The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
B. Bharathiraja - One of the best experts on this subject based on the ideXlab platform.
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Bioethanol production from Woody Stem Prosopis juliflora using thermo tolerant yeast Kluyveromyces marxianus and its kinetics studies.
Bioresource technology, 2019Co-Authors: S. Sivarathnakumar, Gurunathan Baskar, R. Praveenkumar, J. Jayamuthunagai, I. Aberna Ebenezer Selvakumari, B. BharathirajaAbstract:In the present study, Kluyveromyces marxianus was utilized to study the batch fermentation kinetics of biomass production, substrate utilization and bioethanol production from Woody Stem Prosopis juliflora. The pre-treated substrate was subjected to Simultaneous Saccharification and Fermentation (SSF) under optimised conditions of pH (4.9), temperature (41 °C), substrate concentration 5% (w/v), inoculum concentration 3% (v/v) and the maximum concentration of bioethanol was found to be 21.45 g/l. The experimental data thus obtained from cell growth, substrate utilization and product formation are employed in the determination of kinetic parameters. Biological models such as Logistic model, Hinshelwood model were used for microbial growth and substrate utilization kinetics respectively. In case of product kinetics, Leudking-Piret plot, Gompertz model and Modified Gompertz model were utilised. Based on these models, kinetic parameters like maximum specific growth rate (µm), saturation constant (Ks), growth associated (α), non-growth associated (β) and yield coefficients (YX/S, YP/S) were estimated.
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Simultaneous saccharification and fermentation of Woody Stem Prosopis juliflora by Zymomonas mobilis for the production of cellulosic ethanol
International Journal of Materials and Product Technology, 2017Co-Authors: S. Sivarathnakumar, Gurunathan Baskar, R. Praveenkumar, B. BharathirajaAbstract:Pre-treatment of lignocellulosic biomass is found to be a pivotal task in the production of biofuels. In this study, Woody Stem Prosopis juliflora initially was subjected to auto hydrolysis followed by nitric acid treatment (3%(v/v)) and sonication. Further a combination of auto hydrolysis coupled with nitric acid treatment (3%(v/v)) followed by sonication was carried out. The outcome of these two main processes was to ascertain the maximum production of cellulose and hemicelluloses from lignocellulosic biomass and use it for simultaneous saccharification and fermentation (SSF) with commercially available enzyme and gram negative bacteria Zymomonas mobilis in the production of cellulosic ethanol. The process parameters such as pH, temperature, substrate concentration and inoculum volume in the production of cellulosic ethanol were investigated. The maximum bioethanol concentration produced by fermentation of Woody Stem Prosopis juliflora using Zymomonas mobilis was found to be 16.40 g/l.
S. Sivarathnakumar - One of the best experts on this subject based on the ideXlab platform.
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Bioethanol production from Woody Stem Prosopis juliflora using thermo tolerant yeast Kluyveromyces marxianus and its kinetics studies.
Bioresource technology, 2019Co-Authors: S. Sivarathnakumar, Gurunathan Baskar, R. Praveenkumar, J. Jayamuthunagai, I. Aberna Ebenezer Selvakumari, B. BharathirajaAbstract:In the present study, Kluyveromyces marxianus was utilized to study the batch fermentation kinetics of biomass production, substrate utilization and bioethanol production from Woody Stem Prosopis juliflora. The pre-treated substrate was subjected to Simultaneous Saccharification and Fermentation (SSF) under optimised conditions of pH (4.9), temperature (41 °C), substrate concentration 5% (w/v), inoculum concentration 3% (v/v) and the maximum concentration of bioethanol was found to be 21.45 g/l. The experimental data thus obtained from cell growth, substrate utilization and product formation are employed in the determination of kinetic parameters. Biological models such as Logistic model, Hinshelwood model were used for microbial growth and substrate utilization kinetics respectively. In case of product kinetics, Leudking-Piret plot, Gompertz model and Modified Gompertz model were utilised. Based on these models, kinetic parameters like maximum specific growth rate (µm), saturation constant (Ks), growth associated (α), non-growth associated (β) and yield coefficients (YX/S, YP/S) were estimated.
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Simultaneous saccharification and fermentation of Woody Stem Prosopis juliflora by Zymomonas mobilis for the production of cellulosic ethanol
International Journal of Materials and Product Technology, 2017Co-Authors: S. Sivarathnakumar, Gurunathan Baskar, R. Praveenkumar, B. BharathirajaAbstract:Pre-treatment of lignocellulosic biomass is found to be a pivotal task in the production of biofuels. In this study, Woody Stem Prosopis juliflora initially was subjected to auto hydrolysis followed by nitric acid treatment (3%(v/v)) and sonication. Further a combination of auto hydrolysis coupled with nitric acid treatment (3%(v/v)) followed by sonication was carried out. The outcome of these two main processes was to ascertain the maximum production of cellulose and hemicelluloses from lignocellulosic biomass and use it for simultaneous saccharification and fermentation (SSF) with commercially available enzyme and gram negative bacteria Zymomonas mobilis in the production of cellulosic ethanol. The process parameters such as pH, temperature, substrate concentration and inoculum volume in the production of cellulosic ethanol were investigated. The maximum bioethanol concentration produced by fermentation of Woody Stem Prosopis juliflora using Zymomonas mobilis was found to be 16.40 g/l.
Sarah J. Cookson - One of the best experts on this subject based on the ideXlab platform.
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Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine.
BMC plant biology, 2019Co-Authors: Duyên Prodhomme, Céline Franc, Ghislaine Hilbert, Gilles De Revel, Tristan Richard, Nathalie Ollat, Josep Valls Fonayet, Cyril Hévin, Sarah J. CooksonAbstract:Grafting with rootstocks is essential for the culture of many perennial fruit crops and is increasing being used in the production of annual fruits and vegetables. Our previous work based on microarrays showed that transcripts encoding enzymes of both primary and secondary metabolism were differentially expressed during graft union formation in both homo-grafts (a genotype grafted with itself) and hetero-grafts (two different genotypes grafted together). The aim of this study was to profile primary and secondary metabolites, and quantify the activity of phenylalanine ammonia lyase (PAL) and neutral invertase (NI) in the scion and rootstock tissues and the graft interface of homo and hetero-grafts of grapevine 1 month after grafting. Table-top grafting was done on over-wintering Stems (canes) of grapevine and the graft interface tissues (containing some Woody Stem tissues and callus) were compared to the surrounding rootstock and scion tissues. The objective was to identify compounds involved in graft union formation and hetero-grafting responses. A total of 54 compounds from primary and secondary metabolism (19 amino acids, five primary and 30 secondary compounds metabolites) and the activity of two enzymes were measured. The graft interface was associated with an increase in the accumulation of the branched-chain amino acids, basic amino acids, certain stilbene compounds and higher PAL and NI activity in comparison to the surrounding Woody Stem tissues. Some amino acids and stilbenes were identified as being accumulated differently between the graft interfaces of the scion/rootstock combinations in a manner which was unrelated to their concentrations in the surrounding Woody Stem tissues. This study revealed the modification of primary metabolism to support callus cell formation and the stimulation of stilbene synthesis at the graft interface, and how these processes are modified by hetero-grafting. Knowledge of the metabolites and/or enzymes required for successful graft union formation offer us the potential to identify markers that could be used by nurseries and researchers for selection and breeding purposes.
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Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine
BMC Plant Biology, 2019Co-Authors: Duyên Prodhomme, Josep Valls Fonayet, Céline Franc, Ghislaine Hilbert, Gilles De Revel, Tristan Richard, Nathalie Ollat, Sarah J. CooksonAbstract:BACKGROUND: Grafting with rootstocks is essential for the culture of many perennial fruit crops and is increasing being used in the production of annual fruits and vegetables. Our previous work based on microarrays showed that transcripts encoding enzymes of both primary and secondary metabolism were differentially expressed during graft union formation in both homo-grafts (a genotype grafted with itself) and hetero-grafts (two different genotypes grafted together). The aim of this study was to profile primary and secondary metabolites, and quantify the activity of phenylalanine ammonia lyase (PAL) and neutral invertase (NI) in the scion and rootstock tissues and the graft interface of homo and hetero-grafts of grapevine 1 month after grafting. Table-top grafting was done on over-wintering Stems (canes) of grapevine and the graft interface tissues (containing some Woody Stem tissues and callus) were compared to the surrounding rootstock and scion tissues. The objective was to identify compounds involved in graft union formation and hetero-grafting responses. RESULTS: A total of 54 compounds from primary and secondary metabolism (19 amino acids, five primary and 30 secondary compounds metabolites) and the activity of two enzymes were measured. The graft interface was associated with an increase in the accumulation of the branched-chain amino acids, basic amino acids, certain stilbene compounds and higher PAL and NI activity in comparison to the surrounding Woody Stem tissues. Some amino acids and stilbenes were identified as being accumulated differently between the graft interfaces of the scion/rootstock combinations in a manner which was unrelated to their concentrations in the surrounding Woody Stem tissues. CONCLUSIONS: This study revealed the modification of primary metabolism to support callus cell formation and the stimulation of stilbene synthesis at the graft interface, and how these processes are modified by hetero-grafting. Knowledge of the metabolites and/or enzymes required for successful graft union formation offer us the potential to identify markers that could be used by nurseries and researchers for selection and breeding purposes.
Gurunathan Baskar - One of the best experts on this subject based on the ideXlab platform.
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Bioethanol production from Woody Stem Prosopis juliflora using thermo tolerant yeast Kluyveromyces marxianus and its kinetics studies.
Bioresource technology, 2019Co-Authors: S. Sivarathnakumar, Gurunathan Baskar, R. Praveenkumar, J. Jayamuthunagai, I. Aberna Ebenezer Selvakumari, B. BharathirajaAbstract:In the present study, Kluyveromyces marxianus was utilized to study the batch fermentation kinetics of biomass production, substrate utilization and bioethanol production from Woody Stem Prosopis juliflora. The pre-treated substrate was subjected to Simultaneous Saccharification and Fermentation (SSF) under optimised conditions of pH (4.9), temperature (41 °C), substrate concentration 5% (w/v), inoculum concentration 3% (v/v) and the maximum concentration of bioethanol was found to be 21.45 g/l. The experimental data thus obtained from cell growth, substrate utilization and product formation are employed in the determination of kinetic parameters. Biological models such as Logistic model, Hinshelwood model were used for microbial growth and substrate utilization kinetics respectively. In case of product kinetics, Leudking-Piret plot, Gompertz model and Modified Gompertz model were utilised. Based on these models, kinetic parameters like maximum specific growth rate (µm), saturation constant (Ks), growth associated (α), non-growth associated (β) and yield coefficients (YX/S, YP/S) were estimated.
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Simultaneous saccharification and fermentation of Woody Stem Prosopis juliflora by Zymomonas mobilis for the production of cellulosic ethanol
International Journal of Materials and Product Technology, 2017Co-Authors: S. Sivarathnakumar, Gurunathan Baskar, R. Praveenkumar, B. BharathirajaAbstract:Pre-treatment of lignocellulosic biomass is found to be a pivotal task in the production of biofuels. In this study, Woody Stem Prosopis juliflora initially was subjected to auto hydrolysis followed by nitric acid treatment (3%(v/v)) and sonication. Further a combination of auto hydrolysis coupled with nitric acid treatment (3%(v/v)) followed by sonication was carried out. The outcome of these two main processes was to ascertain the maximum production of cellulose and hemicelluloses from lignocellulosic biomass and use it for simultaneous saccharification and fermentation (SSF) with commercially available enzyme and gram negative bacteria Zymomonas mobilis in the production of cellulosic ethanol. The process parameters such as pH, temperature, substrate concentration and inoculum volume in the production of cellulosic ethanol were investigated. The maximum bioethanol concentration produced by fermentation of Woody Stem Prosopis juliflora using Zymomonas mobilis was found to be 16.40 g/l.
R. Praveenkumar - One of the best experts on this subject based on the ideXlab platform.
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Bioethanol production from Woody Stem Prosopis juliflora using thermo tolerant yeast Kluyveromyces marxianus and its kinetics studies.
Bioresource technology, 2019Co-Authors: S. Sivarathnakumar, Gurunathan Baskar, R. Praveenkumar, J. Jayamuthunagai, I. Aberna Ebenezer Selvakumari, B. BharathirajaAbstract:In the present study, Kluyveromyces marxianus was utilized to study the batch fermentation kinetics of biomass production, substrate utilization and bioethanol production from Woody Stem Prosopis juliflora. The pre-treated substrate was subjected to Simultaneous Saccharification and Fermentation (SSF) under optimised conditions of pH (4.9), temperature (41 °C), substrate concentration 5% (w/v), inoculum concentration 3% (v/v) and the maximum concentration of bioethanol was found to be 21.45 g/l. The experimental data thus obtained from cell growth, substrate utilization and product formation are employed in the determination of kinetic parameters. Biological models such as Logistic model, Hinshelwood model were used for microbial growth and substrate utilization kinetics respectively. In case of product kinetics, Leudking-Piret plot, Gompertz model and Modified Gompertz model were utilised. Based on these models, kinetic parameters like maximum specific growth rate (µm), saturation constant (Ks), growth associated (α), non-growth associated (β) and yield coefficients (YX/S, YP/S) were estimated.
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Simultaneous saccharification and fermentation of Woody Stem Prosopis juliflora by Zymomonas mobilis for the production of cellulosic ethanol
International Journal of Materials and Product Technology, 2017Co-Authors: S. Sivarathnakumar, Gurunathan Baskar, R. Praveenkumar, B. BharathirajaAbstract:Pre-treatment of lignocellulosic biomass is found to be a pivotal task in the production of biofuels. In this study, Woody Stem Prosopis juliflora initially was subjected to auto hydrolysis followed by nitric acid treatment (3%(v/v)) and sonication. Further a combination of auto hydrolysis coupled with nitric acid treatment (3%(v/v)) followed by sonication was carried out. The outcome of these two main processes was to ascertain the maximum production of cellulose and hemicelluloses from lignocellulosic biomass and use it for simultaneous saccharification and fermentation (SSF) with commercially available enzyme and gram negative bacteria Zymomonas mobilis in the production of cellulosic ethanol. The process parameters such as pH, temperature, substrate concentration and inoculum volume in the production of cellulosic ethanol were investigated. The maximum bioethanol concentration produced by fermentation of Woody Stem Prosopis juliflora using Zymomonas mobilis was found to be 16.40 g/l.
