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Wieslaw Oleszek - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of oxidative stress in blood platelets by different phenolics from Yucca Schidigera Roezl. bark.
Nutrition (Burbank Los Angeles County Calif.), 2020Co-Authors: Beata Olas, Anna Stochmal, Barbara Wachowicz, Wieslaw OleszekAbstract:We investigated the comparative effects of resveratrol (trans-3,4',5-trihydroxystilbene), trans-3,3',5,5'tetrahydroxy-4'-methoxystilbene, and Yuccaols A and C isolated from the bark of Yucca Schidigera on oxidative stress in resting blood platelets and blood platelets activated by different agonists (thrombin or thrombin receptor activating peptide). Tested phenolics (1-25 microgram/mL) reduced, to different degrees, the level of reactive oxygen species measured by the luminol-dependent chemiluminescence and changed the production of O(2)(-) measured by the reduction of cytochrome c in resting blood platelets. They also inhibited the generation of free radicals in blood platelets activated by thrombin (P < 0.05) or thrombin receptor activating peptide (P < 0.05). Treatment of platelets with resveratrol or Yuccaols A and C at the concentration of 25 microgram/mL increased (statistically non-significant) the level of thiobarbituric acid reactive substances in these cells (P > 0.05), whereas trans-3,3',5,5'tetrahydroxy-4'-methoxystilbene and the alcohol Yucca extract reduced lipid peroxidation in blood platelets (P < 0.05). Resveratrol and other phenolic compounds from the bark of Yucca Schidigera inhibiting free radical generation in blood platelets may be beneficial in protecting against cardiovascular diseases when hyperactivity of platelets is observed.
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qualitative and quantitative analysis of steroidal saponins in crude extract and bark powder of Yucca Schidigera roezl
Journal of Agricultural and Food Chemistry, 2011Co-Authors: Mariusz Kowalczyk, Anna Stochmal, łukasz Pecio, Wieslaw OleszekAbstract:Steroidal saponins in commercial stem syrup and in extract of a bark of Yucca Schidigera were identified with high-performance liquid chromatography ion trap mass spectrometry and quantitated using ultraperformance liquid chromatography with quadrupole mass spectrometric detection. Fragmentation patterns of Yucca saponins were generated using collision-induced dissociation and compared with fragmentation of authentic standards as well as with published spectrometric information. In addition to detection of twelve saponins known to occur in Y. Schidigera, collected fragmentation data led to tentative identifications of seven new saponins. A quantitation method for all 19 detected compounds was developed and validated. Samples derived from the syrup and the bark of Yucca were quantitatively measured and compared. Obtained results indicate that Yucca bark accumulates polar, bidesmosidic saponins, while in the stem steroidal glycosides with middle- and short-length saccharide chains are predominant. The newly...
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influence of phenolic constituents from Yucca Schidigera bark on arachidonate metabolism in vitro
Journal of Agricultural and Food Chemistry, 2008Co-Authors: Eva Maria Wenzig, Wieslaw Oleszek, Anna Stochmal, Olaf Kunert, Rudolf BauerAbstract:Yucca Schidigera Roezl. (Agavaceae) has been traditionally used to treat a variety of diseases including arthritis and rheumatism. Phenolic constituents isolated from Yucca bark, such as resveratrol, trans-3,3′,5,5′-tetrahydroxy-4′-methoxystilbene, and the Yuccaols, have been shown to possess various activities in vitro, such as antioxidant, radical scavenging, iNOS expression inhibitory, and platelet aggregation inhibitory effects. In the present study, the influence of a phenolic-rich fraction from Yucca bark and of its main phenolic constituents on key enzymes of arachidonate metabolism was investigated. The fraction and the pure phenolics were shown to inhibit COX-1, COX-2, and LTB4 formation by 5-LOX in vitro to different extents. The degree of COX-1 inhibition was found to be strongly dependent on the substitution pattern of ring B of the stilbenic moiety. The same trend was observed for the COX-2 inhibitory potential, which was, however, in general much lower for the Yuccaols as compared with resve...
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relative effects of phenolic constituents from Yucca Schidigera roezl bark on kaposi s sarcoma cell proliferation migration and paf synthesis
Biochemical Pharmacology, 2006Co-Authors: Ciro Balestrieri, Wieslaw Oleszek, Sonia Piacente, Cosimo Pizza, Paola Montoro, Francesca Felice, Vincenzo Visciano, Maria Luisa BalestrieriAbstract:Abstract Yuccaols (A, B, C) are phenolic constituents isolated from Yucca Schidigera bark characterized by unusual spirostructures made up of a C 15 unit and a stilbenic portion closely related to resveratrol. These novel compounds are of particular interest for their antioxidant and anti-inflammatory properties. However, their effects on cell proliferation, migration, and platelet-activating factor (PAF) biosynthesis remain unknown. PAF, a potent mediator of inflammation, is known to promote angiogenesis and in vitro migration of endothelial cells and Kaposi's sarcoma (KS) cells. The objective of our study was to determine the effect of Yuccaols and resveratrol on the vascular endothelial growth factor (VEGF)-induced proliferation, migration, and PAF biosynthesis in KS cells. The results indicated that Yuccaols (25 μM) were more effective than resveratrol (25 μM) in inhibiting the VEGF-induced KS cell proliferation. Western blot analysis revealed that Yuccaols reduced the VEGF-induced phosphorylation of p38 and p42/44, thus indicating a possible interference with the mechanism underlying the VEGF-stimulated cell proliferation. Furthermore, Yuccaols completely inhibited the VEGF-stimulated PAF biosynthesis catalyzed by the acetyl-CoA:lyso-PAF acetyltransferase and enhanced its degradation through the PAF-dependent CoA-independent transacetylase (250% of control). In addition, Yuccaol C abrogated the PAF-induced cell motility whereas Yuccaol A and Yuccaol B reduced the cell migration from 7.6 μm/h to 6.1 μm/h and 5.6 μm/h, respectively. These results indicate that the anti-inflammatory properties attributed to Yucca Schidigera can be ascribed to both resveratrol and Yuccaols and provide the first evidences of the anti-tumor and anti-invasive properties of these novel phenolic compounds.
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Saponins and Phenolics of Yucca Schidigera Roezl: Chemistry and Bioactivity
Phytochemistry Reviews, 2005Co-Authors: Sonia Piacente, Cosimo Pizza, Wieslaw OleszekAbstract:Yucca Schidigera (Agavaceae) is one of the major commercial source of steroidal saponins. Two products of Yucca are available on the market. These include dried and finely powdered logs (Yucca powder) or mechanically pressed and thermally condensed juice (Yucca extract). These products possess the GRAS label which allows their use as foaming agent in soft drink (root beer), pharmaceutical, cosmetic, food, and feeding-stuffs industries. The main application of Yucca products is in animal nutrition, in particular as a feed additive to reduce ammonia and fecal odors in animal excreta. The positive effects of dietary supplementation with Yucca products on the growth rates, feed efficiency, and health of livestock seem to be due not only to the saponin constituents but also to other constituents. These observations prompted us to investigate the phenolic constituents of Y. Schidigera . This study led to the isolation of resveratrol, trans -3,3′,5,5′-tetrahydroxy-4′-methoxystilbene, the sprirobiflavonoid larixinol along with novel phenolic derivatives with very unusual spirostructures, named Yuccaols A–E and Yuccaone A. Taking into account the multifunctional activities of resveratrol and the novelty of Yuccaols A–E, structurally related to resveratrol, a program aimed to evaluate for Yucca phenolics some of the activities exerted by resveratrol has been carried out. This review describes the chemistry of Yucca saponins and phenolics, summarizes the biological activities of Yucca products and constituents and gives an account on the actual and potential applications of Yucca products.
Cosimo Pizza - One of the best experts on this subject based on the ideXlab platform.
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relative effects of phenolic constituents from Yucca Schidigera roezl bark on kaposi s sarcoma cell proliferation migration and paf synthesis
Biochemical Pharmacology, 2006Co-Authors: Ciro Balestrieri, Wieslaw Oleszek, Sonia Piacente, Cosimo Pizza, Paola Montoro, Francesca Felice, Vincenzo Visciano, Maria Luisa BalestrieriAbstract:Abstract Yuccaols (A, B, C) are phenolic constituents isolated from Yucca Schidigera bark characterized by unusual spirostructures made up of a C 15 unit and a stilbenic portion closely related to resveratrol. These novel compounds are of particular interest for their antioxidant and anti-inflammatory properties. However, their effects on cell proliferation, migration, and platelet-activating factor (PAF) biosynthesis remain unknown. PAF, a potent mediator of inflammation, is known to promote angiogenesis and in vitro migration of endothelial cells and Kaposi's sarcoma (KS) cells. The objective of our study was to determine the effect of Yuccaols and resveratrol on the vascular endothelial growth factor (VEGF)-induced proliferation, migration, and PAF biosynthesis in KS cells. The results indicated that Yuccaols (25 μM) were more effective than resveratrol (25 μM) in inhibiting the VEGF-induced KS cell proliferation. Western blot analysis revealed that Yuccaols reduced the VEGF-induced phosphorylation of p38 and p42/44, thus indicating a possible interference with the mechanism underlying the VEGF-stimulated cell proliferation. Furthermore, Yuccaols completely inhibited the VEGF-stimulated PAF biosynthesis catalyzed by the acetyl-CoA:lyso-PAF acetyltransferase and enhanced its degradation through the PAF-dependent CoA-independent transacetylase (250% of control). In addition, Yuccaol C abrogated the PAF-induced cell motility whereas Yuccaol A and Yuccaol B reduced the cell migration from 7.6 μm/h to 6.1 μm/h and 5.6 μm/h, respectively. These results indicate that the anti-inflammatory properties attributed to Yucca Schidigera can be ascribed to both resveratrol and Yuccaols and provide the first evidences of the anti-tumor and anti-invasive properties of these novel phenolic compounds.
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Saponins and Phenolics of Yucca Schidigera Roezl: Chemistry and Bioactivity
Phytochemistry Reviews, 2005Co-Authors: Sonia Piacente, Cosimo Pizza, Wieslaw OleszekAbstract:Yucca Schidigera (Agavaceae) is one of the major commercial source of steroidal saponins. Two products of Yucca are available on the market. These include dried and finely powdered logs (Yucca powder) or mechanically pressed and thermally condensed juice (Yucca extract). These products possess the GRAS label which allows their use as foaming agent in soft drink (root beer), pharmaceutical, cosmetic, food, and feeding-stuffs industries. The main application of Yucca products is in animal nutrition, in particular as a feed additive to reduce ammonia and fecal odors in animal excreta. The positive effects of dietary supplementation with Yucca products on the growth rates, feed efficiency, and health of livestock seem to be due not only to the saponin constituents but also to other constituents. These observations prompted us to investigate the phenolic constituents of Y. Schidigera . This study led to the isolation of resveratrol, trans -3,3′,5,5′-tetrahydroxy-4′-methoxystilbene, the sprirobiflavonoid larixinol along with novel phenolic derivatives with very unusual spirostructures, named Yuccaols A–E and Yuccaone A. Taking into account the multifunctional activities of resveratrol and the novelty of Yuccaols A–E, structurally related to resveratrol, a program aimed to evaluate for Yucca phenolics some of the activities exerted by resveratrol has been carried out. This review describes the chemistry of Yucca saponins and phenolics, summarizes the biological activities of Yucca products and constituents and gives an account on the actual and potential applications of Yucca products.
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liquid chromatography tandem mass spectrometry of unusual phenols from Yucca Schidigera bark comparison with other analytical techniques
Journal of Mass Spectrometry, 2004Co-Authors: Paola Montoro, Wieslaw Oleszek, Sonia Piacente, Cosimo PizzaAbstract:Qualitative and quantitative analyses of phenolic compounds are of interest for both medicinal and food plants. In the present work, the phenolic fraction from Yucca Schidigera, a plant bearing the GRAS (Generally Recognized as Safe) label approved by the US Food and Drug Administration, was studied. Crude extracts of Y. Schidigera bark were investigated by liquid chromatography/UV spectrophotometry with diode-array detection, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS), in order to develop and optimize simple and rapid techniques to determine both stilbenes and Yuccaols for the purposes of quality control of collected material. With optimal LC and MS conditions, stilbenes and Yuccaols were quantified with all the proposed methods and the results were compared. Sensitivity was evaluated and the results indicated that MS/MS detection in the multiple reaction monitoring mode is easily applicable to this plant and allows the rapid and direct identification and quantification of these peculiar compounds in crude plant extracts. Copyright © 2004 John Wiley & Sons, Ltd.
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Liquid chromatography/tandem mass spectrometry of unusual phenols from Yucca Schidigera bark: comparison with other analytical techniques
Journal of Mass Spectrometry, 2004Co-Authors: Paola Montoro, Wieslaw Oleszek, Sonia Piacente, Cosimo PizzaAbstract:Qualitative and quantitative analyses of phenolic compounds are of interest for both medicinal and food plants. In the present work, the phenolic fraction from Yucca Schidigera, a plant bearing the GRAS (Generally Recognized as Safe) label approved by the US Food and Drug Administration, was studied. Crude extracts of Y. Schidigera bark were investigated by liquid chromatography/UV spectrophotometry with diode-array detection, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS), in order to develop and optimize simple and rapid techniques to determine both stilbenes and Yuccaols for the purposes of quality control of collected material. With optimal LC and MS conditions, stilbenes and Yuccaols were quantified with all the proposed methods and the results were compared. Sensitivity was evaluated and the results indicated that MS/MS detection in the multiple reaction monitoring mode is easily applicable to this plant and allows the rapid and direct identification and quantification of these peculiar compounds in crude plant extracts. Copyright © 2004 John Wiley & Sons, Ltd.
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inhibition of inducible nitric oxide synthase expression by Yuccaol c from Yucca Schidigera roezl
Life Sciences, 2004Co-Authors: Stefania Marzocco, Wieslaw Oleszek, Anna Stochmal, Sonia Piacente, Cosimo Pizza, Aldo Pinto, Raffaella Sorrentino, Giuseppina AutoreAbstract:Abstract Yucca Schidigera extract finds wide commercial application in foods, cosmetics and pharmaceuticals. In a previous paper we have found as the main constituents of Yucca bark, Yuccaol A, B and C, new and very unusual spiro-derivatives made up of a C 15 unit and a stilbenic portion closely related to resveratrol. This study was performed to examine whether Yuccaol A, B or C (0.01–100 μM) could affect cytosolic inducible nitric oxide synthase (iNOS) protein expression and nitric oxide (NO) generation in vitro in Escherichia coli lipopolysaccharide (LPS)-activated J774.A1 macrophage cell line. NO production, detected as NO 2 − , increased significantly in LPS treated J774.A1 cells from 0.05 ± 0.03 μM to 16.64 ± 0.58 μM (P Y. Schidigera as anti-inflammatory remedy could be addressed not only to the resveratrol content but also to the presence of Yuccaol C.
Sonia Piacente - One of the best experts on this subject based on the ideXlab platform.
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relative effects of phenolic constituents from Yucca Schidigera roezl bark on kaposi s sarcoma cell proliferation migration and paf synthesis
Biochemical Pharmacology, 2006Co-Authors: Ciro Balestrieri, Wieslaw Oleszek, Sonia Piacente, Cosimo Pizza, Paola Montoro, Francesca Felice, Vincenzo Visciano, Maria Luisa BalestrieriAbstract:Abstract Yuccaols (A, B, C) are phenolic constituents isolated from Yucca Schidigera bark characterized by unusual spirostructures made up of a C 15 unit and a stilbenic portion closely related to resveratrol. These novel compounds are of particular interest for their antioxidant and anti-inflammatory properties. However, their effects on cell proliferation, migration, and platelet-activating factor (PAF) biosynthesis remain unknown. PAF, a potent mediator of inflammation, is known to promote angiogenesis and in vitro migration of endothelial cells and Kaposi's sarcoma (KS) cells. The objective of our study was to determine the effect of Yuccaols and resveratrol on the vascular endothelial growth factor (VEGF)-induced proliferation, migration, and PAF biosynthesis in KS cells. The results indicated that Yuccaols (25 μM) were more effective than resveratrol (25 μM) in inhibiting the VEGF-induced KS cell proliferation. Western blot analysis revealed that Yuccaols reduced the VEGF-induced phosphorylation of p38 and p42/44, thus indicating a possible interference with the mechanism underlying the VEGF-stimulated cell proliferation. Furthermore, Yuccaols completely inhibited the VEGF-stimulated PAF biosynthesis catalyzed by the acetyl-CoA:lyso-PAF acetyltransferase and enhanced its degradation through the PAF-dependent CoA-independent transacetylase (250% of control). In addition, Yuccaol C abrogated the PAF-induced cell motility whereas Yuccaol A and Yuccaol B reduced the cell migration from 7.6 μm/h to 6.1 μm/h and 5.6 μm/h, respectively. These results indicate that the anti-inflammatory properties attributed to Yucca Schidigera can be ascribed to both resveratrol and Yuccaols and provide the first evidences of the anti-tumor and anti-invasive properties of these novel phenolic compounds.
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Saponins and Phenolics of Yucca Schidigera Roezl: Chemistry and Bioactivity
Phytochemistry Reviews, 2005Co-Authors: Sonia Piacente, Cosimo Pizza, Wieslaw OleszekAbstract:Yucca Schidigera (Agavaceae) is one of the major commercial source of steroidal saponins. Two products of Yucca are available on the market. These include dried and finely powdered logs (Yucca powder) or mechanically pressed and thermally condensed juice (Yucca extract). These products possess the GRAS label which allows their use as foaming agent in soft drink (root beer), pharmaceutical, cosmetic, food, and feeding-stuffs industries. The main application of Yucca products is in animal nutrition, in particular as a feed additive to reduce ammonia and fecal odors in animal excreta. The positive effects of dietary supplementation with Yucca products on the growth rates, feed efficiency, and health of livestock seem to be due not only to the saponin constituents but also to other constituents. These observations prompted us to investigate the phenolic constituents of Y. Schidigera . This study led to the isolation of resveratrol, trans -3,3′,5,5′-tetrahydroxy-4′-methoxystilbene, the sprirobiflavonoid larixinol along with novel phenolic derivatives with very unusual spirostructures, named Yuccaols A–E and Yuccaone A. Taking into account the multifunctional activities of resveratrol and the novelty of Yuccaols A–E, structurally related to resveratrol, a program aimed to evaluate for Yucca phenolics some of the activities exerted by resveratrol has been carried out. This review describes the chemistry of Yucca saponins and phenolics, summarizes the biological activities of Yucca products and constituents and gives an account on the actual and potential applications of Yucca products.
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liquid chromatography tandem mass spectrometry of unusual phenols from Yucca Schidigera bark comparison with other analytical techniques
Journal of Mass Spectrometry, 2004Co-Authors: Paola Montoro, Wieslaw Oleszek, Sonia Piacente, Cosimo PizzaAbstract:Qualitative and quantitative analyses of phenolic compounds are of interest for both medicinal and food plants. In the present work, the phenolic fraction from Yucca Schidigera, a plant bearing the GRAS (Generally Recognized as Safe) label approved by the US Food and Drug Administration, was studied. Crude extracts of Y. Schidigera bark were investigated by liquid chromatography/UV spectrophotometry with diode-array detection, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS), in order to develop and optimize simple and rapid techniques to determine both stilbenes and Yuccaols for the purposes of quality control of collected material. With optimal LC and MS conditions, stilbenes and Yuccaols were quantified with all the proposed methods and the results were compared. Sensitivity was evaluated and the results indicated that MS/MS detection in the multiple reaction monitoring mode is easily applicable to this plant and allows the rapid and direct identification and quantification of these peculiar compounds in crude plant extracts. Copyright © 2004 John Wiley & Sons, Ltd.
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Liquid chromatography/tandem mass spectrometry of unusual phenols from Yucca Schidigera bark: comparison with other analytical techniques
Journal of Mass Spectrometry, 2004Co-Authors: Paola Montoro, Wieslaw Oleszek, Sonia Piacente, Cosimo PizzaAbstract:Qualitative and quantitative analyses of phenolic compounds are of interest for both medicinal and food plants. In the present work, the phenolic fraction from Yucca Schidigera, a plant bearing the GRAS (Generally Recognized as Safe) label approved by the US Food and Drug Administration, was studied. Crude extracts of Y. Schidigera bark were investigated by liquid chromatography/UV spectrophotometry with diode-array detection, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS), in order to develop and optimize simple and rapid techniques to determine both stilbenes and Yuccaols for the purposes of quality control of collected material. With optimal LC and MS conditions, stilbenes and Yuccaols were quantified with all the proposed methods and the results were compared. Sensitivity was evaluated and the results indicated that MS/MS detection in the multiple reaction monitoring mode is easily applicable to this plant and allows the rapid and direct identification and quantification of these peculiar compounds in crude plant extracts. Copyright © 2004 John Wiley & Sons, Ltd.
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inhibition of inducible nitric oxide synthase expression by Yuccaol c from Yucca Schidigera roezl
Life Sciences, 2004Co-Authors: Stefania Marzocco, Wieslaw Oleszek, Anna Stochmal, Sonia Piacente, Cosimo Pizza, Aldo Pinto, Raffaella Sorrentino, Giuseppina AutoreAbstract:Abstract Yucca Schidigera extract finds wide commercial application in foods, cosmetics and pharmaceuticals. In a previous paper we have found as the main constituents of Yucca bark, Yuccaol A, B and C, new and very unusual spiro-derivatives made up of a C 15 unit and a stilbenic portion closely related to resveratrol. This study was performed to examine whether Yuccaol A, B or C (0.01–100 μM) could affect cytosolic inducible nitric oxide synthase (iNOS) protein expression and nitric oxide (NO) generation in vitro in Escherichia coli lipopolysaccharide (LPS)-activated J774.A1 macrophage cell line. NO production, detected as NO 2 − , increased significantly in LPS treated J774.A1 cells from 0.05 ± 0.03 μM to 16.64 ± 0.58 μM (P Y. Schidigera as anti-inflammatory remedy could be addressed not only to the resveratrol content but also to the presence of Yuccaol C.
Anna Stochmal - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of oxidative stress in blood platelets by different phenolics from Yucca Schidigera Roezl. bark.
Nutrition (Burbank Los Angeles County Calif.), 2020Co-Authors: Beata Olas, Anna Stochmal, Barbara Wachowicz, Wieslaw OleszekAbstract:We investigated the comparative effects of resveratrol (trans-3,4',5-trihydroxystilbene), trans-3,3',5,5'tetrahydroxy-4'-methoxystilbene, and Yuccaols A and C isolated from the bark of Yucca Schidigera on oxidative stress in resting blood platelets and blood platelets activated by different agonists (thrombin or thrombin receptor activating peptide). Tested phenolics (1-25 microgram/mL) reduced, to different degrees, the level of reactive oxygen species measured by the luminol-dependent chemiluminescence and changed the production of O(2)(-) measured by the reduction of cytochrome c in resting blood platelets. They also inhibited the generation of free radicals in blood platelets activated by thrombin (P < 0.05) or thrombin receptor activating peptide (P < 0.05). Treatment of platelets with resveratrol or Yuccaols A and C at the concentration of 25 microgram/mL increased (statistically non-significant) the level of thiobarbituric acid reactive substances in these cells (P > 0.05), whereas trans-3,3',5,5'tetrahydroxy-4'-methoxystilbene and the alcohol Yucca extract reduced lipid peroxidation in blood platelets (P < 0.05). Resveratrol and other phenolic compounds from the bark of Yucca Schidigera inhibiting free radical generation in blood platelets may be beneficial in protecting against cardiovascular diseases when hyperactivity of platelets is observed.
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qualitative and quantitative analysis of steroidal saponins in crude extract and bark powder of Yucca Schidigera roezl
Journal of Agricultural and Food Chemistry, 2011Co-Authors: Mariusz Kowalczyk, Anna Stochmal, łukasz Pecio, Wieslaw OleszekAbstract:Steroidal saponins in commercial stem syrup and in extract of a bark of Yucca Schidigera were identified with high-performance liquid chromatography ion trap mass spectrometry and quantitated using ultraperformance liquid chromatography with quadrupole mass spectrometric detection. Fragmentation patterns of Yucca saponins were generated using collision-induced dissociation and compared with fragmentation of authentic standards as well as with published spectrometric information. In addition to detection of twelve saponins known to occur in Y. Schidigera, collected fragmentation data led to tentative identifications of seven new saponins. A quantitation method for all 19 detected compounds was developed and validated. Samples derived from the syrup and the bark of Yucca were quantitatively measured and compared. Obtained results indicate that Yucca bark accumulates polar, bidesmosidic saponins, while in the stem steroidal glycosides with middle- and short-length saccharide chains are predominant. The newly...
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influence of phenolic constituents from Yucca Schidigera bark on arachidonate metabolism in vitro
Journal of Agricultural and Food Chemistry, 2008Co-Authors: Eva Maria Wenzig, Wieslaw Oleszek, Anna Stochmal, Olaf Kunert, Rudolf BauerAbstract:Yucca Schidigera Roezl. (Agavaceae) has been traditionally used to treat a variety of diseases including arthritis and rheumatism. Phenolic constituents isolated from Yucca bark, such as resveratrol, trans-3,3′,5,5′-tetrahydroxy-4′-methoxystilbene, and the Yuccaols, have been shown to possess various activities in vitro, such as antioxidant, radical scavenging, iNOS expression inhibitory, and platelet aggregation inhibitory effects. In the present study, the influence of a phenolic-rich fraction from Yucca bark and of its main phenolic constituents on key enzymes of arachidonate metabolism was investigated. The fraction and the pure phenolics were shown to inhibit COX-1, COX-2, and LTB4 formation by 5-LOX in vitro to different extents. The degree of COX-1 inhibition was found to be strongly dependent on the substitution pattern of ring B of the stilbenic moiety. The same trend was observed for the COX-2 inhibitory potential, which was, however, in general much lower for the Yuccaols as compared with resve...
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inhibition of blood platelet adhesion and secretion by different phenolics from Yucca Schidigera roezl bark
Nutrition, 2005Co-Authors: Beata Olas, Anna Stochmal, Barbara Wachowicz, Wieslaw OleszekAbstract:Abstract Objective Yucca Schidigera is a plant that grows in Mexico, and it has a very high level of saponins and phenolic compounds with antioxidant action. The products of Y. Schidigera are used as food additives and have a generally recognized as safe label. This study investigated the antiplatelet mechanisms of four phenolic compounds. Methods We investigated antiplatelet mechanisms of the phenolic compounds trans -3,4′,5-trihydroxystilbene ( trans -resveratrol), trans -3,3′,5,5′-tetrahydroxy-4′-methoxystilbene, and Yuccaols A and C that had been isolated from the bark of Y. Schidigera by studying their effects on the first step of platelet activation, i.e., platelet adhesion to collagen and fibrinogen. The effects of these compounds on the release of adenine nucleotides, proteins, and β- N -acetyl-glycosaminidase (a marker of lysosomal secretion) from blood platelets activated by thrombin were also studied. Results These different phenolic compounds (1 to 25 μg/mL) and their extracts decreased platelet adhesion and secretion. Conclusions Resveratrol and Yucca extract were more reactive in decreasing these processes than were other tested phenolic compounds.
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Basic nutritional investigation Inhibition of blood platelet adhesion and secretion by different phenolics from Yucca Schidigera Roezl. Bark
2005Co-Authors: Beata Olas, Anna Stochmal, Barbara Wachowicz, Wieslaw OleszekAbstract:Objective: Yucca Schidigera is a plant that grows in Mexico, and it has a very high level of saponins and phenolic compounds with antioxidant action. The products of Y. Schidigera are used as food additives and have a generally recognized as safe label. This study investigated the antiplatelet mechanisms of four phenolic compounds. Methods: We investigated antiplatelet mechanisms of the phenolic compounds trans-3,4=,5- trihydroxystilbene (trans-resveratrol), trans-3,3=,5,5=-tetrahydroxy-4=-methoxystilbene, and Yucca- ols A and C that had been isolated from the bark of Y. Schidigera by studying their effects on the first step of platelet activation, i.e., platelet adhesion to collagen and fibrinogen. The effects of these compounds on the release of adenine nucleotides, proteins, and -N-acetyl-glycosaminidase (a marker of lysosomal secretion) from blood platelets activated by thrombin were also studied. Results: These different phenolic compounds (1 to 25 g/mL) and their extracts decreased platelet adhesion and secretion. Conclusions: Resveratrol and Yucca extract were more reactive in decreasing these processes than were other tested phenolic compounds. © 2005 Elsevier Inc. All rights reserved.
Paola Montoro - One of the best experts on this subject based on the ideXlab platform.
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relative effects of phenolic constituents from Yucca Schidigera roezl bark on kaposi s sarcoma cell proliferation migration and paf synthesis
Biochemical Pharmacology, 2006Co-Authors: Ciro Balestrieri, Wieslaw Oleszek, Sonia Piacente, Cosimo Pizza, Paola Montoro, Francesca Felice, Vincenzo Visciano, Maria Luisa BalestrieriAbstract:Abstract Yuccaols (A, B, C) are phenolic constituents isolated from Yucca Schidigera bark characterized by unusual spirostructures made up of a C 15 unit and a stilbenic portion closely related to resveratrol. These novel compounds are of particular interest for their antioxidant and anti-inflammatory properties. However, their effects on cell proliferation, migration, and platelet-activating factor (PAF) biosynthesis remain unknown. PAF, a potent mediator of inflammation, is known to promote angiogenesis and in vitro migration of endothelial cells and Kaposi's sarcoma (KS) cells. The objective of our study was to determine the effect of Yuccaols and resveratrol on the vascular endothelial growth factor (VEGF)-induced proliferation, migration, and PAF biosynthesis in KS cells. The results indicated that Yuccaols (25 μM) were more effective than resveratrol (25 μM) in inhibiting the VEGF-induced KS cell proliferation. Western blot analysis revealed that Yuccaols reduced the VEGF-induced phosphorylation of p38 and p42/44, thus indicating a possible interference with the mechanism underlying the VEGF-stimulated cell proliferation. Furthermore, Yuccaols completely inhibited the VEGF-stimulated PAF biosynthesis catalyzed by the acetyl-CoA:lyso-PAF acetyltransferase and enhanced its degradation through the PAF-dependent CoA-independent transacetylase (250% of control). In addition, Yuccaol C abrogated the PAF-induced cell motility whereas Yuccaol A and Yuccaol B reduced the cell migration from 7.6 μm/h to 6.1 μm/h and 5.6 μm/h, respectively. These results indicate that the anti-inflammatory properties attributed to Yucca Schidigera can be ascribed to both resveratrol and Yuccaols and provide the first evidences of the anti-tumor and anti-invasive properties of these novel phenolic compounds.
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liquid chromatography tandem mass spectrometry of unusual phenols from Yucca Schidigera bark comparison with other analytical techniques
Journal of Mass Spectrometry, 2004Co-Authors: Paola Montoro, Wieslaw Oleszek, Sonia Piacente, Cosimo PizzaAbstract:Qualitative and quantitative analyses of phenolic compounds are of interest for both medicinal and food plants. In the present work, the phenolic fraction from Yucca Schidigera, a plant bearing the GRAS (Generally Recognized as Safe) label approved by the US Food and Drug Administration, was studied. Crude extracts of Y. Schidigera bark were investigated by liquid chromatography/UV spectrophotometry with diode-array detection, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS), in order to develop and optimize simple and rapid techniques to determine both stilbenes and Yuccaols for the purposes of quality control of collected material. With optimal LC and MS conditions, stilbenes and Yuccaols were quantified with all the proposed methods and the results were compared. Sensitivity was evaluated and the results indicated that MS/MS detection in the multiple reaction monitoring mode is easily applicable to this plant and allows the rapid and direct identification and quantification of these peculiar compounds in crude plant extracts. Copyright © 2004 John Wiley & Sons, Ltd.
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Liquid chromatography/tandem mass spectrometry of unusual phenols from Yucca Schidigera bark: comparison with other analytical techniques
Journal of Mass Spectrometry, 2004Co-Authors: Paola Montoro, Wieslaw Oleszek, Sonia Piacente, Cosimo PizzaAbstract:Qualitative and quantitative analyses of phenolic compounds are of interest for both medicinal and food plants. In the present work, the phenolic fraction from Yucca Schidigera, a plant bearing the GRAS (Generally Recognized as Safe) label approved by the US Food and Drug Administration, was studied. Crude extracts of Y. Schidigera bark were investigated by liquid chromatography/UV spectrophotometry with diode-array detection, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS), in order to develop and optimize simple and rapid techniques to determine both stilbenes and Yuccaols for the purposes of quality control of collected material. With optimal LC and MS conditions, stilbenes and Yuccaols were quantified with all the proposed methods and the results were compared. Sensitivity was evaluated and the results indicated that MS/MS detection in the multiple reaction monitoring mode is easily applicable to this plant and allows the rapid and direct identification and quantification of these peculiar compounds in crude plant extracts. Copyright © 2004 John Wiley & Sons, Ltd.
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Yucca Schidigera bark phenolic constituents and antioxidant activity
Journal of Natural Products, 2004Co-Authors: Sonia Piacente, Wieslaw Oleszek, Paola Montoro, Cosimo PizzaAbstract:Two new phenolic constituents with unusual spirostructures, named Yuccaols D (1) and E (2), were isolated from the MeOH extract of Yucca Schidigera bark. Their structures were established by spectroscopic (ESIMS and NMR) analysis. The new Yuccaols D and E, along with resveratrol (3), trans-3,3‘,5,5‘-tetrahydroxy-4‘-methoxystilbene (4), Yuccaols A−C (5−7), Yuccaone A (8), larixinol (9), the MeOH extract of Yucca Schidigera bark, and the phenolic portion of this extract, were assayed for antioxidant activity by measuring the free radical scavenging effects using two different assays, namely, the Trolox Equivalent Antioxidant Capacity (TEAC) assay and the coupled oxidation of β-carotene and linoleic acid (autoxidation assay). The significant activities exhibited by the phenolic fraction and its constituents in both tests show the potential use of Y. Schidigera as a source of antioxidant principles.
