The Experts below are selected from a list of 18090 Experts worldwide ranked by ideXlab platform
Qirong Shen - One of the best experts on this subject based on the ideXlab platform.
-
fumigation coupled with bio organic fertilizer for the suppression of watermelon fusarium Wilt Disease re shapes the soil microbiome
Applied Soil Ecology, 2019Co-Authors: Chao Xue, Zongzhuan Shen, Yuewen Hao, Weijie Huang, Yao Chong, Wei Ran, Qirong ShenAbstract:Abstract Fusarium Wilt of watermelon caused by Fusarium oxysporums f. sp. niveum (FON) is the most destructive Disease impacting production. Soil fumigation using ammonium bicarbonate coupled with bioorganic fertilizer (BOF) application was conducted in the field in order to suppress Fusarium Wilt Disease. The composition of the soil microbiome after fumigation and at harvest was assessed using MiSeq high throughput sequencing. Soil fumigation succeeded in suppressing Disease incidence, with a drop from 96% to
-
banana fusarium Wilt Disease incidence is influenced by shifts of soil microbial communities under different monoculture spans
Microbial Ecology, 2018Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Ryan C Penton, Xianfu Yuan, Qirong ShenAbstract:The continuous cropping of banana in the same field may result in a serious soil-borne Fusarium Wilt Disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium Wilt Disease in the present study. The results showed that successive cropping of banana was significantly correlated with the Fusarium Wilt Disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium Wilt Disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and Disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower Disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium Wilt Disease.
-
Microflora that harbor the NRPS gene are responsible for Fusarium Wilt Disease-suppressive soil
Applied Soil Ecology, 2018Co-Authors: Mengli Zhao, Ruifu Zhang, Xuhui Deng, Jun Yuan, Menghui Dong, Chengzhi Zhu, Qirong ShenAbstract:Abstract Non-ribosomal peptides (NRPs) are one of the largest groups of natural microbial secondary metabolites, which include peptides such as the antibiotics vancomycin and gramicidin, as well as lipopeptides (surfactin, iturin A and bacillomycin). In this study, banana Fusarium Wilt Disease suppressive and conducive soils were chosen to investigate the role of microbes that harbor the NRPS gene in Disease suppression based on the 454-pyrosequencing platform and real-time PCR technique. The results showed that higher abundances and diversity of microbes that harbor the NRPS gene were observed in the suppressive soil samples than in the conducive soil. According to the results of the DNA sequences blastx of NRPS, the main microbial taxa harboring the NRPS gene were identified, and Pseudomonas in Proteobacteria and Streptomyces in Actinobacteria might be remarkably related to Fusarium Wilt Disease suppression. Furthermore, the Mantel test showed that compared with bacteria community and chemical properties, the microbial community harboring the NRPS gene had a more significant impact on the Disease incidences of Fusarium Wilt. This study provided non-specific relationships between groups of microbes harboring NRPS genes and Fusarium Wilt Disease suppression suggesting potential interaction based on correlation evidence, and pointed out a potential mechanism of suppressive soil.
-
Distinct roles for soil fungal and bacterial communities associated with the suppression of vanilla Fusarium Wilt Disease
Soil Biology and Biochemistry, 2017Co-Authors: Wu Xiong, Qingyun Zhao, Yi Ren, Chen Liu, Alexandre Jousset, Qirong ShenAbstract:Characterizing microbial communities associated with Disease-suppressive soil is an important first step toward understanding the potential of microbiota to protect crops against plant pathogens. In the present study, we compared microbial communities in suppressive- and conducive-soils associated with Fusarium Wilt Disease in a vanilla long-term continuous cropping system. Suppressive soil was associated with higher fungal diversity and lower bacterial diversity. The fungal phyla Zygomycota and Basidiomycota, and the bacterial phyla Acidobacteria, Verrucomicrobia, Actinobacteria and Firmicutes were strongly enriched in the suppressive soil. Notably, suppressive soil was dominated by the fungal genus Mortierella, accounting for 37% of the total fungal sequences. The hyper-dominance of Mortierella spp. in suppressive soil suggests that this taxon may serve as an indicator and enhancer of Fusarium Wilt Disease suppression in vanilla. In addition, Molecular Ecological Network analysis revealed that fungal communities were more connected and showed more co-occurrence relationships in the suppressive versus conducive soils. Our results indicate that fungal communities may be important in the development of soil suppressiveness against vanilla Fusarium Wilt Disease.
-
comparison of fungal community in black pepper vanilla and vanilla monoculture systems associated with vanilla fusarium Wilt Disease
Frontiers in Microbiology, 2016Co-Authors: Wu Xiong, Huasong Wu, Qingyun Zhao, Rong Li, Jun Zhao, Qirong ShenAbstract:Long-term vanilla monocropping often results in the occurrence of vanilla Fusarium Wilt Disease, seriously affecting its production all over the world. In the present study, vanilla exhibited significantly less Fusarium Wilt Disease in the soil of a long-term continuously cropped black pepper orchard. The entire fungal communities of bulk and rhizosphere soils between the black pepper-vanilla system (i.e., vanilla cropped in the soil of a continuously cropped black pepper orchard) and vanilla monoculture system were compared through the deep pyrosequencing. The results showed that the black pepper-vanilla system revealed a significantly higher fungal diversity than the vanilla monoculture system in both bulk and rhizosphere soils. The UniFrac-weighted PCoA analysis revealed significant differences in bulk soil fungal community structures between the two cropping systems, and fungal community structures were seriously affected by the vanilla root system. In summary, the black pepper-vanilla system harboured a lower abundance of F. oxysporum in the vanilla rhizosphere soil and increased the putatively plant-beneficial fungal groups such as Trichoderma and Penicillium genus, which could explain the healthy growth of vanilla in the soil of the long-term continuously cropped black pepper field. Thus, cropping vanilla in the soil of continuously cropped black pepper fields for maintaining the vanilla industry is executable and meaningful as an agro-ecological system.
Zongzhuan Shen - One of the best experts on this subject based on the ideXlab platform.
-
fumigation coupled with bio organic fertilizer for the suppression of watermelon fusarium Wilt Disease re shapes the soil microbiome
Applied Soil Ecology, 2019Co-Authors: Chao Xue, Zongzhuan Shen, Yuewen Hao, Weijie Huang, Yao Chong, Wei Ran, Qirong ShenAbstract:Abstract Fusarium Wilt of watermelon caused by Fusarium oxysporums f. sp. niveum (FON) is the most destructive Disease impacting production. Soil fumigation using ammonium bicarbonate coupled with bioorganic fertilizer (BOF) application was conducted in the field in order to suppress Fusarium Wilt Disease. The composition of the soil microbiome after fumigation and at harvest was assessed using MiSeq high throughput sequencing. Soil fumigation succeeded in suppressing Disease incidence, with a drop from 96% to
-
banana fusarium Wilt Disease incidence is influenced by shifts of soil microbial communities under different monoculture spans
Microbial Ecology, 2018Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Ryan C Penton, Xianfu Yuan, Qirong ShenAbstract:The continuous cropping of banana in the same field may result in a serious soil-borne Fusarium Wilt Disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium Wilt Disease in the present study. The results showed that successive cropping of banana was significantly correlated with the Fusarium Wilt Disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium Wilt Disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and Disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower Disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium Wilt Disease.
-
Novel soil fumigation method for suppressing cucumber Fusarium Wilt Disease associated with soil microflora alterations
Applied Soil Ecology, 2016Co-Authors: Zongzhuan Shen, Ruifu Zhang, Li Sun, Xuhui Deng, Qirong ShenAbstract:Fusarium Wilt in cucumber results from continuous cropping and is a serious soil-borne fungal Disease that threatens cucumber production around the world. The application of a novel fumigation agent based on ammonium bicarbonate to the soil as a strategy for controlling Fusarium Wilt and its effects on soil microflora was investigated in a field with serious Disease incidence in this study. Overall, the results showed that fumigation effectively controlled cucumber Fusarium Wilt Disease and significantly increased the total and mean cucumber fruit weight. Real-Time PCR results showed that the total bacterial and fungal numbers in the treatment (LAB) significantly decreased after fumigation and that significantly fewer fungi were observed after harvest (LABOF). The next-generation sequencing of the 16S rRNA and internal transcribed spacer (ITS) genes using MiSeq platform showed that the soil bacterial and fungal community structures in the fumigation treatment were significantly different from the control without fumigation regardless after fumigation or harvest. Compared to the control, higher abundances of Gemmatimonadetes, Verrucomicrobia and Zygomycota, and lower abundance of Ascomycota were observed in the fumigated soils after fumigation and harvest. Furthermore, the abundances of Mortierella and Gp1 were significantly higher. Most importantly, the abundance of Fusarium, which includes the pathogen potentially responsible for cucumber Fusarium Wilt Disease, was significantly lower in the fumigated soils after harvest. Redundancy analysis showed that the fumigated soils were dominated by Ohtaekwangia, Gp6, and Gp4, which were related to the soil total nitrogen (TN), ammonium nitrogen (NH4–N) and nitrate nitrogen (NO3–N) contents. In addition, Penicillium and Pseudaleuria fungi were dominant in the treatment and control, and the control was dominated by Fusarium. In conclusion, the observed Disease suppression due to the novel ecological soil fumigation strategy may be attributed to general suppression resulting from altered soil properties, such as higher soil NH4–N, NO3–N and TON contents, and the alteration of the disturbed soil microflora in a cucumber monoculture system.
-
effect of biofertilizer for suppressing fusarium Wilt Disease of banana as well as enhancing microbial and chemical properties of soil under greenhouse trial
Applied Soil Ecology, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Shutang Zhong, Beibei Wang, Qirong ShenAbstract:Abstract Biofertilizer application has been proposed as a strategy for the management of banana Fusarium Wilt Disease, which has severely decreased banana production. In this study, a 4-season pot experiment was conducted to evaluate the effects of sustainable biofertilizer application at low and high levels on banana Fusarium Wilt Disease suppression, soil chemical properties and microbial communities. The results showed sustainable biofertilizer application effectively controlled the Disease, especially at a high level. Next-generation sequencing of the 16S rRNA and internal transcribed spacer (ITS) genes using the MiSeq platform showed that the soil bacterial and fungal communities in the treatment amended with a high level of biofertilizer (HBIO) were significantly different from a low level biofertilizer treatment (LBIO) or chemical fertilizer control (CF). Moreover, the abundance of Firmicutes and Bacillus was significantly increased, while the abundance of Acidobacteria , Bacteroidetes and Ascomycota was significantly decreased in the HBIO treatment compared with the CF control. Furthermore, the abundance of Fusarium was significantly reduced in the HBIO treatment compared with CF control and was slightly reduced (not significant) compared with the LBIO treatment. Redundancy analysis and Spearman correlation showed that Bacillus , Spartobacteria_genera and TM7_genera dominated in the HBIO treatment and they were positively correlated with the soil pH and the contents of total nitrogen and carbon and available phosphorus, which were negatively correlated with Disease incidence. In conclusion, sustainable biofertilizer application suppressed the Fusarium Wilt Disease might through improving soil chemical condition and manipulating the composition of soil microbial community, including specific enrichment of Firmicutes ( Bacillus ), Anoxybacillus , Spartobacteria_genera , TM7_genera , Cantharellus , Pateramyces and Synchytrium .
-
soils naturally suppressive to banana fusarium Wilt Disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Banana Fusarium Wilt Disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to Disease suppressiveness. Soil bacteria communities from the two banana orchards with excellent Fusarium Disease suppression (suppressive soil) after long-term monoculture and two adjacent banana orchards with serious Fusarium Wilt Disease (conducive soils) were compared using deep 16S RNA barcode pyrosequencing. Compared to the conducive soils within the same field site, higher (P < 0.05) richness and diversity indices were observed in both suppressive soils. Moreover, more operational taxonomic units (OTUs) were observed in the two suppressive soils. Hierarchical cluster analyses showed that bacterial community membership and structure in Disease-suppressive soils differed from Disease-conducive soils. The Acidobacteria phylum was significantly (P < 0.05) elevated, but Bacteroidetes was significantly (P < 0.05) reduced in suppressive soils. The Gp4, Gp5, Chthonomonas, Pseudomonas, and Tumebacillus genera were significantly (P < 0.05) enriched in suppressive soils, but Gp2 was significantly (P < 0.05) reduced in suppressive soils. Furthermore, the enrichment of Gp5 and Pseudomonas as well as the soil physicochemical properties of available phosphorus were significantly (P < 0.05) correlated with Disease suppression. Naturally Disease suppressive soils to banana Fusarium Wilt Disease harbor unique bacterial communities.
Xuanting Jiang - One of the best experts on this subject based on the ideXlab platform.
-
genome and transcriptome analysis of the fungal pathogen fusarium oxysporum f sp cubense causing banana vascular Wilt Disease
PLOS ONE, 2014Co-Authors: Lijia Guo, Lijuan Han, Laying Yang, Huicai Zeng, Dingding Fan, Yabin Zhu, Yue Feng, Guofen Wang, Chunfang Peng, Xuanting JiangAbstract:Background The asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular Wilt Disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced.
-
genome and transcriptome analysis of the fungal pathogen fusarium oxysporum f sp cubense causing banana vascular Wilt Disease
PLOS ONE, 2014Co-Authors: Lijia Guo, Lijuan Han, Laying Yang, Huicai Zeng, Dingding Fan, Yabin Zhu, Yue Feng, Guofen Wang, Chunfang Peng, Xuanting JiangAbstract:Background The asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular Wilt Disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced. Methodology/Principal Findings Genome analysis revealed that the genome structures of race 1 and race 4 isolates were highly syntenic with those of F. oxysporum f. sp. lycopersici strain Fol4287. A large number of putative virulence associated genes were identified in both Foc genomes, including genes putatively involved in root attachment, cell degradation, detoxification of toxin, transport, secondary metabolites biosynthesis and signal transductions. Importantly, relative to the Foc race 1 isolate (Foc1), the Foc race 4 isolate (Foc4) has evolved with some expanded gene families of transporters and transcription factors for transport of toxins and nutrients that may facilitate its ability to adapt to host environments and contribute to pathogenicity to banana. Transcriptome analysis disclosed a significant difference in transcriptional responses between Foc1 and Foc4 at 48 h post inoculation to the banana ‘Brazil’ in comparison with the vegetative growth stage. Of particular note, more virulence-associated genes were up regulated in Foc4 than in Foc1. Several signaling pathways like the mitogen-activated protein kinase Fmk1 mediated invasion growth pathway, the FGA1-mediated G protein signaling pathway and a pathogenicity associated two-component system were activated in Foc4 rather than in Foc1. Together, these differences in gene content and transcription response between Foc1 and Foc4 might account for variation in their virulence during infection of the banana variety ‘Brazil’. Conclusions/Significance Foc genome sequences will facilitate us to identify pathogenicity mechanism involved in the banana vascular Wilt Disease development. These will thus advance us develop effective methods for managing the banana vascular Wilt Disease, including improvement of Disease resistance in banana.
Yunze Ruan - One of the best experts on this subject based on the ideXlab platform.
-
banana fusarium Wilt Disease incidence is influenced by shifts of soil microbial communities under different monoculture spans
Microbial Ecology, 2018Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Ryan C Penton, Xianfu Yuan, Qirong ShenAbstract:The continuous cropping of banana in the same field may result in a serious soil-borne Fusarium Wilt Disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium Wilt Disease in the present study. The results showed that successive cropping of banana was significantly correlated with the Fusarium Wilt Disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium Wilt Disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and Disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower Disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium Wilt Disease.
-
effect of biofertilizer for suppressing fusarium Wilt Disease of banana as well as enhancing microbial and chemical properties of soil under greenhouse trial
Applied Soil Ecology, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Shutang Zhong, Beibei Wang, Qirong ShenAbstract:Abstract Biofertilizer application has been proposed as a strategy for the management of banana Fusarium Wilt Disease, which has severely decreased banana production. In this study, a 4-season pot experiment was conducted to evaluate the effects of sustainable biofertilizer application at low and high levels on banana Fusarium Wilt Disease suppression, soil chemical properties and microbial communities. The results showed sustainable biofertilizer application effectively controlled the Disease, especially at a high level. Next-generation sequencing of the 16S rRNA and internal transcribed spacer (ITS) genes using the MiSeq platform showed that the soil bacterial and fungal communities in the treatment amended with a high level of biofertilizer (HBIO) were significantly different from a low level biofertilizer treatment (LBIO) or chemical fertilizer control (CF). Moreover, the abundance of Firmicutes and Bacillus was significantly increased, while the abundance of Acidobacteria , Bacteroidetes and Ascomycota was significantly decreased in the HBIO treatment compared with the CF control. Furthermore, the abundance of Fusarium was significantly reduced in the HBIO treatment compared with CF control and was slightly reduced (not significant) compared with the LBIO treatment. Redundancy analysis and Spearman correlation showed that Bacillus , Spartobacteria_genera and TM7_genera dominated in the HBIO treatment and they were positively correlated with the soil pH and the contents of total nitrogen and carbon and available phosphorus, which were negatively correlated with Disease incidence. In conclusion, sustainable biofertilizer application suppressed the Fusarium Wilt Disease might through improving soil chemical condition and manipulating the composition of soil microbial community, including specific enrichment of Firmicutes ( Bacillus ), Anoxybacillus , Spartobacteria_genera , TM7_genera , Cantharellus , Pateramyces and Synchytrium .
-
soils naturally suppressive to banana fusarium Wilt Disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Banana Fusarium Wilt Disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to Disease suppressiveness. Soil bacteria communities from the two banana orchards with excellent Fusarium Disease suppression (suppressive soil) after long-term monoculture and two adjacent banana orchards with serious Fusarium Wilt Disease (conducive soils) were compared using deep 16S RNA barcode pyrosequencing. Compared to the conducive soils within the same field site, higher (P < 0.05) richness and diversity indices were observed in both suppressive soils. Moreover, more operational taxonomic units (OTUs) were observed in the two suppressive soils. Hierarchical cluster analyses showed that bacterial community membership and structure in Disease-suppressive soils differed from Disease-conducive soils. The Acidobacteria phylum was significantly (P < 0.05) elevated, but Bacteroidetes was significantly (P < 0.05) reduced in suppressive soils. The Gp4, Gp5, Chthonomonas, Pseudomonas, and Tumebacillus genera were significantly (P < 0.05) enriched in suppressive soils, but Gp2 was significantly (P < 0.05) reduced in suppressive soils. Furthermore, the enrichment of Gp5 and Pseudomonas as well as the soil physicochemical properties of available phosphorus were significantly (P < 0.05) correlated with Disease suppression. Naturally Disease suppressive soils to banana Fusarium Wilt Disease harbor unique bacterial communities.
-
soils naturally suppressive to banana fusarium Wilt Disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Aims Banana Fusarium Wilt Disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to Disease suppressiveness.
-
rhizosphere microbial community manipulated by 2 years of consecutive biofertilizer application associated with banana fusarium Wilt Disease suppression
Biology and Fertility of Soils, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Jian Zhang, Xue Chao, Qirong ShenAbstract:In our previous work, applying biofertilizer containing Bacillus amyloliquefaciens strain NJN-6 to a banana orchard infected by a serious Fusarium Wilt Disease over two consecutive years effectively controlled this soil-borne Disease. In this study, deep pyrosequencing of 16S ribosomal RNA (rRNA) genes and internal transcribed spacer (ITS) sequences was performed to investigate how the composition of rhizosphere microbial community responded to the application of biofertilizer (BIO), pig manure compost (PM), and chemical fertilizer (CF) and to explore the potential correlation between the microbial community composition and the Fusarium Wilt Disease. A total of 104,201 bacterial 16S rRNA genes and 154,953 fungal ITS sequence reads were obtained after basic quality control, and Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, and Ascomycota were the most abundant bacterial and fungal phyla across all samples. Compared with the PM and CF control, the alpha diversity of bacteria significantly (P < 0.05) increased, whereas the value of the fungi was significantly (P < 0.05) reduced following two consecutive years of biofertilizer application. Moreover, the abundance of Acidobacteria (Gp1 and Gp3), Firmicutes, Leptosphaeria, and Phaeosphaeriopsis was significantly (P < 0.05) increased, while the abundance of Proteobacteria and Ascomycota was significantly (P < 0.05) decreased in the BIO treatment. Furthermore, the abundance of Fusarium, a causal pathogen for Fusarium Wilt Disease, was significantly (P < 0.05) reduced in the BIO treatment compared with the CF control and was slightly reduced (not significant) compared with the PM control. Interestingly, the Disease incidence was negatively correlated with the enriched taxa of Acidobacteria (Gp1 and Gp3) and Firmicutes, Leptosphaeria, and Phaeosphaeriopsis but positively correlated with abundance of Proteobacteria, Ascomycota, Fusarium, Cylindrocarpon, Gymnascella, Monographella, Pochonia, and Sakaguchia taxa. The results from this study suggest that 2 years of biofertilizer application manipulated the composition of rhizosphere microbial community and induced the Fusarium suppression by increasing bacterial diversity and potentially stimulating microbial consortia taxa, such as Acidobacteria (Gp1 and Gp3), Firmicutes, Leptosphaeria, and Phaeosphaeriopsis.
Chao Xue - One of the best experts on this subject based on the ideXlab platform.
-
fumigation coupled with bio organic fertilizer for the suppression of watermelon fusarium Wilt Disease re shapes the soil microbiome
Applied Soil Ecology, 2019Co-Authors: Chao Xue, Zongzhuan Shen, Yuewen Hao, Weijie Huang, Yao Chong, Wei Ran, Qirong ShenAbstract:Abstract Fusarium Wilt of watermelon caused by Fusarium oxysporums f. sp. niveum (FON) is the most destructive Disease impacting production. Soil fumigation using ammonium bicarbonate coupled with bioorganic fertilizer (BOF) application was conducted in the field in order to suppress Fusarium Wilt Disease. The composition of the soil microbiome after fumigation and at harvest was assessed using MiSeq high throughput sequencing. Soil fumigation succeeded in suppressing Disease incidence, with a drop from 96% to
-
banana fusarium Wilt Disease incidence is influenced by shifts of soil microbial communities under different monoculture spans
Microbial Ecology, 2018Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Ryan C Penton, Xianfu Yuan, Qirong ShenAbstract:The continuous cropping of banana in the same field may result in a serious soil-borne Fusarium Wilt Disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium Wilt Disease in the present study. The results showed that successive cropping of banana was significantly correlated with the Fusarium Wilt Disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium Wilt Disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and Disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower Disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium Wilt Disease.
-
soils naturally suppressive to banana fusarium Wilt Disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Banana Fusarium Wilt Disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to Disease suppressiveness. Soil bacteria communities from the two banana orchards with excellent Fusarium Disease suppression (suppressive soil) after long-term monoculture and two adjacent banana orchards with serious Fusarium Wilt Disease (conducive soils) were compared using deep 16S RNA barcode pyrosequencing. Compared to the conducive soils within the same field site, higher (P < 0.05) richness and diversity indices were observed in both suppressive soils. Moreover, more operational taxonomic units (OTUs) were observed in the two suppressive soils. Hierarchical cluster analyses showed that bacterial community membership and structure in Disease-suppressive soils differed from Disease-conducive soils. The Acidobacteria phylum was significantly (P < 0.05) elevated, but Bacteroidetes was significantly (P < 0.05) reduced in suppressive soils. The Gp4, Gp5, Chthonomonas, Pseudomonas, and Tumebacillus genera were significantly (P < 0.05) enriched in suppressive soils, but Gp2 was significantly (P < 0.05) reduced in suppressive soils. Furthermore, the enrichment of Gp5 and Pseudomonas as well as the soil physicochemical properties of available phosphorus were significantly (P < 0.05) correlated with Disease suppression. Naturally Disease suppressive soils to banana Fusarium Wilt Disease harbor unique bacterial communities.
-
soils naturally suppressive to banana fusarium Wilt Disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Aims Banana Fusarium Wilt Disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to Disease suppressiveness.
-
deep 16s rrna pyrosequencing reveals a bacterial community associated with banana fusarium Wilt Disease suppression induced by bio organic fertilizer application
PLOS ONE, 2014Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Dongsheng Wang, Jian Zhang, Qirong ShenAbstract:Our previous work demonstrated that application of a bio-organic fertilizer (BIO) to a banana mono-culture orchard with serious Fusarium Wilt Disease effectively decreased the number of soil Fusarium sp. and controlled the soil-borne Disease. Because bacteria are an abundant and diverse group of soil organisms that responds to soil health, deep 16 S rRNA pyrosequencing was employed to characterize the composition of the bacterial community to investigate how it responded to BIO or the application of other common composts and to explore the potential correlation between bacterial community, BIO application and Fusarium Wilt Disease suppression. After basal quality control, 137,646 sequences and 9,388 operational taxonomic units (OTUs) were obtained from the 15 soil samples. Proteobacteria, Acidobacteria, Bacteroidetes, Gemmatimonadetes and Actinobacteria were the most frequent phyla and comprised up to 75.3% of the total sequences. Compared to the other soil samples, BIO-treated soil revealed higher abundances of Gemmatimonadetes and Acidobacteria, while Bacteroidetes were found in lower abundance. Meanwhile, on genus level, higher abundances compared to other treatments were observed for Gemmatimonas and Gp4. Correlation and redundancy analysis showed that the abundance of Gemmatimonas and Sphingomonas and the soil total nitrogen and ammonium nitrogen content were higher after BIO application, and they were all positively correlated with Disease suppression. Cumulatively, the reduced Fusarium Wilt Disease incidence that was seen after BIO was applied for 1-year might be attributed to the general suppression based on a shift within the bacteria soil community, including specific enrichment of Gemmatimonas and Sphingomonas.
