The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform
C. J. Dominic - One of the best experts on this subject based on the ideXlab platform.
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investigations on the olfactory discrimination exhibited by the females in the male induced implantation failure the bruce Effect in laboratory mice
Ethology, 2010Co-Authors: G. Rajendren, C. J. DominicAbstract:Direct contact with the alien male pheromone for 7 days before mating significantly decreased the rate of male-induced implantation failure (the Bruce Effect) in newly inseminated female mice. By contrast, females housed with alien males for 7 days before mating so that physical contact with the latter was prevented exhibited a high rate of implantation failure following exposure to alien males after mating. Exposure to an air-borne pheromone produced by alien males induced oestrus (the Whitten Effect) in a significant number of grouped females. The results do not support the suggestion that the inability of the stud male to block implantation is due to his original induction of oestrus. The results also do not support the view that the key factor in the induction of the Bruce Effect is the ability of the female to identify the stud as an individual which prevents her from responding to him but allows her to react to a new male with hormonal changes leading to implantation failure and return to oestrus. It is suggested that the olfactory discrimination exhibited by the female in the Bruce Effect is influenced by her overall olfactory experience before and after mating. The results are consistent with the view that the male-originating pheromone involved in the Bruce Effect is distinct from the one involved in the Whitten Effect.
Nadia Bellofiore - One of the best experts on this subject based on the ideXlab platform.
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pseudopregnancy and reproductive cycle synchronisation cannot be induced using conventional methods in the spiny mouse acomys cahirinus
Reproduction Fertility and Development, 2020Co-Authors: Nadia Bellofiore, Stacey J Ellery, Peter Templesmith, Jemma EvansAbstract:The menstruating spiny mouse is the first rodent identified to exhibit natural spontaneous decidualisation, cyclical endometrial shedding and regeneration. While the spiny mouse shares several primate-like characteristics in its reproductive biology, it has not been established whether pseudopregnancy can be induced or if its cycles can be synchronised as in non-human mammals. Here we describe attempts to induce pseudopregnancy and synchronisation of menstrual cycles (i.e. Whitten Effect) in spiny mice. Virgin females (n=3-8 per group) underwent one of the following procedures to induce pseudopregnancy: daily vaginal lavage only (control), progesterone injection, mechanical stimulation of the cervix and sterile mating. A separate cohort was also exposed to male-soiled bedding to assess the Whitten Effect. Pseudopregnancy was deemed successful if females presented with extended (>12 consecutive days) leukocytic vaginal cytology. No female from any method of induction met this criterion. In addition, the menstrual cycles of a group of six females could not be synchronised, nor immediate ovulation induced via exposure to male-soiled bedding. These responses indicate that the spiny mouse does not behave as a typical rodent. Like higher-order primates, the spiny mouse exhibits a relatively rare reproductive strategy, of failure to show pseudopregnancy or cyclical synchronisation. This is further endorsement of the use of this species as a versatile animal model for translational studies of menstruation and fertility.
Jemma Evans - One of the best experts on this subject based on the ideXlab platform.
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pseudopregnancy and reproductive cycle synchronisation cannot be induced using conventional methods in the spiny mouse acomys cahirinus
Reproduction Fertility and Development, 2020Co-Authors: Nadia Bellofiore, Stacey J Ellery, Peter Templesmith, Jemma EvansAbstract:The menstruating spiny mouse is the first rodent identified to exhibit natural spontaneous decidualisation, cyclical endometrial shedding and regeneration. While the spiny mouse shares several primate-like characteristics in its reproductive biology, it has not been established whether pseudopregnancy can be induced or if its cycles can be synchronised as in non-human mammals. Here we describe attempts to induce pseudopregnancy and synchronisation of menstrual cycles (i.e. Whitten Effect) in spiny mice. Virgin females (n=3-8 per group) underwent one of the following procedures to induce pseudopregnancy: daily vaginal lavage only (control), progesterone injection, mechanical stimulation of the cervix and sterile mating. A separate cohort was also exposed to male-soiled bedding to assess the Whitten Effect. Pseudopregnancy was deemed successful if females presented with extended (>12 consecutive days) leukocytic vaginal cytology. No female from any method of induction met this criterion. In addition, the menstrual cycles of a group of six females could not be synchronised, nor immediate ovulation induced via exposure to male-soiled bedding. These responses indicate that the spiny mouse does not behave as a typical rodent. Like higher-order primates, the spiny mouse exhibits a relatively rare reproductive strategy, of failure to show pseudopregnancy or cyclical synchronisation. This is further endorsement of the use of this species as a versatile animal model for translational studies of menstruation and fertility.
G. Rajendren - One of the best experts on this subject based on the ideXlab platform.
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investigations on the olfactory discrimination exhibited by the females in the male induced implantation failure the bruce Effect in laboratory mice
Ethology, 2010Co-Authors: G. Rajendren, C. J. DominicAbstract:Direct contact with the alien male pheromone for 7 days before mating significantly decreased the rate of male-induced implantation failure (the Bruce Effect) in newly inseminated female mice. By contrast, females housed with alien males for 7 days before mating so that physical contact with the latter was prevented exhibited a high rate of implantation failure following exposure to alien males after mating. Exposure to an air-borne pheromone produced by alien males induced oestrus (the Whitten Effect) in a significant number of grouped females. The results do not support the suggestion that the inability of the stud male to block implantation is due to his original induction of oestrus. The results also do not support the view that the key factor in the induction of the Bruce Effect is the ability of the female to identify the stud as an individual which prevents her from responding to him but allows her to react to a new male with hormonal changes leading to implantation failure and return to oestrus. It is suggested that the olfactory discrimination exhibited by the female in the Bruce Effect is influenced by her overall olfactory experience before and after mating. The results are consistent with the view that the male-originating pheromone involved in the Bruce Effect is distinct from the one involved in the Whitten Effect.
Sabrina Sukardi - One of the best experts on this subject based on the ideXlab platform.
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determination of the Whitten Effect based on vaginal cell characteristics vulva appearance and behavior in grouped female mice
Life Sciences Medicine and Biomedicine, 2019Co-Authors: Nur Hannan Zakaria, Sabrina SukardiAbstract:Introduction: Pheromones are chemicals produced by an animal that affects the behavior of another animal or the same species. Information conveyed includes location, presence of food or threat, sexual attraction, courtship, and dam–pup interactions. Pheromones are used widely in laboratory mice facilities to synchronize estrus and simultaneous breeding for logistic purposes. Female mice housed together in the absence of the male exhibit the Lee-Boot Effect of lengthened diestrus or ovarian inactive period of up to several weeks. Whitten Effect is described when a large number of female mice housed together in the absence of the male and having diestrus, will enter estrous 48 to 72 hours later upon exposure to male odors or male mouse urine soaked-bedding. Objective: The aim of this study is to determine the time taken for the Whitten Effect to occur based on changes in vaginal cell characteristics, vulva appearance and behavior in grouped female mice. Methodology: Ten female mice were acclimatized to the animal facility for 3 estrus cycles or 12 days. Phases of the estrus cycle were evaluated by visual observation to assess changes to the vulva and vaginal cytology. Male urine soaked-bedding were exposed to females for 3 days and the time taken for the Whitten Effect to occur was determined based on changes in vaginal cell characteristics, vulva appearance and observation of behavior. Result: The Cochran's Q test was used to observe the changes from diestrus to proestrus and later estrus. The results showed a significant difference (p<0.05) in the number of mice that successfully enter the proestrus and estrus phases over a four time point, ?2 (2) = 18.857. Conclusion: The Whitten Effect occurs after 72 hours of exposure to male urine soaked-bedding based on vulva appearance, vaginal cell characteristics and behavior in grouped female mice.
