The Experts below are selected from a list of 5538 Experts worldwide ranked by ideXlab platform
Dominique Lison - One of the best experts on this subject based on the ideXlab platform.
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Mind your Assays: Misleading cytotoxicity with the WST-1 Assay in the presence of manganese.
PloS one, 2020Co-Authors: Eleonora Scarcello, Alexia Lambremont, Rita Vanbever, Pascal Jacques, Dominique LisonAbstract:The WST-1 Assay is the most common test to assess the in vitro cytotoxicity of chemicals. Tetrazolium-based Assays can, however, be affected by the interference of tested chemicals, including carbon nanotubes or Mg particles. Here, we report a new interference of Mn materials with the WST-1 Assay. Endothelial cells exposed to Mn particles (Mn alone or Fe-Mn alloy from 50 to 1600 μg/ml) were severely damaged according to the WST-1 Assay, but not the ATP content Assay. Subsequent experiments revealed that Mn particles interfere with the reduction of the tetrazolium salt to formazan. Therefore, the WST-1 Assay is not suitable to evaluate the in vitro cytotoxicity of Mn-containing materials, and luminescence-based Assays such as CellTiter-Glo® appear more appropriate.
Zhang Quan-xin - One of the best experts on this subject based on the ideXlab platform.
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A Simple and Rapid Method of WST-1 Assay for Evaluation of Antitumor Activity
Journal of Tropical Medicine, 2005Co-Authors: Zhang Quan-xinAbstract:Objective To establish a rapid and sensitive method to evaluate the antitumor activity of curcumin and indole-3-carbinol. Methods CNE1 cells were used for the detection of antitumor activity. CNE1 cells were incubated with CU (at the dose of 1.7×10-3, 8.5×10-3, 42.5×10-3 g/ml),or I3C (at the dose of 3.0×10-3, 15.0×10-3, 45.0×10-3g/ml) at 37 ℃ for 48 h. After incubation, WST-1 reagent was added to the well and measured the OD at 570nm for the determination of anti-tumor activity. In parallel, the anti-tumor activity was also detected with the method described in GB/T16175-1996, and the OD values were recorded at 588nm. Results The results detected with WST-1 Assay were correlated with the results of classic arbitration method stipulated by national criterion. Moreover, WST-1 Assay was more simple, rapid, efficient, acute and objective. Conclusion The WST-1 Assay might be used as an Assay to screen and evaluate antitumor substance.
Eleonora Scarcello - One of the best experts on this subject based on the ideXlab platform.
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Mind your Assays: Misleading cytotoxicity with the WST-1 Assay in the presence of manganese.
PloS one, 2020Co-Authors: Eleonora Scarcello, Alexia Lambremont, Rita Vanbever, Pascal Jacques, Dominique LisonAbstract:The WST-1 Assay is the most common test to assess the in vitro cytotoxicity of chemicals. Tetrazolium-based Assays can, however, be affected by the interference of tested chemicals, including carbon nanotubes or Mg particles. Here, we report a new interference of Mn materials with the WST-1 Assay. Endothelial cells exposed to Mn particles (Mn alone or Fe-Mn alloy from 50 to 1600 μg/ml) were severely damaged according to the WST-1 Assay, but not the ATP content Assay. Subsequent experiments revealed that Mn particles interfere with the reduction of the tetrazolium salt to formazan. Therefore, the WST-1 Assay is not suitable to evaluate the in vitro cytotoxicity of Mn-containing materials, and luminescence-based Assays such as CellTiter-Glo® appear more appropriate.
Xu Changsheng - One of the best experts on this subject based on the ideXlab platform.
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Fluvastatin upregulates L-type CA2+ channel α1C expression and induces cell arrest in vascular smooth muscle cells
Heart, 2011Co-Authors: Han Ying, Xie Liangdi, Ou-yang Qiu-fang, Xu ChangshengAbstract:Objective To investigate the effect of fluvastatin on L-type calcium channel (LTCC) in the cultured aortic vascular smooth muscle cells (VSMCs) and its alteration associated with proliferation. Method The proliferative phase of VSMCs was evaluated by water-soluble tetrazolium (WST-1) Assay. LTCC-α1C mRNA and protein were determined by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Cell cycle, the ratio of G0 +G1 phase cells and S phase cells were analysed using flow cytometry analysis after VSMCs were exposed to fluvastatin. Result Fluvastatin showed an inhibitory effect on VSMCs growth in a time-dependent manner as assessed by WST-1 Assay, proliferation of VSMCs was suppressed by 2.1-fold after the administration of fluvastatin for 24 h. The down-regulation of LTCC-α1C subunit induced by platelet derived growth factor (PDGF)-BB was significantly restored by the treatment of fluvastatin. The mRNA and protein levels of LTCC-α1C subunit of cultured VSMCs were significantly decreased after incubation with PDGF (10 µg/l) (p −5 mol/l) induced a 4.1-fold increase in mRNA level and a 1.2-fold increase in protein expression of LTCC-α1C subunit. The ratio of G0 +G1 phase cells increased (p Conclusion Fluvastatin may induce cell growth arrest at G0, G1 phase of cell cycle and upregulate LTCC-α1C subunit expression in cultured VSMCs.
Makoto Araie - One of the best experts on this subject based on the ideXlab platform.
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Toxicity evaluation of antiglaucoma drugs using stratified human cultivated corneal epithelial sheets.
Investigative ophthalmology & visual science, 2012Co-Authors: Suguru Nakagawa, Mikiko Kimakura, Tomohiko Usui, Seiichi Yokoo, Shiro Amano, Sachiko Omichi, Yosai Mori, Kazunori Miyata, Makoto Aihara, Makoto AraieAbstract:PURPOSE. To investigate the toxicity profiles of seven antiglaucoma topical eye drops and benzalkonium chloride (BAC) using stratified cultivated human corneal epithelial cell sheets (HCES) in a serum-free culture system. METHODS. A range of prostaglandin analogies and preservatives, including BAC, sofZia (SZ), sodium benzoate (SB), and polyquaternium-1 (PQ) were tested. The barrier function and cell viability were examined by a carboxyfluorescein permeability Assay and WST-1 Assay. Histological evaluation of the HCES was also performed after application of each solution. RESULTS. The carboxyfluorescein permeability Assay had a higher sensitivity for the detection of toxicity of test solutions than the WST-1 Assay or histological examination. Latanoprost BAC, latanoprost/timolol BAC, and 0.02% or higher concentration of BAC were the most toxic, followed by latanoprost SB, latanoprost preservative-free, BAC 0.002%, and travoprost/ latanoprost PQ. Travoprost SZ and tafluprost BAC (preserved with 0.001% BAC) was the least toxic in our experimental conditions. CONCLUSIONS. The carboxyfluorescein permeability Assay using HCES in a serum-free system was the most useful for the quantification of toxicity of ophthalmic solutions. Among the regimens examined, a BAC concentration of 0.001% or lower or non-BAC preservative sofZia was suggested to be the least toxic to the ocular surface. (Invest Ophthalmol Vis Sci. 2012; 53:5154‐5160) DOI:10.1167/iovs.12-9685
