The Experts below are selected from a list of 33807 Experts worldwide ranked by ideXlab platform
Jeffrey S Rubin - One of the best experts on this subject based on the ideXlab platform.
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Existence of the canonical Wnt Signaling Pathway in the human trabecular meshwork.
Investigative Ophthalmology & Visual Science, 2012Co-Authors: Weiming Mao, Wanheng Wang, Sean Silverman, Robert J Wordinger, Jeffrey S Rubin, Iokhou Pang, J. Cameron Millar, Yang Liu, F. ClarkAbstract:Purpose We previously discovered elevated levels of secreted frizzled-related protein 1 (sFRP1), the Wnt Signaling Pathway inhibitor, in the glaucomatous trabecular meshwork (GTM), and found that key canonical Wnt Signaling Pathway genes are expressed in the trabecular meshwork (TM). The purpose of our study was to determine whether a functional canonical Wnt Signaling Pathway exists in the human TM (HTM). Methods Western immunoblotting and/or immunofluorescent microscopy were used to study β-catenin translocation as well as the actin cytoskeleton in transformed and primary HTM cells. A TCF/LEF luciferase assay was used to study functional canonical Wnt Signaling, which was confirmed further by Wnt3a-induced expression of a Pathway target gene, AXIN2, via quantitative PCR. Intravitreal injection of an Ad5 adenovirus expressing Dickkopf-related protein-1 (DKK1) was used to study the in vivo effect of canonical Wnt Signaling on IOP in mice. Results Wnt3a induced β-catenin translocation in the HTM, which was blocked by co-treatment with sFRP1. Similarly, Wnt3a enhanced luciferase levels in TCF/LEF luciferase assays, which also were blocked by sFRP1. Furthermore, AXIN2 expression was elevated significantly by Wnt3a. However, neither Wnt3a nor sFRP1 affected actin cytoskeleton organization, which theoretically could be regulated by noncanonical Wnt Signaling in HTM cells. Exogenous DKK1, a specific inhibitor for the canonical Wnt Signaling Pathway, or sFRP1 elevated mouse IOP to equivalent levels. Conclusions There is a canonical Wnt Signaling Pathway in the TM, and this canonical Wnt Pathway, but not the noncanonical Wnt Signaling Pathway, regulates IOP.
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existence of the canonical Wnt Signaling Pathway in the human trabecular meshwork
Investigative Ophthalmology & Visual Science, 2012Co-Authors: Cameron J Millar, Wanheng Wang, Sean Silverman, Robert J Wordinger, Jeffrey S Rubin, Iokhou Pang, Abbot F ClarkAbstract:We previously discovered elevated levels of secreted frizzled-related protein 1 (sFRP1), the Wnt Signaling Pathway inhibitor, in the glaucomatous trabecular meshwork (GTM), and found that key canonical Wnt Signaling Pathway genes are expressed in the trabecular meshwork (TM). The purpose of our study was to determine whether a functional canonical Wnt Signaling Pathway exists in the human TM (HTM).Western immunoblotting and/or immunofluorescent microscopy were used to study β-catenin translocation as well as the actin cytoskeleton in transformed and primary HTM cells. A TCF/LEF luciferase assay was used to study functional canonical Wnt Signaling, which was confirmed further by Wnt3a-induced expression of a Pathway target gene, AXIN2, via quantitative PCR. Intravitreal injection of an Ad5 adenovirus expressing Dickkopf-related protein-1 (DKK1) was used to study the in vivo effect of canonical Wnt Signaling on IOP in mice.Wnt3a induced β-catenin translocation in the HTM, which was blocked by co-treatment with sFRP1. Similarly, Wnt3a enhanced luciferase levels in TCF/LEF luciferase assays, which also were blocked by sFRP1. Furthermore, AXIN2 expression was elevated significantly by Wnt3a. However, neither Wnt3a nor sFRP1 affected actin cytoskeleton organization, which theoretically could be regulated by noncanonical Wnt Signaling in HTM cells. Exogenous DKK1, a specific inhibitor for the canonical Wnt Signaling Pathway, or sFRP1 elevated mouse IOP to equivalent levels.There is a canonical Wnt Signaling Pathway in the TM, and this canonical Wnt Pathway, but not the noncanonical Wnt Signaling Pathway, regulates IOP.
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akt participation in the Wnt Signaling Pathway through dishevelled
Journal of Biological Chemistry, 2001Co-Authors: Shinya Fukumoto, Chungming Hsieh, Koji Maemura, Matthew D Layne, Shawfang Yet, Kyunghan Lee, Takashi Matsui, Anthony Rosenzweig, William G Taylor, Jeffrey S RubinAbstract:Abstract Inactivation of glycogen synthase kinase 3β (GSK3β) and the resulting stabilization of free β-catenin are critical steps in the activation of Wnt target genes. While Akt regulates GSK3α/β in the phosphatidylinositide 3-OH kinase Signaling Pathway, its role in Wnt Signaling is unknown. Here we report that expression of Wnt or Dishevelled (Dvl) increased Akt activity. Activated Akt bound to the Axin-GSK3β complex in the presence of Dvl, phosphorylated GSK3β and increased free β-catenin levels. Furthermore, in Wnt-overexpressing PC12 cells, dominant-negative Akt decreased free β-catenin and derepressed nerve growth factor-induced differentiation. Therefore, Akt acts in association with Dvl as an important regulator of the Wnt Signaling Pathway.
Abbot F Clark - One of the best experts on this subject based on the ideXlab platform.
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existence of the canonical Wnt Signaling Pathway in the human trabecular meshwork
Investigative Ophthalmology & Visual Science, 2012Co-Authors: Cameron J Millar, Wanheng Wang, Sean Silverman, Robert J Wordinger, Jeffrey S Rubin, Iokhou Pang, Abbot F ClarkAbstract:We previously discovered elevated levels of secreted frizzled-related protein 1 (sFRP1), the Wnt Signaling Pathway inhibitor, in the glaucomatous trabecular meshwork (GTM), and found that key canonical Wnt Signaling Pathway genes are expressed in the trabecular meshwork (TM). The purpose of our study was to determine whether a functional canonical Wnt Signaling Pathway exists in the human TM (HTM).Western immunoblotting and/or immunofluorescent microscopy were used to study β-catenin translocation as well as the actin cytoskeleton in transformed and primary HTM cells. A TCF/LEF luciferase assay was used to study functional canonical Wnt Signaling, which was confirmed further by Wnt3a-induced expression of a Pathway target gene, AXIN2, via quantitative PCR. Intravitreal injection of an Ad5 adenovirus expressing Dickkopf-related protein-1 (DKK1) was used to study the in vivo effect of canonical Wnt Signaling on IOP in mice.Wnt3a induced β-catenin translocation in the HTM, which was blocked by co-treatment with sFRP1. Similarly, Wnt3a enhanced luciferase levels in TCF/LEF luciferase assays, which also were blocked by sFRP1. Furthermore, AXIN2 expression was elevated significantly by Wnt3a. However, neither Wnt3a nor sFRP1 affected actin cytoskeleton organization, which theoretically could be regulated by noncanonical Wnt Signaling in HTM cells. Exogenous DKK1, a specific inhibitor for the canonical Wnt Signaling Pathway, or sFRP1 elevated mouse IOP to equivalent levels.There is a canonical Wnt Signaling Pathway in the TM, and this canonical Wnt Pathway, but not the noncanonical Wnt Signaling Pathway, regulates IOP.
Rana R Mckay - One of the best experts on this subject based on the ideXlab platform.
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characterization of Wnt Signaling Pathway wsp aberrations in advanced prostate cancer
Journal of Clinical Oncology, 2020Co-Authors: Fady Ghali, Devin Patel, Christina Jamieson, Kellogg J Parsons, Rana R MckayAbstract:203Background: Aberrations in Wnt Signaling Pathway (WSP) are implicated in disease progression and resistance of multiple malignancies including prostate cancer (PCa). We sought to characterize th...
Cameron J Millar - One of the best experts on this subject based on the ideXlab platform.
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existence of the canonical Wnt Signaling Pathway in the human trabecular meshwork
Investigative Ophthalmology & Visual Science, 2012Co-Authors: Cameron J Millar, Wanheng Wang, Sean Silverman, Robert J Wordinger, Jeffrey S Rubin, Iokhou Pang, Abbot F ClarkAbstract:We previously discovered elevated levels of secreted frizzled-related protein 1 (sFRP1), the Wnt Signaling Pathway inhibitor, in the glaucomatous trabecular meshwork (GTM), and found that key canonical Wnt Signaling Pathway genes are expressed in the trabecular meshwork (TM). The purpose of our study was to determine whether a functional canonical Wnt Signaling Pathway exists in the human TM (HTM).Western immunoblotting and/or immunofluorescent microscopy were used to study β-catenin translocation as well as the actin cytoskeleton in transformed and primary HTM cells. A TCF/LEF luciferase assay was used to study functional canonical Wnt Signaling, which was confirmed further by Wnt3a-induced expression of a Pathway target gene, AXIN2, via quantitative PCR. Intravitreal injection of an Ad5 adenovirus expressing Dickkopf-related protein-1 (DKK1) was used to study the in vivo effect of canonical Wnt Signaling on IOP in mice.Wnt3a induced β-catenin translocation in the HTM, which was blocked by co-treatment with sFRP1. Similarly, Wnt3a enhanced luciferase levels in TCF/LEF luciferase assays, which also were blocked by sFRP1. Furthermore, AXIN2 expression was elevated significantly by Wnt3a. However, neither Wnt3a nor sFRP1 affected actin cytoskeleton organization, which theoretically could be regulated by noncanonical Wnt Signaling in HTM cells. Exogenous DKK1, a specific inhibitor for the canonical Wnt Signaling Pathway, or sFRP1 elevated mouse IOP to equivalent levels.There is a canonical Wnt Signaling Pathway in the TM, and this canonical Wnt Pathway, but not the noncanonical Wnt Signaling Pathway, regulates IOP.
Wu Shu-guang - One of the best experts on this subject based on the ideXlab platform.
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Effects on expression of Wnt Signaling Pathway inhibitor FrpHE
Journal of Practical Oncology, 2007Co-Authors: Wu Shu-guangAbstract:Objective To investigate the effects of Wnt Signaling Pathway inhibitor FrpHE.Methods Human hepatocarcinoma Hep3B cells were transfected with a replication-defective advenovirus encoding(Adp53),and Wnt Signaling Pathway function was evaluated with changes with key factor β-catenin.FrpHE mRNA expression was detected by reverse transfection(RT)-PCR,β-catenin and Adp53 transgene expression were measured by fluorescence-activated cell sorting(FACS).Results FrpHE mRNA in Hep3B cells was initially potentiated by transfection with Adp53 after 20 hours,and the peak expression was seen after 32 hours.Dose-effect studies revealed that Adp53 in dose range of 0.05,0.5,5 and 50 pfu/cell could promote the expression of FrpHE mRNA,with the highest expression at the dose of 50 pfu/cell.β-catenin positive cells percent and fluorescence intensity was decreased,along with the transfection time and dosage gain.Consultions Ectogentic p53 can promote mRNA expression of Wnt Signaling Pathway inhibitor FrpHE in Hep3B,and further suppress effect of Wnt Signaling Pathway.
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Effect on expression of Wnt Signaling Pathway inhibitor Dickkopf-1
Chinese Journal of Hospital Pharmacy, 2007Co-Authors: Wu Shu-guangAbstract:OBJECTIVE To investigate the effect of Wnt Signaling Pathway inhibitor DKK-1.METHODS Human hepatocarcinoma Hep3B cells were transfected with a replication-defective advenovirus encoding(Adp53),and Wnt Signaling Pathway function changes with key factor β-catenin.DKK-1 mRNA expression detected by reverse transfection(RT)-PCR,β-catenin and Adp53 transgene expression was measured by fluorescence-activated cell sorting(FACS).RESULTS DKK-1 mRNA in Hep3B cells was initially potentiated by transfection with Adp53 after 20 h,and the peak expression was seen at 32 h.Dose-effect studies revealed that Adp53 in dose range of 0.5,5 and 50 pfu/cell could promote the expression of DKK-1 mRNA,with the highest expression at the dose of 50 pfu/cell.β-catenin,positive cells percent and fluorescence intensity was descent,along with increased transfection time and dosage gain.CONCLUSION Ectogentic p53 promotes mRNA expression of Wnt Signaling Pathway inhibitor DKK-1 in Hep3B and suppress Wnt Signaling Pathway.
