The Experts below are selected from a list of 1647 Experts worldwide ranked by ideXlab platform
Miriam Martino - One of the best experts on this subject based on the ideXlab platform.
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corn starch systems as carriers for Yerba Mate ilex paraguariensis antioxidants
Food and Bioproducts Processing, 2015Co-Authors: Lorena Deladino, Alba S. Navarro, Aline Schneider Teixeira, Inmaculada Alvarez, Antonio D Molinagarcia, Miriam MartinoAbstract:Abstract Corn starch's ability to bind and carry a Yerba Mate extract with strong antioxidant properties was explored in this paper. The starch was treated by high hydrostatic pressure to increase its loading capacity and scanning electron microscopy revealed that the high pressure treatment altered the shape of starch granules and changed their surface appearance. High pressure caused partial granule gelatinization as determined by calorimetric measurements, also increasing the granule specific surface area, as quantified by nitrogen adsorption. This increase in surface was due to the generation of pores, which favored the adsorption of the Yerba Mate hydrosoluble polyphenols. The Yerba Mate polyphenol concentration in the starch carriers was determined by HPLC-MS and its antioxidant activity was measured by the DPPH radical and photochemiluminiscense (PCL) methods. Treated starches incorporated a higher amount of Yerba Mate polyphenols, however, native starch could be an appropriate antioxidant carrier as well. Furthermore, it was found that the antioxidant activity was maintained after high pressure treatment without changing the Yerba Mate polyphenols profile.
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Yerba Mate antioxidant powders obtained by co-crystallization: Stability during storage
Journal of Food Engineering, 2014Co-Authors: Alex López-córdoba, Lorena Deladino, Leidy Agudelo-mesa, Miriam MartinoAbstract:Abstract The interest on Yerba Mate has increased in the last years due to its high content of bioactive compounds related to health benefits. Antioxidant aqueous extracts of Yerba Mate were entrapped into a sucrose matrix by co-crystallization. The products were characterized in terms of their morphology, entrapment yield, loading capacity, water activity, moisture content, antioxidant activity, thermal behavior, solubility and hygroscopicity. It was found that the co-crystallization process led to high entrapment yield and maintained the antioxidant activity of the Yerba Mate extract towards DPPH radical. Also, the co-crystallized powders showed values of water activity, moisture content, hygroscopicity and flowability indicative of high stability and good handling properties. The total polyphenols content of the co-crystallized products remained almost constant along storage at 75% RH and 20 °C, however, fluctuations in their DPPH radical scavenging activity were observed.
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Release of Yerba Mate antioxidants from corn starch-alginate capsules as affected by structure.
Carbohydrate polymers, 2013Co-Authors: Alex López-córdoba, Lorena Deladino, Miriam MartinoAbstract:Abstract Encapsulation of Yerba Mate ( Ilex paraguariensis ) extract in a proper matrix enhances the possible applications of this natural antioxidant in food systems. To start, calcium alginate capsules were used as carriers of Yerba Mate extract and a filler Material (corn starch at 2%) was added to the alginate matrix to improve the structural properties and to modulate the release of the active compounds. Next, kinetics and swelling mechanisms involved in the release of Yerba Mate polyphenols in simulated digestive fluids were analyzed. A lower rate of release was obtained with calcium alginate–starch capsules as compared to control ones, which was attributed to the lower porosity of filled capsules. The release profiles of both systems were satisfactorily fitted with semi-empirical models, which indicated that a combined mechanism of polymer-chain relaxation and diffusion was taking place.
Marcelo Lima Ribeiro - One of the best experts on this subject based on the ideXlab platform.
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Yerba Mate (Ilex paraguariensis) inhibits lymphocyte activation in vitro
Food & function, 2016Co-Authors: Maider Muñoz-culla, Marcelo Lima Ribeiro, Matías Sáenz-cuesta, Maier J. Guereca-barandiaran, David OtaeguiAbstract:Yerba Mate (YM) has been shown to have anti-inflammatory properties in several studies. However, this effect has been found mainly in obesity-related inflammation. The aim of this work was to study the effect of YM on cultured peripheral blood mononuclear cells to see whether it has anti-inflammatory properties. We stimulated peripheral blood mononuclear cells in vitro with phytohemagglutinin (PHA) in the presence of Yerba Mate and determined their activation by measuring the expression of CD25 by flow cytometry. We observed that YM treatment produced a dose-dependent reduction in PBMC activation (CD25 positive cells) when they were stimulated with PHA. This effect was also observed in T cells' (CD3 positive) subpopulation. Microarray analysis revealed the differential expression of 128 genes in YM-treated cells. According to a protein-protein interaction database, these genes were highly connected and they are involved in the inflammatory response. In summary, it was demonstrated that YM produces a reduction in the amount of activated cells under the stimulation of PHA. Therefore, it might be used in diseases with an inflammatory component.
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the positive effects of Yerba Mate ilex paraguariensis in obesity
Nutrients, 2015Co-Authors: Alessandra Gambero, Marcelo Lima RibeiroAbstract:The prevalence of obesity has increased worldwide over the past three decades. Global anti-obesity strategies focus on dietary and lifestyle modifications to slow the development of obesity. Research in the nutrition field has recently aroused considerable interest based on the potential of natural products to counteract obesity. Several studies have identified Yerba Mate (Ilex paraguariensis) as an excellent candidate. In this review, we evaluated the impact of Yerba Mate on obesity and obesity-related inflammation. Cellular studies demonstrate that Yerba Mate suppresses adipocyte differentiation and triglyceride accumulation and reduces inflammation. Animal studies show that Yerba Mate modulates signaling pathways that regulate adipogenesis, antioxidant, anti-inflammatory and insulin signaling responses. In summary, the data presented here showed that the use of Yerba Mate might be useful against obesity, improving the lipid parameters in humans and animal models. In addition, Yerba Mate modulates the expression of genes that are changed in the obese state and restores them to more normal levels of expression. In doing so, it addresses several of the abnormal and disease-causing factors associated with obesity. Protective and ameliorative effects on insulin resistance were also observed. Thus, as a general conclusion, it seems that Yerba Mate beverages and supplements might be helpful in the battle against obesity.
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effects of Yerba Mate a plant extract formulation ygd and resveratrol in 3t3 l1 adipogenesis
Molecules, 2014Co-Authors: Juliana Carvalho Santos, Alessandra Gambero, Erica Martins Ferreira Gotardo, Mitsue T Brianti, Mahmood Piraee, Marcelo Lima RibeiroAbstract:We aimed to evaluate the in vitro effects of Yerba Mate, YGD (a herbal preparation containing Yerba Mate, guarana and damiana), and resveratrol on adipogenesis. The anti-adipogenic effects of Yerba Mate, YGD, resveratrol and YGD + resveratrol and Yerba Mate + resveratrol combinations were evaluated in 3T3-L1 cells by Oil Red staining, cellular triglyceride content, and PCR quantitative array. The results demonstrated that all of the tested compounds inhibited adipogenesis. Yerba Mate extract significantly down-regulated the expression of genes that play an important role in regulating adipogenesis, such as Adig, Axin, Cebpa, Fgf10, Lep, Lpl, and Pparγ2. In addition, these genes, YGD also repressed Bmp2, Ccnd1, Fasn, and Srebf1. Resveratrol also modulated the expression of Adig, Bmp2, Ccnd1, C/EBPα, Fasn, Fgf10, Lep, Lpl, and Pparγ2. Moreover, resveratrol repressed Cebpb, Cdk4, Fgf2, and Klf15. The Yerba Mate extract and YGD up-regulated the expression of genes involved in inhibiting adipogenesis, such as Dlk-1, Klf2, and Ucp1. Resveratrol also induced the expression of Klf2 and Ucp1. In addition resveratrol modulated the Ddit3, Foxo1, Sirt1, and Sirt2. The combined effects of these compounds on gene expression showed similar results observed from individual treatments. Our data indicates that the synergy between the compounds favors the inhibition of adipogenesis.
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the in vitro and in vivo effects of Yerba Mate ilex paraguariensis extract on adipogenesis
Food Chemistry, 2013Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Marcelo Lima Ribeiro, Juliana Carvalho SantosAbstract:The aim of this study was to evaluate the effects of Yerba Mate extract and its principal bioactive compounds on adipogenesis. The anti-adipogenic effects of Yerba Mate, chlorogenic acid, quercetin and rutin were evaluated in 3T3-L1 cells using a PCR array. The results obtained in vitro were validated in vivo in a high-fat diet-induced model of obesity. The in vitro and in vivo results demonstrated that Yerba Mate extract down-regulated the expression of genes that regulate adipogenesis, such as Creb-1and C/EBPα, and the extract up-regulated the expression of genes related to the inhibition of adipogenesis, including Dlk1, Gata2, Gata3, Klf2, Lrp5, Pparγ2, Sfrp1, Tcf7l2, Wnt10b, and Wnt3a. In summary, it was demonstrated that Yerba Mate and its bioactive compounds regulate the expression of genes related to in vitro adipogenesis. Furthermore, Yerba Mate might regulate adipogenesis through the Wnt pathway.
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modulatory effects of Yerba Mate ilex paraguariensis on the pi3k akt signaling pathway
Molecular Nutrition & Food Research, 2013Co-Authors: Alessandra Gambero, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Juliana Carvalho Santos, Lucio Fabio Caldas FerrazAbstract:The aim of this study was to evaluate the effects of Yerba Mate (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or Yerba Mate extract at 1.0 g/kg. After treatment, glucose blood level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that Yerba Mate extract significantly decreased the final body weight, glucose blood levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after Yerba Mate treatment, the levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of Yerba Mate on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.
Lorena Deladino - One of the best experts on this subject based on the ideXlab platform.
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biodegradation of Yerba Mate waste based fertilizer capsules effect of temperature
Journal of Polymers and The Environment, 2019Co-Authors: Lenin Llive, E Bruno, A D Molinagarcia, A Schneiderteixeira, Lorena DeladinoAbstract:Yerba Mate waste based urea fertilizer capsules were obtained and characterized. Yerba Mate powder (YMP) content in these encapsulation systems ranged from 52 to 82% for the different formulations. The aim of the study was to evaluate the effect of Yerba Mate powder:calcium alginate ratio and the assay temperature on capsule degradation and urea release rate in soil. Degradation evolution was monitored by microbiological tests, environmental scanning electronic microscopy, differential scanning calorimetry and Fourier transform infrared spectrometry. Encapsulation efficiency increased with YMP content. The green fertilizing systems generated did not inhibit microorganism development in soil. Degradation was slower for capsules with higher YMP content. Capsules with high YMP content released lower amounts of urea. Both degradation and release rates increased with assay temperature. The recycling of Yerba Mate powder as a green composite Material originated a new fertilizer system.
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Yerba Mate (Ilex paraguariensis) Waste and Alginate as a Matrix for the Encapsulation of N Fertilizer
ACS Sustainable Chemistry & Engineering, 2016Co-Authors: Aline Schneider Teixeira, Lorena Deladino, Noemí ZaritzkyAbstract:In the present work, the recycling of Yerba Mate (Ilex paraguariensis) industrial byproducts into urea-containing capsules is described as an environmentally friendly strategy for nitrogen release into the soil. Yerba Mate is an infusion traditionally drunk in various countries of South America. An extensive physical characterization of the capsules is reported: scanning electronic microscopy (SEM) and energy dispersive X-rays analysis (EDX), water gain and hygroscopicity, texture properties, measurement of water absorbency, differential scanning calorimetry (DSC), and Fourier transform infrared spectrometry (FT-IR). The capsule matrix had a Yerba Mate powder content ranging from 30% to 77%. Nitrogen release from the capsule-fertilizer and the capsule degradation were studied using soil (∼pH 7.50). All of the results indicate that Yerba Mate powder capsules with urea, as an example of an environmental friendly fertilizer, may be expected to have wide applications for the sustainable development of modern ...
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corn starch systems as carriers for Yerba Mate ilex paraguariensis antioxidants
Food and Bioproducts Processing, 2015Co-Authors: Lorena Deladino, Alba S. Navarro, Aline Schneider Teixeira, Inmaculada Alvarez, Antonio D Molinagarcia, Miriam MartinoAbstract:Abstract Corn starch's ability to bind and carry a Yerba Mate extract with strong antioxidant properties was explored in this paper. The starch was treated by high hydrostatic pressure to increase its loading capacity and scanning electron microscopy revealed that the high pressure treatment altered the shape of starch granules and changed their surface appearance. High pressure caused partial granule gelatinization as determined by calorimetric measurements, also increasing the granule specific surface area, as quantified by nitrogen adsorption. This increase in surface was due to the generation of pores, which favored the adsorption of the Yerba Mate hydrosoluble polyphenols. The Yerba Mate polyphenol concentration in the starch carriers was determined by HPLC-MS and its antioxidant activity was measured by the DPPH radical and photochemiluminiscense (PCL) methods. Treated starches incorporated a higher amount of Yerba Mate polyphenols, however, native starch could be an appropriate antioxidant carrier as well. Furthermore, it was found that the antioxidant activity was maintained after high pressure treatment without changing the Yerba Mate polyphenols profile.
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Yerba Mate antioxidant powders obtained by co-crystallization: Stability during storage
Journal of Food Engineering, 2014Co-Authors: Alex López-córdoba, Lorena Deladino, Leidy Agudelo-mesa, Miriam MartinoAbstract:Abstract The interest on Yerba Mate has increased in the last years due to its high content of bioactive compounds related to health benefits. Antioxidant aqueous extracts of Yerba Mate were entrapped into a sucrose matrix by co-crystallization. The products were characterized in terms of their morphology, entrapment yield, loading capacity, water activity, moisture content, antioxidant activity, thermal behavior, solubility and hygroscopicity. It was found that the co-crystallization process led to high entrapment yield and maintained the antioxidant activity of the Yerba Mate extract towards DPPH radical. Also, the co-crystallized powders showed values of water activity, moisture content, hygroscopicity and flowability indicative of high stability and good handling properties. The total polyphenols content of the co-crystallized products remained almost constant along storage at 75% RH and 20 °C, however, fluctuations in their DPPH radical scavenging activity were observed.
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Release of Yerba Mate antioxidants from corn starch-alginate capsules as affected by structure.
Carbohydrate polymers, 2013Co-Authors: Alex López-córdoba, Lorena Deladino, Miriam MartinoAbstract:Abstract Encapsulation of Yerba Mate ( Ilex paraguariensis ) extract in a proper matrix enhances the possible applications of this natural antioxidant in food systems. To start, calcium alginate capsules were used as carriers of Yerba Mate extract and a filler Material (corn starch at 2%) was added to the alginate matrix to improve the structural properties and to modulate the release of the active compounds. Next, kinetics and swelling mechanisms involved in the release of Yerba Mate polyphenols in simulated digestive fluids were analyzed. A lower rate of release was obtained with calcium alginate–starch capsules as compared to control ones, which was attributed to the lower porosity of filled capsules. The release profiles of both systems were satisfactorily fitted with semi-empirical models, which indicated that a combined mechanism of polymer-chain relaxation and diffusion was taking place.
Alessandra Gambero - One of the best experts on this subject based on the ideXlab platform.
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the positive effects of Yerba Mate ilex paraguariensis in obesity
Nutrients, 2015Co-Authors: Alessandra Gambero, Marcelo Lima RibeiroAbstract:The prevalence of obesity has increased worldwide over the past three decades. Global anti-obesity strategies focus on dietary and lifestyle modifications to slow the development of obesity. Research in the nutrition field has recently aroused considerable interest based on the potential of natural products to counteract obesity. Several studies have identified Yerba Mate (Ilex paraguariensis) as an excellent candidate. In this review, we evaluated the impact of Yerba Mate on obesity and obesity-related inflammation. Cellular studies demonstrate that Yerba Mate suppresses adipocyte differentiation and triglyceride accumulation and reduces inflammation. Animal studies show that Yerba Mate modulates signaling pathways that regulate adipogenesis, antioxidant, anti-inflammatory and insulin signaling responses. In summary, the data presented here showed that the use of Yerba Mate might be useful against obesity, improving the lipid parameters in humans and animal models. In addition, Yerba Mate modulates the expression of genes that are changed in the obese state and restores them to more normal levels of expression. In doing so, it addresses several of the abnormal and disease-causing factors associated with obesity. Protective and ameliorative effects on insulin resistance were also observed. Thus, as a general conclusion, it seems that Yerba Mate beverages and supplements might be helpful in the battle against obesity.
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effects of Yerba Mate a plant extract formulation ygd and resveratrol in 3t3 l1 adipogenesis
Molecules, 2014Co-Authors: Juliana Carvalho Santos, Alessandra Gambero, Erica Martins Ferreira Gotardo, Mitsue T Brianti, Mahmood Piraee, Marcelo Lima RibeiroAbstract:We aimed to evaluate the in vitro effects of Yerba Mate, YGD (a herbal preparation containing Yerba Mate, guarana and damiana), and resveratrol on adipogenesis. The anti-adipogenic effects of Yerba Mate, YGD, resveratrol and YGD + resveratrol and Yerba Mate + resveratrol combinations were evaluated in 3T3-L1 cells by Oil Red staining, cellular triglyceride content, and PCR quantitative array. The results demonstrated that all of the tested compounds inhibited adipogenesis. Yerba Mate extract significantly down-regulated the expression of genes that play an important role in regulating adipogenesis, such as Adig, Axin, Cebpa, Fgf10, Lep, Lpl, and Pparγ2. In addition, these genes, YGD also repressed Bmp2, Ccnd1, Fasn, and Srebf1. Resveratrol also modulated the expression of Adig, Bmp2, Ccnd1, C/EBPα, Fasn, Fgf10, Lep, Lpl, and Pparγ2. Moreover, resveratrol repressed Cebpb, Cdk4, Fgf2, and Klf15. The Yerba Mate extract and YGD up-regulated the expression of genes involved in inhibiting adipogenesis, such as Dlk-1, Klf2, and Ucp1. Resveratrol also induced the expression of Klf2 and Ucp1. In addition resveratrol modulated the Ddit3, Foxo1, Sirt1, and Sirt2. The combined effects of these compounds on gene expression showed similar results observed from individual treatments. Our data indicates that the synergy between the compounds favors the inhibition of adipogenesis.
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the in vitro and in vivo effects of Yerba Mate ilex paraguariensis extract on adipogenesis
Food Chemistry, 2013Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Marcelo Lima Ribeiro, Juliana Carvalho SantosAbstract:The aim of this study was to evaluate the effects of Yerba Mate extract and its principal bioactive compounds on adipogenesis. The anti-adipogenic effects of Yerba Mate, chlorogenic acid, quercetin and rutin were evaluated in 3T3-L1 cells using a PCR array. The results obtained in vitro were validated in vivo in a high-fat diet-induced model of obesity. The in vitro and in vivo results demonstrated that Yerba Mate extract down-regulated the expression of genes that regulate adipogenesis, such as Creb-1and C/EBPα, and the extract up-regulated the expression of genes related to the inhibition of adipogenesis, including Dlk1, Gata2, Gata3, Klf2, Lrp5, Pparγ2, Sfrp1, Tcf7l2, Wnt10b, and Wnt3a. In summary, it was demonstrated that Yerba Mate and its bioactive compounds regulate the expression of genes related to in vitro adipogenesis. Furthermore, Yerba Mate might regulate adipogenesis through the Wnt pathway.
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modulatory effects of Yerba Mate ilex paraguariensis on the pi3k akt signaling pathway
Molecular Nutrition & Food Research, 2013Co-Authors: Alessandra Gambero, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Juliana Carvalho Santos, Lucio Fabio Caldas FerrazAbstract:The aim of this study was to evaluate the effects of Yerba Mate (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or Yerba Mate extract at 1.0 g/kg. After treatment, glucose blood level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that Yerba Mate extract significantly decreased the final body weight, glucose blood levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after Yerba Mate treatment, the levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of Yerba Mate on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.
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antiobesity effects of Yerba Mate extract ilex paraguariensis in high fat diet induced obese mice
Obesity, 2009Co-Authors: Demetrius Paiva Arcari, Waldemar Bartchewsky, Tanila Wood Dos Santos, Karim A Oliveira, Alexandre Funck, Jose Pedrazzoli, Marina Figueiredo Ferreira De Souza, Mario J A Saad, Deborah H M Bastos, Alessandra GamberoAbstract:Because the potential of Yerba Mate (Ilex paraguariensis) has been suggested in the management of obesity, the aim of the present study was to evaluate the effects of Yerba Mate extract on weight loss, obesity-related biochemical parameters, and the regulation of adipose tissue gene expression in high-fat diet-induced obesity in mice. Thirty animals were randomly assigned to three groups. The mice were introduced to standard or high-fat diets. After 12 weeks on a high-fat diet, mice were randomly assigned according to the treatment (water or Yerba Mate extract 1.0 g/kg). After treatment intervention, plasma concentrations of total cholesterol, high-density lipoprotein cholesterol, triglyceride, low-density lipoprotein (LDL) cholesterol, and glucose were evaluated. Adipose tissue was examined to determine the mRNA levels of several genes such as tumor necrosis factor-alpha (TNF-alpha), leptin, interleukin-6 (IL-6), C-C motif chemokine ligand-2 (CCL2), CCL receptor-2 (CCR2), angiotensinogen, plasminogen activator inhibitor-1 (PAI-1), adiponectin, resistin, peroxisome proliferator-activated receptor-gamma(2) (PPAR-gamma(2)), uncoupling protein-1 (UCP1), and PPAR-gamma coactivator-1 alpha (PGC-1 alpha). The F4/80 levels were determined by immunoblotting. We found that obese mice treated with Yerba Mate exhibited marked attenuation of weight gain, adiposity, a decrease in epididymal fat-pad weight, and restoration of the serum levels of cholesterol, triglycerides, LDL cholesterol, and glucose. The gene and protein expression levels were directly regulated by the high-fat diet. After treatment with Yerba Mate extract, we observed a recovery of the expression levels. In conclusion, our data show that Yerba Mate extract has potent antiobesity activity in vivo. Additionally, we observed that the treatment had a modulatory effect on the expression of several genes related to obesity.
Demetrius Paiva Arcari - One of the best experts on this subject based on the ideXlab platform.
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the in vitro and in vivo effects of Yerba Mate ilex paraguariensis extract on adipogenesis
Food Chemistry, 2013Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Marcelo Lima Ribeiro, Juliana Carvalho SantosAbstract:The aim of this study was to evaluate the effects of Yerba Mate extract and its principal bioactive compounds on adipogenesis. The anti-adipogenic effects of Yerba Mate, chlorogenic acid, quercetin and rutin were evaluated in 3T3-L1 cells using a PCR array. The results obtained in vitro were validated in vivo in a high-fat diet-induced model of obesity. The in vitro and in vivo results demonstrated that Yerba Mate extract down-regulated the expression of genes that regulate adipogenesis, such as Creb-1and C/EBPα, and the extract up-regulated the expression of genes related to the inhibition of adipogenesis, including Dlk1, Gata2, Gata3, Klf2, Lrp5, Pparγ2, Sfrp1, Tcf7l2, Wnt10b, and Wnt3a. In summary, it was demonstrated that Yerba Mate and its bioactive compounds regulate the expression of genes related to in vitro adipogenesis. Furthermore, Yerba Mate might regulate adipogenesis through the Wnt pathway.
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modulatory effects of Yerba Mate ilex paraguariensis on the pi3k akt signaling pathway
Molecular Nutrition & Food Research, 2013Co-Authors: Alessandra Gambero, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Juliana Carvalho Santos, Lucio Fabio Caldas FerrazAbstract:The aim of this study was to evaluate the effects of Yerba Mate (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or Yerba Mate extract at 1.0 g/kg. After treatment, glucose blood level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that Yerba Mate extract significantly decreased the final body weight, glucose blood levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after Yerba Mate treatment, the levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of Yerba Mate on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.
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antiobesity effects of Yerba Mate extract ilex paraguariensis in high fat diet induced obese mice
Obesity, 2009Co-Authors: Demetrius Paiva Arcari, Waldemar Bartchewsky, Tanila Wood Dos Santos, Karim A Oliveira, Alexandre Funck, Jose Pedrazzoli, Marina Figueiredo Ferreira De Souza, Mario J A Saad, Deborah H M Bastos, Alessandra GamberoAbstract:Because the potential of Yerba Mate (Ilex paraguariensis) has been suggested in the management of obesity, the aim of the present study was to evaluate the effects of Yerba Mate extract on weight loss, obesity-related biochemical parameters, and the regulation of adipose tissue gene expression in high-fat diet-induced obesity in mice. Thirty animals were randomly assigned to three groups. The mice were introduced to standard or high-fat diets. After 12 weeks on a high-fat diet, mice were randomly assigned according to the treatment (water or Yerba Mate extract 1.0 g/kg). After treatment intervention, plasma concentrations of total cholesterol, high-density lipoprotein cholesterol, triglyceride, low-density lipoprotein (LDL) cholesterol, and glucose were evaluated. Adipose tissue was examined to determine the mRNA levels of several genes such as tumor necrosis factor-alpha (TNF-alpha), leptin, interleukin-6 (IL-6), C-C motif chemokine ligand-2 (CCL2), CCL receptor-2 (CCR2), angiotensinogen, plasminogen activator inhibitor-1 (PAI-1), adiponectin, resistin, peroxisome proliferator-activated receptor-gamma(2) (PPAR-gamma(2)), uncoupling protein-1 (UCP1), and PPAR-gamma coactivator-1 alpha (PGC-1 alpha). The F4/80 levels were determined by immunoblotting. We found that obese mice treated with Yerba Mate exhibited marked attenuation of weight gain, adiposity, a decrease in epididymal fat-pad weight, and restoration of the serum levels of cholesterol, triglycerides, LDL cholesterol, and glucose. The gene and protein expression levels were directly regulated by the high-fat diet. After treatment with Yerba Mate extract, we observed a recovery of the expression levels. In conclusion, our data show that Yerba Mate extract has potent antiobesity activity in vivo. Additionally, we observed that the treatment had a modulatory effect on the expression of several genes related to obesity.
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Yerba Mate ilex paraguariensis aqueous extract decreases intestinal sglt1 gene expression but does not affect other biochemical parameters in alloxan diabetic wistar rats
Journal of Agricultural and Food Chemistry, 2008Co-Authors: D M Oliveira, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Patricia De Oliveira Carvalho, Marina Figueiredo Ferreira De Souza, Helayne S Freitas, Deborah H M BastosAbstract:Yerba Mate (Ilex paraguariensis) is rich in polyphenols, especially chlorogenic acids. Evidence suggests that dietary polyphenols could play a role in glucose absorption and metabolism. The aim of this study was to evaluate the antidiabetic properties of Yerba Mate extract in alloxan-induced diabetic Wistar rats. Animals (n = 41) were divided in four groups: nondiabetic control (NDC, n = 10), nondiabetic Yerba Mate (NDY, n = 10), diabetic control (DC, n = 11), and diabetic Yerba Mate (DY, n = 10). The intervention consisted in the administration of Yerba Mate extract in a 1 g extract/kg body weight dose for 28 days; controls received saline solution only. There were no significant differences in serum glucose, insulin, and hepatic glucose-6-phosphatase activity between the groups that ingested Yerba Mate extract (NDY and DY) and the controls (NDC and DC). However, the intestinal SGLT1 gene expression was significantly lower in animals that received Yerba Mate both in upper (p = 0.007) and middle (p < 0.00...
