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Evanthia Karamitopoulou - One of the best experts on this subject based on the ideXlab platform.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:Results: Tumour-budding cells showed increased levels of ZEB1 (Po0.0001) and ZEB2 (P ¼0.0119) and reduced E-cadherin and b-catenin (Po0.0001, each) compared with the main tumour. Loss of membranous b-catenin in the main tumour (P ¼0.0009) and tumour buds (P ¼0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P ¼0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P ¼0.0084; 0.0250 and 0.0029, respectively) and high PTB (P ¼0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P ¼0.03), lymphatic invasion (P ¼0.0172) and lymph node metastasis (P ¼0.0152). Conclusions: In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:There is evidence that tumour–stroma interactions have a major role in the neoplastic progression of pancreatic ductal adenocarcinoma (PDAC). Tumour budding is thought to reflect the process of epithelial–mesenchymal transition (EMT); however, the relationship between tumour buds and EMT remains unclear. Here we characterize the tumour-budding- and stromal cells in PDAC at protein and mRNA levels concerning factors involved in EMT. mRNA in situ hybridisation and immunostaining for E-cadherin, β-catenin, SNAIL1, ZEB1, ZEB2, N-cadherin and TWIST1 were assessed in the main tumour, tumour buds and tumour stroma on multipunch tissue microarrays from 120 well-characterised PDACs and associated with the clinicopathological features, including peritumoural (PTB) and intratumoural (ITB) budding. Tumour-budding cells showed increased levels of ZEB1 (P<0.0001) and ZEB2 (P=0.0119) and reduced E-cadherin and β-catenin (P<0.0001, each) compared with the main tumour. Loss of membranous β-catenin in the main tumour (P=0.0009) and tumour buds (P=0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P=0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P=0.0084; 0.0250 and 0.0029, respectively) and high PTB (P=0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P=0.03), lymphatic invasion (P=0.0172) and lymph node metastasis (P=0.0152). In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
Jose A Galvan - One of the best experts on this subject based on the ideXlab platform.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:Results: Tumour-budding cells showed increased levels of ZEB1 (Po0.0001) and ZEB2 (P ¼0.0119) and reduced E-cadherin and b-catenin (Po0.0001, each) compared with the main tumour. Loss of membranous b-catenin in the main tumour (P ¼0.0009) and tumour buds (P ¼0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P ¼0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P ¼0.0084; 0.0250 and 0.0029, respectively) and high PTB (P ¼0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P ¼0.03), lymphatic invasion (P ¼0.0172) and lymph node metastasis (P ¼0.0152). Conclusions: In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:There is evidence that tumour–stroma interactions have a major role in the neoplastic progression of pancreatic ductal adenocarcinoma (PDAC). Tumour budding is thought to reflect the process of epithelial–mesenchymal transition (EMT); however, the relationship between tumour buds and EMT remains unclear. Here we characterize the tumour-budding- and stromal cells in PDAC at protein and mRNA levels concerning factors involved in EMT. mRNA in situ hybridisation and immunostaining for E-cadherin, β-catenin, SNAIL1, ZEB1, ZEB2, N-cadherin and TWIST1 were assessed in the main tumour, tumour buds and tumour stroma on multipunch tissue microarrays from 120 well-characterised PDACs and associated with the clinicopathological features, including peritumoural (PTB) and intratumoural (ITB) budding. Tumour-budding cells showed increased levels of ZEB1 (P<0.0001) and ZEB2 (P=0.0119) and reduced E-cadherin and β-catenin (P<0.0001, each) compared with the main tumour. Loss of membranous β-catenin in the main tumour (P=0.0009) and tumour buds (P=0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P=0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P=0.0084; 0.0250 and 0.0029, respectively) and high PTB (P=0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P=0.03), lymphatic invasion (P=0.0172) and lymph node metastasis (P=0.0152). In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
Inti Zlobec - One of the best experts on this subject based on the ideXlab platform.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:Results: Tumour-budding cells showed increased levels of ZEB1 (Po0.0001) and ZEB2 (P ¼0.0119) and reduced E-cadherin and b-catenin (Po0.0001, each) compared with the main tumour. Loss of membranous b-catenin in the main tumour (P ¼0.0009) and tumour buds (P ¼0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P ¼0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P ¼0.0084; 0.0250 and 0.0029, respectively) and high PTB (P ¼0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P ¼0.03), lymphatic invasion (P ¼0.0172) and lymph node metastasis (P ¼0.0152). Conclusions: In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:There is evidence that tumour–stroma interactions have a major role in the neoplastic progression of pancreatic ductal adenocarcinoma (PDAC). Tumour budding is thought to reflect the process of epithelial–mesenchymal transition (EMT); however, the relationship between tumour buds and EMT remains unclear. Here we characterize the tumour-budding- and stromal cells in PDAC at protein and mRNA levels concerning factors involved in EMT. mRNA in situ hybridisation and immunostaining for E-cadherin, β-catenin, SNAIL1, ZEB1, ZEB2, N-cadherin and TWIST1 were assessed in the main tumour, tumour buds and tumour stroma on multipunch tissue microarrays from 120 well-characterised PDACs and associated with the clinicopathological features, including peritumoural (PTB) and intratumoural (ITB) budding. Tumour-budding cells showed increased levels of ZEB1 (P<0.0001) and ZEB2 (P=0.0119) and reduced E-cadherin and β-catenin (P<0.0001, each) compared with the main tumour. Loss of membranous β-catenin in the main tumour (P=0.0009) and tumour buds (P=0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P=0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P=0.0084; 0.0250 and 0.0029, respectively) and high PTB (P=0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P=0.03), lymphatic invasion (P=0.0172) and lymph node metastasis (P=0.0152). In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
Martin Wartenberg - One of the best experts on this subject based on the ideXlab platform.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:Results: Tumour-budding cells showed increased levels of ZEB1 (Po0.0001) and ZEB2 (P ¼0.0119) and reduced E-cadherin and b-catenin (Po0.0001, each) compared with the main tumour. Loss of membranous b-catenin in the main tumour (P ¼0.0009) and tumour buds (P ¼0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P ¼0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P ¼0.0084; 0.0250 and 0.0029, respectively) and high PTB (P ¼0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P ¼0.03), lymphatic invasion (P ¼0.0172) and lymph node metastasis (P ¼0.0152). Conclusions: In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:There is evidence that tumour–stroma interactions have a major role in the neoplastic progression of pancreatic ductal adenocarcinoma (PDAC). Tumour budding is thought to reflect the process of epithelial–mesenchymal transition (EMT); however, the relationship between tumour buds and EMT remains unclear. Here we characterize the tumour-budding- and stromal cells in PDAC at protein and mRNA levels concerning factors involved in EMT. mRNA in situ hybridisation and immunostaining for E-cadherin, β-catenin, SNAIL1, ZEB1, ZEB2, N-cadherin and TWIST1 were assessed in the main tumour, tumour buds and tumour stroma on multipunch tissue microarrays from 120 well-characterised PDACs and associated with the clinicopathological features, including peritumoural (PTB) and intratumoural (ITB) budding. Tumour-budding cells showed increased levels of ZEB1 (P<0.0001) and ZEB2 (P=0.0119) and reduced E-cadherin and β-catenin (P<0.0001, each) compared with the main tumour. Loss of membranous β-catenin in the main tumour (P=0.0009) and tumour buds (P=0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P=0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P=0.0084; 0.0250 and 0.0029, respectively) and high PTB (P=0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P=0.03), lymphatic invasion (P=0.0172) and lymph node metastasis (P=0.0152). In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
Alessandro Lugli - One of the best experts on this subject based on the ideXlab platform.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:Results: Tumour-budding cells showed increased levels of ZEB1 (Po0.0001) and ZEB2 (P ¼0.0119) and reduced E-cadherin and b-catenin (Po0.0001, each) compared with the main tumour. Loss of membranous b-catenin in the main tumour (P ¼0.0009) and tumour buds (P ¼0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P ¼0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P ¼0.0084; 0.0250 and 0.0029, respectively) and high PTB (P ¼0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P ¼0.03), lymphatic invasion (P ¼0.0172) and lymph node metastasis (P ¼0.0152). Conclusions: In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
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expression of e cadherin repressors snail ZEB1 and zeb2 by tumour and stromal cells influences tumour budding phenotype and suggests heterogeneity of stromal cells in pancreatic cancer
British Journal of Cancer, 2015Co-Authors: Jose A Galvan, Inti Zlobec, Martin Wartenberg, Alessandro Lugli, Beat Gloor, Aurel Perren, Evanthia KaramitopoulouAbstract:There is evidence that tumour–stroma interactions have a major role in the neoplastic progression of pancreatic ductal adenocarcinoma (PDAC). Tumour budding is thought to reflect the process of epithelial–mesenchymal transition (EMT); however, the relationship between tumour buds and EMT remains unclear. Here we characterize the tumour-budding- and stromal cells in PDAC at protein and mRNA levels concerning factors involved in EMT. mRNA in situ hybridisation and immunostaining for E-cadherin, β-catenin, SNAIL1, ZEB1, ZEB2, N-cadherin and TWIST1 were assessed in the main tumour, tumour buds and tumour stroma on multipunch tissue microarrays from 120 well-characterised PDACs and associated with the clinicopathological features, including peritumoural (PTB) and intratumoural (ITB) budding. Tumour-budding cells showed increased levels of ZEB1 (P<0.0001) and ZEB2 (P=0.0119) and reduced E-cadherin and β-catenin (P<0.0001, each) compared with the main tumour. Loss of membranous β-catenin in the main tumour (P=0.0009) and tumour buds (P=0.0053), without nuclear translocation, as well as increased SNAIL1 in tumour and stromal cells (P=0.0002, each) correlated with high PTB. ZEB1 overexpression in the main tumour-budding and stromal cells was associated with high ITB (P=0.0084; 0.0250 and 0.0029, respectively) and high PTB (P=0.0005; 0.0392 and 0.0007, respectively). ZEB2 overexpression in stromal cells correlated with higher pT stage (P=0.03), lymphatic invasion (P=0.0172) and lymph node metastasis (P=0.0152). In the tumour microenvironment of phenotypically aggressive PDAC, tumour-budding cells express EMT hallmarks at protein and mRNA levels underlining their EMT-type character and are surrounded by stromal cells expressing high levels of the E-cadherin repressors ZEB1, ZEB2 and SNAIL1, this being strongly associated with the tumour-budding phenotype. Moreover, our findings suggest the existence of subtypes of stromal cells in PDAC with phenotypical and functional heterogeneity.
