The Experts below are selected from a list of 3 Experts worldwide ranked by ideXlab platform
Tsutomu Fukuwatari - One of the best experts on this subject based on the ideXlab platform.
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High-performance liquid chromatographic method for profiling 2-oxo Acids in urine and its application in evaluating vitamin status in rats
2015Co-Authors: Katsumi Shibata, Chifumi Nakata, Tsutomu FukuwatariAbstract:B-group vitamins are involved in the catabolism of 2-oxo Acids. To identify the functional biomarkers of B-group vitamins, we developed a high-performance liquid chromatographic method for profiling 2-oxo Acids in urine and applied this method to urine samples from rats deficient in vitamins B1 and B6 and pantothenic Acid. 2-Oxo Acids were reacted with 1,2-diamino-4,5-methylenebenzene to produce fluorescent derivatives, which were then separated using a TSKgel ODS-80Ts column with 30 mmol/L of KH2PO4 (pH 3.0):acetonitrile (7:3) at a flow rate of 1.0 mL/min. Vitamin B1 deficiency increased urinary levels of all 2-oxo Acids, while vitamin B6 deficiency only increased levels of sum of 2-oxaloacetic Acid and pyruvic Acid, and pantothenic Acid deficiency only increased levels of 2-Oxoisovaleric Acid. Profiles of 2-oxo Acids in urine samples might be a non-invasive way of clarifying the functional biomarker of B-group vitamins.
Katsumi Shibata - One of the best experts on this subject based on the ideXlab platform.
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High-performance liquid chromatographic method for profiling 2-oxo Acids in urine and its application in evaluating vitamin status in rats
2015Co-Authors: Katsumi Shibata, Chifumi Nakata, Tsutomu FukuwatariAbstract:B-group vitamins are involved in the catabolism of 2-oxo Acids. To identify the functional biomarkers of B-group vitamins, we developed a high-performance liquid chromatographic method for profiling 2-oxo Acids in urine and applied this method to urine samples from rats deficient in vitamins B1 and B6 and pantothenic Acid. 2-Oxo Acids were reacted with 1,2-diamino-4,5-methylenebenzene to produce fluorescent derivatives, which were then separated using a TSKgel ODS-80Ts column with 30 mmol/L of KH2PO4 (pH 3.0):acetonitrile (7:3) at a flow rate of 1.0 mL/min. Vitamin B1 deficiency increased urinary levels of all 2-oxo Acids, while vitamin B6 deficiency only increased levels of sum of 2-oxaloacetic Acid and pyruvic Acid, and pantothenic Acid deficiency only increased levels of 2-Oxoisovaleric Acid. Profiles of 2-oxo Acids in urine samples might be a non-invasive way of clarifying the functional biomarker of B-group vitamins.
Chifumi Nakata - One of the best experts on this subject based on the ideXlab platform.
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High-performance liquid chromatographic method for profiling 2-oxo Acids in urine and its application in evaluating vitamin status in rats
2015Co-Authors: Katsumi Shibata, Chifumi Nakata, Tsutomu FukuwatariAbstract:B-group vitamins are involved in the catabolism of 2-oxo Acids. To identify the functional biomarkers of B-group vitamins, we developed a high-performance liquid chromatographic method for profiling 2-oxo Acids in urine and applied this method to urine samples from rats deficient in vitamins B1 and B6 and pantothenic Acid. 2-Oxo Acids were reacted with 1,2-diamino-4,5-methylenebenzene to produce fluorescent derivatives, which were then separated using a TSKgel ODS-80Ts column with 30 mmol/L of KH2PO4 (pH 3.0):acetonitrile (7:3) at a flow rate of 1.0 mL/min. Vitamin B1 deficiency increased urinary levels of all 2-oxo Acids, while vitamin B6 deficiency only increased levels of sum of 2-oxaloacetic Acid and pyruvic Acid, and pantothenic Acid deficiency only increased levels of 2-Oxoisovaleric Acid. Profiles of 2-oxo Acids in urine samples might be a non-invasive way of clarifying the functional biomarker of B-group vitamins.
