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Jean D Wilson - One of the best experts on this subject based on the ideXlab platform.
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formation of 5α reduced androgens in the testes and urogenital tract of the grey short tailed opossum monodelphis domestica
Reproduction Fertility and Development, 2009Co-Authors: Jean D Wilson, Marilyn B Renfree, Richard J Auchus, Andrew J Pask, Geoffrey ShawAbstract:Testicular 5α-reduced androgens, largely 5α-androstane-3α,17β-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with ( 3 H)-progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). The only 19-carbon metabolites identified in the youngest ages (5-26 days) and the major metabolites in adult testes were testosterone and androstenedione. At 30, 42 and 49 days of age, dihydrotestosterone and small amounts of androstanediol were present. Time-sequence studies indicated that dihydrotestosterone and androstanediol were formed from the 5α-reduction (and 3-keto reduction) of testosterone. In a second series of experiments, tissue fragments of a variety of urogenital tract tissues were incubated with ( 3 H)-testosterone and the metabolites separated by HPLC. During the interval in which male urogenital tract differentiation takes place in this species (between Days 15 and 28), the major metabolite identified was dihydrotestosterone. We conclude that the timing of 5α-reductase expression in the testes of the grey short-tailed possum resembles that of rodents and the brushtail possum rather than that of the tammar wallaby and that dihydrotestosterone is probably the intracellular androgen responsible for virilisation of the urogenital tract in this species.
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formation of 5α reduced androgens in the testes and urogenital tract of the grey short tailed opossum monodelphis domestica
Reproduction Fertility and Development, 2009Co-Authors: Jean D Wilson, Marilyn B Renfree, Richard J Auchus, Andrew J Pask, Geoffrey ShawAbstract:Testicular 5alpha-reduced androgens, largely 5alpha-androstane-3alpha,17beta-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with [3H]-progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). The only 19-carbon metabolites identified in the youngest ages (5-26 days) and the major metabolites in adult testes were testosterone and androstenedione. At 30, 42 and 49 days of age, dihydrotestosterone and small amounts of androstanediol were present. Time-sequence studies indicated that dihydrotestosterone and androstanediol were formed from the 5alpha-reduction (and 3-keto reduction) of testosterone. In a second series of experiments, tissue fragments of a variety of urogenital tract tissues were incubated with [3H]-testosterone and the metabolites separated by HPLC. During the interval in which male urogenital tract differentiation takes place in this species (between Days 15 and 28), the major metabolite identified was dihydrotestosterone. We conclude that the timing of 5alpha-reductase expression in the testes of the grey short-tailed possum resembles that of rodents and the brushtail possum rather than that of the tammar wallaby and that dihydrotestosterone is probably the intracellular androgen responsible for virilisation of the urogenital tract in this species.
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role of the alternate pathway of dihydrotestosterone formation in virilization of the wolffian ducts of the tammar wallaby macropus eugenii
Endocrinology, 2006Co-Authors: Geoffrey Shaw, Jean D Wilson, Richard J Auchus, Jane C Fenelon, Michelle Sichlau, Marilyn B RenfreeAbstract:Dihydrotestosterone in androgen target tissues is formed under most circumstances by the 5alpha-reduction of testosterone, but an alternate pathway involves the oxidation of androstanediol to dihydrotestosterone. To investigate the mechanism by which androgens virilize the Wolffian ducts in the tammar wallaby, [(3)H]progesterone was incubated with testes from d 10 and 19 pouch young, and radioactivity was recovered in testosterone and androstanediol at both ages. Analysis of the intermediates indicates that androstanediol was formed both from testosterone via 5alpha-reduction and 3alpha-keto reduction and directly from 5alpha-reduced progestogens. 5alpha-Reductase activity was high in minces of mesonephros/epididymis from d 6-21 pouch young. When minces of urogenital tract tissues from d 19 pouch young were incubated with [(3)H]testosterone, [(3)H]dihydrotestosterone, and [(3)H]androstanediol, dihydrotestosterone was the principal androgen formed in the mesonephros/epididymis, urogenital sinus, and urogenital tubercle, whereas androstanediol was the principal androgen formed by the testis. In intact pouch young studied between d 10 and 34, administration of the 5alpha-reductase inhibitor, 17beta-(N,N-diethyl)carbamoyl-4-methyl-4-aza-5alpha-androstan-3-one, blocked virilization of the Wolffian ducts in males, and administration of androstanediol caused virilization of the Wolffian ducts in females. We conclude that dihydrotestosterone, largely formed in the tissue by the oxidation of androstanediol derived from the testes and also the 5alpha-reduction of testosterone, is responsible for Wolffian duct virilization in this species.
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steroid 5α reductase 1 promotes 5α androstane 3α 17β diol synthesis in immature mouse testes by two pathways
Molecular and Cellular Endocrinology, 2004Co-Authors: Mala Mahendroo, Jean D Wilson, James A Richardson, Richard J AuchusAbstract:Abstract 5α-Androstane-3α,17β-diol (androstanediol) is the predominant androgen in immature mouse testes, and studies were designed to investigate its pathway of synthesis, the steroid 5α-reductase isoenzyme involved in its formation, and whether testicular androstanediol is formed in embryonic mouse testes at the time of male phenotypic development. In 24–26-day-old immature testes, androstanediol is formed by two pathways; the predominant one involves testosterone → dihydrotestosterone → androstanediol, and a second utilizes the pathway progesterone → 5α-dihydroprogesterone → 5α-pregnane-3α-ol-20-one → 5α-pregnane-3α,17α-diol-20-one → androsterone → androstanediol. Formation of androstanediol was normal in testes from mice deficient in steroid 5α-reductase 2 but absent in testes from mice deficient in steroid 5α-reductase 1, indicating that isoenzyme 2 is not expressed in day 24–26 testes. The fact that androstenedione and testosterone were the only androgens identified after incubation of day 16 and 17 embryonic testes with [ 3 H ]progesterone implies that androstanediol formation in the testis plays no role in male phenotypic differentiation in the mouse.
David E. Wildt - One of the best experts on this subject based on the ideXlab platform.
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urinary 3 alpha 17 beta androstanediol glucuronide is a measure of androgenic status in eld s deer stags cervus eldi thamin
Biology of Reproduction, 1995Co-Authors: Steven L. Monfort, Hollis A. Simmons, E Harvey, Luis R Padilla, L R Williamson, Lucie Geurts, David E. WildtAbstract:To determine the primary excretory by-products of testosterone (T), 85 Ci [3H]T was administered i.v. to two adult Eld's deer stags. Blood (10 ml) was collected by jugular venipuncture at 0, 5, 10, 15, 30, 45, 60, 90, 120, 150, 180, 240, and 480 min after isotope infusion, and all urine and feces were collected for 96 h after injection. Seventy percent of labeled circulating steroid was conjugated by 30 min postinfusion. The majority (80.4 3.2%) of T metabolites were excreted into urine, and 95.0 0.9% of these were conjugated, 95.8 0.2% being hydrolyzable with glucuronidase. Seven urinary androgen metabolites, including androstanediol (5a-androstan-3a-173-diol and 5-androstan-3a-1 70-diol),were identified in glucuronidase-hydrolyzed, ether-extracted Eld's deer urine pools after gas chromatography/ mass spectrometry. A double-antibody 1 25 1 RIA for 5a3?-Androstanediol-3a,173-diol, 17-glucuronide (3a-diol-G) was validated for unprocessed urine. Longitudinal assessments of urine samples collected from 13 stags for 3 yr revealed biological concordance between fluctuations in urinary 3a-diol-G and serum T, as well as seasonal changes in secondary sexual characteristics. Overall correlation between "sameday" matched serum T and urinary 3-diol-G was 0.58, (n = 6; p < 0.001). Thus, monitoring urinary 3a-diol-G provides a noninvasive, alternative method for characterizing male endocrine interrelationships in an endangered ungulate species.
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urinary 3 alpha 17 beta androstanediol glucuronide is a measure of androgenic status in eld s deer stags cervus eldi thamin
Biology of Reproduction, 1995Co-Authors: Steven L Monfort, E Harvey, Luis R Padilla, L R Williamson, Lucie Geurts, H A Simmons, David E. WildtAbstract:To determine the primary excretory by-products of testosterone (T), 85 microCi [3H]T was administered i.v. to two adult Eld's deer stags. Blood (10 ml) was collected by jugular venipuncture at 0, 5, 10, 15, 30, 45, 60, 90, 120, 150, 180, 240, and 480 min after isotope infusion, and all urine and feces were collected for 96 h after injection. Seventy percent of labeled circulating steroid was conjugated by 30 min postinfusion. The majority (80.4 +/- 3.2%) of T metabolites were excreted into urine, and 95.0 +/- 0.9% of these were conjugated, 95.8 +/- 0.2% being hydrolyzable with glucuronidase. Seven urinary androgen metabolites, including androstanediol (5 alpha-androstan-3 alpha-17 beta-diol and 5 beta-androstan-3 alpha-17 beta-diol), were identified in glucoronidase-hydrolyzed, ether-extracted Eld's deer urine pools after gas chromatography/mass spectrometry. A double-antibody 125I RIA for 5 alpha3?-Androstanediol-3 alpha, 17 beta-diol,17-glucuronide (3 alpha-diol-G) was validated for unprocessed urine. Longitudinal assessments of urine samples collected from 13 stages for 3 yr revealed biological concordance between fluctuations in urinary 3 alpha-diol-G and serum T, as well as seasonal changes in secondary sexual characteristics. Overall correlation between "same-day" matched serum T and urinary 3 alpha-diol-G was 0.58, (n = 6; p < 0.001). Thus, monitoring urinary 3 alpha-diol-G provides a noninvasive, alternative method for characterizing male endocrine interrelationships in an endangered ungulate species.
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urinary 3 alpha 17 beta androstanediol glucuronide is a measure of androgenic status in eld s deer stags cervus eldi thamin
Biology of Reproduction, 1995Co-Authors: Steven L. Monfort, Hollis A. Simmons, E Harvey, Luis R Padilla, L R Williamson, Lucie Geurts, David E. WildtAbstract:To determine the primary excretory by-products of testosterone (T), 85 Ci [3H]T was administered i.v. to two adult Eld's deer stags. Blood (10 ml) was collected by jugular venipuncture at 0, 5, 10, 15, 30, 45, 60, 90, 120, 150, 180, 240, and 480 min after isotope infusion, and all urine and feces were collected for 96 h after injection. Seventy percent of labeled circulating steroid was conjugated by 30 min postinfusion. The majority (80.4 3.2%) of T metabolites were excreted into urine, and 95.0 0.9% of these were conjugated, 95.8 0.2% being hydrolyzable with glucuronidase. Seven urinary androgen metabolites, including androstanediol (5a-androstan-3a-173-diol and 5-androstan-3a-1 70-diol),were identified in glucuronidase-hydrolyzed, ether-extracted Eld's deer urine pools after gas chromatography/ mass spectrometry. A double-antibody 1 25 1 RIA for 5a3?-Androstanediol-3a,173-diol, 17-glucuronide (3a-diol-G) was validated for unprocessed urine. Longitudinal assessments of urine samples collected from 13 stags for 3 yr revealed biological concordance between fluctuations in urinary 3a-diol-G and serum T, as well as seasonal changes in secondary sexual characteristics. Overall correlation between "sameday" matched serum T and urinary 3-diol-G was 0.58, (n = 6; p < 0.001). Thus, monitoring urinary 3a-diol-G provides a noninvasive, alternative method for characterizing male endocrine interrelationships in an endangered ungulate species.
Geoffrey Shaw - One of the best experts on this subject based on the ideXlab platform.
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formation of 5α reduced androgens in the testes and urogenital tract of the grey short tailed opossum monodelphis domestica
Reproduction Fertility and Development, 2009Co-Authors: Jean D Wilson, Marilyn B Renfree, Richard J Auchus, Andrew J Pask, Geoffrey ShawAbstract:Testicular 5α-reduced androgens, largely 5α-androstane-3α,17β-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with ( 3 H)-progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). The only 19-carbon metabolites identified in the youngest ages (5-26 days) and the major metabolites in adult testes were testosterone and androstenedione. At 30, 42 and 49 days of age, dihydrotestosterone and small amounts of androstanediol were present. Time-sequence studies indicated that dihydrotestosterone and androstanediol were formed from the 5α-reduction (and 3-keto reduction) of testosterone. In a second series of experiments, tissue fragments of a variety of urogenital tract tissues were incubated with ( 3 H)-testosterone and the metabolites separated by HPLC. During the interval in which male urogenital tract differentiation takes place in this species (between Days 15 and 28), the major metabolite identified was dihydrotestosterone. We conclude that the timing of 5α-reductase expression in the testes of the grey short-tailed possum resembles that of rodents and the brushtail possum rather than that of the tammar wallaby and that dihydrotestosterone is probably the intracellular androgen responsible for virilisation of the urogenital tract in this species.
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formation of 5α reduced androgens in the testes and urogenital tract of the grey short tailed opossum monodelphis domestica
Reproduction Fertility and Development, 2009Co-Authors: Jean D Wilson, Marilyn B Renfree, Richard J Auchus, Andrew J Pask, Geoffrey ShawAbstract:Testicular 5alpha-reduced androgens, largely 5alpha-androstane-3alpha,17beta-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with [3H]-progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). The only 19-carbon metabolites identified in the youngest ages (5-26 days) and the major metabolites in adult testes were testosterone and androstenedione. At 30, 42 and 49 days of age, dihydrotestosterone and small amounts of androstanediol were present. Time-sequence studies indicated that dihydrotestosterone and androstanediol were formed from the 5alpha-reduction (and 3-keto reduction) of testosterone. In a second series of experiments, tissue fragments of a variety of urogenital tract tissues were incubated with [3H]-testosterone and the metabolites separated by HPLC. During the interval in which male urogenital tract differentiation takes place in this species (between Days 15 and 28), the major metabolite identified was dihydrotestosterone. We conclude that the timing of 5alpha-reductase expression in the testes of the grey short-tailed possum resembles that of rodents and the brushtail possum rather than that of the tammar wallaby and that dihydrotestosterone is probably the intracellular androgen responsible for virilisation of the urogenital tract in this species.
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role of the alternate pathway of dihydrotestosterone formation in virilization of the wolffian ducts of the tammar wallaby macropus eugenii
Endocrinology, 2006Co-Authors: Geoffrey Shaw, Jean D Wilson, Richard J Auchus, Jane C Fenelon, Michelle Sichlau, Marilyn B RenfreeAbstract:Dihydrotestosterone in androgen target tissues is formed under most circumstances by the 5alpha-reduction of testosterone, but an alternate pathway involves the oxidation of androstanediol to dihydrotestosterone. To investigate the mechanism by which androgens virilize the Wolffian ducts in the tammar wallaby, [(3)H]progesterone was incubated with testes from d 10 and 19 pouch young, and radioactivity was recovered in testosterone and androstanediol at both ages. Analysis of the intermediates indicates that androstanediol was formed both from testosterone via 5alpha-reduction and 3alpha-keto reduction and directly from 5alpha-reduced progestogens. 5alpha-Reductase activity was high in minces of mesonephros/epididymis from d 6-21 pouch young. When minces of urogenital tract tissues from d 19 pouch young were incubated with [(3)H]testosterone, [(3)H]dihydrotestosterone, and [(3)H]androstanediol, dihydrotestosterone was the principal androgen formed in the mesonephros/epididymis, urogenital sinus, and urogenital tubercle, whereas androstanediol was the principal androgen formed by the testis. In intact pouch young studied between d 10 and 34, administration of the 5alpha-reductase inhibitor, 17beta-(N,N-diethyl)carbamoyl-4-methyl-4-aza-5alpha-androstan-3-one, blocked virilization of the Wolffian ducts in males, and administration of androstanediol caused virilization of the Wolffian ducts in females. We conclude that dihydrotestosterone, largely formed in the tissue by the oxidation of androstanediol derived from the testes and also the 5alpha-reduction of testosterone, is responsible for Wolffian duct virilization in this species.
Richard J Auchus - One of the best experts on this subject based on the ideXlab platform.
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age dependent increases in adrenal cytochrome b5 and serum 5 androstenediol 3 sulfate
The Journal of Clinical Endocrinology and Metabolism, 2016Co-Authors: Juilee Rege, Richard J Auchus, Shigehiro Karashima, Antonio M Lerario, Joshua M Smith, Josephine Z Kasavubu, Hironobu Sasano, Yasuhiro Nakamura, Perrin C WhiteAbstract:Context: Adrenal production of dehydroepiandrosterone sulfate (DHEA-S) increases throughout childhood owing to expansion of the zona reticularis (ZR). ZR features cells with a steroidogenic phenotype distinct from that of the adjacent zona fasciculata, with higher expression of cytochrome b5 type A (CYB5A) and steroid sulfotransferase type 2A1 but decreased 3β-hydroxysteroid dehydrogenase type 2 (HSD3B2). In addition to DHEA-S, three adrenal Δ5-steroid sulfates could provide additional tools to define adrenal maturation. Objective: This study sought to simultaneously measure serum levels of four adrenal Δ5-steroid sulfates, pregnenolone sulfate (Preg-S), 17α-hydroxypregnenolone sulfate (17OHPreg-S), DHEA-S, and 5-androstenediol-3-sulfate (Adiol-S) as a function of age and relate their production to the age-dependent adrenal localization of CYB5A. Participants and Methods: Δ5-steroid sulfates were quantified by liquid chromatography–tandem mass spectrometry in sera from 247 normal children (129 males,118 f...
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formation of 5α reduced androgens in the testes and urogenital tract of the grey short tailed opossum monodelphis domestica
Reproduction Fertility and Development, 2009Co-Authors: Jean D Wilson, Marilyn B Renfree, Richard J Auchus, Andrew J Pask, Geoffrey ShawAbstract:Testicular 5α-reduced androgens, largely 5α-androstane-3α,17β-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with ( 3 H)-progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). The only 19-carbon metabolites identified in the youngest ages (5-26 days) and the major metabolites in adult testes were testosterone and androstenedione. At 30, 42 and 49 days of age, dihydrotestosterone and small amounts of androstanediol were present. Time-sequence studies indicated that dihydrotestosterone and androstanediol were formed from the 5α-reduction (and 3-keto reduction) of testosterone. In a second series of experiments, tissue fragments of a variety of urogenital tract tissues were incubated with ( 3 H)-testosterone and the metabolites separated by HPLC. During the interval in which male urogenital tract differentiation takes place in this species (between Days 15 and 28), the major metabolite identified was dihydrotestosterone. We conclude that the timing of 5α-reductase expression in the testes of the grey short-tailed possum resembles that of rodents and the brushtail possum rather than that of the tammar wallaby and that dihydrotestosterone is probably the intracellular androgen responsible for virilisation of the urogenital tract in this species.
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formation of 5α reduced androgens in the testes and urogenital tract of the grey short tailed opossum monodelphis domestica
Reproduction Fertility and Development, 2009Co-Authors: Jean D Wilson, Marilyn B Renfree, Richard J Auchus, Andrew J Pask, Geoffrey ShawAbstract:Testicular 5alpha-reduced androgens, largely 5alpha-androstane-3alpha,17beta-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with [3H]-progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). The only 19-carbon metabolites identified in the youngest ages (5-26 days) and the major metabolites in adult testes were testosterone and androstenedione. At 30, 42 and 49 days of age, dihydrotestosterone and small amounts of androstanediol were present. Time-sequence studies indicated that dihydrotestosterone and androstanediol were formed from the 5alpha-reduction (and 3-keto reduction) of testosterone. In a second series of experiments, tissue fragments of a variety of urogenital tract tissues were incubated with [3H]-testosterone and the metabolites separated by HPLC. During the interval in which male urogenital tract differentiation takes place in this species (between Days 15 and 28), the major metabolite identified was dihydrotestosterone. We conclude that the timing of 5alpha-reductase expression in the testes of the grey short-tailed possum resembles that of rodents and the brushtail possum rather than that of the tammar wallaby and that dihydrotestosterone is probably the intracellular androgen responsible for virilisation of the urogenital tract in this species.
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role of the alternate pathway of dihydrotestosterone formation in virilization of the wolffian ducts of the tammar wallaby macropus eugenii
Endocrinology, 2006Co-Authors: Geoffrey Shaw, Jean D Wilson, Richard J Auchus, Jane C Fenelon, Michelle Sichlau, Marilyn B RenfreeAbstract:Dihydrotestosterone in androgen target tissues is formed under most circumstances by the 5alpha-reduction of testosterone, but an alternate pathway involves the oxidation of androstanediol to dihydrotestosterone. To investigate the mechanism by which androgens virilize the Wolffian ducts in the tammar wallaby, [(3)H]progesterone was incubated with testes from d 10 and 19 pouch young, and radioactivity was recovered in testosterone and androstanediol at both ages. Analysis of the intermediates indicates that androstanediol was formed both from testosterone via 5alpha-reduction and 3alpha-keto reduction and directly from 5alpha-reduced progestogens. 5alpha-Reductase activity was high in minces of mesonephros/epididymis from d 6-21 pouch young. When minces of urogenital tract tissues from d 19 pouch young were incubated with [(3)H]testosterone, [(3)H]dihydrotestosterone, and [(3)H]androstanediol, dihydrotestosterone was the principal androgen formed in the mesonephros/epididymis, urogenital sinus, and urogenital tubercle, whereas androstanediol was the principal androgen formed by the testis. In intact pouch young studied between d 10 and 34, administration of the 5alpha-reductase inhibitor, 17beta-(N,N-diethyl)carbamoyl-4-methyl-4-aza-5alpha-androstan-3-one, blocked virilization of the Wolffian ducts in males, and administration of androstanediol caused virilization of the Wolffian ducts in females. We conclude that dihydrotestosterone, largely formed in the tissue by the oxidation of androstanediol derived from the testes and also the 5alpha-reduction of testosterone, is responsible for Wolffian duct virilization in this species.
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steroid 5α reductase 1 promotes 5α androstane 3α 17β diol synthesis in immature mouse testes by two pathways
Molecular and Cellular Endocrinology, 2004Co-Authors: Mala Mahendroo, Jean D Wilson, James A Richardson, Richard J AuchusAbstract:Abstract 5α-Androstane-3α,17β-diol (androstanediol) is the predominant androgen in immature mouse testes, and studies were designed to investigate its pathway of synthesis, the steroid 5α-reductase isoenzyme involved in its formation, and whether testicular androstanediol is formed in embryonic mouse testes at the time of male phenotypic development. In 24–26-day-old immature testes, androstanediol is formed by two pathways; the predominant one involves testosterone → dihydrotestosterone → androstanediol, and a second utilizes the pathway progesterone → 5α-dihydroprogesterone → 5α-pregnane-3α-ol-20-one → 5α-pregnane-3α,17α-diol-20-one → androsterone → androstanediol. Formation of androstanediol was normal in testes from mice deficient in steroid 5α-reductase 2 but absent in testes from mice deficient in steroid 5α-reductase 1, indicating that isoenzyme 2 is not expressed in day 24–26 testes. The fact that androstenedione and testosterone were the only androgens identified after incubation of day 16 and 17 embryonic testes with [ 3 H ]progesterone implies that androstanediol formation in the testis plays no role in male phenotypic differentiation in the mouse.
Luis R Padilla - One of the best experts on this subject based on the ideXlab platform.
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urinary 3 alpha 17 beta androstanediol glucuronide is a measure of androgenic status in eld s deer stags cervus eldi thamin
Biology of Reproduction, 1995Co-Authors: Steven L. Monfort, Hollis A. Simmons, E Harvey, Luis R Padilla, L R Williamson, Lucie Geurts, David E. WildtAbstract:To determine the primary excretory by-products of testosterone (T), 85 Ci [3H]T was administered i.v. to two adult Eld's deer stags. Blood (10 ml) was collected by jugular venipuncture at 0, 5, 10, 15, 30, 45, 60, 90, 120, 150, 180, 240, and 480 min after isotope infusion, and all urine and feces were collected for 96 h after injection. Seventy percent of labeled circulating steroid was conjugated by 30 min postinfusion. The majority (80.4 3.2%) of T metabolites were excreted into urine, and 95.0 0.9% of these were conjugated, 95.8 0.2% being hydrolyzable with glucuronidase. Seven urinary androgen metabolites, including androstanediol (5a-androstan-3a-173-diol and 5-androstan-3a-1 70-diol),were identified in glucuronidase-hydrolyzed, ether-extracted Eld's deer urine pools after gas chromatography/ mass spectrometry. A double-antibody 1 25 1 RIA for 5a3?-Androstanediol-3a,173-diol, 17-glucuronide (3a-diol-G) was validated for unprocessed urine. Longitudinal assessments of urine samples collected from 13 stags for 3 yr revealed biological concordance between fluctuations in urinary 3a-diol-G and serum T, as well as seasonal changes in secondary sexual characteristics. Overall correlation between "sameday" matched serum T and urinary 3-diol-G was 0.58, (n = 6; p < 0.001). Thus, monitoring urinary 3a-diol-G provides a noninvasive, alternative method for characterizing male endocrine interrelationships in an endangered ungulate species.
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urinary 3 alpha 17 beta androstanediol glucuronide is a measure of androgenic status in eld s deer stags cervus eldi thamin
Biology of Reproduction, 1995Co-Authors: Steven L Monfort, E Harvey, Luis R Padilla, L R Williamson, Lucie Geurts, H A Simmons, David E. WildtAbstract:To determine the primary excretory by-products of testosterone (T), 85 microCi [3H]T was administered i.v. to two adult Eld's deer stags. Blood (10 ml) was collected by jugular venipuncture at 0, 5, 10, 15, 30, 45, 60, 90, 120, 150, 180, 240, and 480 min after isotope infusion, and all urine and feces were collected for 96 h after injection. Seventy percent of labeled circulating steroid was conjugated by 30 min postinfusion. The majority (80.4 +/- 3.2%) of T metabolites were excreted into urine, and 95.0 +/- 0.9% of these were conjugated, 95.8 +/- 0.2% being hydrolyzable with glucuronidase. Seven urinary androgen metabolites, including androstanediol (5 alpha-androstan-3 alpha-17 beta-diol and 5 beta-androstan-3 alpha-17 beta-diol), were identified in glucoronidase-hydrolyzed, ether-extracted Eld's deer urine pools after gas chromatography/mass spectrometry. A double-antibody 125I RIA for 5 alpha3?-Androstanediol-3 alpha, 17 beta-diol,17-glucuronide (3 alpha-diol-G) was validated for unprocessed urine. Longitudinal assessments of urine samples collected from 13 stages for 3 yr revealed biological concordance between fluctuations in urinary 3 alpha-diol-G and serum T, as well as seasonal changes in secondary sexual characteristics. Overall correlation between "same-day" matched serum T and urinary 3 alpha-diol-G was 0.58, (n = 6; p < 0.001). Thus, monitoring urinary 3 alpha-diol-G provides a noninvasive, alternative method for characterizing male endocrine interrelationships in an endangered ungulate species.
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urinary 3 alpha 17 beta androstanediol glucuronide is a measure of androgenic status in eld s deer stags cervus eldi thamin
Biology of Reproduction, 1995Co-Authors: Steven L. Monfort, Hollis A. Simmons, E Harvey, Luis R Padilla, L R Williamson, Lucie Geurts, David E. WildtAbstract:To determine the primary excretory by-products of testosterone (T), 85 Ci [3H]T was administered i.v. to two adult Eld's deer stags. Blood (10 ml) was collected by jugular venipuncture at 0, 5, 10, 15, 30, 45, 60, 90, 120, 150, 180, 240, and 480 min after isotope infusion, and all urine and feces were collected for 96 h after injection. Seventy percent of labeled circulating steroid was conjugated by 30 min postinfusion. The majority (80.4 3.2%) of T metabolites were excreted into urine, and 95.0 0.9% of these were conjugated, 95.8 0.2% being hydrolyzable with glucuronidase. Seven urinary androgen metabolites, including androstanediol (5a-androstan-3a-173-diol and 5-androstan-3a-1 70-diol),were identified in glucuronidase-hydrolyzed, ether-extracted Eld's deer urine pools after gas chromatography/ mass spectrometry. A double-antibody 1 25 1 RIA for 5a3?-Androstanediol-3a,173-diol, 17-glucuronide (3a-diol-G) was validated for unprocessed urine. Longitudinal assessments of urine samples collected from 13 stags for 3 yr revealed biological concordance between fluctuations in urinary 3a-diol-G and serum T, as well as seasonal changes in secondary sexual characteristics. Overall correlation between "sameday" matched serum T and urinary 3-diol-G was 0.58, (n = 6; p < 0.001). Thus, monitoring urinary 3a-diol-G provides a noninvasive, alternative method for characterizing male endocrine interrelationships in an endangered ungulate species.