The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform
Alain Chanson - One of the best experts on this subject based on the ideXlab platform.
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Subcellular localization, distribution and expression of calmodulin in Zea Mays roots
Plant Science, 1996Co-Authors: A. Fraichard, E. Perotti, Olivier Gavin, Alain ChansonAbstract:The subcellular localization, distribution and the steady state level of calmodulin from maize roots (Zea Mays L., cv. LG 11) were studied. To analyze the subcellular localization, 2-day old root membranes were fractionated by sucrose density gradient centrifugation and immunoblotting was done with an antibody raised against a vertebrate calmodulin (SWant) which recognized the plant calmodulin. Calmodulin was principally associated with high density fractions and particularly plasmalemma. For studying the distribution of calmodulin in various zones of Zea Mays roots, a micro method of membrane preparation was developed. Most of the calmodulin was present in microsomes isolated from the root apex corresponding to the first 4 mm of a 15 ± 2 mm root. An identical distribution was found by studying the steady state level of the protein by Northern blotting using a cDNA clone of Zea Mays calmodulin.
Raúl Arredondo-peter - One of the best experts on this subject based on the ideXlab platform.
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Cloning and expression analysis of hemoglobin genes from maize (Zea Mays ssp. Mays) and teosinte (Zea Mays ssp. parviglumis).
Biochimica et biophysica acta, 2001Co-Authors: Elena Aréchaga-ocampo, Juan Saenz-rivera, Gautam Sarath, Robert V. Klucas, Raúl Arredondo-peterAbstract:With the exception of barley and rice, little is known about the existence of hemoglobins (Hbs) in cereals. This work reports the cloning and analysis of hb genes from maize (Zea Mays ssp. Mays) and teosinte (Zea Mays ssp. parviglumis). Coding sequences of maize and teosinte hb genes (hbm and hbt, respectively) are highly similar to each other and are interrupted by three introns located at identical positions as other plant hb genes. Sequences of predicted Hbm and Hbt proteins are identical. The hydropathic profile of Hbm and Hbt is highly similar to that of rice Hb1, suggesting that Hbm, Hbt and Hb1 have the same tertiary structure and biochemical properties. Expression analysis showed that low levels of Hb transcripts, but considerable levels of Hb proteins exist in maize embryonic organs. No Hb transcripts and proteins were detected in teosinte embryonic organs. Low levels of Hb proteins, but no Hb transcripts, were detected in maize and teosinte vegetative organs. These observations suggest that the regulation of hb genes is different in maize and teosinte embryonic organs, and that the expression of hb genes is down- or up-regulated in maize and teosinte, respectively, from germination to vegetative growing.
A. Fraichard - One of the best experts on this subject based on the ideXlab platform.
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Subcellular localization, distribution and expression of calmodulin in Zea Mays roots
Plant Science, 1996Co-Authors: A. Fraichard, E. Perotti, Olivier Gavin, Alain ChansonAbstract:The subcellular localization, distribution and the steady state level of calmodulin from maize roots (Zea Mays L., cv. LG 11) were studied. To analyze the subcellular localization, 2-day old root membranes were fractionated by sucrose density gradient centrifugation and immunoblotting was done with an antibody raised against a vertebrate calmodulin (SWant) which recognized the plant calmodulin. Calmodulin was principally associated with high density fractions and particularly plasmalemma. For studying the distribution of calmodulin in various zones of Zea Mays roots, a micro method of membrane preparation was developed. Most of the calmodulin was present in microsomes isolated from the root apex corresponding to the first 4 mm of a 15 ± 2 mm root. An identical distribution was found by studying the steady state level of the protein by Northern blotting using a cDNA clone of Zea Mays calmodulin.
S. L. Neoh - One of the best experts on this subject based on the ideXlab platform.
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Frying quality of virgin coconut oil as affected by Zea Mays extract
2014Co-Authors: A.m. Marina, W. I. Wan Rosli, S. L. NeohAbstract:The effect of corn silk or Zea Mays extract on the physicochemical changes of virgin coconut oil was studied during three consecutive days of deep frying. There were three types of oil blend systems used: virgin coconut oil without antioxidant as control system; virgin coconut oil with butylated hydroxytoluene (BHT) as synthetic antioxidant and virgin coconut oil with Zea Mays extract as natural antioxidant. The oil quality was assessed by measuring the colour, viscosity, peroxide, p-anisidine, free fatty acids, totox and iodine value. The results show that Zea Mays extract delayed the oil deterioration. The Zea Mays extract significantly (p
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Frying Quality of Virgin Coconut Oil as Affected by Zea Mays Extract (Kualiti Menggoreng Minyak Kelapa Dara Disebabkan oleh Ekstrak Zea Mays)
2014Co-Authors: A.m. Marina, W. I. Wan Rosli, S. L. NeohAbstract:The effect of corn silk or Zea Mays extract on the physicochemical changes of virgin coconut oil was studied during three consecutive days of deep frying. There were three types of oil blend systems used: virgin coconut oil without antioxidant as control system; virgin coconut oil with butylated hydroxytoluene (BHT) as synthetic antioxidant and virgin coconut oil with Zea Mays extract as natural antioxidant. The oil quality was assessed by measuring the colour, viscosity, peroxide, p-anisidine, free fatty acids, totox and iodine value. The results show that Zea Mays extract delayed the oil deterioration. The Zea Mays extract significantly ( p
Elena Aréchaga-ocampo - One of the best experts on this subject based on the ideXlab platform.
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Cloning and expression analysis of hemoglobin genes from maize (Zea Mays ssp. Mays) and teosinte (Zea Mays ssp. parviglumis).
Biochimica et biophysica acta, 2001Co-Authors: Elena Aréchaga-ocampo, Juan Saenz-rivera, Gautam Sarath, Robert V. Klucas, Raúl Arredondo-peterAbstract:With the exception of barley and rice, little is known about the existence of hemoglobins (Hbs) in cereals. This work reports the cloning and analysis of hb genes from maize (Zea Mays ssp. Mays) and teosinte (Zea Mays ssp. parviglumis). Coding sequences of maize and teosinte hb genes (hbm and hbt, respectively) are highly similar to each other and are interrupted by three introns located at identical positions as other plant hb genes. Sequences of predicted Hbm and Hbt proteins are identical. The hydropathic profile of Hbm and Hbt is highly similar to that of rice Hb1, suggesting that Hbm, Hbt and Hb1 have the same tertiary structure and biochemical properties. Expression analysis showed that low levels of Hb transcripts, but considerable levels of Hb proteins exist in maize embryonic organs. No Hb transcripts and proteins were detected in teosinte embryonic organs. Low levels of Hb proteins, but no Hb transcripts, were detected in maize and teosinte vegetative organs. These observations suggest that the regulation of hb genes is different in maize and teosinte embryonic organs, and that the expression of hb genes is down- or up-regulated in maize and teosinte, respectively, from germination to vegetative growing.
