The Experts below are selected from a list of 210 Experts worldwide ranked by ideXlab platform
Juan Larraín - One of the best experts on this subject based on the ideXlab platform.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described. Here we present a protocol for the husbandry of Xenopus laevis tadpoles and froglets, and procedures to study spinal cord regeneration. This includes methods to induce spinal cord injury (SCI); DNA and morpholino electroporation for genetic studies; in vivo imaging for cell analysis; a swimming test to measure functional recovery; and a convenient model for screening for new compounds that promote neural regeneration. These protocols establish X. laevis as a unique model organism for understanding spinal cord regeneration by comparing regenerative and nonregenerative stages. This protocol can be used to understand the molecular and cellular mechanisms involved in nervous system regeneration, including neural stem and progenitor cell (NSPC) proliferation and neurogenesis, extrinsic and intrinsic mechanisms involved in axon regeneration, glial response and scar formation, and trophic factors. For experienced personnel, husbandry takes 1–2 months; SCI can be achieved in 5–15 min; and swimming recovery takes 20–30 d.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described.
Gabriela Edwards-faret - One of the best experts on this subject based on the ideXlab platform.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described. Here we present a protocol for the husbandry of Xenopus laevis tadpoles and froglets, and procedures to study spinal cord regeneration. This includes methods to induce spinal cord injury (SCI); DNA and morpholino electroporation for genetic studies; in vivo imaging for cell analysis; a swimming test to measure functional recovery; and a convenient model for screening for new compounds that promote neural regeneration. These protocols establish X. laevis as a unique model organism for understanding spinal cord regeneration by comparing regenerative and nonregenerative stages. This protocol can be used to understand the molecular and cellular mechanisms involved in nervous system regeneration, including neural stem and progenitor cell (NSPC) proliferation and neurogenesis, extrinsic and intrinsic mechanisms involved in axon regeneration, glial response and scar formation, and trophic factors. For experienced personnel, husbandry takes 1–2 months; SCI can be achieved in 5–15 min; and swimming recovery takes 20–30 d.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described.
Victor S. Tapia - One of the best experts on this subject based on the ideXlab platform.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described. Here we present a protocol for the husbandry of Xenopus laevis tadpoles and froglets, and procedures to study spinal cord regeneration. This includes methods to induce spinal cord injury (SCI); DNA and morpholino electroporation for genetic studies; in vivo imaging for cell analysis; a swimming test to measure functional recovery; and a convenient model for screening for new compounds that promote neural regeneration. These protocols establish X. laevis as a unique model organism for understanding spinal cord regeneration by comparing regenerative and nonregenerative stages. This protocol can be used to understand the molecular and cellular mechanisms involved in nervous system regeneration, including neural stem and progenitor cell (NSPC) proliferation and neurogenesis, extrinsic and intrinsic mechanisms involved in axon regeneration, glial response and scar formation, and trophic factors. For experienced personnel, husbandry takes 1–2 months; SCI can be achieved in 5–15 min; and swimming recovery takes 20–30 d.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described.
Emilio E. Méndez-olivos - One of the best experts on this subject based on the ideXlab platform.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described. Here we present a protocol for the husbandry of Xenopus laevis tadpoles and froglets, and procedures to study spinal cord regeneration. This includes methods to induce spinal cord injury (SCI); DNA and morpholino electroporation for genetic studies; in vivo imaging for cell analysis; a swimming test to measure functional recovery; and a convenient model for screening for new compounds that promote neural regeneration. These protocols establish X. laevis as a unique model organism for understanding spinal cord regeneration by comparing regenerative and nonregenerative stages. This protocol can be used to understand the molecular and cellular mechanisms involved in nervous system regeneration, including neural stem and progenitor cell (NSPC) proliferation and neurogenesis, extrinsic and intrinsic mechanisms involved in axon regeneration, glial response and scar formation, and trophic factors. For experienced personnel, husbandry takes 1–2 months; SCI can be achieved in 5–15 min; and swimming recovery takes 20–30 d.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described.
Rosana Muñoz - One of the best experts on this subject based on the ideXlab platform.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described. Here we present a protocol for the husbandry of Xenopus laevis tadpoles and froglets, and procedures to study spinal cord regeneration. This includes methods to induce spinal cord injury (SCI); DNA and morpholino electroporation for genetic studies; in vivo imaging for cell analysis; a swimming test to measure functional recovery; and a convenient model for screening for new compounds that promote neural regeneration. These protocols establish X. laevis as a unique model organism for understanding spinal cord regeneration by comparing regenerative and nonregenerative stages. This protocol can be used to understand the molecular and cellular mechanisms involved in nervous system regeneration, including neural stem and progenitor cell (NSPC) proliferation and neurogenesis, extrinsic and intrinsic mechanisms involved in axon regeneration, glial response and scar formation, and trophic factors. For experienced personnel, husbandry takes 1–2 months; SCI can be achieved in 5–15 min; and swimming recovery takes 20–30 d.
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Spinal cord regeneration in Xenopus laevis
Nature Protocols, 2017Co-Authors: Gabriela Edwards-faret, Dasfne Lee-liu, Victor S. Tapia, Rosana Muñoz, Emilio E. Méndez-olivos, Juan LarraínAbstract:Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described.
