The Experts below are selected from a list of 4143 Experts worldwide ranked by ideXlab platform
Wafaa M.k. Bakr - One of the best experts on this subject based on the ideXlab platform.
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TUBEX Test Versus Widal Test In The Diagnosis Of Typhoid Fever In Kafr El-Shekh,
2015Co-Authors: Wafaa M.k. Bakr, Laila El A. Attar, Medhat S. Ashour, Ayman El M. TokhyAbstract:Background: The value of the Widal Test for the diagnosis of typhoid fever has been debated for as many years as it has been available. TUBEX Test is a serological Test which was stated to have the advantages of the Widal Test without its controversies. The aim of this study was to evaluate TUBEX Test versus the Widal Test regarding sensitivity and specificity for the diagnosis of typhoid fever in an endemic area like Kafr El –Shekh, Egypt. Materials and Methods: Serum samples were collected from typhoid (n=91) and febrile non-typhoid patients (n=25) and used to evaluate the performance of both Widal and TUBEX Tests in diagnosis of typhoid fever using IgM anti-LPS ELISA as a reference Test. Results: TUBEX Test had sensitivity, specificity, accuracy, positive predictive value and negative predictive value of 74.6%, 75%, 74.7%, 89.2 % and 58 % respectively. Widal Test had higher results. Conclusion and recommendation: TUBEX Test results are not superior to Widal Test. TUBEX has a very serious shortcoming regarding its color scoring system. We do not recommend the use of TUBEX Test for diagnosis of typhoid fever in Egypt as Widal Test which is the Test commonly used in diagnosis gave better performance
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The dilemma of Widal Test - which brand to use? a study of four different Widal brands: a cross sectional comparative study.
Annals of Clinical Microbiology and Antimicrobials, 2011Co-Authors: Wafaa M.k. Bakr, Laila El Attar, Mohammed Seif El-din Ashour, Ayman M El ToukhyAbstract:Background Serodiagnosis of typhoid fever by Widal Test based on demonstrating the presence of agglutinins (antibodies) in the serum of an infected patient, against the H (flagellar) and O (somatic) antigens of Salmonella enterica serotype Typhi has been associated with many debates. This is why the aim of this study was to: (i) Compare the diagnostic accuracy of four different commercial kits used to perform Widal Test (Remel, BioSystems, Dialab and Biotec). (ii) Compare the sensitivity and specificity of both anti-O and anti-H antibodies. (iii) Compare the validity of single versus paired serum samples with a rising titer for the diagnosis of typhoid fever.
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TUBEX Test Versus Widal Test In The Diagnosis Of Typhoid Fever In Kafr El -Shekh, Egypt.
The Journal of the Egyptian Public Health Association, 2010Co-Authors: Wafaa M.k. Bakr, Laila El Attar, Medhat S. Ashour, Ayman El M. TokhyAbstract:Background: The value of the Widal Test for the diagnosis of typhoid fever has been debated for as many years as it has been available. TUBEX Test is a serological Test which was stated to have the advantages of the Widal Test without its controversies. The aim of this study was to evaluate TUBEX Test versus the Widal Test regarding sensitivity and specificity for the diagnosis of typhoid fever in an endemic area like Kafr El –Shekh, Egypt. Materials and Methods: Serum samples were collected from typhoid (n=91) and febrile non-typhoid patients (n=25) and used to evaluate the performance of both Widal and TUBEX Tests in diagnosis of typhoid fever using IgM anti-LPS ELISA as a reference Test. Results: TUBEX Test had sensitivity, specificity, accuracy, positive predictive value and negative predictive value of 74.6%, 75%, 74.7%, 89.2% and 58% respectively. Widal Test had higher results. Conclusion and recommendation: TUBEX Test results are not superior to Widal Test. TUBEX has a very serious shortcoming regarding its color scoring system. We do not recommend the use of TUBEX Test for diagnosis of typhoid fever in Egypt as Widal Test which is the Test commonly used in diagnosis gave better performance.
Mecky I. Matee - One of the best experts on this subject based on the ideXlab platform.
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Performance of Widal Test and stool culture in the diagnosis of typhoid fever among suspected patients in Dar es Salaam, Tanzania.
BMC research notes, 2019Co-Authors: Akili Mawazo, George M. Bwire, Mecky I. MateeAbstract:We set an experiment to determine the diagnostic performance of the Widal Test and stool culture in typhoid-suspected cases attending tertiary hospitals in Dar es Salaam, Tanzania using blood culture as a golden standard. We also evaluated the agreement between Widal, stool and blood culture. This was a cross-sectional study conducted between June and September 2018, in three Regional Referral Hospitals in Dar es Salaam, Tanzania. A total of 158 typhoid-suspected cases were enrolled, after obtaining an informed consent. Of the 158 patients participated in the study, 128 (81%) Tested positive for the Widal Test and 17 (11%) patients were stool culture positive. Widal Test recorded 81.5% sensitivity, 18.3% specificity, 10.1% positive predictive value and 89.7% negative predictive value. Stool culture showed 31.3% sensitivity, 91.5% specificity, 29% positive predictive value and 91.5% negative predictive value. In conclusion, Widal Test is not reliable for diagnosis of typhoid fever since false positive and negative results are common. In addition, Widal Test recorded poor agreement with the blood culture (kappa = 0.014, p
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performance of Widal Test and stool culture in the diagnosis of typhoid fever among suspected patients in dar es salaam tanzania
BMC Research Notes, 2019Co-Authors: Akili Mawazo, George M. Bwire, Mecky I. MateeAbstract:We set an experiment to determine the diagnostic performance of the Widal Test and stool culture in typhoid-suspected cases attending tertiary hospitals in Dar es Salaam, Tanzania using blood culture as a golden standard. We also evaluated the agreement between Widal, stool and blood culture. This was a cross-sectional study conducted between June and September 2018, in three Regional Referral Hospitals in Dar es Salaam, Tanzania. A total of 158 typhoid-suspected cases were enrolled, after obtaining an informed consent. Of the 158 patients participated in the study, 128 (81%) Tested positive for the Widal Test and 17 (11%) patients were stool culture positive. Widal Test recorded 81.5% sensitivity, 18.3% specificity, 10.1% positive predictive value and 89.7% negative predictive value. Stool culture showed 31.3% sensitivity, 91.5% specificity, 29% positive predictive value and 91.5% negative predictive value. In conclusion, Widal Test is not reliable for diagnosis of typhoid fever since false positive and negative results are common. In addition, Widal Test recorded poor agreement with the blood culture (kappa = 0.014, p < 0.05) while stool culture had strong agreement with the blood culture (kappa = 0.22, p < 0.05).
Laila El Attar - One of the best experts on this subject based on the ideXlab platform.
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The dilemma of Widal Test - which brand to use? a study of four different Widal brands: a cross sectional comparative study.
Annals of Clinical Microbiology and Antimicrobials, 2011Co-Authors: Wafaa M.k. Bakr, Laila El Attar, Mohammed Seif El-din Ashour, Ayman M El ToukhyAbstract:Background Serodiagnosis of typhoid fever by Widal Test based on demonstrating the presence of agglutinins (antibodies) in the serum of an infected patient, against the H (flagellar) and O (somatic) antigens of Salmonella enterica serotype Typhi has been associated with many debates. This is why the aim of this study was to: (i) Compare the diagnostic accuracy of four different commercial kits used to perform Widal Test (Remel, BioSystems, Dialab and Biotec). (ii) Compare the sensitivity and specificity of both anti-O and anti-H antibodies. (iii) Compare the validity of single versus paired serum samples with a rising titer for the diagnosis of typhoid fever.
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TUBEX Test Versus Widal Test In The Diagnosis Of Typhoid Fever In Kafr El -Shekh, Egypt.
The Journal of the Egyptian Public Health Association, 2010Co-Authors: Wafaa M.k. Bakr, Laila El Attar, Medhat S. Ashour, Ayman El M. TokhyAbstract:Background: The value of the Widal Test for the diagnosis of typhoid fever has been debated for as many years as it has been available. TUBEX Test is a serological Test which was stated to have the advantages of the Widal Test without its controversies. The aim of this study was to evaluate TUBEX Test versus the Widal Test regarding sensitivity and specificity for the diagnosis of typhoid fever in an endemic area like Kafr El –Shekh, Egypt. Materials and Methods: Serum samples were collected from typhoid (n=91) and febrile non-typhoid patients (n=25) and used to evaluate the performance of both Widal and TUBEX Tests in diagnosis of typhoid fever using IgM anti-LPS ELISA as a reference Test. Results: TUBEX Test had sensitivity, specificity, accuracy, positive predictive value and negative predictive value of 74.6%, 75%, 74.7%, 89.2% and 58% respectively. Widal Test had higher results. Conclusion and recommendation: TUBEX Test results are not superior to Widal Test. TUBEX has a very serious shortcoming regarding its color scoring system. We do not recommend the use of TUBEX Test for diagnosis of typhoid fever in Egypt as Widal Test which is the Test commonly used in diagnosis gave better performance.
Ayman El M. Tokhy - One of the best experts on this subject based on the ideXlab platform.
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TUBEX Test Versus Widal Test In The Diagnosis Of Typhoid Fever In Kafr El-Shekh,
2015Co-Authors: Wafaa M.k. Bakr, Laila El A. Attar, Medhat S. Ashour, Ayman El M. TokhyAbstract:Background: The value of the Widal Test for the diagnosis of typhoid fever has been debated for as many years as it has been available. TUBEX Test is a serological Test which was stated to have the advantages of the Widal Test without its controversies. The aim of this study was to evaluate TUBEX Test versus the Widal Test regarding sensitivity and specificity for the diagnosis of typhoid fever in an endemic area like Kafr El –Shekh, Egypt. Materials and Methods: Serum samples were collected from typhoid (n=91) and febrile non-typhoid patients (n=25) and used to evaluate the performance of both Widal and TUBEX Tests in diagnosis of typhoid fever using IgM anti-LPS ELISA as a reference Test. Results: TUBEX Test had sensitivity, specificity, accuracy, positive predictive value and negative predictive value of 74.6%, 75%, 74.7%, 89.2 % and 58 % respectively. Widal Test had higher results. Conclusion and recommendation: TUBEX Test results are not superior to Widal Test. TUBEX has a very serious shortcoming regarding its color scoring system. We do not recommend the use of TUBEX Test for diagnosis of typhoid fever in Egypt as Widal Test which is the Test commonly used in diagnosis gave better performance
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TUBEX Test Versus Widal Test In The Diagnosis Of Typhoid Fever In Kafr El -Shekh, Egypt.
The Journal of the Egyptian Public Health Association, 2010Co-Authors: Wafaa M.k. Bakr, Laila El Attar, Medhat S. Ashour, Ayman El M. TokhyAbstract:Background: The value of the Widal Test for the diagnosis of typhoid fever has been debated for as many years as it has been available. TUBEX Test is a serological Test which was stated to have the advantages of the Widal Test without its controversies. The aim of this study was to evaluate TUBEX Test versus the Widal Test regarding sensitivity and specificity for the diagnosis of typhoid fever in an endemic area like Kafr El –Shekh, Egypt. Materials and Methods: Serum samples were collected from typhoid (n=91) and febrile non-typhoid patients (n=25) and used to evaluate the performance of both Widal and TUBEX Tests in diagnosis of typhoid fever using IgM anti-LPS ELISA as a reference Test. Results: TUBEX Test had sensitivity, specificity, accuracy, positive predictive value and negative predictive value of 74.6%, 75%, 74.7%, 89.2% and 58% respectively. Widal Test had higher results. Conclusion and recommendation: TUBEX Test results are not superior to Widal Test. TUBEX has a very serious shortcoming regarding its color scoring system. We do not recommend the use of TUBEX Test for diagnosis of typhoid fever in Egypt as Widal Test which is the Test commonly used in diagnosis gave better performance.
Akili Mawazo - One of the best experts on this subject based on the ideXlab platform.
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Performance of Widal Test and stool culture in the diagnosis of typhoid fever among suspected patients in Dar es Salaam, Tanzania.
BMC research notes, 2019Co-Authors: Akili Mawazo, George M. Bwire, Mecky I. MateeAbstract:We set an experiment to determine the diagnostic performance of the Widal Test and stool culture in typhoid-suspected cases attending tertiary hospitals in Dar es Salaam, Tanzania using blood culture as a golden standard. We also evaluated the agreement between Widal, stool and blood culture. This was a cross-sectional study conducted between June and September 2018, in three Regional Referral Hospitals in Dar es Salaam, Tanzania. A total of 158 typhoid-suspected cases were enrolled, after obtaining an informed consent. Of the 158 patients participated in the study, 128 (81%) Tested positive for the Widal Test and 17 (11%) patients were stool culture positive. Widal Test recorded 81.5% sensitivity, 18.3% specificity, 10.1% positive predictive value and 89.7% negative predictive value. Stool culture showed 31.3% sensitivity, 91.5% specificity, 29% positive predictive value and 91.5% negative predictive value. In conclusion, Widal Test is not reliable for diagnosis of typhoid fever since false positive and negative results are common. In addition, Widal Test recorded poor agreement with the blood culture (kappa = 0.014, p
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performance of Widal Test and stool culture in the diagnosis of typhoid fever among suspected patients in dar es salaam tanzania
BMC Research Notes, 2019Co-Authors: Akili Mawazo, George M. Bwire, Mecky I. MateeAbstract:We set an experiment to determine the diagnostic performance of the Widal Test and stool culture in typhoid-suspected cases attending tertiary hospitals in Dar es Salaam, Tanzania using blood culture as a golden standard. We also evaluated the agreement between Widal, stool and blood culture. This was a cross-sectional study conducted between June and September 2018, in three Regional Referral Hospitals in Dar es Salaam, Tanzania. A total of 158 typhoid-suspected cases were enrolled, after obtaining an informed consent. Of the 158 patients participated in the study, 128 (81%) Tested positive for the Widal Test and 17 (11%) patients were stool culture positive. Widal Test recorded 81.5% sensitivity, 18.3% specificity, 10.1% positive predictive value and 89.7% negative predictive value. Stool culture showed 31.3% sensitivity, 91.5% specificity, 29% positive predictive value and 91.5% negative predictive value. In conclusion, Widal Test is not reliable for diagnosis of typhoid fever since false positive and negative results are common. In addition, Widal Test recorded poor agreement with the blood culture (kappa = 0.014, p < 0.05) while stool culture had strong agreement with the blood culture (kappa = 0.22, p < 0.05).
