Xylooligosaccharides

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Nanik Rahmani - One of the best experts on this subject based on the ideXlab platform.

  • gh 10 and gh 11 endo 1 4 β xylanase enzymes from kitasatospora sp produce xylose and Xylooligosaccharides from sugarcane bagasse with no xylose inhibition
    Bioresource Technology, 2019
    Co-Authors: Nanik Rahmani, Prihardi Kahar, Puspita Lisdiyanti, Jaemin Lee, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo
    Abstract:

    A novel strategy for the low-cost, high-yield co-production of xylose and Xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and Xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced Xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.

  • Pemurnian Parsial dan Karakterisasi Enzim Xilanase dari Bakteri Laut Bacillus safencis strain LBF P20 Asal Pulau Pari Jakarta
    Universitas Gadjah Mada, 2017
    Co-Authors: Fitria Fitria, Nanik Rahmani, Sri Pujiyanto, Budi Raharjo, Yopi Yopi
    Abstract:

    Enzyme xylanase (EC 3.2.1.8) is widely used in various industrial  fields for the hydrolysis of xylan (hemicellulose) into xylooligosaccharide and xylose. The aims of this study were to  conduct partial purification and characterization of xylanase from marine Bacillus safencis strain LBF P20 and to obtain the  xylooligosaccharide types from xylan hydrolysis by this enzyme.  Based on this research, the optimum time for enzyme production  occurred at 96 hours with the enzyme activity of 6.275 U/mL and  enzyme specific activity of 5.093 U/mg. The specific activities were  obtained from precipitation by amicon® ultra-15 centrifugal filter devices, gel filtration chromatography and anion exchange chromatography that were increased by 15.07, 34.7, and 96.0  U/mg. The results showed that the highest activity at pH 7, temperature of 60 °C, and stable at 4 °C. Type of  xylooligosaccharide produced by this study were xylohexoses, xylotriose, and xylobiose. SDS-PAGE analysis and zimogram  showed that the molecular weight of xylanase protein were about  25 kDa.   ABSTRAK Enzim xilanase (EC 3.2.1.8) digunakan dalam hidrolisis xilan  (hemiselulosa) menjadi xilooligosakarida dan xilosa. Penelitian  ini bertujuan untuk melakukan purifikasi parsial dan karakterisasi xilanase dari bakteri laut Bacillus safencis strain LBF P20 serta uji  hidrolisis untuk mengetahui jenis xilooligosakarida yang  dihasilkan oleh enzim tersebut. Berdasarkan hasil penelitian, waktu optimum untuk produksi enzim terjadi pada jam ke 96  dengan aktivitas enzim sebesar 6,275 U/mL dan aktivitas spesifik enzim sebesar 5,093 (U/mg). Aktivitas spesifik enzim hasil  pemekatan dengan amicon® ultra-15 centrifugal filter devices,  kromatografi filtrasi gel dan kromatografi penukar anion  mengalami peningkatan berturut-turut sebesar 15,1; 34,7 dan96,0 U/mg. Hasil karakterisasi menunjukkan aktivitas  tertinggi pada pH 7, suhu 60 °C dan stabil pada suhu 4 °C. Analisis SDS-PAGE dan zimogram menunjukkan berat molekul protein xilanase berkisar 25 kDa. Jenis gula reduksi yang  dihasilkan yaitu xiloheksosa, xilotriosa, dan xilobiosa

Yopi Yopi - One of the best experts on this subject based on the ideXlab platform.

  • Pemurnian Parsial dan Karakterisasi Enzim Xilanase dari Bakteri Laut Bacillus safencis strain LBF P20 Asal Pulau Pari Jakarta
    Universitas Gadjah Mada, 2017
    Co-Authors: Fitria Fitria, Nanik Rahmani, Sri Pujiyanto, Budi Raharjo, Yopi Yopi
    Abstract:

    Enzyme xylanase (EC 3.2.1.8) is widely used in various industrial  fields for the hydrolysis of xylan (hemicellulose) into xylooligosaccharide and xylose. The aims of this study were to  conduct partial purification and characterization of xylanase from marine Bacillus safencis strain LBF P20 and to obtain the  xylooligosaccharide types from xylan hydrolysis by this enzyme.  Based on this research, the optimum time for enzyme production  occurred at 96 hours with the enzyme activity of 6.275 U/mL and  enzyme specific activity of 5.093 U/mg. The specific activities were  obtained from precipitation by amicon® ultra-15 centrifugal filter devices, gel filtration chromatography and anion exchange chromatography that were increased by 15.07, 34.7, and 96.0  U/mg. The results showed that the highest activity at pH 7, temperature of 60 °C, and stable at 4 °C. Type of  xylooligosaccharide produced by this study were xylohexoses, xylotriose, and xylobiose. SDS-PAGE analysis and zimogram  showed that the molecular weight of xylanase protein were about  25 kDa.   ABSTRAK Enzim xilanase (EC 3.2.1.8) digunakan dalam hidrolisis xilan  (hemiselulosa) menjadi xilooligosakarida dan xilosa. Penelitian  ini bertujuan untuk melakukan purifikasi parsial dan karakterisasi xilanase dari bakteri laut Bacillus safencis strain LBF P20 serta uji  hidrolisis untuk mengetahui jenis xilooligosakarida yang  dihasilkan oleh enzim tersebut. Berdasarkan hasil penelitian, waktu optimum untuk produksi enzim terjadi pada jam ke 96  dengan aktivitas enzim sebesar 6,275 U/mL dan aktivitas spesifik enzim sebesar 5,093 (U/mg). Aktivitas spesifik enzim hasil  pemekatan dengan amicon® ultra-15 centrifugal filter devices,  kromatografi filtrasi gel dan kromatografi penukar anion  mengalami peningkatan berturut-turut sebesar 15,1; 34,7 dan96,0 U/mg. Hasil karakterisasi menunjukkan aktivitas  tertinggi pada pH 7, suhu 60 °C dan stabil pada suhu 4 °C. Analisis SDS-PAGE dan zimogram menunjukkan berat molekul protein xilanase berkisar 25 kDa. Jenis gula reduksi yang  dihasilkan yaitu xiloheksosa, xilotriosa, dan xilobiosa

Francisco M Girio - One of the best experts on this subject based on the ideXlab platform.

  • production purification and characterisation of oligosaccharides from olive tree pruning autohydrolysis
    Industrial Crops and Products, 2012
    Co-Authors: Cristóbal Cara, Florbela Carvalheiro, Eulogio Castro, Ignacio Ballesteros, Encarnacion Ruiz, Patricia Moura, Francisco M Girio
    Abstract:

    Abstract The production of oligosaccharides (OS) by olive tree pruning autohydrolysis in the range 170–230 °C was studied. The best results in terms of maximum yield of OS along with a low amount of byproducts were obtained at 180 °C. After purification by preparative gel filtration chromatography a range of OS-fractions with average degree of polymerisation (DP) from 25 to 3 was selected for further characterisation. Gluco- and Xylooligosaccharides were the predominant OS in these fractions. OS yields in the range 80–90% were obtained for fractions with average DP between 25 and 7, practically free of low molecular compounds. Both OS total yields and Xylooligosaccharides proportion decreased for lower DP fractions while monosaccharides and other products concentrations increased. OS production and the recovery of other high value compounds can be envisaged as an interesting contribution to develop an olive-biomass biorefinery.

  • wheat straw autohydrolysis process optimization and products characterization
    Applied Biochemistry and Biotechnology, 2009
    Co-Authors: Florbela Carvalheiro, Talita Silvafernandes, Luis C Duarte, Francisco M Girio
    Abstract:

    Wheat straw was subjected to autohydrolysis treatments in order to selectively hydrolyze the hemicellulose fraction. The effects of temperature (150–240°C) and non-isothermal reaction time on the composition of both liquid and solid phases were evaluated and interpreted using the severity factor (log R 0). The operational conditions leading to the maximum recovery of hemicellulose-derived sugars were established for log R 0 = 3.96 and correspond to 64% of the original (arabino)xylan with 80% of sugars as Xylooligosaccharides. Under these conditions, a solubilization of 58% xylan, 83% arabinan, and 98% acetyl groups occurred. Glucan was mainly retained in the solid phase (maximum solubilization 16%), which enables an enrichment of the solid phase to contain up to 61% glucan. Delignification was not extensive, being utmost 15%. The yields of soluble products, including sugars, acetic acid, and degradation compounds, such as, furfural, 5-hydroxymethylfurfural furfural obtained suggest the fitness of liquid stream for fermentation purposes or to obtain Xylooligosaccharides with potential applications in food, pharmaceutical, and cosmetic industries.

  • wheat straw autohydrolysis process optimization and products characterization
    Applied Biochemistry and Biotechnology, 2009
    Co-Authors: Florbela Carvalheiro, Talita Silvafernandes, Luis C Duarte, Francisco M Girio
    Abstract:

    Wheat straw was subjected to autohydrolysis treatments in order to selectively hydrolyze the hemicellulose fraction. The effects of temperature (150-240 degrees C) and non-isothermal reaction time on the composition of both liquid and solid phases were evaluated and interpreted using the severity factor (log R0). The operational conditions leading to the maximum recovery of hemicellulose-derived sugars were established for log R0 = 3.96 and correspond to 64% of the original (arabino)xylan with 80% of sugars as Xylooligosaccharides. Under these conditions, a solubilization of 58% xylan, 83% arabinan, and 98% acetyl groups occurred. Glucan was mainly retained in the solid phase (maximum solubilization 16%), which enables an enrichment of the solid phase to contain up to 61% glucan. Delignification was not extensive, being utmost 15%. The yields of soluble products, including sugars, acetic acid, and degradation compounds, such as, furfural, 5-hydroxymethylfurfural furfural obtained suggest the fitness of liquid stream for fermentation purposes or to obtain Xylooligosaccharides with potential applications in food, pharmaceutical, and cosmetic industries.

Mária Vršanská - One of the best experts on this subject based on the ideXlab platform.

  • synthesis and hydrolysis of 1 3 β xylosidic linkages by endo 1 4 β xylanase of cryptococcus albidus
    FEBS Journal, 2005
    Co-Authors: Mária Vršanská
    Abstract:

    Purified extracellular endo-1,4-β-xylanase (EC 3.2.1.8) of the yeast Cryptococcus albidus was found to catalyze not only the known 1,4-β-transfer, but an alternative transglycosylation reaction leading to the formation of 1,3-β-glycosidic linkages. From a mixture of products of β-xylanase degradation of phenyl β-D-xylopyranoside three xylooligosaccharide fractions, differring chromatographically from the 1,4-β-linked products, were isolated by preparative paper chromatography. Their structure was elucidated by mass spectrometry, 13C-NMR spectroscopy and enzymic hydrolysis by β-xylanase and β-xylosidase. The isomeric xylotriose was identified as 3-O-β-D-xylopyranosyl-4-O-β-D-xylopyranosyl-D-xylose. The fraction of isomeric tetrasaccharides was found to be represented mainly by 4-O-β-D-xylopyranosyl-3-O-β-D-xylopyranosyl-4-O-β-D-xylopyranosyl-D-xylose. The Xylooligosaccharides containing one 1,3-β-linkage were also produced on the enzyme treatment of 1,4-β-xylotriose and 1,4-β-xylan. When treated with the enzyme responsible for their synthesis, the isomeric Xylooligosaccharides were hydrolyzed at the 1,3-β-linkage, despite the fact the enzyme does not attack 1,3-β-xylan. The results are interpreted in the relation to the characterized four-subsite substrate-binding site of the enzyme.

  • biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of sporotrichum thermophile
    Carbohydrate Research, 2003
    Co-Authors: Mária Vršanská, Marc Claeyssens, Petros Katapodis, Dimitris Kekos, Wim Nerinckx, Basil J Macris, Paul Christakopoulos
    Abstract:

    An endo-beta-1,4-xylanase (1,4-beta-D-xylan xylanoxydrolase, EC 3.2.1.8) present in culture filtrates of Sporotrichum thermophile ATCC 34628 was purified to homogeneity by Q-Sepharose and Sephacryl S-200 column chromatographies. The enzyme has a molecular mass of 25,000 Da, an isoelectric point of 6.7, and is optimally active at pH 5 and at 70 degrees C. Thin-layer chromatography (TLC) analysis showed that endo-xylanase liberates mainly xylose (Xyl) and xylobiose (Xyl2) from beechwood 4-O-methyl-D-glucuronoxylan, O-acetyl-4-O-methylglucuronoxylan and rhodymenan (a beta-(1-->4)-beta(1-->3)-xylan). Also, the enzyme releases an acidic xylo-oligosaccharide from 4-O-methyl-D-glucuronoxylan, and an isomeric xylotetraose and an isomeric xylopentaose from rhodymenan. Analysis of reaction mixtures by high performance liquid chromatography (HPLC) revealed that the enzyme cleaves preferentially the internal glycosidic bonds of Xylooligosaccharides, [1-3H]-Xylooligosaccharides and xylan. The enzyme also hydrolyses the 4-methylumbelliferyl glycosides of beta-xylobiose and beta-xylotriose at the second glycosidic bond adjacent to the aglycon. The endoxylanase is not active on pNPX and pNPC. The enzyme mediates a decrease in the viscosity of xylan associated with a release of only small amounts of reducing sugar. The enzyme is irreversibly inhibited by series of omega-epoxyalkyl glycosides of D-xylopyranose. The results suggest that the endoxylanase from S. thermophile has catalytic properties similar to the enzymes belonging to family 11.

L R Rodrigues - One of the best experts on this subject based on the ideXlab platform.

  • one step process for producing prebiotic arabino Xylooligosaccharides from brewer s spent grain employing trichoderma species
    Food Chemistry, 2019
    Co-Authors: Claudia Amorim, Sara C Silverio, L R Rodrigues
    Abstract:

    Abstract Xylooligosaccharides (XOS) are prebiotic nutraceuticals that can be sourced from lignocellulosic biomass, such as agro-residues. This study reports for the first time an optimization study of XOS production from agro-residues by direct fermentation using two Trichoderma species. A total of 13 residues were evaluated as potential substrates for single-step production. The best results were found for Trichoderma reesei using brewers’ spent grain (BSG) as substrate. Under optimal conditions (3 days, pH 7.0, 30 °C and 20 g/L of BSG), a production yield of 38.3 ± 1.8 mg/g (xylose equivalents/g of BSG) was achieved. The obtained oligosaccharides were identified as arabino-xylooligosacharides (AXOS) with degree of polymerization from 2 to 5. One-step fermentation proved to be a promising strategy for AXOS production from BSG, presenting a performance comparable with the use of commercial enzymes. This study provides new insights towards the bioprocess integration, enabling further developments of low-cost bioprocesses for the production of these valuable compounds.