The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Hiroo Fukuda - One of the best experts on this subject based on the ideXlab platform.
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Cargo-dependent and Cell wall-associated Xylem transport in Arabidopsis.
The New phytologist, 2018Co-Authors: Satoshi Endo, Yumi Iwai, Hiroo FukudaAbstract:Sap molecules are transported by Xylem flow throughout the whole plant body. Factors regulating the Xylem transport of different molecules remain to be identified. We used fluorophores to visualize Xylem transport from roots to leaves in Arabidopsis thaliana. Several previously established Arabidopsis lines with modified Xylem Cell walls were used to determine the contribution of Xylem Cell walls to Xylem transport. Fluorophores underwent Xylem flow-dependent transport from roots to leaves within 20 min. A comparison of rhodamine, fluorescein and three fluorescently labeled CLV3/ESR-related (CLE) peptides revealed cargo-dependent Xylem transport patterns in terms of leaf position and vein order. Only minor changes in amino acid sequence were sufficient to alter the Xylem transport patterns of the labeled CLE peptides. We found that the Xylem transport pattern of fluorescein was affected in Arabidopsis lines with modified AtXYN1, LAC4 or CCoAOMT1 expression. In these lines, application of a defense inducer, pipecolic acid, to roots resulted in altered defense response patterns in leaves, whereas all the lines showed wild-type-like responses when pipecolic acid was sprayed onto leaves. The combined results reveal a finely controlled cargo-dependent Xylem transport and suggest that the Xylem Cell wall structure is crucial for this transport system.
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Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Masato Saito, Yuki Kondo, Alif Meem Nurani, Hiroo FukudaAbstract:Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for Xylem Cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic Xylem Cells can be induced throughout leaf disks within 3-4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular Cell induction culture.
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Co-Regulation of Brassinosteroid Biosynthesis-Related Genes During Xylem Cell Differentiation
2016Co-Authors: Ryo Yamamoto, Kuninori Iwamoto, Taku Demura, Shozo Fujioka, Suguru Takatsuto, Shigeo Yoshida, Hiroo FukudaAbstract:To understand the regulatory mechanisms of brassino-steroid (BR) biosynthesis in specific plant developmental processes, we first investigated the accumulation profiles of BRs and sterols in Xylem differentiation in a Zinnia culture. The amounts of many substances in the late C28 sterol biosynthetic pathway to campesterol (CR), such as episterol and 24-methylenecholesterol, as well as those in the BR-specific biosynthetic pathway from CR to brassinolide (BL), were elevated in close association with tracheary element differentiation. Among them, 6-deoxotyphasterol (6-deoxoTY) accumulated to unusually high levels within Cells cultured in tracheary element-inductive medium, while castasterone (CS) was not elevated either within or outside Cells. To identify the molecular basis of this co-up-regulation of BRs and C28 sterols, we isolated Zinnia genes for the ke
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A Novel System for Xylem Cell Differentiation in Arabidopsis thaliana
Molecular plant, 2014Co-Authors: Yuki Kondo, Takashi Fujita, Munetaka Sugiyama, Hiroo FukudaAbstract:Abstract During vascular development, procambial and cambial Cells give rise to Xylem and phloem Cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular development in detail. Here, we establish a novel in vitro experimental system in which vascular development is induced in Arabidopsis thaliana leaf-disk cultures using bikinin, an inhibitor of glycogen synthase kinase 3 proteins. Transcriptome analysis reveals that mesophyll Cells in leaf disks synchronously turn into procambial Cells and then differentiate into tracheary elements. Leaf-disk cultures from plants expressing the procambial Cell markers TDR pro :GUS and TDR pro :YFP can be used for spatiotemporal visualization of procambial Cell formation. Further analysis with the tdr mutant and TDIF (tracheary element differentiation inhibitory factor) indicates that the key signaling TDIF-TDR-GSK3s regulates Xylem differentiation in leaf-disk cultures. This new culture system can be combined with analysis using the rich material resources for Arabidopsis including Cell-marker lines and mutants, thus offering a powerful tool for analyzing Xylem Cell differentiation.
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Regulation of Xylem Cell fate
Frontiers in plant science, 2014Co-Authors: Yuki Kondo, Takayuki Tamaki, Hiroo FukudaAbstract:The vascular system is deliberately placed throughout the plant body for transporting water, nutrients, and signaling molecules. During vascular development, Xylem, phloem, and procambial/cambial Cells are differentiated in a spatiotemporally organized manner. Up to now, various key regulators for Xylem Cell patterning and differentiation such as auxin, cytokinin, CLE peptides, microRNAs, HD-ZIPIIIs, VNDs, and moving transcription factors SHR and AHLs have been discovered. Recent studies are revealing functional interactions among such factors to eventually determine the Xylem Cell fate. This review focuses on networks of various regulators underlying Xylem Cell fate determination in root vascular development.
Yuki Kondo - One of the best experts on this subject based on the ideXlab platform.
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Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Masato Saito, Yuki Kondo, Alif Meem Nurani, Hiroo FukudaAbstract:Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for Xylem Cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic Xylem Cells can be induced throughout leaf disks within 3-4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular Cell induction culture.
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A Novel System for Xylem Cell Differentiation in Arabidopsis thaliana
Molecular plant, 2014Co-Authors: Yuki Kondo, Takashi Fujita, Munetaka Sugiyama, Hiroo FukudaAbstract:Abstract During vascular development, procambial and cambial Cells give rise to Xylem and phloem Cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular development in detail. Here, we establish a novel in vitro experimental system in which vascular development is induced in Arabidopsis thaliana leaf-disk cultures using bikinin, an inhibitor of glycogen synthase kinase 3 proteins. Transcriptome analysis reveals that mesophyll Cells in leaf disks synchronously turn into procambial Cells and then differentiate into tracheary elements. Leaf-disk cultures from plants expressing the procambial Cell markers TDR pro :GUS and TDR pro :YFP can be used for spatiotemporal visualization of procambial Cell formation. Further analysis with the tdr mutant and TDIF (tracheary element differentiation inhibitory factor) indicates that the key signaling TDIF-TDR-GSK3s regulates Xylem differentiation in leaf-disk cultures. This new culture system can be combined with analysis using the rich material resources for Arabidopsis including Cell-marker lines and mutants, thus offering a powerful tool for analyzing Xylem Cell differentiation.
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Regulation of Xylem Cell fate
Frontiers in plant science, 2014Co-Authors: Yuki Kondo, Takayuki Tamaki, Hiroo FukudaAbstract:The vascular system is deliberately placed throughout the plant body for transporting water, nutrients, and signaling molecules. During vascular development, Xylem, phloem, and procambial/cambial Cells are differentiated in a spatiotemporally organized manner. Up to now, various key regulators for Xylem Cell patterning and differentiation such as auxin, cytokinin, CLE peptides, microRNAs, HD-ZIPIIIs, VNDs, and moving transcription factors SHR and AHLs have been discovered. Recent studies are revealing functional interactions among such factors to eventually determine the Xylem Cell fate. This review focuses on networks of various regulators underlying Xylem Cell fate determination in root vascular development.
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plant gsk3 proteins regulate Xylem Cell differentiation downstream of tdif tdr signalling
Nature Communications, 2014Co-Authors: Yuki Kondo, Takayuki Tamaki, Tasuku Ito, Hirofumi Nakagami, Yuki Hirakawa, Masato Saito, Ken Shirasu, Hiroo FukudaAbstract:The differentiation of procambial Cells into Xylem Cells during plant radial growth is regulated by the TDIF–TDR signalling pathway. Here, the authors show that GSK3 protein kinases and their target transcription factor BES1 act downstream of TDIF–TDR signalling during Xylem Cell differentiation in Arabidopsis.
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Plant GSK3 proteins regulate Xylem Cell differentiation downstream of TDIF–TDR signalling
Nature communications, 2014Co-Authors: Yuki Kondo, Takayuki Tamaki, Tasuku Ito, Hirofumi Nakagami, Yuki Hirakawa, Masato Saito, Ken Shirasu, Hiroo FukudaAbstract:The differentiation of procambial Cells into Xylem Cells during plant radial growth is regulated by the TDIF–TDR signalling pathway. Here, the authors show that GSK3 protein kinases and their target transcription factor BES1 act downstream of TDIF–TDR signalling during Xylem Cell differentiation in Arabidopsis.
Judith Felten - One of the best experts on this subject based on the ideXlab platform.
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an ap2 erf transcription factor erf139 coordinates Xylem Cell expansion and secondary Cell wall deposition
New Phytologist, 2019Co-Authors: Bernard Wessels, Carolin Seyfferth, Jorma Vahala, Jaakko Kangasjarvi, Michaela Eder, Nicolas Delhomme, Sacha Escamez, Thomas Vain, Kamil Antos, Judith FeltenAbstract:Differentiation of Xylem elements involves Cell expansion, secondary Cell wall (SCW) deposition and programmed Cell death. Transitions between these phases require strict spatiotemporal control. The function of Populus ERF139 (Potri.013G101100) in Xylem differentiation was characterized in transgenic overexpression and dominant repressor lines of ERF139 in hybrid aspen (Populus tremula × tremuloides). Xylem properties, SCW chemistry and downstream targets were analyzed in both types of transgenic trees using microscopy techniques, Fourier transform-infrared spectroscopy, pyrolysis-GC/MS, wet chemistry methods and RNA sequencing. Opposite phenotypes were observed in the secondary Xylem vessel sizes and SCW chemistry in the two different types of transgenic trees, supporting the function of ERF139 in suppressing the radial expansion of vessel elements and stimulating accumulation of guaiacyl-type lignin and possibly also xylan. Comparative transcriptomics identified genes related to SCW biosynthesis (LAC5, LBD15, MYB86) and salt and drought stress-responsive genes (ANAC002, ABA1) as potential direct targets of ERF139. The phenotypes of the transgenic trees and the stem expression profiles of ERF139 potential target genes support the role of ERF139 as a transcriptional regulator of Xylem Cell expansion and SCW formation, possibly in response to osmotic changes of the Cells.
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An AP2/ERF transcription factor ERF139 coordinates Xylem Cell expansion and secondary Cell wall deposition.
The New phytologist, 2019Co-Authors: Bernard Wessels, Carolin Seyfferth, Jorma Vahala, Jaakko Kangasjarvi, Michaela Eder, Nicolas Delhomme, Sacha Escamez, Thomas Vain, Kamil Antos, Judith FeltenAbstract:Differentiation of Xylem elements involves Cell expansion, secondary Cell wall (SCW) deposition and programmed Cell death. Transitions between these phases require strict spatiotemporal control. The function of Populus ERF139 (Potri.013G101100) in Xylem differentiation was characterized in transgenic overexpression and dominant repressor lines of ERF139 in hybrid aspen (Populus tremula × tremuloides). Xylem properties, SCW chemistry and downstream targets were analyzed in both types of transgenic trees using microscopy techniques, Fourier transform-infrared spectroscopy, pyrolysis-GC/MS, wet chemistry methods and RNA sequencing. Opposite phenotypes were observed in the secondary Xylem vessel sizes and SCW chemistry in the two different types of transgenic trees, supporting the function of ERF139 in suppressing the radial expansion of vessel elements and stimulating accumulation of guaiacyl-type lignin and possibly also xylan. Comparative transcriptomics identified genes related to SCW biosynthesis (LAC5, LBD15, MYB86) and salt and drought stress-responsive genes (ANAC002, ABA1) as potential direct targets of ERF139. The phenotypes of the transgenic trees and the stem expression profiles of ERF139 potential target genes support the role of ERF139 as a transcriptional regulator of Xylem Cell expansion and SCW formation, possibly in response to osmotic changes of the Cells.
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populus erf85 mediates the transition between Xylem Cell expansion and secondary Cell wall formation in hybrid aspen
2018Co-Authors: Carolin Seyfferth, Hannele Tuominen, Bernard Wessels, Jorma Vahala, Jaakko Kangasjarvi, G Bauer, Michaela Eder, Nicolas Delhomme, Judith FeltenAbstract:Populus ERF85 mediates the transition between Xylem Cell expansion and secondary Cell wall formation in hybrid aspen
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Ethylene-Related Gene Expression Networks in Wood Formation
Frontiers Media S.A., 2018Co-Authors: Carolin Seyfferth, Bernard Wessels, Nicolas Delhomme, Judith Felten, Soile Jokipii-lukkari, Björn Sundberg, Hannele TuominenAbstract:Thickening of tree stems is the result of secondary growth, accomplished by the meristematic activity of the vascular cambium. Secondary growth of the stem entails developmental cascades resulting in the formation of secondary phloem outwards and secondary Xylem (i.e., wood) inwards of the stem. Signaling and transcriptional reprogramming by the phytohormone ethylene modifies cambial growth and Cell differentiation, but the molecular link between ethylene and secondary growth remains unknown. We addressed this shortcoming by analyzing expression profiles and co-expression networks of ethylene pathway genes using the AspWood transcriptome database which covers all stages of secondary growth in aspen (Populus tremula) stems. ACC synthase expression suggests that the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is synthesized during Xylem expansion and Xylem Cell maturation. Ethylene-mediated transcriptional reprogramming occurs during all stages of secondary growth, as deduced from AspWood expression profiles of ethylene-responsive genes. A network centrality analysis of the AspWood dataset identified EIN3D and 11 ERFs as hubs. No overlap was found between the co-expressed genes of the EIN3 and ERF hubs, suggesting target diversification and hence independent roles for these transcription factor families during normal wood formation. The EIN3D hub was part of a large co-expression gene module, which contained 16 transcription factors, among them several new candidates that have not been earlier connected to wood formation and a VND-INTERACTING 2 (VNI2) homolog. We experimentally demonstrated Populus EIN3D function in ethylene signaling in Arabidopsis thaliana. The ERF hubs ERF118 and ERF119 were connected on the basis of their expression pattern and gene co-expression module composition to Xylem Cell expansion and secondary Cell wall formation, respectively. We hereby establish data resources for ethylene-responsive genes and potential targets for EIN3D and ERF transcription factors in Populus stem tissues, which can help to understand the range of ethylene targeted biological processes during secondary growth
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2018Co-Authors: Carolin Seyfferth, Bernard Wessels, Nicolas Delhomme, Judith Felten, Soile Jokipii-lukkari, Björn Sundberg, Hannele TuominenAbstract:Thickening of tree stems is the result of secondary growth, accomplished by the meristematic activity of the vascular cambium. Secondary growth of the stem entails developmental cascades resulting in the formation of secondary phloem outwards and secondary Xylem (i.e., wood) inwards of the stem. Signaling and transcriptional reprogramming by the phytohormone ethylene modifies cambial growth and Cell differentiation, but the molecular link between ethylene and secondary growth remains unknown. We addressed this shortcoming by analyzing expression profiles and co-expression networks of ethylene pathway genes using the AspWood transcriptome database which covers all stages of secondary growth in aspen (Populus tremula) stems. ACC synthase expression suggests that the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is synthesized during Xylem expansion and Xylem Cell maturation. Ethylene-mediated transcriptional reprogramming occurs during all stages of secondary growth, as deduced from AspWood expression profiles of ethylene-responsive genes. A network centrality analysis of the AspWood dataset identified EIN3D and 11 ERFs as hubs. No overlap was found between the co-expressed genes of the EIN3 and ERF hubs, suggesting target diversification and hence independent roles for these transcription factor families during normal wood formation. The EIN3D hub was part of a large co-expression gene module, which contained 16 transcription factors, among them several new candidates that have not been earlier connected to wood formation and a VND-INTERACTING 2 (VNI2) homolog. We experimentally demonstrated Populus EIN3D function in ethylene signaling in Arabidopsis thaliana. The ERF hubs ERF118 and ERF119 were connected on the basis of their expression pattern and gene co-expression module composition to Xylem Cell expansion and secondary Cell wall formation, respectively. We hereby establish data resources for ethylene-responsive genes and potential targets for EIN3D and ERF transcription factors in Populus stem tissues, which can help to understand the range of ethylene targeted biological processes during secondary growth.
Yoshihisa Oda - One of the best experts on this subject based on the ideXlab platform.
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VND6-induced Xylem Cell Differentiation in Arabidopsis Cell Cultures.
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Yoshihisa OdaAbstract:In vitro Xylem differentiation is a powerful technique that can be used to elucidate the process of Xylem development that occurs deep inside plant tissues in nature. The experimental procedure described here is designed to induce metaXylem vessel differentiation at exceptionally high frequency and synchronicity using genetically engineered Arabidopsis Cell suspensions. By triggering a transcriptional switch, over 80 % of the Cells synchronously differentiate into Xylem Cells within 32 h of treatment with estradiol. Exogenous marker genes can be transiently introduced into the Cells by coculturing them with transformed Agrobacterium before inducing Xylem differentiation. This system is fast, easy to handle, and highly compatible with molecular and Cell biology techniques used to explore Xylem Cell differentiation.
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Spatial organization of Xylem Cell walls by ROP GTPases and microtubule-associated proteins.
Current opinion in plant biology, 2013Co-Authors: Yoshihisa Oda, Hiroo FukudaAbstract:Proper patterning of Cellulosic Cell walls is critical for Cell shaping and differentiation of plant Cells. Cortical microtubule arrays regulate the deposition patterns of Cellulose microfibrils by controlling the targeting and trajectory of Cellulose synthase complexes. Although some microtubule-associated proteins (MAPs) regulate the arrangement of cortical microtubules, knowledge about the overall mechanism governing the spacing of cortical microtubules is still limited. Recent studies reveal that ROP GTPases and MAPs spatially regulate the assembly and disassembly of cortical microtubules in developing Xylem Cells, in which localized secondary Cell walls are deposited. Here, we review recent insights into the regulation of Xylem Cell wall patterning by cortical microtubules, ROP GTPases, and MAPs.
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secondary Cell wall patterning during Xylem differentiation
Current Opinion in Plant Biology, 2012Co-Authors: Yoshihisa Oda, Hiroo FukudaAbstract:Xylem Cell differentiation involves temporal and spatial regulation of secondary Cell wall deposition. The cortical microtubules are known to regulate the spatial pattern of the secondary Cell wall by orientating Cellulose deposition. However, it is largely unknown how the microtubule arrangement is regulated during secondary wall formation. Recent findings of novel plant microtubule-associated proteins in developing Xylem vessels shed new light on the regulation mechanism of the microtubule arrangement leading to secondary wall patterning. In addition, in vitro culture systems allow the dynamics of microtubules and microtubule-associated proteins during secondary Cell wall formation to be followed. Therefore, this review focuses on novel aspects of microtubule dynamics leading to secondary Cell wall patterning with a focus on microtubule-associated proteins.
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wood Cell wall structure requires local 2d microtubule disassembly by a novel plasma membrane anchored protein
Current Biology, 2010Co-Authors: Yoshihisa Oda, Yuki Kondo, Yuki Iida, Hiroo FukudaAbstract:Plant Cells have evolved cortical microtubules, in a two-dimensional space beneath the plasma membrane, that regulate patterning of Cellulose deposition. Although recent studies have revealed that several microtubule-associated proteins facilitate self-organization of transverse cortical microtubules, it is still unknown how diverse patterns of cortical microtubules are organized in different Xylem Cells, which are the major components of wood. Using our newly established in vitro Xylem Cell differentiation system, we found that a novel microtubule end-tracking protein, microtubule depletion domain 1 (MIDD1), was anchored to distinct plasma membrane domains and promoted local microtubule disassembly, resulting in pits on Xylem Cell walls. The introduction of RNA interference for MIDD1 resulted in the failure of local microtubule depletion and the formation of secondary walls without pits. Conversely, the overexpression of MIDD1 reduced microtubule density. MIDD1 has two coiled-coil domains for the binding to microtubules and for the anchorage to plasma membrane domains, respectively. Combination of the two coils caused end tracking of microtubules during shrinkage and suppressed their rescue events. Our results indicate that MIDD1 integrates spatial information in the plasma membrane with cortical microtubule dynamics for determining Xylem Cell wall pattern.
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Cytoskeletal organization during Xylem Cell differentiation
Journal of Plant Research, 2006Co-Authors: Yoshihisa Oda, Seiichiro HasezawaAbstract:The water and mineral conductive tube, the Xylem vessel and tracheid, is a highly conspicuous tissue due to its elaborately patterned secondary-wall deposition. One constituent of the Xylem vessel and tracheid, the tracheary element, is an empty dead Cell that develops secondary walls in the elaborate patterns. The wall pattern is appropriately regulated according to the developmental stage of the plant. The cytoskeleton is an essential component of this regulation. In fact, the cortical microtubule is well known to participate in patterned secondary Cell wall formation. The dynamic rearrangement of the microtubules and actin filaments have also been recognized in the cultured Cells differentiating into tracheary elements in vitro. There has recently been considerable progress in our understanding of the dynamics and regulation of cortical microtubules, and several plant microtubule associated proteins have been identified and characterized. The microtubules have been observed during tracheary element differentiation in living Arabidopsis thaliana Cells. Based on this recently acquired information on the plant cytoskeleton and tracheary element differentiation, this review discusses the role of the cytoskeleton in secondary Cell wall formation.
Masato Saito - One of the best experts on this subject based on the ideXlab platform.
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Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Masato Saito, Yuki Kondo, Alif Meem Nurani, Hiroo FukudaAbstract:Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for Xylem Cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic Xylem Cells can be induced throughout leaf disks within 3-4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular Cell induction culture.
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plant gsk3 proteins regulate Xylem Cell differentiation downstream of tdif tdr signalling
Nature Communications, 2014Co-Authors: Yuki Kondo, Takayuki Tamaki, Tasuku Ito, Hirofumi Nakagami, Yuki Hirakawa, Masato Saito, Ken Shirasu, Hiroo FukudaAbstract:The differentiation of procambial Cells into Xylem Cells during plant radial growth is regulated by the TDIF–TDR signalling pathway. Here, the authors show that GSK3 protein kinases and their target transcription factor BES1 act downstream of TDIF–TDR signalling during Xylem Cell differentiation in Arabidopsis.
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Plant GSK3 proteins regulate Xylem Cell differentiation downstream of TDIF–TDR signalling
Nature communications, 2014Co-Authors: Yuki Kondo, Takayuki Tamaki, Tasuku Ito, Hirofumi Nakagami, Yuki Hirakawa, Masato Saito, Ken Shirasu, Hiroo FukudaAbstract:The differentiation of procambial Cells into Xylem Cells during plant radial growth is regulated by the TDIF–TDR signalling pathway. Here, the authors show that GSK3 protein kinases and their target transcription factor BES1 act downstream of TDIF–TDR signalling during Xylem Cell differentiation in Arabidopsis.
