The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Amir Tavakkol - One of the best experts on this subject based on the ideXlab platform.
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Storage phosphor imaging technique improves the accuracy of RNA quantitation using 32P-labeled cDNA probes.
BioTechniques, 1994Co-Authors: Christos C. Zouboulis, Amir TavakkolAbstract:The use of the storage phosphor imaging technique to quantitate radioactivity on blots generated by hybridization with 32P-cDNA probes was evaluated and compared with screen-enhanced X-Ray Film autoradiography. Quantitation of RNA dot blots hybridized with a 28S ribosomal RNA-specific cDNA probe showed that storage phosphor imaging was more sensitive than screen-enhanced X-Ray Film autoradiography in identifying low amounts of total RNA (1-10 micrograms). Evaluation of Northern blots containing 30 micrograms of total RNA from human skin biopsies hybridized with a cDNA probe for the human acidic ribosomal phosphoprotein, PO, showed that both techniques detected random biological variability of this housekeeping gene in a similar manner. The two techniques eXhibited a strong linear correlation in their ability to quantitate mRNA levels of a retinoic acid-inducible gene (RIS-1). This correlation was stronger at levels corresponding to 1-fold to 30-fold increases of signal and decreased beyond this range because of the insensitivity of the X-Ray Film. In conclusion, storage phosphor imaging is more accurate than screen-enhanced X-Ray Film autoradiography in identifying different RNA amounts (higher sensitivity) and in detecting increasing RNA signals at high levels of radioactivity (higher dynamic range).
Haci Deveci - One of the best experts on this subject based on the ideXlab platform.
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recovery of silver from X Ray Film processing effluents by hydrogen peroXide treatment
Hydrometallurgy, 2012Co-Authors: Ahmet Deniz Bas, Ersin Y Yazici, Haci DeveciAbstract:article i nfo In this study, recovery of silver from X-Ray Film processing effluents by precipitation was studied. Hydrogen peroXide was used asthe precipitating agent. A fullfactorialdesign (4 2 ) approach was adopted for the study. The results have shown that precipitation process is highly eXothermic in nature with the evolution of copious amount of heat apparently owing to the concomitant oXidation of thiosulphate. The precipitation of silver by hydrogen peroXide is a fast reaction, which is almost complete within minutes. It is also an acid consuming reactionwiththe tendency of pHtoincreasetowards neutral/alkaline conditions. The concentration ofhydrogen peroXide was proved to be statistically the most significant factor affecting the precipitation process. High silver recoveries(≥95%)from thewaste solution (1.1 g/L Ag, 113 g/L S2O3 2 − ) were obtained only at high levels of H2O2 (≥37.6 g/L). Over the reaction period, a substantial increase in the concentration of sulphate was noted indicating the consumption of H2O2 mainly by the oXidation of thiosulphate. Increasing pH was found to have a beneficial effect on the recovery of silver noticeably at low H2O2 concentrations. The addition of ethylene glycol (0.5-10 mL) enhanced the recovery of Ag (by 1.3-18.7%) presumably due to its stabilising effect on H2O2 .S EM- EDS and XRD analysesof theprecipitateshave revealed thatsilverispresent mainlyas finesilversulphide. These findings demonstrate that the waste photoprocessing solutions can be suitably treated by hydrogen peroXide to recover silver and remove thiosulphate.
Franz Léon Burny - One of the best experts on this subject based on the ideXlab platform.
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Does the normal contralateral wrist provide the best reference for X-Ray Film measurements of the patholgoic wrist?
The Journal of hand surgery, 1996Co-Authors: Frederic Schuind, S. Alemzadeh, Bernard Stallenberg, Franz Léon BurnyAbstract:This study compares in normal subjects the variability of wrist X-Ray Film measurements between the right and left sides with the variability of the distribution of those measurements within the population. Additional purposes were to evaluate possible differences of these measurements according to seX, age, side, and hand dominance. The variability when comparing both sides was found to be statistically less for the carpal height, radiolunate, scapholunate, and capitolunate angles than within the whole group of subjects, and for those measurements, there was a high correlation ( r >.88) between the right and left sides. In addition, the carpal height and the carporadial ratios were lower and the capitolunate angle was higher in women than in men. There was also a significant decrease of the scapholunate angle in older individuals. The clinical implication of these findings is that in unilateral wrist diseases, the normal wrist should be used to provide the reference values of the carpal height and of the carpal angles on profile X-Ray Films. However, for the radial inclination and palmar tilt of the distal radius and for the ulnar variance, the normal side does not provide a better reference than normal values obtained from databases.
John A Groves - One of the best experts on this subject based on the ideXlab platform.
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Chemical pollutants in X-Ray Film processing departments.
Annals of Occupational Hygiene, 1996Co-Authors: Emma Scobbie, David William Dabill, John A GrovesAbstract:Abstract A survey of X-Ray Film processing departments revealed the main airborne contaminants to be sulphur dioXide and acetic acid at concentrations of about 0.1 ppm. Glutaraldehyde was not detected either in the ambient air or in the eXhaust duct from an automatic Film processor. Laboratory studies confirmed sulphur dioXide and acetic acid as the main headspace constituents above working strength processing solutions but glutaraldehyde and butyraldehyde were also detected.
Christos C. Zouboulis - One of the best experts on this subject based on the ideXlab platform.
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Storage phosphor imaging technique improves the accuracy of RNA quantitation using 32P-labeled cDNA probes.
BioTechniques, 1994Co-Authors: Christos C. Zouboulis, Amir TavakkolAbstract:The use of the storage phosphor imaging technique to quantitate radioactivity on blots generated by hybridization with 32P-cDNA probes was evaluated and compared with screen-enhanced X-Ray Film autoradiography. Quantitation of RNA dot blots hybridized with a 28S ribosomal RNA-specific cDNA probe showed that storage phosphor imaging was more sensitive than screen-enhanced X-Ray Film autoradiography in identifying low amounts of total RNA (1-10 micrograms). Evaluation of Northern blots containing 30 micrograms of total RNA from human skin biopsies hybridized with a cDNA probe for the human acidic ribosomal phosphoprotein, PO, showed that both techniques detected random biological variability of this housekeeping gene in a similar manner. The two techniques eXhibited a strong linear correlation in their ability to quantitate mRNA levels of a retinoic acid-inducible gene (RIS-1). This correlation was stronger at levels corresponding to 1-fold to 30-fold increases of signal and decreased beyond this range because of the insensitivity of the X-Ray Film. In conclusion, storage phosphor imaging is more accurate than screen-enhanced X-Ray Film autoradiography in identifying different RNA amounts (higher sensitivity) and in detecting increasing RNA signals at high levels of radioactivity (higher dynamic range).
