The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Dimitri Heintz - One of the best experts on this subject based on the ideXlab platform.
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Xenobiotics metabolization in Salix alba leaves uncovered by mass spectrometry imaging
Metabolomics, 2019Co-Authors: Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri HeintzAbstract:Introduction Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. Objectives This study aimed at understanding the role of plants in Xenobiotics removal from polluted environments by following Xenobiotics metabolism in leaf tissues. Methods Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of Xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). Results The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage Xenobiotics uptake from a polluted environment. Conclusion Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
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Xenobiotics metabolization in salix alba leaves uncovered by mass spectrometry imaging
Metabolomics, 2019Co-Authors: Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri HeintzAbstract:INTRODUCTION: Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. OBJECTIVES: This study aimed at understanding the role of plants in Xenobiotics removal from polluted environments by following Xenobiotics metabolism in leaf tissues. METHODS: Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of Xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). RESULTS: The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage Xenobiotics uptake from a polluted environment. CONCLUSION: Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
Loïc Maurer - One of the best experts on this subject based on the ideXlab platform.
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Xenobiotics metabolization in Salix alba leaves uncovered by mass spectrometry imaging
Metabolomics, 2019Co-Authors: Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri HeintzAbstract:Introduction Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. Objectives This study aimed at understanding the role of plants in Xenobiotics removal from polluted environments by following Xenobiotics metabolism in leaf tissues. Methods Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of Xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). Results The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage Xenobiotics uptake from a polluted environment. Conclusion Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
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Xenobiotics metabolization in salix alba leaves uncovered by mass spectrometry imaging
Metabolomics, 2019Co-Authors: Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri HeintzAbstract:INTRODUCTION: Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. OBJECTIVES: This study aimed at understanding the role of plants in Xenobiotics removal from polluted environments by following Xenobiotics metabolism in leaf tissues. METHODS: Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of Xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). RESULTS: The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage Xenobiotics uptake from a polluted environment. CONCLUSION: Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
Claire Villette - One of the best experts on this subject based on the ideXlab platform.
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Xenobiotics metabolization in Salix alba leaves uncovered by mass spectrometry imaging
Metabolomics, 2019Co-Authors: Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri HeintzAbstract:Introduction Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. Objectives This study aimed at understanding the role of plants in Xenobiotics removal from polluted environments by following Xenobiotics metabolism in leaf tissues. Methods Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of Xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). Results The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage Xenobiotics uptake from a polluted environment. Conclusion Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
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Xenobiotics metabolization in salix alba leaves uncovered by mass spectrometry imaging
Metabolomics, 2019Co-Authors: Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri HeintzAbstract:INTRODUCTION: Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. OBJECTIVES: This study aimed at understanding the role of plants in Xenobiotics removal from polluted environments by following Xenobiotics metabolism in leaf tissues. METHODS: Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of Xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). RESULTS: The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage Xenobiotics uptake from a polluted environment. CONCLUSION: Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
Adrien Wanko - One of the best experts on this subject based on the ideXlab platform.
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Xenobiotics metabolization in Salix alba leaves uncovered by mass spectrometry imaging
Metabolomics, 2019Co-Authors: Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri HeintzAbstract:Introduction Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. Objectives This study aimed at understanding the role of plants in Xenobiotics removal from polluted environments by following Xenobiotics metabolism in leaf tissues. Methods Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of Xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). Results The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage Xenobiotics uptake from a polluted environment. Conclusion Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
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Xenobiotics metabolization in salix alba leaves uncovered by mass spectrometry imaging
Metabolomics, 2019Co-Authors: Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri HeintzAbstract:INTRODUCTION: Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. OBJECTIVES: This study aimed at understanding the role of plants in Xenobiotics removal from polluted environments by following Xenobiotics metabolism in leaf tissues. METHODS: Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of Xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). RESULTS: The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage Xenobiotics uptake from a polluted environment. CONCLUSION: Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
Dirk Springael - One of the best experts on this subject based on the ideXlab platform.
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horizontal gene transfer and microbial adaptation to Xenobiotics new types of mobile genetic elements and lessons from ecological studies
Trends in Microbiology, 2004Co-Authors: Dirk SpringaelAbstract:Abstract The characterization of bacteria that degrade organic Xenobiotics has revealed that they can adapt to these compounds by expressing ‘novel' catabolic pathways. At least some of them appear to have evolved by patchwork assembly of horizontally transmitted genes and subsequent mutations and gene rearrangements. Recent studies have revealed the existence of new types of xenobiotic catabolic mobile genetic elements, such as catabolic genomic islands, which integrate into the chromosome after transfer. The significance of horizontal gene transfer and patchwork assembly for bacterial adaptation to pollutants under real environmental conditions remains uncertain, but recent publications suggest that these processes do occur in a polluted environment.
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the use of bacteria immobilized in tubular membrane reactors for heavy metal recovery and degradation of chlorinated aromatics
Journal of Membrane Science, 1995Co-Authors: Ludo Diels, Dirk Springael, K Somers, I Willems, Wim Doyen, Max Mergeay, Roger LeysenAbstract:Abstract Microbial treatments of waste water can be done in membrane reactors. A membrane installed outside the reactor is used to separate bacteria from the treated effluent. A new membrane reactor concept is presented. The separation membrane is introduced in the reactor and not outside as in a normal one. The membrane plays the role of a separator of two streams and is used at the same time as the immobilizing support for the bacteria. The reactor keeps the bacteria active via a specific nutrient stream that is provided on one side of the membrane. The bacteria grow in and on the membrane where they form an active biofilm. The bacteria can treat the effluent on one side and can be kept active via the nutrient stream at the other side without contamination of the effluent by the nutrient. In this work, the performance of the BICMER (Bacteria Immobilized Composite MEmbrane Reactor) is demonstrated via treatments of effluents containing heavy metals or organic Xenobiotics. For heavy metal removal Alcaligenes eutrophus CH34 bacteria were used. These bacteria induce a metal bioprecipitation process that results in the formation of crystalline metal carbonates, which are recovered on a separate column in the reactor. In this way metals can be recovered without disturbing the biofilm on the membrane. Metals such as Cd, Zn, Cu, Pb and Y can be reduced to less than 50 ppb. The metals Co, Ni, Pd and Ge are reduced to below 100 ppb. For organic Xenobiotics Alcaligenes eutrophus AE1308 bacteria or other strains (depending on the xenobiotic to be degraded) were used. This strain degrades the xenobiotic 3-chlorobenzoate (Cba) and 2,4-dichlorophenoxyacetic acid to CO2, H2O and chloride). Concentrations of 3 mM Cba could be reduced to less than 0.1 mM. For other toxic organic compounds, different biodegrading strains need to be used.
