The Experts below are selected from a list of 162 Experts worldwide ranked by ideXlab platform
Ning Wang - One of the best experts on this subject based on the ideXlab platform.
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Structural Characterization and Association of Ovine Dickkopf-1 Gene with Wool Production and Quality Traits in Chinese Merino.
Genes, 2017Co-Authors: En-guang Rong, Hua Yang, Zhipeng Wang, Xiaohong Yan, Yang Jing, Ma Guangwei, Ning WangAbstract:Dickkopf-1 (DKK1) is an inhibitor of canonical Wnt signaling pathway and regulates hair follicle morphogenesis and cycling. To investigate the potential involvement of DKK1 in Wool Production and quality traits, we characterized the genomic structure of ovine DKK1, performed polymorphism detection and association analysis of ovine DKK1 with Wool Production and quality traits in Chinese Merino. Our results showed that ovine DKK1 consists of four exons and three introns, which encodes a protein of 262 amino acids. The coding sequence of ovine DKK1 and its deduced amino acid sequence were highly conserved in mammals. Eleven single nucleotide polymorphisms (SNPs) were identified within the ovine DKK1 genomic region. Gene-wide association analysis showed that SNP5 was significantly associated with mean fiber diameter (MFD) in the B (selected for long Wool fiber and high-quality Wool), PW (selected for high reproductive capacity, high clean Wool yield and high-quality Wool) and U (selected for long Wool fiber with good uniformity, high Wool yield and lower fiber diameter) strains (p < 4.55 × 10-3 = 0.05/11). Single Nucleotide Polymorphisms wide association analysis showed that SNP8 was significantly associated with MFD in A strain and fleece weight in A (selected for large body size), PM (selected for large body size, high reproductive capacity and high meat yield) and SF (selected for mean fiber diameter less than 18 μm and Wool fiber length between 5 and 9 cm) strains (p < 0.05), SNP9 was significantly associated with curvature in B and U strains (p < 0.05) and SNP10 was significantly associated with coefficient of variation of fiber diameter in A, PW and PM strains and standard deviation of fiber diameter in A and PM strains (p < 0.05). The haplotypes derived from these 11 identified SNPs were significantly associated with MFD (p < 0.05). In conclusion, our results suggest that DKK1 may be a major gene controlling Wool Production and quality traits, also the identified SNPs (SNPs5, 8, 9 and 10) might be used as potential molecular markers for improving sheep Wool Production and quality in sheep breeding.
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association of methionine synthase gene polymorphisms with Wool Production and quality traits in chinese merino population
Journal of Animal Science, 2015Co-Authors: En-guang Rong, Zhipeng Wang, Huanming Yang, Zhiwei Zhang, Xiaohong Yan, Ning WangAbstract:Methionine synthase (MTR) plays a crucial role in maintaining homeostasis of intracellular methionine, folate, and homocysteine, and its activity correlates with DNA methylation in many mammalian tissues. Our previous genomewide association study identified that 1 SNP located in the gene was associated with several Wool Production and quality traits in Chinese Merino. To confirm the potential involvement of the gene in sheep Wool Production and quality traits, we performed sheep tissue expression profiling, SNP detection, and association analysis with sheep Wool Production and quality traits. The semiquantitative reverse transcription PCR analysis showed that the gene was differentially expressed in skin from Merino and Kazak sheep. The sequencing analysis identified a total of 13 SNP in the gene from Chinese Merino sheep. Comparison of the allele frequencies revealed that these 13 identified SNP were significantly different among the 6 tested Chinese Merino strains ( < 0.001). Linkage disequilibrium analysis showed that SNP 3 to 11 were strongly linked in a single haplotype block in the tested population. Association analysis showed that SNP 2 to 11 were significantly associated with the average Wool fiber diameter and the fineness SD and that SNP 4 to 11 were significantly associated with the CV of fiber diameter trait ( < 0.05). Single nucleotide polymorphism 2 and SNP 5 to 12 were weakly associated with Wool crimp. Similarly, the haplotypes derived from these 13 identified SNP were also significantly associated with the average Wool fiber diameter, fineness SD, and the CV of fiber diameter ( < 0.05). Our results suggest that is a candidate gene for sheep Wool Production and quality traits, and the identified SNP might be used in sheep breeding.
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genome wide association study for Wool Production traits in a chinese merino sheep population
PLOS ONE, 2014Co-Authors: Zhipeng Wang, Hua Yang, En-guang Rong, Shouzhi Wang, Hui Zhang, Wenyu Pei, Ning WangAbstract:Genome-wide association studies (GWAS) provide a powerful approach for identifying quantitative trait loci without prior knowledge of location or function. To identify loci associated with Wool Production traits, we performed a genome-wide association study on a total of 765 Chinese Merino sheep (JunKen type) genotyped with 50 K single nucleotide polymorphisms (SNPs). In the present study, five Wool Production traits were examined: fiber diameter, fiber diameter coefficient of variation, fineness dispersion, staple length and crimp. We detected 28 genome-wide significant SNPs for fiber diameter, fiber diameter coefficient of variation, fineness dispersion, and crimp trait in the Chinese Merino sheep. About 43% of the significant SNP markers were located within known or predicted genes, including YWHAZ, KRTCAP3, TSPEAR, PIK3R4, KIF16B, PTPN3, GPRC5A, DDX47, TCF9, TPTE2, EPHA5 and NBEA genes. Our results not only confirm the results of previous reports, but also provide a suite of novel SNP markers and candidate genes associated with Wool traits. Our findings will be useful for exploring the genetic control of Wool traits in sheep.
Serdar Çulha - One of the best experts on this subject based on the ideXlab platform.
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Investigation of boron usability in rock Wool Production
Construction and Building Materials, 2020Co-Authors: Abdulkerim Yörükoğlu, Fatih Akkurt, Serdar ÇulhaAbstract:Abstract The effect of boron addition on basalt and basalt-dolomite mixtures was tested for rock Wool Production. The melting temperatures and the viscosities of the melts were determined and SEM and EDS analysis on some samples were done to find out the effect of boron addition. It was observed that boron addition lowered the melting temperatures noticeably, slight increases in the viscosities were at acceptable levels and no negative structural changes were caused. It was concluded that boron could be added for the rock Wool Production and the quantity to be used could be adjusted considering the melting temperature and the viscosity value.
O Kamstrup - One of the best experts on this subject based on the ideXlab platform.
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Simulation of past exposure in slag Wool Production.
The Annals of occupational hygiene, 1993Co-Authors: B Fallentin, O KamstrupAbstract:A survey of the working conditions at a Danish slag Wool Production factory during the early technological phase in the 1940s is presented. No exposure data, however, are available for that period. So, a full-scale simulation of the past Production of slag Wool has been performed. Air monitoring was carried out in the working area around the cupola furnace. The aim was to measure exposure to air pollutants other than fibres. Such exposure might have confounded a possible association between lung cancer and exposure to fibres, in the early technological phase of slag Wool Production. The simulation experiment demonstrated exposure to PAH, a known lung carcinogen. The effect of other concurrent exposures is difficult to assess. Time-weighted average concentrations of particulate material ranged between 12.9 and 49.1 mg m-3 at the upper decks around the cupola. Corresponding concentrations of the dominant metals zinc and lead were 4.4-22.7 mg Zn m-3 and 0.9-4.7 mg Pb m-3. Significant concentrations of PAH up to 269 micrograms PAH m-3 (4 micrograms BaP m-3) occurred during ignition of the cupola furnace. The carbon monoxide level reached 270 ppm also during ignition.
Zhipeng Wang - One of the best experts on this subject based on the ideXlab platform.
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Structural Characterization and Association of Ovine Dickkopf-1 Gene with Wool Production and Quality Traits in Chinese Merino.
Genes, 2017Co-Authors: En-guang Rong, Hua Yang, Zhipeng Wang, Xiaohong Yan, Yang Jing, Ma Guangwei, Ning WangAbstract:Dickkopf-1 (DKK1) is an inhibitor of canonical Wnt signaling pathway and regulates hair follicle morphogenesis and cycling. To investigate the potential involvement of DKK1 in Wool Production and quality traits, we characterized the genomic structure of ovine DKK1, performed polymorphism detection and association analysis of ovine DKK1 with Wool Production and quality traits in Chinese Merino. Our results showed that ovine DKK1 consists of four exons and three introns, which encodes a protein of 262 amino acids. The coding sequence of ovine DKK1 and its deduced amino acid sequence were highly conserved in mammals. Eleven single nucleotide polymorphisms (SNPs) were identified within the ovine DKK1 genomic region. Gene-wide association analysis showed that SNP5 was significantly associated with mean fiber diameter (MFD) in the B (selected for long Wool fiber and high-quality Wool), PW (selected for high reproductive capacity, high clean Wool yield and high-quality Wool) and U (selected for long Wool fiber with good uniformity, high Wool yield and lower fiber diameter) strains (p < 4.55 × 10-3 = 0.05/11). Single Nucleotide Polymorphisms wide association analysis showed that SNP8 was significantly associated with MFD in A strain and fleece weight in A (selected for large body size), PM (selected for large body size, high reproductive capacity and high meat yield) and SF (selected for mean fiber diameter less than 18 μm and Wool fiber length between 5 and 9 cm) strains (p < 0.05), SNP9 was significantly associated with curvature in B and U strains (p < 0.05) and SNP10 was significantly associated with coefficient of variation of fiber diameter in A, PW and PM strains and standard deviation of fiber diameter in A and PM strains (p < 0.05). The haplotypes derived from these 11 identified SNPs were significantly associated with MFD (p < 0.05). In conclusion, our results suggest that DKK1 may be a major gene controlling Wool Production and quality traits, also the identified SNPs (SNPs5, 8, 9 and 10) might be used as potential molecular markers for improving sheep Wool Production and quality in sheep breeding.
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association of methionine synthase gene polymorphisms with Wool Production and quality traits in chinese merino population
Journal of Animal Science, 2015Co-Authors: En-guang Rong, Zhipeng Wang, Huanming Yang, Zhiwei Zhang, Xiaohong Yan, Ning WangAbstract:Methionine synthase (MTR) plays a crucial role in maintaining homeostasis of intracellular methionine, folate, and homocysteine, and its activity correlates with DNA methylation in many mammalian tissues. Our previous genomewide association study identified that 1 SNP located in the gene was associated with several Wool Production and quality traits in Chinese Merino. To confirm the potential involvement of the gene in sheep Wool Production and quality traits, we performed sheep tissue expression profiling, SNP detection, and association analysis with sheep Wool Production and quality traits. The semiquantitative reverse transcription PCR analysis showed that the gene was differentially expressed in skin from Merino and Kazak sheep. The sequencing analysis identified a total of 13 SNP in the gene from Chinese Merino sheep. Comparison of the allele frequencies revealed that these 13 identified SNP were significantly different among the 6 tested Chinese Merino strains ( < 0.001). Linkage disequilibrium analysis showed that SNP 3 to 11 were strongly linked in a single haplotype block in the tested population. Association analysis showed that SNP 2 to 11 were significantly associated with the average Wool fiber diameter and the fineness SD and that SNP 4 to 11 were significantly associated with the CV of fiber diameter trait ( < 0.05). Single nucleotide polymorphism 2 and SNP 5 to 12 were weakly associated with Wool crimp. Similarly, the haplotypes derived from these 13 identified SNP were also significantly associated with the average Wool fiber diameter, fineness SD, and the CV of fiber diameter ( < 0.05). Our results suggest that is a candidate gene for sheep Wool Production and quality traits, and the identified SNP might be used in sheep breeding.
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genome wide association study for Wool Production traits in a chinese merino sheep population
PLOS ONE, 2014Co-Authors: Zhipeng Wang, Hua Yang, En-guang Rong, Shouzhi Wang, Hui Zhang, Wenyu Pei, Ning WangAbstract:Genome-wide association studies (GWAS) provide a powerful approach for identifying quantitative trait loci without prior knowledge of location or function. To identify loci associated with Wool Production traits, we performed a genome-wide association study on a total of 765 Chinese Merino sheep (JunKen type) genotyped with 50 K single nucleotide polymorphisms (SNPs). In the present study, five Wool Production traits were examined: fiber diameter, fiber diameter coefficient of variation, fineness dispersion, staple length and crimp. We detected 28 genome-wide significant SNPs for fiber diameter, fiber diameter coefficient of variation, fineness dispersion, and crimp trait in the Chinese Merino sheep. About 43% of the significant SNP markers were located within known or predicted genes, including YWHAZ, KRTCAP3, TSPEAR, PIK3R4, KIF16B, PTPN3, GPRC5A, DDX47, TCF9, TPTE2, EPHA5 and NBEA genes. Our results not only confirm the results of previous reports, but also provide a suite of novel SNP markers and candidate genes associated with Wool traits. Our findings will be useful for exploring the genetic control of Wool traits in sheep.
Abdulkerim Yörükoğlu - One of the best experts on this subject based on the ideXlab platform.
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Investigation of boron usability in rock Wool Production
Construction and Building Materials, 2020Co-Authors: Abdulkerim Yörükoğlu, Fatih Akkurt, Serdar ÇulhaAbstract:Abstract The effect of boron addition on basalt and basalt-dolomite mixtures was tested for rock Wool Production. The melting temperatures and the viscosities of the melts were determined and SEM and EDS analysis on some samples were done to find out the effect of boron addition. It was observed that boron addition lowered the melting temperatures noticeably, slight increases in the viscosities were at acceptable levels and no negative structural changes were caused. It was concluded that boron could be added for the rock Wool Production and the quantity to be used could be adjusted considering the melting temperature and the viscosity value.
