a variety of kidney diseases. Prevention of the oxidative stress through long-term intake of White Tea and the study of the molecular mechanisms involved in protection could be of great interest. Rats were given distilled water, 0.015 or 0.045 g of solid White Tea extract kg(-1) body weight for 12 months. Animals received an injection of ADR. In kidney, oxidative stress parameters were measured, the expressions of nuclear factor E2-related factor 2 gene (Nrf2), and detoxifying and antioxidants genes were analysed, and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured. RESULTS ADR administration increased oxidative parameters and decreased the antioxidant activity; significantly increased the expression of analysed genes and the activity of CAT and SOD and decreased GR activity. The highest White Tea dose protected redox status and inhibited ARE response. CONCLUSION Long-term intake of White Tea protected kidney against the oxidative stress. ADR activated the ARE response but in animals treated with the highest dose of White Tea, this response was inhibited, probably for antioxidant protection. © 2015 Society of Chemical Industry.
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Effect of long term intake of White Tea on acute oxidative stress in rats
Nutricion Hospitalaria, 2015Co-Authors: Cristóbal Espinosa, José Ángel López-jiménez, Francisca Pérez-llamas, Daniel González-silvera, Salvador ZamoraAbstract:Introduction: the well known antioxidant properties of White Tea include the prevention of cancer, neurodegenerative diseases and oxidative stress. Adriamycin can generate an amount of oxidative stress in vivo. Objective: evaluate long term intake of White Tea on plasma antioxidant capacity and on the fatty acid profile of liver and heart microsomes in animals subjected to acute oxidative stress. Methods: rats were given distilled water (controls), 15 mg/d (dose 1) or 45 mg/d (dose 2) of solid White Tea extract/per kilogram of body weight for 12 months. After this time, all the animals received an injection of adriamycin (ADR) (10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. Samples of plasma and liver and heart were taken. The antioxidant activity, the carbonyl groups and hydroperoxide concentration were analyzed in plasma, and the fatty acid profiles of liver and heart microsomes were obtained. Results & discussion: only the hydroperoxides showed significant changes, while slight tendencies were observed in antioxidant activity and the carbonyl groups. Although the long term intake of White Tea and the administration of adriamycin did not change the fatty acid profile, slight tendencies existed for the SFAs, MUFAs and PUFAs.
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Molecular mechanisms by which White Tea prevents oxidative stress
Journal of Physiology and Biochemistry, 2014Co-Authors: Cristóbal Espinosa, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador Zamora, José Ángel López-jiménezAbstract:The flavonoid content of Tea ( Camellia sinensis ) has beneficial properties in the prevention of diseases. However, the mechanisms by which White Tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid Tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 ( Nrf2 ), NAD(P)H:quinone oxidoreductase 1 ( Nqo1 ), glutathione S-transferase ( Gst ), haem oxygenase-1 ( Ho1 ), catalase ( Cat ), superoxide dismutase ( Sod ) and glutathione reductase ( Gr ) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2 , Gst , Nqo1 , Ho1 , Cat , Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of White Tea increased in a higher degree the expression of Nrf2 , Gst , Nqo1 and Ho1 in the Tea + ADR group compared with the control group and C + ADR group. In addition, Tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner.
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Molecular mechanisms by which White Tea prevents oxidative stress
Journal of Physiology and Biochemistry, 2014Co-Authors: Cristóbal Espinosa, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador Zamora, José Ángel López-jiménezAbstract:The flavonoid content of Tea ( Camellia sinensis ) has beneficial properties in the prevention of diseases. However, the mechanisms by which White Tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid Tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 ( Nrf2 ), NAD(P)H:quinone oxidoreductase 1 ( Nqo1 ), glutathione S-transferase ( Gst ), haem oxygenase-1 ( Ho1 ), catalase ( Cat ), superoxide dismutase ( Sod ) and glutathione reductase ( Gr ) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2 , Gst , Nqo1 , Ho1 , Cat , Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of White Tea increased in a higher degree the expression of Nrf2 , Gst , Nqo1 and Ho1 in the Tea + ADR group compared with the control group and C + ADR group. In addition, Tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner.
Cristóbal Espinosa - One of the best experts on this subject based on the ideXlab platform.
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Long-term intake of White Tea prevents oxidative damage caused by adriamycin in kidney of rats.
Journal of the Science of Food and Agriculture, 2015Co-Authors: Cristóbal Espinosa, José Ángel López-jiménez, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador ZamoraAbstract:BACKGROUND White Tea infusion (Camelia sinensis) has antioxidants properties. The infusion contains polyphenols that have been proposed to induce antioxidant response element (ARE) response via nuclear factor E2-related factor 2 (NRF2). Adriamycin (ADR) has antitumour properties and oxidative effects. Oxidative stress is related to a variety of kidney diseases. Prevention of the oxidative stress through long-term intake of White Tea and the study of the molecular mechanisms involved in protection could be of great interest. Rats were given distilled water, 0.015 or 0.045 g of solid White Tea extract kg(-1) body weight for 12 months. Animals received an injection of ADR. In kidney, oxidative stress parameters were measured, the expressions of nuclear factor E2-related factor 2 gene (Nrf2), and detoxifying and antioxidants genes were analysed, and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured. RESULTS ADR administration increased oxidative parameters and decreased the antioxidant activity; significantly increased the expression of analysed genes and the activity of CAT and SOD and decreased GR activity. The highest White Tea dose protected redox status and inhibited ARE response. CONCLUSION Long-term intake of White Tea protected kidney against the oxidative stress. ADR activated the ARE response but in animals treated with the highest dose of White Tea, this response was inhibited, probably for antioxidant protection. © 2015 Society of Chemical Industry.
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Effect of long term intake of White Tea on acute oxidative stress in rats
Nutricion Hospitalaria, 2015Co-Authors: Cristóbal Espinosa, José Ángel López-jiménez, Francisca Pérez-llamas, Daniel González-silvera, Salvador ZamoraAbstract:Introduction: the well known antioxidant properties of White Tea include the prevention of cancer, neurodegenerative diseases and oxidative stress. Adriamycin can generate an amount of oxidative stress in vivo. Objective: evaluate long term intake of White Tea on plasma antioxidant capacity and on the fatty acid profile of liver and heart microsomes in animals subjected to acute oxidative stress. Methods: rats were given distilled water (controls), 15 mg/d (dose 1) or 45 mg/d (dose 2) of solid White Tea extract/per kilogram of body weight for 12 months. After this time, all the animals received an injection of adriamycin (ADR) (10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. Samples of plasma and liver and heart were taken. The antioxidant activity, the carbonyl groups and hydroperoxide concentration were analyzed in plasma, and the fatty acid profiles of liver and heart microsomes were obtained. Results & discussion: only the hydroperoxides showed significant changes, while slight tendencies were observed in antioxidant activity and the carbonyl groups. Although the long term intake of White Tea and the administration of adriamycin did not change the fatty acid profile, slight tendencies existed for the SFAs, MUFAs and PUFAs.
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Molecular mechanisms by which White Tea prevents oxidative stress
Journal of Physiology and Biochemistry, 2014Co-Authors: Cristóbal Espinosa, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador Zamora, José Ángel López-jiménezAbstract:The flavonoid content of Tea ( Camellia sinensis ) has beneficial properties in the prevention of diseases. However, the mechanisms by which White Tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid Tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 ( Nrf2 ), NAD(P)H:quinone oxidoreductase 1 ( Nqo1 ), glutathione S-transferase ( Gst ), haem oxygenase-1 ( Ho1 ), catalase ( Cat ), superoxide dismutase ( Sod ) and glutathione reductase ( Gr ) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2 , Gst , Nqo1 , Ho1 , Cat , Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of White Tea increased in a higher degree the expression of Nrf2 , Gst , Nqo1 and Ho1 in the Tea + ADR group compared with the control group and C + ADR group. In addition, Tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner.
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Molecular mechanisms by which White Tea prevents oxidative stress
Journal of Physiology and Biochemistry, 2014Co-Authors: Cristóbal Espinosa, Francisca Pérez-llamas, Francisco A. Guardiola, María Ángeles Esteban, Marino B. Arnao, Salvador Zamora, José Ángel López-jiménezAbstract:The flavonoid content of Tea ( Camellia sinensis ) has beneficial properties in the prevention of diseases. However, the mechanisms by which White Tea can protect against oxidative stress remain unclear. To shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid Tea extract/kg body weight for 12 months. All the animals received an injection of adriamycin (ADR; 10 mg/kg body weight), except half of the control group, which were given an injection of saline solution. The expression of the nuclear factor, E2-related factor 2 ( Nrf2 ), NAD(P)H:quinone oxidoreductase 1 ( Nqo1 ), glutathione S-transferase ( Gst ), haem oxygenase-1 ( Ho1 ), catalase ( Cat ), superoxide dismutase ( Sod ) and glutathione reductase ( Gr ) in liver was analysed by real-time PCR, and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) was measured spectrophotometrically. ADR significantly increased the expression of Nrf2 , Gst , Nqo1 , Ho1 , Cat , Sod and Gr with respect to the control levels and also increased the activity of CAT, SOD and GR. The intake of White Tea increased in a higher degree the expression of Nrf2 , Gst , Nqo1 and Ho1 in the Tea + ADR group compared with the control group and C + ADR group. In addition, Tea + ADR groups decreased the expression and activity of CAT, SOD and GR in a dose-dependent manner.
Asri Widyasanti - One of the best experts on this subject based on the ideXlab platform.
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The making of transparent soap based on olive oil with the addition of White Tea extract
Jurnal Penelitian Teh dan Kina, 2017Co-Authors: Asri Widyasanti, Jayanti Mega RohaniAbstract:Tea is a popular drink in the world. Types of Tea which is rarely used is White Tea. Besides consumed as a beverage, White Tea can also be used in extracted form. White Tea extract contains polyphenols compound, particularly catechins that can be used as an antibacterial. One of White Tea extract uses that can be added to production of olive oil transparent soap based. This study aimed to determine the process of transparent soap production, looking for the best concentration of White Tea extract, determine the characteristic and quality of transparent soap, and look for the extend of inhibition zone of Staphylococcus aureus in transparent soap. The treatments given in this research were the concentration of White Tea extract 1% (w / v) with the addition of A (control soap) = 0% (w / w), B = 0,5% (w / w), C = 1,0% (w / w), and D = 1,5% (w / w) of 300 grams of soap bases. Transparent soap characteristics observed were physical-chemical properties, antibacterial and organoleptic test. The analysis showed that all transparent soap baths complied the SNI 06-3532-1994 requirements of solid soap, except the water content and evaporated substance and the amount of fatty acids. The best result of this research was soap with treatment B (with 0,5% (w/w) extract addition), which was chosen based on organoleptic test (such as aroma, hardness and foam quantity) and Indonesian National Standard (SNI) qualities of solid soap with best result in unsaponified fraction test, hardness, and foam stability.
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The study of transparent soap making from virgin coconut oil-based with the addition of White Tea extract as an active ingredients
Jurnal Penelitian Teh dan Kina, 2017Co-Authors: Asri Widyasanti, Anditya Husnul HasnaAbstract:Transparent soap was made using high quality materials that beneficial for skin health and beauty. The main ingredient of the transparent soap was virgin coconut oil (VCO), with the addition of White Tea extract which was able to increase the benefits of transparent soap. This study aimed to create formulation of the transparent soap, to find the addition of the White Tea extracts to produce transparent soap and to study the characteristics of the transparent soap after the addition of White Tea extracts. The treatments in this study were the addition of White Tea extracts A = 0% (w / w), B = 0.5% (w / w, C = 1.0% (w / w and D = 1.5% (w / w). Observations on the transparent soap were including chemical properties, physical properties of soap, antibacterial and organoleptic test. The results of the analysis showed that all transparent soap formulas met the requirements of solid soap SNI 06-3532-1994, except for the total fatty acid. The formula of transparent soap with treatment B (addition of White Tea extract 0.5% w / v) was the best product with organoleptic test results reached 43.34%. The analysis results of those soap were 11.47% of water content and evaporating substances, 37.97% of total fatty acid, 0.519% of free fatty acid, 2.24% of unsaponified fraction, pH value of 9, hardness 0.0077 mm/g/sec, the foam stability of 76.88% and the activity of antibacterial inhibition diameter of 10.7 mm, respectively. This technology could be applied in the production of transparent soap with the addition of White Tea extract and to be developed into some industrial scale.
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Antibacteririal avtivities of Bacillus cereus and Shigella dysenteriae of White Tea extract in several variation of solvents
Jurnal Penelitian Teh dan Kina, 2016Co-Authors: Asri Widyasanti, Andita Mega Priantiwi, Dadan RohdianaAbstract:Tea is used in the food, cosmetic, and herbal drug industries. White Tea is a kind of Tea containing a relatively high polyphenol content compared to other kind of Tea, and thus has the potential of being used as a natural antibacterial. The objective of this research was to study the antibacterial activity of of White Tea extract in several variation of 13 % (w/v) concentration of solvents to the activity of gram-positive bacteria (Bacillus cereus) and Gram-negative bacteria (Shigella dysenteriae). Laboratorium experimental method was used in this descriptive study. The antibacterial activity study was comprised of estimating inhibition area and total of bacterial growth. The results revealed that White Tea extract inhibited bacterial growth, indicating that White Tea extract from the n-hexane, ethyl acetate and 96% ethanol solvents could be developed as an antibacterial material. The diameter of the inhibition area to Bacillus cereus bacteria for n - hexane , ethyl acetate, and ethanol 13 % (w/ v) solvents were 0.6 mm, 10.5 mm and 1.75 mm, respectively; while for Shigella dysenteriae bacteria were 0,5 mm, 9,86 mm and 10,6 mm, respectively. The total growth of Bacillus cereus bacteria for n-hexane, ethyl acetate, and ethanol 13 % (w/v) solvent were 1.3 x 10 7 , 6.0 x 10 6 , and 1.1 x 10 4 CFU / ml , and of Shigella dysenteriae bacteria were 1.0 x 10 7, 6.5 x 10 6 and 4.6 x 10 6 CFU / ml, respectively.
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Antibacterial activity of White Tea extract against gram positive and negative bacteria
2016Co-Authors: Asri Widyasanti, Siti Hajar, Dadan RohdianaAbstract:This research was carried out to determine antibacterial activity of White Tea extract against gram positive and gram negative bacteria based on its MIC ( m inimum i nhibitory c oncentration) . E thanol solution 96% were used to extract of White Tea with a ratio of 1:9 (w/v) by using maceration method . Paper disc diffusion method was used to determination of the MIC. T he concentration s were used of 2%, 4%, 6%, 8%, 10%, and 12% (w/v) . The results of this research showed that MIC of White Tea extract of 4%, 6%, 6% and 10% (w/v) against Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228, and Escherichia coli ATCC 25922, respectively. The inhibition zone for 4 bacteria were 10 . 83 mm, 10 . 08 mm, 10 . 16 mm and 12 . 08 mm respectively . This result of research concluded that White Tea extract have antibacterial activity against both gram positive and gram negative bacteria
