The Experts below are selected from a list of 89265 Experts worldwide ranked by ideXlab platform
Zeynal Topalcengiz - One of the best experts on this subject based on the ideXlab platform.
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survival of shiga toxin producing escherichia coli in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Fei Wang, Robert L Buchanan, Jeffrey T LejeuneAbstract:ABSTRACT Domestic and Wild Animal intrusions are identified as a food safety risk during fresh produce production. The purpose of this study was to evaluate the survival of Shiga toxin-producing Escherichia coli (STEC) in cattle, feral pig, waterfowl, deer, and raccoon feces from sources in California, Delaware, Florida, and Ohio. Fecal samples were inoculated with a cocktail of rifampin-resistant STEC serotypes (O103, O104, O111, O145, and O157) (104 to 106 CFU/g of feces). Inoculated feces were held at ambient temperature. Populations of surviving cells were monitored throughout 1 year (364 days), with viable populations being enumerated by spread plating and enrichment when the bacteria were no longer detected by plating. Representative colonies were collected at various time intervals based on availability from different locations to determine the persistence of surviving STEC serotypes. Over the 364-day storage period, similar survival trends were observed for each type of Animal feces from all states except for cattle and deer feces from Ohio. STEC populations remained the highest in cattle and deer feces from all states between days 28 and 364, except for those from Ohio. Feral pig, waterfowl, and raccoon feces had populations of STEC of <1.0 log CFU/g starting from day 112 in feces from all states. E. coli O103 and O104 were the predominant serotypes throughout the entire storage period in feces from all Animals and from all states. The survival of both O157 and non-O157 STEC strains in domesticated and Wild Animal feces indicates a potential risk of contamination from Animal intrusion. HIGHLIGHTS
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survival of salmonella in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Robert L Buchanan, Joyjit Saha, Jeffrey T LejeuneAbstract:ABSTRACT Heightened concerns about Wildlife on produce farms and possible introduction of pathogens to the food supply have resulted in required actions following intrusion events. The purpose of this study was to evaluate the survival of Salmonella in feces from cattle and various Wild Animals (feral pigs, waterfowl, deer, and raccoons) in California, Delaware, Florida, and Ohio. Feces were inoculated with rifampin-resistant Salmonella enterica cocktails that included six serotypes: Typhimurium, Montevideo, Anatum, Javiana, Braenderup, and Newport (104 to 106 CFU/g). Fecal samples were stored at ambient temperature. Populations were enumerated for up to 1 year (364 days) by spread plating onto tryptic soy agar supplemented with rifampin. When no colonies were detected, samples were enriched. Colonies were banked on various sampling days based on availability of serotyping in each state. During the 364-day storage period, Salmonella populations decreased to ≤2.0 log CFU/g by day 84 in pig, waterfowl, and raccoon feces from all states. Salmonella populations in cattle and deer feces were 3.3 to 6.1 log CFU/g on day 336 or 364; however, in Ohio Salmonella was not detected after 120 days. Salmonella serotypes Anatum, Braenderup, and Javiana were the predominant serotypes throughout the storage period in all Animal feces and states. Determination of appropriate risk mitigation strategies following Animal intrusions can improve our understanding of pathogen survival in Animal feces. HIGHLIGHTS
Jeffrey T Lejeune - One of the best experts on this subject based on the ideXlab platform.
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survival of shiga toxin producing escherichia coli in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Fei Wang, Robert L Buchanan, Jeffrey T LejeuneAbstract:ABSTRACT Domestic and Wild Animal intrusions are identified as a food safety risk during fresh produce production. The purpose of this study was to evaluate the survival of Shiga toxin-producing Escherichia coli (STEC) in cattle, feral pig, waterfowl, deer, and raccoon feces from sources in California, Delaware, Florida, and Ohio. Fecal samples were inoculated with a cocktail of rifampin-resistant STEC serotypes (O103, O104, O111, O145, and O157) (104 to 106 CFU/g of feces). Inoculated feces were held at ambient temperature. Populations of surviving cells were monitored throughout 1 year (364 days), with viable populations being enumerated by spread plating and enrichment when the bacteria were no longer detected by plating. Representative colonies were collected at various time intervals based on availability from different locations to determine the persistence of surviving STEC serotypes. Over the 364-day storage period, similar survival trends were observed for each type of Animal feces from all states except for cattle and deer feces from Ohio. STEC populations remained the highest in cattle and deer feces from all states between days 28 and 364, except for those from Ohio. Feral pig, waterfowl, and raccoon feces had populations of STEC of <1.0 log CFU/g starting from day 112 in feces from all states. E. coli O103 and O104 were the predominant serotypes throughout the entire storage period in feces from all Animals and from all states. The survival of both O157 and non-O157 STEC strains in domesticated and Wild Animal feces indicates a potential risk of contamination from Animal intrusion. HIGHLIGHTS
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survival of salmonella in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Robert L Buchanan, Joyjit Saha, Jeffrey T LejeuneAbstract:ABSTRACT Heightened concerns about Wildlife on produce farms and possible introduction of pathogens to the food supply have resulted in required actions following intrusion events. The purpose of this study was to evaluate the survival of Salmonella in feces from cattle and various Wild Animals (feral pigs, waterfowl, deer, and raccoons) in California, Delaware, Florida, and Ohio. Feces were inoculated with rifampin-resistant Salmonella enterica cocktails that included six serotypes: Typhimurium, Montevideo, Anatum, Javiana, Braenderup, and Newport (104 to 106 CFU/g). Fecal samples were stored at ambient temperature. Populations were enumerated for up to 1 year (364 days) by spread plating onto tryptic soy agar supplemented with rifampin. When no colonies were detected, samples were enriched. Colonies were banked on various sampling days based on availability of serotyping in each state. During the 364-day storage period, Salmonella populations decreased to ≤2.0 log CFU/g by day 84 in pig, waterfowl, and raccoon feces from all states. Salmonella populations in cattle and deer feces were 3.3 to 6.1 log CFU/g on day 336 or 364; however, in Ohio Salmonella was not detected after 120 days. Salmonella serotypes Anatum, Braenderup, and Javiana were the predominant serotypes throughout the storage period in all Animal feces and states. Determination of appropriate risk mitigation strategies following Animal intrusions can improve our understanding of pathogen survival in Animal feces. HIGHLIGHTS
Saharuetai Jeamsripong - One of the best experts on this subject based on the ideXlab platform.
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survival of shiga toxin producing escherichia coli in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Fei Wang, Robert L Buchanan, Jeffrey T LejeuneAbstract:ABSTRACT Domestic and Wild Animal intrusions are identified as a food safety risk during fresh produce production. The purpose of this study was to evaluate the survival of Shiga toxin-producing Escherichia coli (STEC) in cattle, feral pig, waterfowl, deer, and raccoon feces from sources in California, Delaware, Florida, and Ohio. Fecal samples were inoculated with a cocktail of rifampin-resistant STEC serotypes (O103, O104, O111, O145, and O157) (104 to 106 CFU/g of feces). Inoculated feces were held at ambient temperature. Populations of surviving cells were monitored throughout 1 year (364 days), with viable populations being enumerated by spread plating and enrichment when the bacteria were no longer detected by plating. Representative colonies were collected at various time intervals based on availability from different locations to determine the persistence of surviving STEC serotypes. Over the 364-day storage period, similar survival trends were observed for each type of Animal feces from all states except for cattle and deer feces from Ohio. STEC populations remained the highest in cattle and deer feces from all states between days 28 and 364, except for those from Ohio. Feral pig, waterfowl, and raccoon feces had populations of STEC of <1.0 log CFU/g starting from day 112 in feces from all states. E. coli O103 and O104 were the predominant serotypes throughout the entire storage period in feces from all Animals and from all states. The survival of both O157 and non-O157 STEC strains in domesticated and Wild Animal feces indicates a potential risk of contamination from Animal intrusion. HIGHLIGHTS
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survival of salmonella in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Robert L Buchanan, Joyjit Saha, Jeffrey T LejeuneAbstract:ABSTRACT Heightened concerns about Wildlife on produce farms and possible introduction of pathogens to the food supply have resulted in required actions following intrusion events. The purpose of this study was to evaluate the survival of Salmonella in feces from cattle and various Wild Animals (feral pigs, waterfowl, deer, and raccoons) in California, Delaware, Florida, and Ohio. Feces were inoculated with rifampin-resistant Salmonella enterica cocktails that included six serotypes: Typhimurium, Montevideo, Anatum, Javiana, Braenderup, and Newport (104 to 106 CFU/g). Fecal samples were stored at ambient temperature. Populations were enumerated for up to 1 year (364 days) by spread plating onto tryptic soy agar supplemented with rifampin. When no colonies were detected, samples were enriched. Colonies were banked on various sampling days based on availability of serotyping in each state. During the 364-day storage period, Salmonella populations decreased to ≤2.0 log CFU/g by day 84 in pig, waterfowl, and raccoon feces from all states. Salmonella populations in cattle and deer feces were 3.3 to 6.1 log CFU/g on day 336 or 364; however, in Ohio Salmonella was not detected after 120 days. Salmonella serotypes Anatum, Braenderup, and Javiana were the predominant serotypes throughout the storage period in all Animal feces and states. Determination of appropriate risk mitigation strategies following Animal intrusions can improve our understanding of pathogen survival in Animal feces. HIGHLIGHTS
Anil K Persad - One of the best experts on this subject based on the ideXlab platform.
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survival of shiga toxin producing escherichia coli in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Fei Wang, Robert L Buchanan, Jeffrey T LejeuneAbstract:ABSTRACT Domestic and Wild Animal intrusions are identified as a food safety risk during fresh produce production. The purpose of this study was to evaluate the survival of Shiga toxin-producing Escherichia coli (STEC) in cattle, feral pig, waterfowl, deer, and raccoon feces from sources in California, Delaware, Florida, and Ohio. Fecal samples were inoculated with a cocktail of rifampin-resistant STEC serotypes (O103, O104, O111, O145, and O157) (104 to 106 CFU/g of feces). Inoculated feces were held at ambient temperature. Populations of surviving cells were monitored throughout 1 year (364 days), with viable populations being enumerated by spread plating and enrichment when the bacteria were no longer detected by plating. Representative colonies were collected at various time intervals based on availability from different locations to determine the persistence of surviving STEC serotypes. Over the 364-day storage period, similar survival trends were observed for each type of Animal feces from all states except for cattle and deer feces from Ohio. STEC populations remained the highest in cattle and deer feces from all states between days 28 and 364, except for those from Ohio. Feral pig, waterfowl, and raccoon feces had populations of STEC of <1.0 log CFU/g starting from day 112 in feces from all states. E. coli O103 and O104 were the predominant serotypes throughout the entire storage period in feces from all Animals and from all states. The survival of both O157 and non-O157 STEC strains in domesticated and Wild Animal feces indicates a potential risk of contamination from Animal intrusion. HIGHLIGHTS
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survival of salmonella in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Robert L Buchanan, Joyjit Saha, Jeffrey T LejeuneAbstract:ABSTRACT Heightened concerns about Wildlife on produce farms and possible introduction of pathogens to the food supply have resulted in required actions following intrusion events. The purpose of this study was to evaluate the survival of Salmonella in feces from cattle and various Wild Animals (feral pigs, waterfowl, deer, and raccoons) in California, Delaware, Florida, and Ohio. Feces were inoculated with rifampin-resistant Salmonella enterica cocktails that included six serotypes: Typhimurium, Montevideo, Anatum, Javiana, Braenderup, and Newport (104 to 106 CFU/g). Fecal samples were stored at ambient temperature. Populations were enumerated for up to 1 year (364 days) by spread plating onto tryptic soy agar supplemented with rifampin. When no colonies were detected, samples were enriched. Colonies were banked on various sampling days based on availability of serotyping in each state. During the 364-day storage period, Salmonella populations decreased to ≤2.0 log CFU/g by day 84 in pig, waterfowl, and raccoon feces from all states. Salmonella populations in cattle and deer feces were 3.3 to 6.1 log CFU/g on day 336 or 364; however, in Ohio Salmonella was not detected after 120 days. Salmonella serotypes Anatum, Braenderup, and Javiana were the predominant serotypes throughout the storage period in all Animal feces and states. Determination of appropriate risk mitigation strategies following Animal intrusions can improve our understanding of pathogen survival in Animal feces. HIGHLIGHTS
Robert L Buchanan - One of the best experts on this subject based on the ideXlab platform.
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survival of shiga toxin producing escherichia coli in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Fei Wang, Robert L Buchanan, Jeffrey T LejeuneAbstract:ABSTRACT Domestic and Wild Animal intrusions are identified as a food safety risk during fresh produce production. The purpose of this study was to evaluate the survival of Shiga toxin-producing Escherichia coli (STEC) in cattle, feral pig, waterfowl, deer, and raccoon feces from sources in California, Delaware, Florida, and Ohio. Fecal samples were inoculated with a cocktail of rifampin-resistant STEC serotypes (O103, O104, O111, O145, and O157) (104 to 106 CFU/g of feces). Inoculated feces were held at ambient temperature. Populations of surviving cells were monitored throughout 1 year (364 days), with viable populations being enumerated by spread plating and enrichment when the bacteria were no longer detected by plating. Representative colonies were collected at various time intervals based on availability from different locations to determine the persistence of surviving STEC serotypes. Over the 364-day storage period, similar survival trends were observed for each type of Animal feces from all states except for cattle and deer feces from Ohio. STEC populations remained the highest in cattle and deer feces from all states between days 28 and 364, except for those from Ohio. Feral pig, waterfowl, and raccoon feces had populations of STEC of <1.0 log CFU/g starting from day 112 in feces from all states. E. coli O103 and O104 were the predominant serotypes throughout the entire storage period in feces from all Animals and from all states. The survival of both O157 and non-O157 STEC strains in domesticated and Wild Animal feces indicates a potential risk of contamination from Animal intrusion. HIGHLIGHTS
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survival of salmonella in various Wild Animal feces that may contaminate produce
Journal of Food Protection, 2020Co-Authors: Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M Spanninger, Anil K Persad, Robert L Buchanan, Joyjit Saha, Jeffrey T LejeuneAbstract:ABSTRACT Heightened concerns about Wildlife on produce farms and possible introduction of pathogens to the food supply have resulted in required actions following intrusion events. The purpose of this study was to evaluate the survival of Salmonella in feces from cattle and various Wild Animals (feral pigs, waterfowl, deer, and raccoons) in California, Delaware, Florida, and Ohio. Feces were inoculated with rifampin-resistant Salmonella enterica cocktails that included six serotypes: Typhimurium, Montevideo, Anatum, Javiana, Braenderup, and Newport (104 to 106 CFU/g). Fecal samples were stored at ambient temperature. Populations were enumerated for up to 1 year (364 days) by spread plating onto tryptic soy agar supplemented with rifampin. When no colonies were detected, samples were enriched. Colonies were banked on various sampling days based on availability of serotyping in each state. During the 364-day storage period, Salmonella populations decreased to ≤2.0 log CFU/g by day 84 in pig, waterfowl, and raccoon feces from all states. Salmonella populations in cattle and deer feces were 3.3 to 6.1 log CFU/g on day 336 or 364; however, in Ohio Salmonella was not detected after 120 days. Salmonella serotypes Anatum, Braenderup, and Javiana were the predominant serotypes throughout the storage period in all Animal feces and states. Determination of appropriate risk mitigation strategies following Animal intrusions can improve our understanding of pathogen survival in Animal feces. HIGHLIGHTS
