The Experts below are selected from a list of 969 Experts worldwide ranked by ideXlab platform
A Jurjus - One of the best experts on this subject based on the ideXlab platform.
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modulation of Wound Contracture α smooth muscle actin and multispecific vitronectin receptor integrin αvβ3 in the rabbit s experimental model
International Wound Journal, 2009Co-Authors: Cynthia El G Kahi, Bishara S Atiyeh, Inaya Hajj Hussein, Rosalyne Jurjus, Saad A Dibo, A JurjusAbstract:The myofibroblast, a major component of granulation tissue, is a key cell during Wound healing, tissue repair and connective tissue remodelling. Persistence of myofibroblasts within a fibrotic lesion leads to excessive scarring impairing function and aesthetics. Various Wound-healing cytokines can be modulated by topical application of active agents to promote optimal Wound healing and improve scar quality. Thus, the myofibroblast may represent an important target for Wound-healing modulation to improve the evolution of conditions such as hypertrophic scars. The purpose of this work is to study the modulation of myofibroblasts and integrin αvβ3 in a full thickness Wound performed on rabbits treated with different topical agents using: (1) saline, (2) Tegaderm occlusive dressing (3) silver sulfadiazine and (4) moist exposed burn ointment (MEBO). The reepithelialisation was 4 days faster in the MEBO group compared with the other therapies with less oedema formation, delayed contraction, less inflammatory cells and the lowest transepidermal water loss (TEWL) resulting in a soft scar. Although α-smooth muscle actin (α-SMA) was the highest around day 12 in the MEBO group, Wound contraction and myofibroblast’s activity were the least for the same period probably because of a downregulation of the integrin αvβ3. It seems that the effect of MEBO could be more pronounced on force transmission rather then on force generation. Greater insight into the pathology of scars may translate into non surgical treatments in the future and further work in myofibroblast biology will eventually result in efficient pharmacological tools, improving the evolution of healing and scar formation.
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modulation of Wound Contracture α smooth muscle actin and multispecific vitronectin receptor integrin αvβ3 in the rabbit s experimental model
International Wound Journal, 2009Co-Authors: Cynthia El G Kahi, Bishara S Atiyeh, Inaya Hajj Hussein, Rosalyne Jurjus, Saad A Dibo, A JurjusAbstract:The myofibroblast, a major component of granulation tissue, is a key cell during Wound healing, tissue repair and connective tissue remodelling. Persistence of myofibroblasts within a fibrotic lesion leads to excessive scarring impairing function and aesthetics. Various Wound-healing cytokines can be modulated by topical application of active agents to promote optimal Wound healing and improve scar quality. Thus, the myofibroblast may represent an important target for Wound-healing modulation to improve the evolution of conditions such as hypertrophic scars. The purpose of this work is to study the modulation of myofibroblasts and integrin alphavbeta3 in a full thickness Wound performed on rabbits treated with different topical agents using: (1) saline, (2) Tegaderm occlusive dressing (3) silver sulfadiazine and (4) moist exposed burn ointment (MEBO). The reepithelialisation was 4 days faster in the MEBO group compared with the other therapies with less oedema formation, delayed contraction, less inflammatory cells and the lowest transepidermal water loss (TEWL) resulting in a soft scar. Although alpha-smooth muscle actin (alpha-SMA) was the highest around day 12 in the MEBO group, Wound contraction and myofibroblast's activity were the least for the same period probably because of a downregulation of the integrin alphavbeta3. It seems that the effect of MEBO could be more pronounced on force transmission rather then on force generation. Greater insight into the pathology of scars may translate into non surgical treatments in the future and further work in myofibroblast biology will eventually result in efficient pharmacological tools, improving the evolution of healing and scar formation.
Mohammad Mahdi Zangeneh - One of the best experts on this subject based on the ideXlab platform.
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pharmacological histopathological and biochemical assessments of cutaneous Wound healing potential of tragopogon graminifolius aqueous extract ointment in rats
Comparative Haematology International, 2019Co-Authors: Samaneh Goorani, Niloofar Seydi, Akram Zangeneh, Hassan Morovvati, Mohammad Mahdi ZangenehAbstract:The recent experiments have revealed the property of ethno-medicinal plants on the treatment of the cutaneous Wound. The purpose of the experiment was to investigate the Wound healing potential of Tragopogon graminifolius aqueous extract ointment. DPPH free radical scavenging test was carried out to examine the antioxidant effect of T. graminifolius aqueous extract, which indicated similar antioxidant activity with butylated hydroxy toluene (BHT) as a positive control. In vivo design, 120 Sprague Dawley male rats were used. After creating the cutaneous Wound, the animals were randomly divided into four groups; untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% T. graminifolius aqueous extract ointment (3 g of T. graminifolius aqueous extract + 97 g base ointment). On days 10, 20, and 30 after creating the Wound, for histopathological and biochemical analysis of the cutaneous Wound healing trend, a section was prepared from all dermal thicknesses. The data were analyzed using one-way ANOVA followed by Duncan post hoc test. On days 10, 20, and 30, T. graminifolius aqueous extract ointment could significantly (p ≤ 0.05) decrease the level of the Wound area, total cell, macrophage, lymphocyte, and neutrophil, and enhance the level of Wound Contracture, fibrocyte, hexuronic acid, hexosamine, and hydroxyproline as compared to the basal ointment and control groups. In conclusion, the acquired findings showed the cutaneous Wound healing potential of T. graminifolius aqueous extract ointment.
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in vitro and in vivo evaluation of cytotoxicity antioxidant antibacterial antifungal and cutaneous Wound healing properties of gold nanoparticles produced via a green chemistry synthesis using gundelia tournefortii l as a capping and reducing agent
Applied Organometallic Chemistry, 2019Co-Authors: Mohsen Zhaleh, Mohammad Mahdi Zangeneh, Akram Zangeneh, Niloofar Seydi, Samaneh Goorani, Reza Tahvilian, Elham PirabbasiAbstract:The exploitation of various plant materials for the biosynthesis of nanoparticles is considered a green technology because it does not involve any harmful chemicals. The aim of the experiment was chemical characterization and evaluation of cytotoxicity, antioxidant, antibacterial, antifungal, and cutaneous Wound healing activities of gold nanoparticles using aqueous extract of Gundelia tournefortii L. leaves (AuNPs@GT). These nanoparticles were characterized by fourier transformed infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDS), and UV-visible spectroscopy. DPPH free radical scavenging test was done to assess the antioxidant properties, which indicated similar antioxidant potentials for AuNPs@GT and butylated hydroxytoluene. Agar diffusion tests were applied to determine the antibacterial and antifungal characteristics. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) were specified by macro-broth dilution assay. AuNPs@GT indicated higher antibacterial and antifungal effects than all standard antibiotics (p <= 0.01). Also, AuNPs@GT inhibited the growth of all bacteria and fungi and removed them at 2-4 mg/mL concentrations (p <= 0.01). In vivo experiment, after creating the cutaneous Wound, the rats were randomly divided into six groups: untreated control, treatment with Eucerin basal ointment, treatment with 3 tetracycline ointment, treatment with 0.2 HAuCl4 ointment, treatment with 0.2 G. tournefortii ointment, and treatment with 0.2 AuNPs@GT ointment. These groups were treated for 10 days. For histopathological and biochemical analysis of the healing trend, a 3 x 3 cm section was prepared from all dermal thicknesses at day 10. Use of AuNPs@GT ointment in the treatment groups substantially reduced (p <= 0.01) the Wound area, total cells, neutrophil, and lymphocyte and remarkably raised (p <= 0.01) the Wound Contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, fibroblast, and fibrocytes/fibroblast rate compared to other groups. The synthesized AuNPs@GT had great cell viability dose-dependently (Investigating the effect of the plant on HUVEC cell line) and revealed this method was nontoxic. The results showed that the leave aqueous extract of G. tournefortii is very good bioreductant in the synthesis of gold nanoparticles for treatment of bacterial, fungal, and skin diseases.
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ziziphora clinopodioides lam leaves aqueous extract mediated synthesis of zinc nanoparticles and their antibacterial antifungal cytotoxicity antioxidant and cutaneous Wound healing properties under in vitro and in vivo conditions
Applied Organometallic Chemistry, 2019Co-Authors: Behnam Mahdavi, Mohammad Mahdi Zangeneh, Akram Zangeneh, Sania Saneei, Maryam Qorbani, Mohsen Zhaleh, Elham Pirabbasi, Naser Abbasi, Hori GhaneialvarAbstract:LamZiziphora clinopodioides Biosynthesis of metal nanoparticles using plant medicine is under exploration is due to wide biomedica applications and research interest in nanotechnology, the recent study was assessing green synthesis of zinc nanoparticle using ), the use of plant material Ziziphoraleaves extract (ZnNPs@ not only makes the process eco-friendly but also the abundance makes it more economical. Also, in this study, Vis. and- were characterized using different techniques including UVZiziphora were synthesized in aqueous medium using the plant extract as stabilizing and reducing agents. The synthesized ZnNPs@Ziziphora conditions. ZnNPs@in vivo and in vitro under Ziziphorawe investigated the therapeutical properties of ZnNPs@ FT-IR spectroscopy, . SEM images exhibited a uniform spherical morphology in size of 32.34 Thermal Gravimetric Analysis (TGA)ray diffraction (XRD), scanning electron microscopy (SEM), and Energy Dispersive X-ray Spectrometry (EDS), and-X Escherichia coli, and Pseudomonas aeruginosa, Salmonella typhimurium part of this study, these nanoparticles indicated excellent antibacterial properties against Gram-negative bacteria (in vitronm for the biosynthesized nanoparticles. In the biological , andCandida krusei, Candida albicans, Candida glabrata), antifungal potentials against Bacillus subtilis, andStaphylococcus aureus, Streptococcus pneumoniaO157:H7) and Gram-positive bacteria ( leaves aqueous extract can be used to yield zinc nanoparticles with a significant amount of antibacterial, antifungal, antioxidant, and cutaneous Wound healing properties without any cytotoxicity.Z. clinopodioides ointment ameliorated the cutaneous Wounds with increasing the levels of Wound Contracture, vessel, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and decreeing the Wound area, total cells, and lymphocyte compared to other groups in rats. The results of UV, FT-IR, XRD, FE-SEM, EDS, and TGA confirm that the Ziziphora part of our experiment, ZnNPs@in vivo, non-cytotoxicity effect against human umbilical vein endothelial cells, and antioxidant activity against DPPH. In the biological Candida guilliermondii
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pharmacological biochemical and histopathological evaluations of cutaneous Wound healing property of mentha piperita aqueous extract ointment
Comparative Haematology International, 2019Co-Authors: Mohammad Mahdi Zangeneh, Akram Zangeneh, Rohallah Moradi, Niloofar SeydiAbstract:In our study, we evaluated cutaneous Wound healing potential of Mentha piperita aqueous extract ointment in rats. In vivo design, after creating the cutaneous Wound on the back of the rats, the animals were randomly divided into four groups: untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% M. piperita aqueous extract ointment. The groups were treated for 30 days. For biochemical and histopathological analysis of the cutaneous Wound healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses on days 10, 20, and 30. The data were analyzed by SPSS-21 software. The use of M. piperita aqueous extract ointment in the treatment groups led to remarkable reduction (p < 0.05) in the levels of Wound area, total cells, neutrophil, lymphocyte, and macrophage and remarkable increase (p < 0.05) in the levels of Wound Contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and the rate of fibrocyte to fibroblast compared with the control and basal ointment groups. According to the findings, M. piperita aqueous extract ointment can cure the cutaneous Wound.
Bishara S Atiyeh - One of the best experts on this subject based on the ideXlab platform.
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modulation of Wound Contracture α smooth muscle actin and multispecific vitronectin receptor integrin αvβ3 in the rabbit s experimental model
International Wound Journal, 2009Co-Authors: Cynthia El G Kahi, Bishara S Atiyeh, Inaya Hajj Hussein, Rosalyne Jurjus, Saad A Dibo, A JurjusAbstract:The myofibroblast, a major component of granulation tissue, is a key cell during Wound healing, tissue repair and connective tissue remodelling. Persistence of myofibroblasts within a fibrotic lesion leads to excessive scarring impairing function and aesthetics. Various Wound-healing cytokines can be modulated by topical application of active agents to promote optimal Wound healing and improve scar quality. Thus, the myofibroblast may represent an important target for Wound-healing modulation to improve the evolution of conditions such as hypertrophic scars. The purpose of this work is to study the modulation of myofibroblasts and integrin αvβ3 in a full thickness Wound performed on rabbits treated with different topical agents using: (1) saline, (2) Tegaderm occlusive dressing (3) silver sulfadiazine and (4) moist exposed burn ointment (MEBO). The reepithelialisation was 4 days faster in the MEBO group compared with the other therapies with less oedema formation, delayed contraction, less inflammatory cells and the lowest transepidermal water loss (TEWL) resulting in a soft scar. Although α-smooth muscle actin (α-SMA) was the highest around day 12 in the MEBO group, Wound contraction and myofibroblast’s activity were the least for the same period probably because of a downregulation of the integrin αvβ3. It seems that the effect of MEBO could be more pronounced on force transmission rather then on force generation. Greater insight into the pathology of scars may translate into non surgical treatments in the future and further work in myofibroblast biology will eventually result in efficient pharmacological tools, improving the evolution of healing and scar formation.
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modulation of Wound Contracture α smooth muscle actin and multispecific vitronectin receptor integrin αvβ3 in the rabbit s experimental model
International Wound Journal, 2009Co-Authors: Cynthia El G Kahi, Bishara S Atiyeh, Inaya Hajj Hussein, Rosalyne Jurjus, Saad A Dibo, A JurjusAbstract:The myofibroblast, a major component of granulation tissue, is a key cell during Wound healing, tissue repair and connective tissue remodelling. Persistence of myofibroblasts within a fibrotic lesion leads to excessive scarring impairing function and aesthetics. Various Wound-healing cytokines can be modulated by topical application of active agents to promote optimal Wound healing and improve scar quality. Thus, the myofibroblast may represent an important target for Wound-healing modulation to improve the evolution of conditions such as hypertrophic scars. The purpose of this work is to study the modulation of myofibroblasts and integrin alphavbeta3 in a full thickness Wound performed on rabbits treated with different topical agents using: (1) saline, (2) Tegaderm occlusive dressing (3) silver sulfadiazine and (4) moist exposed burn ointment (MEBO). The reepithelialisation was 4 days faster in the MEBO group compared with the other therapies with less oedema formation, delayed contraction, less inflammatory cells and the lowest transepidermal water loss (TEWL) resulting in a soft scar. Although alpha-smooth muscle actin (alpha-SMA) was the highest around day 12 in the MEBO group, Wound contraction and myofibroblast's activity were the least for the same period probably because of a downregulation of the integrin alphavbeta3. It seems that the effect of MEBO could be more pronounced on force transmission rather then on force generation. Greater insight into the pathology of scars may translate into non surgical treatments in the future and further work in myofibroblast biology will eventually result in efficient pharmacological tools, improving the evolution of healing and scar formation.
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scar quality and physiologic barrier function restoration after moist and moist exposed dressings of partial thickness Wounds
Dermatologic Surgery, 2003Co-Authors: Bishara S Atiyeh, Kusai A Elmusa, Ruwayda DhamAbstract:BACKGROUND. There is growing evidence of improved healing of full- and partial-thickness cutaneous Wounds in wet and moist environments. Retention of biologic fluids over the Wound prevents desiccation of denuded dermis or deeper tissues and allows faster and unimpeded migration of keratinocytes over the Wound surface. It allows also the naturally occurring cytokines and growth factors to exert their beneficial effect on Wound Contracture and re-epithelialization. Despite all of these documented benefits, applying the moist healing principles to large surface areas, in particular to large burns, is hindered by the major technical handicap of creating and maintaining a sealed moist environment over these areas. METHODS. From January to September 2001, healing of partial-thickness skin graft donor sites was studied in a prospective comparative study of two types of moist dressings, Tegaderm (3M Health Care, St. Paul, MN), a semipermeable membrane occlusive dressing, and moist exposed burn ointment (MEBO) (Julphar; Gulf Pharmaceutical Industries, United Arab of Emirates), an ointment that can provide a moist environment without the need of an overlying occlusive dressing. Healing was assessed both clinically and with serial measurements of transepidermal water loss (TEWL) and moisture. Following healing, scar quality was evaluated by two members of the team separately using a visual analog scale. Results were statistically analyzed. RESULTS. Faster healing was observed clinically with MEBO application. Physiologic healing as determined by TEWL measurements occurred at an extremely significant earlier stage for MEBO, and this was associated with better scar quality, demonstrating a positive relationship between function and cosmetic appearance. Moreover, the ointment is definitely easier to apply than the occlusive self-adhesive membrane, which requires some degree of dexterity and expertise. CONCLUSION. MEBO application is an effective and valid alternative to conventional occlusive dressings. Moreover, the observed improved anatomic and physiologic healing indicates that MEBO may have a positive effect on healing more that the mere fact of passive moisture retention. B.S. ATIYEH, MD, FACS, K.A. EL-MUSA, AND R. DHAM, PhD HAVE INDICATED NO SIGNIFICANT INTEREST WITH COMMERCIAL SUPPORTERS.
Cynthia El G Kahi - One of the best experts on this subject based on the ideXlab platform.
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modulation of Wound Contracture α smooth muscle actin and multispecific vitronectin receptor integrin αvβ3 in the rabbit s experimental model
International Wound Journal, 2009Co-Authors: Cynthia El G Kahi, Bishara S Atiyeh, Inaya Hajj Hussein, Rosalyne Jurjus, Saad A Dibo, A JurjusAbstract:The myofibroblast, a major component of granulation tissue, is a key cell during Wound healing, tissue repair and connective tissue remodelling. Persistence of myofibroblasts within a fibrotic lesion leads to excessive scarring impairing function and aesthetics. Various Wound-healing cytokines can be modulated by topical application of active agents to promote optimal Wound healing and improve scar quality. Thus, the myofibroblast may represent an important target for Wound-healing modulation to improve the evolution of conditions such as hypertrophic scars. The purpose of this work is to study the modulation of myofibroblasts and integrin αvβ3 in a full thickness Wound performed on rabbits treated with different topical agents using: (1) saline, (2) Tegaderm occlusive dressing (3) silver sulfadiazine and (4) moist exposed burn ointment (MEBO). The reepithelialisation was 4 days faster in the MEBO group compared with the other therapies with less oedema formation, delayed contraction, less inflammatory cells and the lowest transepidermal water loss (TEWL) resulting in a soft scar. Although α-smooth muscle actin (α-SMA) was the highest around day 12 in the MEBO group, Wound contraction and myofibroblast’s activity were the least for the same period probably because of a downregulation of the integrin αvβ3. It seems that the effect of MEBO could be more pronounced on force transmission rather then on force generation. Greater insight into the pathology of scars may translate into non surgical treatments in the future and further work in myofibroblast biology will eventually result in efficient pharmacological tools, improving the evolution of healing and scar formation.
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modulation of Wound Contracture α smooth muscle actin and multispecific vitronectin receptor integrin αvβ3 in the rabbit s experimental model
International Wound Journal, 2009Co-Authors: Cynthia El G Kahi, Bishara S Atiyeh, Inaya Hajj Hussein, Rosalyne Jurjus, Saad A Dibo, A JurjusAbstract:The myofibroblast, a major component of granulation tissue, is a key cell during Wound healing, tissue repair and connective tissue remodelling. Persistence of myofibroblasts within a fibrotic lesion leads to excessive scarring impairing function and aesthetics. Various Wound-healing cytokines can be modulated by topical application of active agents to promote optimal Wound healing and improve scar quality. Thus, the myofibroblast may represent an important target for Wound-healing modulation to improve the evolution of conditions such as hypertrophic scars. The purpose of this work is to study the modulation of myofibroblasts and integrin alphavbeta3 in a full thickness Wound performed on rabbits treated with different topical agents using: (1) saline, (2) Tegaderm occlusive dressing (3) silver sulfadiazine and (4) moist exposed burn ointment (MEBO). The reepithelialisation was 4 days faster in the MEBO group compared with the other therapies with less oedema formation, delayed contraction, less inflammatory cells and the lowest transepidermal water loss (TEWL) resulting in a soft scar. Although alpha-smooth muscle actin (alpha-SMA) was the highest around day 12 in the MEBO group, Wound contraction and myofibroblast's activity were the least for the same period probably because of a downregulation of the integrin alphavbeta3. It seems that the effect of MEBO could be more pronounced on force transmission rather then on force generation. Greater insight into the pathology of scars may translate into non surgical treatments in the future and further work in myofibroblast biology will eventually result in efficient pharmacological tools, improving the evolution of healing and scar formation.
Akram Zangeneh - One of the best experts on this subject based on the ideXlab platform.
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pharmacological histopathological and biochemical assessments of cutaneous Wound healing potential of tragopogon graminifolius aqueous extract ointment in rats
Comparative Haematology International, 2019Co-Authors: Samaneh Goorani, Niloofar Seydi, Akram Zangeneh, Hassan Morovvati, Mohammad Mahdi ZangenehAbstract:The recent experiments have revealed the property of ethno-medicinal plants on the treatment of the cutaneous Wound. The purpose of the experiment was to investigate the Wound healing potential of Tragopogon graminifolius aqueous extract ointment. DPPH free radical scavenging test was carried out to examine the antioxidant effect of T. graminifolius aqueous extract, which indicated similar antioxidant activity with butylated hydroxy toluene (BHT) as a positive control. In vivo design, 120 Sprague Dawley male rats were used. After creating the cutaneous Wound, the animals were randomly divided into four groups; untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% T. graminifolius aqueous extract ointment (3 g of T. graminifolius aqueous extract + 97 g base ointment). On days 10, 20, and 30 after creating the Wound, for histopathological and biochemical analysis of the cutaneous Wound healing trend, a section was prepared from all dermal thicknesses. The data were analyzed using one-way ANOVA followed by Duncan post hoc test. On days 10, 20, and 30, T. graminifolius aqueous extract ointment could significantly (p ≤ 0.05) decrease the level of the Wound area, total cell, macrophage, lymphocyte, and neutrophil, and enhance the level of Wound Contracture, fibrocyte, hexuronic acid, hexosamine, and hydroxyproline as compared to the basal ointment and control groups. In conclusion, the acquired findings showed the cutaneous Wound healing potential of T. graminifolius aqueous extract ointment.
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in vitro and in vivo evaluation of cytotoxicity antioxidant antibacterial antifungal and cutaneous Wound healing properties of gold nanoparticles produced via a green chemistry synthesis using gundelia tournefortii l as a capping and reducing agent
Applied Organometallic Chemistry, 2019Co-Authors: Mohsen Zhaleh, Mohammad Mahdi Zangeneh, Akram Zangeneh, Niloofar Seydi, Samaneh Goorani, Reza Tahvilian, Elham PirabbasiAbstract:The exploitation of various plant materials for the biosynthesis of nanoparticles is considered a green technology because it does not involve any harmful chemicals. The aim of the experiment was chemical characterization and evaluation of cytotoxicity, antioxidant, antibacterial, antifungal, and cutaneous Wound healing activities of gold nanoparticles using aqueous extract of Gundelia tournefortii L. leaves (AuNPs@GT). These nanoparticles were characterized by fourier transformed infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDS), and UV-visible spectroscopy. DPPH free radical scavenging test was done to assess the antioxidant properties, which indicated similar antioxidant potentials for AuNPs@GT and butylated hydroxytoluene. Agar diffusion tests were applied to determine the antibacterial and antifungal characteristics. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) were specified by macro-broth dilution assay. AuNPs@GT indicated higher antibacterial and antifungal effects than all standard antibiotics (p <= 0.01). Also, AuNPs@GT inhibited the growth of all bacteria and fungi and removed them at 2-4 mg/mL concentrations (p <= 0.01). In vivo experiment, after creating the cutaneous Wound, the rats were randomly divided into six groups: untreated control, treatment with Eucerin basal ointment, treatment with 3 tetracycline ointment, treatment with 0.2 HAuCl4 ointment, treatment with 0.2 G. tournefortii ointment, and treatment with 0.2 AuNPs@GT ointment. These groups were treated for 10 days. For histopathological and biochemical analysis of the healing trend, a 3 x 3 cm section was prepared from all dermal thicknesses at day 10. Use of AuNPs@GT ointment in the treatment groups substantially reduced (p <= 0.01) the Wound area, total cells, neutrophil, and lymphocyte and remarkably raised (p <= 0.01) the Wound Contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, fibroblast, and fibrocytes/fibroblast rate compared to other groups. The synthesized AuNPs@GT had great cell viability dose-dependently (Investigating the effect of the plant on HUVEC cell line) and revealed this method was nontoxic. The results showed that the leave aqueous extract of G. tournefortii is very good bioreductant in the synthesis of gold nanoparticles for treatment of bacterial, fungal, and skin diseases.
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ziziphora clinopodioides lam leaves aqueous extract mediated synthesis of zinc nanoparticles and their antibacterial antifungal cytotoxicity antioxidant and cutaneous Wound healing properties under in vitro and in vivo conditions
Applied Organometallic Chemistry, 2019Co-Authors: Behnam Mahdavi, Mohammad Mahdi Zangeneh, Akram Zangeneh, Sania Saneei, Maryam Qorbani, Mohsen Zhaleh, Elham Pirabbasi, Naser Abbasi, Hori GhaneialvarAbstract:LamZiziphora clinopodioides Biosynthesis of metal nanoparticles using plant medicine is under exploration is due to wide biomedica applications and research interest in nanotechnology, the recent study was assessing green synthesis of zinc nanoparticle using ), the use of plant material Ziziphoraleaves extract (ZnNPs@ not only makes the process eco-friendly but also the abundance makes it more economical. Also, in this study, Vis. and- were characterized using different techniques including UVZiziphora were synthesized in aqueous medium using the plant extract as stabilizing and reducing agents. The synthesized ZnNPs@Ziziphora conditions. ZnNPs@in vivo and in vitro under Ziziphorawe investigated the therapeutical properties of ZnNPs@ FT-IR spectroscopy, . SEM images exhibited a uniform spherical morphology in size of 32.34 Thermal Gravimetric Analysis (TGA)ray diffraction (XRD), scanning electron microscopy (SEM), and Energy Dispersive X-ray Spectrometry (EDS), and-X Escherichia coli, and Pseudomonas aeruginosa, Salmonella typhimurium part of this study, these nanoparticles indicated excellent antibacterial properties against Gram-negative bacteria (in vitronm for the biosynthesized nanoparticles. In the biological , andCandida krusei, Candida albicans, Candida glabrata), antifungal potentials against Bacillus subtilis, andStaphylococcus aureus, Streptococcus pneumoniaO157:H7) and Gram-positive bacteria ( leaves aqueous extract can be used to yield zinc nanoparticles with a significant amount of antibacterial, antifungal, antioxidant, and cutaneous Wound healing properties without any cytotoxicity.Z. clinopodioides ointment ameliorated the cutaneous Wounds with increasing the levels of Wound Contracture, vessel, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and decreeing the Wound area, total cells, and lymphocyte compared to other groups in rats. The results of UV, FT-IR, XRD, FE-SEM, EDS, and TGA confirm that the Ziziphora part of our experiment, ZnNPs@in vivo, non-cytotoxicity effect against human umbilical vein endothelial cells, and antioxidant activity against DPPH. In the biological Candida guilliermondii
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pharmacological biochemical and histopathological evaluations of cutaneous Wound healing property of mentha piperita aqueous extract ointment
Comparative Haematology International, 2019Co-Authors: Mohammad Mahdi Zangeneh, Akram Zangeneh, Rohallah Moradi, Niloofar SeydiAbstract:In our study, we evaluated cutaneous Wound healing potential of Mentha piperita aqueous extract ointment in rats. In vivo design, after creating the cutaneous Wound on the back of the rats, the animals were randomly divided into four groups: untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% M. piperita aqueous extract ointment. The groups were treated for 30 days. For biochemical and histopathological analysis of the cutaneous Wound healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses on days 10, 20, and 30. The data were analyzed by SPSS-21 software. The use of M. piperita aqueous extract ointment in the treatment groups led to remarkable reduction (p < 0.05) in the levels of Wound area, total cells, neutrophil, lymphocyte, and macrophage and remarkable increase (p < 0.05) in the levels of Wound Contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and the rate of fibrocyte to fibroblast compared with the control and basal ointment groups. According to the findings, M. piperita aqueous extract ointment can cure the cutaneous Wound.
