The Experts below are selected from a list of 6264966 Experts worldwide ranked by ideXlab platform
Deny Sapto Chondro Utomo - One of the best experts on this subject based on the ideXlab platform.
-
IDENTIFIKASI PLANKTON DI KAWASAN BUDIDAYA RUMPUT LAUT KABUPATEN BANTAENG, SULAWESI SELATAN DENGAN METODE DNA BARCODING
2019Co-Authors: Margaretha Sandra Apriliyanti, Sutanti Sutanti, Deny Sapto Chondro UtomoAbstract:DNA barcoding merupakan metode yang dapat digunakan untuk mengidentifikasi plankton dengan melihat materi genetiknya. DNA barcoding dilakukan dengan beberapa tahapan, yaitu ekstraksi, amplifikasi, purifikasi, dan sekuensing. Sampel yang digunakan adalah diambil pada bulan Juli 2017 dan Desember 2017 dari kawasan budidaya rumput laut Kabupaten Bantaeng, Sulawesi Selatan. Penelitian ini bertujuan untuk mengidentifikasi plankton dari kawasan budidaya rumput laut di Kabupaten Bantaeng, Sulawesi Selatan dengan metode DNA barcoding menggunakan primer 18S rDNA. Hasil penelitian menunjukkan bahwa plankton yang teridentifikasi adalah zooplankton, yaitu Pegurus bernhardus, Canthocalanus pauper, Calanus finmarchicus, dan Copepoda pada stasiun B, Acartia longiremis dan Subeucalanus pileatus pada stasiun C, dan Oithona sp. pada stasiun D dan E.
Utomo, Deny Sapto Chondro - One of the best experts on this subject based on the ideXlab platform.
-
IDENTIFIKASI PLANKTON DI KAWASAN BUDIDAYA RUMPUT LAUT KABUPATEN BANTAENG, SULAWESI SELATAN DENGAN METODE DNA BARCODING
2019Co-Authors: Apriliyanti, Margaretha Sandra, Sutanti Sutanti, Utomo, Deny Sapto ChondroAbstract:DNA barcoding is a method that can be used to identify the plankton by looking at their genetic material. DNA barcoding performed with several stages, i.e. extraction, amplification, purification, and sequencing. The sample used was taken in July 2017 and December 2017 from the area of cultivation of seaweed Bantaeng District, South Sulawesi. This study aims to identify the plankton of the area of seaweed farming in Bantaeng District, South Sulawesi with the method of DNA barcoding using the primer 18S rDNA. The results showed that the plankton identified is the zooplankton, Pegurus bernhardus, Canthocalanus pauper, Calanus finmarchicus, dan Copepoda at station B, Acartia longiremis and Subeucalanus pileatus at stations C, and Oithona sp. at stations D and E.DNA barcoding merupakan metode yang dapat digunakan untuk mengidentifikasi plankton dengan melihat materi genetiknya. DNA barcoding dilakukan dengan beberapa tahapan, yaitu ekstraksi, amplifikasi, purifikasi, dan sekuensing. Sampel yang digunakan adalah diambil pada bulan Juli 2017 dan Desember 2017 dari kawasan budidaya rumput laut Kabupaten Bantaeng, Sulawesi Selatan. Penelitian ini bertujuan untuk mengidentifikasi plankton dari kawasan budidaya rumput laut di Kabupaten Bantaeng, Sulawesi Selatan dengan metode DNA barcoding menggunakan primer 18S rDNA. Hasil penelitian menunjukkan bahwa plankton yang teridentifikasi adalah zooplankton, yaitu Pegurus bernhardus, Canthocalanus pauper, Calanus finmarchicus, dan Copepoda pada stasiun B, Acartia longiremis dan Subeucalanus pileatus pada stasiun C, dan Oithona sp. pada stasiun D dan E
Sutanti Sutanti - One of the best experts on this subject based on the ideXlab platform.
-
IDENTIFIKASI PLANKTON DI KAWASAN BUDIDAYA RUMPUT LAUT KABUPATEN BANTAENG, SULAWESI SELATAN DENGAN METODE DNA BARCODING
2019Co-Authors: Apriliyanti, Margaretha Sandra, Sutanti Sutanti, Utomo, Deny Sapto ChondroAbstract:DNA barcoding is a method that can be used to identify the plankton by looking at their genetic material. DNA barcoding performed with several stages, i.e. extraction, amplification, purification, and sequencing. The sample used was taken in July 2017 and December 2017 from the area of cultivation of seaweed Bantaeng District, South Sulawesi. This study aims to identify the plankton of the area of seaweed farming in Bantaeng District, South Sulawesi with the method of DNA barcoding using the primer 18S rDNA. The results showed that the plankton identified is the zooplankton, Pegurus bernhardus, Canthocalanus pauper, Calanus finmarchicus, dan Copepoda at station B, Acartia longiremis and Subeucalanus pileatus at stations C, and Oithona sp. at stations D and E.DNA barcoding merupakan metode yang dapat digunakan untuk mengidentifikasi plankton dengan melihat materi genetiknya. DNA barcoding dilakukan dengan beberapa tahapan, yaitu ekstraksi, amplifikasi, purifikasi, dan sekuensing. Sampel yang digunakan adalah diambil pada bulan Juli 2017 dan Desember 2017 dari kawasan budidaya rumput laut Kabupaten Bantaeng, Sulawesi Selatan. Penelitian ini bertujuan untuk mengidentifikasi plankton dari kawasan budidaya rumput laut di Kabupaten Bantaeng, Sulawesi Selatan dengan metode DNA barcoding menggunakan primer 18S rDNA. Hasil penelitian menunjukkan bahwa plankton yang teridentifikasi adalah zooplankton, yaitu Pegurus bernhardus, Canthocalanus pauper, Calanus finmarchicus, dan Copepoda pada stasiun B, Acartia longiremis dan Subeucalanus pileatus pada stasiun C, dan Oithona sp. pada stasiun D dan E
-
IDENTIFIKASI PLANKTON DI KAWASAN BUDIDAYA RUMPUT LAUT KABUPATEN BANTAENG, SULAWESI SELATAN DENGAN METODE DNA BARCODING
2019Co-Authors: Margaretha Sandra Apriliyanti, Sutanti Sutanti, Deny Sapto Chondro UtomoAbstract:DNA barcoding merupakan metode yang dapat digunakan untuk mengidentifikasi plankton dengan melihat materi genetiknya. DNA barcoding dilakukan dengan beberapa tahapan, yaitu ekstraksi, amplifikasi, purifikasi, dan sekuensing. Sampel yang digunakan adalah diambil pada bulan Juli 2017 dan Desember 2017 dari kawasan budidaya rumput laut Kabupaten Bantaeng, Sulawesi Selatan. Penelitian ini bertujuan untuk mengidentifikasi plankton dari kawasan budidaya rumput laut di Kabupaten Bantaeng, Sulawesi Selatan dengan metode DNA barcoding menggunakan primer 18S rDNA. Hasil penelitian menunjukkan bahwa plankton yang teridentifikasi adalah zooplankton, yaitu Pegurus bernhardus, Canthocalanus pauper, Calanus finmarchicus, dan Copepoda pada stasiun B, Acartia longiremis dan Subeucalanus pileatus pada stasiun C, dan Oithona sp. pada stasiun D dan E.
Stephen Brown - One of the best experts on this subject based on the ideXlab platform.
-
generalized disturbance of dna methylation in the uterine decidua in the cba j x dba 2 mouse model of pregnancy failure
2013Co-Authors: L. Brown, Elizabeth A. Bonney, Renju S. Raj, Brian W. Nielsen, Stephen BrownAbstract:Nonchromosomal pregnancy failure is a common but poorly understood phenomenon. Because recent data have suggested that epigenetic abnormalities such as abnormal placental DNA methylation may play a role in human pregnancy failure, we undertook experiments to test whether decidual and/or placental DNA methylation abnormalities are present in a mouse model of pregnancy failure. A large number of studies have shown that crosses between CBA/J female mice and DBA/2 males result in pregnancies with a high rate of failure/resorption, whereas other crosses with CBA/J females produce normal pregnancies. Although the CBA/J × DBA/2 mouse has frequently been used as a model for miscarriage, a detailed explanation for the pregnancy failure phenotype is lacking. We performed timed matings between CBA/J female and DBA/2 male mice as well as between DBA/2 female and CBA/J male mice. Decidual caps were isolated at Embryonic Day (E) 9.5 from both crosses, and a microarray-based method was used to comparatively assess genomic methylation at approximately 16 000 loci on mouse chromosome 7. In comparison with decidual caps from DBA/2 × CBA/J pregnancies, CBA/J × DBA/2 decidual caps were characterized by widely and apparently randomly disturbed methylation. In another set of analogous experiments, genomic methylation of placental DNA from E8.5 pregnancies was assessed using the same microarray-based method. This analysis revealed that in contrast to the decidua, placental DNA methylation from CBA/J × DBA/2 pregnancies was indistinguishable from that of normal controls. We conclude that abnormal DNA methylation in the uterine decidua likely plays a role in the CBA/J × DBA/2 model of pregnancy failure. To our knowledge, these experiments are the first to demonstrate that epigenetic abnormalities of the decidua are associated with pregnancy failure, and they set the stage for future efforts to understand the role of DNA methylation at the maternal-fetal interface.
-
title generalized disturbance of dna methylation in the uterine decidua in the cba j x dba 2 mouse model of pregnancy failure running title abnormal methylation in murine pregnancy failure summary sentence dna methylation in the uterine decidua of cba j x dba 2 mouse pregnancies is highly abnormal suggesting a role for epigenetics in pregnancy failure
2013Co-Authors: Lucia Y Brown, Elizabeth A. Bonney, Brian W. Nielsen, Stephen BrownAbstract:Non-chromosomal pregnancy failure is a common but poorly understood phenomenon. Because recent data have suggested that epigenetic abnormalities such as abnormal placental DNA methylation may play a role in human pregnancy failure, we undertook experiments designed to test whether decidual and/or placental DNA methylation abnormalities are present in a mouse model of pregnancy failure. A large number of studies have shown that crosses between CBA/J female mice and DBA/2 males result in pregnancies with a high rate of failure/resorption, while other crosses with CBA/J females produce normal pregnancies. Although CBA/J x DBA/2 has frequently been used as a model for miscarriage, a detailed explanation for the pregnancy failure phenotype is lacking. We performed timed matings between CBA/J female and DBA/2 male mice as well as the reverse mating, DBA/2 female with CBA/J males. Decidual caps were isolated at E9.5 from both crosses and a microarray-based method was used to comparatively assess genomic methylation at approximately 16,000 loci on mouse chromosome 7. In comparison with decidual caps from DBA/2 x CBA/J pregnancies, CBA/J x DBA/2 decidual caps were characterized by widely and apparently randomly disturbed methylation. In another set of analogous experiments, genomic methylation of placental DNA from E8.5 pregnancies was assessed using the same microarraybased method. This analysis revealed that, in contrast to the decidua, placental DNA methylation from CBA/J x DBA/2 pregnancies was indistinguishable from normal controls. We conclude that abnormal DNA methylation in the uterine decidua is likely to play a role in the CBA/J x DBA/2 model of pregnancy failure. Our studies are the first to demonstrate that epigenetic abnormalities of the decidua are associated with pregnancy failure and set the stage for future efforts aimed at understanding the role of DNA methylation at the maternal-fetal interface.
Tanjina Kader - One of the best experts on this subject based on the ideXlab platform.
-
copy number analysis by low coverage whole genome sequencing using ultra low input dna from formalin fixed paraffin embedded tumor tissue
2016Co-Authors: Tanjina Kader, David L Goode, Stephen Q Wong, Jacquie Connaughton, Simone M Rowley, Lisa Devereux, David J Byrne, Stephen B FoxAbstract:Unlocking clinically translatable genomic information, including copy number alterations (CNA), from formalin-fixed paraffin-embedded (FFPE) tissue is challenging due to low yields and degraded DNA. We describe a robust, cost-effective low-coverage whole genome sequencing (LC WGS) method for CNA detection using 5 ng of FFPE-derived DNA. CN profiles using 100 ng or 5 ng input DNA were highly concordant and comparable with molecular inversion probe (MIP) array profiles. LC WGS improved CN profiles of samples that performed poorly using MIP arrays. Our technique enables identification of driver and prognostic CNAs in archival patient samples previously deemed unsuitable for genomic analysis due to DNA limitations.
