The Experts below are selected from a list of 5166 Experts worldwide ranked by ideXlab platform
Wen Dai - One of the best experts on this subject based on the ideXlab platform.
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diagnostic accuracy of intracellular mycobacterium tuberculosis detection for tuberculous meningitis
American Journal of Respiratory and Critical Care Medicine, 2014Co-Authors: Guodong Feng, Ming Shi, Ping Chen, Bingju Wang, Min Zhang, Xiaolin Chang, Yining Yang, Xinhong Fan, Wen Dai, Tingting LiuAbstract:Rationale: Early diagnosis and treatment of tuberculous meningitis saves lives, but current laboratory diagnostic tests lack sensitivity.Objectives: We investigated whether the detection of intracellular bacteria by a modified Ziehl-Neelsen Stain and early secretory antigen target (ESAT)-6 in cerebrospinal fluid leukocytes improves tuberculous meningitis diagnosis.Methods: Cerebrospinal fluid specimens from patients with suspected tuberculous meningitis were Stained by conventional Ziehl-Neelsen Stain, a modified Ziehl-Neelsen Stain involving cytospin slides with Triton processing, and an ESAT-6 immunocytochemical Stain. Acid-fast bacteria and ESAT-6–expressing leukocytes were detected by microscopy. All tests were performed prospectively in a central laboratory by experienced technicians masked to the patients’ final diagnosis.Measurements and Main Results: Two hundred and eighty patients with suspected tuberculous meningitis were enrolled. Thirty-seven had Mycobacterium tuberculosis cultured from cerebr...
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A Highly Efficient Ziehl-Neelsen Stain: Identifying De Novo Intracellular Mycobacterium tuberculosis and Improving Detection of Extracellular M. tuberculosis in Cerebrospinal Fluid
Journal of clinical microbiology, 2012Co-Authors: Ping Chen, Guodong Feng, Ming Shi, Bingju Wang, Yining Yang, Jia-yun Liu, Xiaodan Shi, Xuedong Liu, Wen DaiAbstract:ABSTRACT Tuberculous meningitis leads to a devastating outcome, and early diagnosis and rapid chemotherapy are vital to reduce morbidity and mortality. Since Mycobacterium tuberculosis is a kind of cytozoic pathogen and its numbers are very few in cerebrospinal fluid, detecting M. tuberculosis in cerebrospinal fluid from tuberculous meningitis patients is still a challenge for clinicians. Ziehl-Neelsen Stain, the current feasible microbiological method for the diagnosis of tuberculosis, often needs a large amount of cerebrospinal fluid specimen but shows a low detection rate of M. tuberculosis. Here, we developed a modified Ziehl-Neelsen Stain, involving cytospin slides with Triton processing, in which only 0.5 ml of cerebrospinal fluid specimens was required. This method not only improved the detection rate of extracellular M. tuberculosis significantly but also identified intracellular M. tuberculosis in the neutrophils, monocytes, and lymphocytes clearly. Thus, our modified method is more effective and sensitive than the conventional Ziehl-Neelsen Stain, providing clinicians a convenient yet powerful tool for rapidly diagnosing tuberculous meningitis.
Guodong Feng - One of the best experts on this subject based on the ideXlab platform.
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diagnostic accuracy of intracellular mycobacterium tuberculosis detection for tuberculous meningitis
American Journal of Respiratory and Critical Care Medicine, 2014Co-Authors: Guodong Feng, Ming Shi, Ping Chen, Bingju Wang, Min Zhang, Xiaolin Chang, Yining Yang, Xinhong Fan, Wen Dai, Tingting LiuAbstract:Rationale: Early diagnosis and treatment of tuberculous meningitis saves lives, but current laboratory diagnostic tests lack sensitivity.Objectives: We investigated whether the detection of intracellular bacteria by a modified Ziehl-Neelsen Stain and early secretory antigen target (ESAT)-6 in cerebrospinal fluid leukocytes improves tuberculous meningitis diagnosis.Methods: Cerebrospinal fluid specimens from patients with suspected tuberculous meningitis were Stained by conventional Ziehl-Neelsen Stain, a modified Ziehl-Neelsen Stain involving cytospin slides with Triton processing, and an ESAT-6 immunocytochemical Stain. Acid-fast bacteria and ESAT-6–expressing leukocytes were detected by microscopy. All tests were performed prospectively in a central laboratory by experienced technicians masked to the patients’ final diagnosis.Measurements and Main Results: Two hundred and eighty patients with suspected tuberculous meningitis were enrolled. Thirty-seven had Mycobacterium tuberculosis cultured from cerebr...
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A Highly Efficient Ziehl-Neelsen Stain: Identifying De Novo Intracellular Mycobacterium tuberculosis and Improving Detection of Extracellular M. tuberculosis in Cerebrospinal Fluid
Journal of clinical microbiology, 2012Co-Authors: Ping Chen, Guodong Feng, Ming Shi, Bingju Wang, Yining Yang, Jia-yun Liu, Xiaodan Shi, Xuedong Liu, Wen DaiAbstract:ABSTRACT Tuberculous meningitis leads to a devastating outcome, and early diagnosis and rapid chemotherapy are vital to reduce morbidity and mortality. Since Mycobacterium tuberculosis is a kind of cytozoic pathogen and its numbers are very few in cerebrospinal fluid, detecting M. tuberculosis in cerebrospinal fluid from tuberculous meningitis patients is still a challenge for clinicians. Ziehl-Neelsen Stain, the current feasible microbiological method for the diagnosis of tuberculosis, often needs a large amount of cerebrospinal fluid specimen but shows a low detection rate of M. tuberculosis. Here, we developed a modified Ziehl-Neelsen Stain, involving cytospin slides with Triton processing, in which only 0.5 ml of cerebrospinal fluid specimens was required. This method not only improved the detection rate of extracellular M. tuberculosis significantly but also identified intracellular M. tuberculosis in the neutrophils, monocytes, and lymphocytes clearly. Thus, our modified method is more effective and sensitive than the conventional Ziehl-Neelsen Stain, providing clinicians a convenient yet powerful tool for rapidly diagnosing tuberculous meningitis.
Ping Chen - One of the best experts on this subject based on the ideXlab platform.
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diagnostic accuracy of intracellular mycobacterium tuberculosis detection for tuberculous meningitis
American Journal of Respiratory and Critical Care Medicine, 2014Co-Authors: Guodong Feng, Ming Shi, Ping Chen, Bingju Wang, Min Zhang, Xiaolin Chang, Yining Yang, Xinhong Fan, Wen Dai, Tingting LiuAbstract:Rationale: Early diagnosis and treatment of tuberculous meningitis saves lives, but current laboratory diagnostic tests lack sensitivity.Objectives: We investigated whether the detection of intracellular bacteria by a modified Ziehl-Neelsen Stain and early secretory antigen target (ESAT)-6 in cerebrospinal fluid leukocytes improves tuberculous meningitis diagnosis.Methods: Cerebrospinal fluid specimens from patients with suspected tuberculous meningitis were Stained by conventional Ziehl-Neelsen Stain, a modified Ziehl-Neelsen Stain involving cytospin slides with Triton processing, and an ESAT-6 immunocytochemical Stain. Acid-fast bacteria and ESAT-6–expressing leukocytes were detected by microscopy. All tests were performed prospectively in a central laboratory by experienced technicians masked to the patients’ final diagnosis.Measurements and Main Results: Two hundred and eighty patients with suspected tuberculous meningitis were enrolled. Thirty-seven had Mycobacterium tuberculosis cultured from cerebr...
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A Highly Efficient Ziehl-Neelsen Stain: Identifying De Novo Intracellular Mycobacterium tuberculosis and Improving Detection of Extracellular M. tuberculosis in Cerebrospinal Fluid
Journal of clinical microbiology, 2012Co-Authors: Ping Chen, Guodong Feng, Ming Shi, Bingju Wang, Yining Yang, Jia-yun Liu, Xiaodan Shi, Xuedong Liu, Wen DaiAbstract:ABSTRACT Tuberculous meningitis leads to a devastating outcome, and early diagnosis and rapid chemotherapy are vital to reduce morbidity and mortality. Since Mycobacterium tuberculosis is a kind of cytozoic pathogen and its numbers are very few in cerebrospinal fluid, detecting M. tuberculosis in cerebrospinal fluid from tuberculous meningitis patients is still a challenge for clinicians. Ziehl-Neelsen Stain, the current feasible microbiological method for the diagnosis of tuberculosis, often needs a large amount of cerebrospinal fluid specimen but shows a low detection rate of M. tuberculosis. Here, we developed a modified Ziehl-Neelsen Stain, involving cytospin slides with Triton processing, in which only 0.5 ml of cerebrospinal fluid specimens was required. This method not only improved the detection rate of extracellular M. tuberculosis significantly but also identified intracellular M. tuberculosis in the neutrophils, monocytes, and lymphocytes clearly. Thus, our modified method is more effective and sensitive than the conventional Ziehl-Neelsen Stain, providing clinicians a convenient yet powerful tool for rapidly diagnosing tuberculous meningitis.
Ming Shi - One of the best experts on this subject based on the ideXlab platform.
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diagnostic accuracy of intracellular mycobacterium tuberculosis detection for tuberculous meningitis
American Journal of Respiratory and Critical Care Medicine, 2014Co-Authors: Guodong Feng, Ming Shi, Ping Chen, Bingju Wang, Min Zhang, Xiaolin Chang, Yining Yang, Xinhong Fan, Wen Dai, Tingting LiuAbstract:Rationale: Early diagnosis and treatment of tuberculous meningitis saves lives, but current laboratory diagnostic tests lack sensitivity.Objectives: We investigated whether the detection of intracellular bacteria by a modified Ziehl-Neelsen Stain and early secretory antigen target (ESAT)-6 in cerebrospinal fluid leukocytes improves tuberculous meningitis diagnosis.Methods: Cerebrospinal fluid specimens from patients with suspected tuberculous meningitis were Stained by conventional Ziehl-Neelsen Stain, a modified Ziehl-Neelsen Stain involving cytospin slides with Triton processing, and an ESAT-6 immunocytochemical Stain. Acid-fast bacteria and ESAT-6–expressing leukocytes were detected by microscopy. All tests were performed prospectively in a central laboratory by experienced technicians masked to the patients’ final diagnosis.Measurements and Main Results: Two hundred and eighty patients with suspected tuberculous meningitis were enrolled. Thirty-seven had Mycobacterium tuberculosis cultured from cerebr...
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A Highly Efficient Ziehl-Neelsen Stain: Identifying De Novo Intracellular Mycobacterium tuberculosis and Improving Detection of Extracellular M. tuberculosis in Cerebrospinal Fluid
Journal of clinical microbiology, 2012Co-Authors: Ping Chen, Guodong Feng, Ming Shi, Bingju Wang, Yining Yang, Jia-yun Liu, Xiaodan Shi, Xuedong Liu, Wen DaiAbstract:ABSTRACT Tuberculous meningitis leads to a devastating outcome, and early diagnosis and rapid chemotherapy are vital to reduce morbidity and mortality. Since Mycobacterium tuberculosis is a kind of cytozoic pathogen and its numbers are very few in cerebrospinal fluid, detecting M. tuberculosis in cerebrospinal fluid from tuberculous meningitis patients is still a challenge for clinicians. Ziehl-Neelsen Stain, the current feasible microbiological method for the diagnosis of tuberculosis, often needs a large amount of cerebrospinal fluid specimen but shows a low detection rate of M. tuberculosis. Here, we developed a modified Ziehl-Neelsen Stain, involving cytospin slides with Triton processing, in which only 0.5 ml of cerebrospinal fluid specimens was required. This method not only improved the detection rate of extracellular M. tuberculosis significantly but also identified intracellular M. tuberculosis in the neutrophils, monocytes, and lymphocytes clearly. Thus, our modified method is more effective and sensitive than the conventional Ziehl-Neelsen Stain, providing clinicians a convenient yet powerful tool for rapidly diagnosing tuberculous meningitis.
Yining Yang - One of the best experts on this subject based on the ideXlab platform.
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diagnostic accuracy of intracellular mycobacterium tuberculosis detection for tuberculous meningitis
American Journal of Respiratory and Critical Care Medicine, 2014Co-Authors: Guodong Feng, Ming Shi, Ping Chen, Bingju Wang, Min Zhang, Xiaolin Chang, Yining Yang, Xinhong Fan, Wen Dai, Tingting LiuAbstract:Rationale: Early diagnosis and treatment of tuberculous meningitis saves lives, but current laboratory diagnostic tests lack sensitivity.Objectives: We investigated whether the detection of intracellular bacteria by a modified Ziehl-Neelsen Stain and early secretory antigen target (ESAT)-6 in cerebrospinal fluid leukocytes improves tuberculous meningitis diagnosis.Methods: Cerebrospinal fluid specimens from patients with suspected tuberculous meningitis were Stained by conventional Ziehl-Neelsen Stain, a modified Ziehl-Neelsen Stain involving cytospin slides with Triton processing, and an ESAT-6 immunocytochemical Stain. Acid-fast bacteria and ESAT-6–expressing leukocytes were detected by microscopy. All tests were performed prospectively in a central laboratory by experienced technicians masked to the patients’ final diagnosis.Measurements and Main Results: Two hundred and eighty patients with suspected tuberculous meningitis were enrolled. Thirty-seven had Mycobacterium tuberculosis cultured from cerebr...
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A Highly Efficient Ziehl-Neelsen Stain: Identifying De Novo Intracellular Mycobacterium tuberculosis and Improving Detection of Extracellular M. tuberculosis in Cerebrospinal Fluid
Journal of clinical microbiology, 2012Co-Authors: Ping Chen, Guodong Feng, Ming Shi, Bingju Wang, Yining Yang, Jia-yun Liu, Xiaodan Shi, Xuedong Liu, Wen DaiAbstract:ABSTRACT Tuberculous meningitis leads to a devastating outcome, and early diagnosis and rapid chemotherapy are vital to reduce morbidity and mortality. Since Mycobacterium tuberculosis is a kind of cytozoic pathogen and its numbers are very few in cerebrospinal fluid, detecting M. tuberculosis in cerebrospinal fluid from tuberculous meningitis patients is still a challenge for clinicians. Ziehl-Neelsen Stain, the current feasible microbiological method for the diagnosis of tuberculosis, often needs a large amount of cerebrospinal fluid specimen but shows a low detection rate of M. tuberculosis. Here, we developed a modified Ziehl-Neelsen Stain, involving cytospin slides with Triton processing, in which only 0.5 ml of cerebrospinal fluid specimens was required. This method not only improved the detection rate of extracellular M. tuberculosis significantly but also identified intracellular M. tuberculosis in the neutrophils, monocytes, and lymphocytes clearly. Thus, our modified method is more effective and sensitive than the conventional Ziehl-Neelsen Stain, providing clinicians a convenient yet powerful tool for rapidly diagnosing tuberculous meningitis.
