3 Hydroxyphenylacetic Acid

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João M. Madurro - One of the best experts on this subject based on the ideXlab platform.

  • modified electrode with reduced graphene oxide poly 3 Hydroxyphenylacetic Acid a new platform for oligonucleotide hybridization
    Journal of Solid State Electrochemistry, 2017
    Co-Authors: Jussara Vieira Da Silva, Ana G B Madurro, João M. Madurro
    Abstract:

    This paper reports a new platform for oligonucleotide hybridization, prepared by electropolymerization of 3-Hydroxyphenylacetic Acid onto gold electrode modified with reduced graphene oxide. Electrochemical polymerization indicated that reduced graphene oxide was able to oxidize the monomer in lower potential than the gold electrode. Their structural, morphological, and electrochemical properties were evaluated by Fourier transform infrared spectroscopy, elemental analysis, atomic force microscopy, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The new platform exhibited high efficiency for immobilization and hybridization detection of oligonucleotides through both direct electrochemical oxidation of guanine residues and indirect oxidation of the electroactive intercalator, ethidium bromide. The genosensor could detect the complementary sequence with a detection limit of 10.4 pmol L−1.

  • Electropolymerization of Hydroxyphenylacetic Acid isomers and the development of a bioelectrode for the diagnosis of bacterial meningitis
    Journal of Applied Electrochemistry, 2015
    Co-Authors: Luciano Pereira Rodrigues, Deusmaque Carneiro Ferreira, Lucas Franco Ferreira, Sara Cuadros-orellana, Guilherme Corrêa Oliveira, Ana G. Brito-madurro, Ronaldo Junio Oliveira, Odonírio Abrahão, João M. Madurro
    Abstract:

    In this work, three polymeric films derived from Hydroxyphenylacetic Acid isomers were electropolymerized onto the surfaces of graphite electrodes through cyclic voltammetry. Analysis of electrochemical behaviors and electrical properties of the three polymeric films were investigated and their probably structures were explored by molecular modeling. The best functionalization strategy for the incorporation and retention of adenine and guanine nitrogenous bases was the poly(3-Hydroxyphenylacetic Acid). It was applied for the immobilization of a nucleotide probe specific to DNA of the bacteria Neisseria meningitidis operated in amperometric and impedimetric indirect modes. The simple protocol of electrochemical detection of DNA by the bioelectrode, developed in this work, could be further enhanced and applied in a low-cost and pain-less platform to diagnose human meningitis infection.

  • molecular modeling study on the possible polymers formed during the electropolymerization of 3 Hydroxyphenylacetic Acid
    Journal of Molecular Graphics & Modelling, 2012
    Co-Authors: Deusmaque Carneiro Ferreira, João M. Madurro, Ana G B Madurro, Antonio E H Machado, Fernanda De Souza Tiago, Odonírio Abrahão
    Abstract:

    The compound 3-Hydroxyphenylacetic Acid (3-HPA) has been used as a monomer in the synthesis of polymeric films by electropolymerization; these films serve as supports for the immobilization of biomolecules in electrochemical biosensors. To assist in the elucidation of the mechanism of 3-HPA electropolymerization, a systematic quantum mechanical study was conducted. In addition to the monomer, all possible intermediates and the probable oligomers formed during the electropolymerization were investigated using a density functional theory (DFT) method combined with a previous conformational analysis performed with the aid of the RM1 semi-empirical method or a Monte Carlo conformational analysis with the force field OPLS-2005. From the data analysis combined with the experimental results, a mechanism was proposed for the main route of formation of the polymeric films. The mechanism involves the formation of polyethers from the coupling of phenoxide radicals and radicals based on the aromatic ring.

  • electrochemical and morphological studies of an electroactive material derived from 3 Hydroxyphenylacetic Acid a new matrix for oligonucleotide hybridization
    Journal of Materials Science, 2010
    Co-Authors: Ronalt M L Oliveira, Lucas Franco Ferreira, Sabrina Nunes Vieira, Heden C S Alves, Erick G Franca, Diego Leoni Franco, Ana G Britomadurro, João M. Madurro
    Abstract:

    This paper describes the formation of polymeric films derived from 3-Hydroxyphenylacetic Acid electropolymerized onto graphite electrodes through cyclic voltammetry. We observed the formation of an electroactive material over the electrode surface. The modified electrode showed significant blocking behavior to electron transfer reaction of the pair redox ferricyanide/ferrocyanide, indicating repulsion electrostatic with the negatively charged carboxylate groups of the polymer. The quasi-reversible behavior to Ru(NH3)6Cl2 suggests electrostatic attraction, facilitating the charge transfer. The modified electrode was studied through electrochemical quartz crystal microbalance, electrochemical impedance spectroscopy, and atomic force microscopy. These analyses indicate modification of the graphite electrode. Surface analysis by AFM showed that the morphology of the modified electrode surface presents globular form, randomly distributed, and formed by lower globules with diameter near 100 nm. Immobilization and hybridization of oligonucleotide onto the modified electrode were successfully carried out by using both direct electrochemical oxidation of nitrogenated bases and the redox electroactive indicator methylene blue.

Ying Peng - One of the best experts on this subject based on the ideXlab platform.

  • urinary 3 3 hydroxyphenyl 3 hydroxypropionic Acid 3 Hydroxyphenylacetic Acid and 3 hydroxyhippuric Acid are elevated in children with autism spectrum disorders
    BioMed Research International, 2016
    Co-Authors: Xiyue Xiong, Dan Liu, Yichao Wang, Ting Zeng, Ying Peng
    Abstract:

    Autism spectrum disorders (ASDs) are a group of mental illnesses highly correlated with gut microbiota. Recent studies have shown that some abnormal aromatic metabolites in autism patients are presumably derived from overgrown Clostridium species in gut, which may be used for diagnostic purposes. In this paper, a GC/MS based metabolomic approach was utilized to seek similar biomarkers by analyzing the urinary information in 62 ASDs patients compared with 62 non-ASDs controls in China, aged 1.5-7. Three compounds identified as 3-(3-hydroxyphenyl)-3-hydroxypropionic Acid (HPHPA), 3-Hydroxyphenylacetic Acid (3HPA), and 3-hydroxyhippuric Acid (3HHA) were found in higher concentrations in autistic children than in the controls (p 96%). After two-regression analysis, the optimal area under the curve (AUC, 0.962), sensitivity (90.3%), and specificity (98.4%) were obtained by ROC curve of Prediction probability based on the three metabolites. These findings demonstrate that the measurements of the three compounds are strong predictors of ASDs and support the potential clinical utility for identifying a subgroup of ASDs subjects.

  • Urinary 3-(3-Hydroxyphenyl)-3-hydroxypropionic Acid, 3-Hydroxyphenylacetic Acid, and 3-Hydroxyhippuric Acid Are Elevated in Children with Autism Spectrum Disorders
    Hindawi Limited, 2016
    Co-Authors: Xiyue Xiong, Dan Liu, Yichao Wang, Ting Zeng, Ying Peng
    Abstract:

    Autism spectrum disorders (ASDs) are a group of mental illnesses highly correlated with gut microbiota. Recent studies have shown that some abnormal aromatic metabolites in autism patients are presumably derived from overgrown Clostridium species in gut, which may be used for diagnostic purposes. In this paper, a GC/MS based metabolomic approach was utilized to seek similar biomarkers by analyzing the urinary information in 62 ASDs patients compared with 62 non-ASDs controls in China, aged 1.5–7. Three compounds identified as 3-(3-hydroxyphenyl)-3-hydroxypropionic Acid (HPHPA), 3-Hydroxyphenylacetic Acid (3HPA), and 3-hydroxyhippuric Acid (3HHA) were found in higher concentrations in autistic children than in the controls (p

Harry Gruppe - One of the best experts on this subject based on the ideXlab platform.

  • procyanidin dimers are metabolized by human microbiota with 2 3 4 dihydroxyphenyl acetic Acid and 5 3 4 dihydroxyphenyl γ valerolactone as the major metabolites
    Journal of Agricultural and Food Chemistry, 2009
    Co-Authors: Maaike M Appeldoo, Jeanpaul Vincke, Annamarja Aura, Pete C H Hollma, Harry Gruppe
    Abstract:

    Procyanidins (PCs) are highly abundant phenolic compounds in the human diet and might be responsible for the health effects of chocolate and wine. Due to low absorption of intact PCs, microbial metabolism might play an important role. So far, only a few studies, with crude extracts rich in PCs but also containing a multitude of other phenolic compounds, have been performed to reveal human microbial PC metabolites. Therefore, the origin of the metabolites remains questionable. This study included in vitro fermentation of purified PC dimers with human microbiota. The main metabolites identified were 2-(3,4-dihydroxyphenyl)acetic Acid and 5-(3,4-dihydroxyphenyl)-γ-valerolactone. Other metabolites detected were 3-Hydroxyphenylacetic Acid, 4-Hydroxyphenylacetic Acid, 3-hydroxyphenylpropionic Acid, phenylvaleric Acids, monohydroxylated phenylvalerolactone, and 1-(3′,4′-dihydroxyphenyl)-3-(2′′,4′′,6′′-trihydroxyphenyl)propan-2-ol. Metabolites that could be quantified accounted for at least 12 mol % of the dimers...

Alan Crozier - One of the best experts on this subject based on the ideXlab platform.

  • yoghurt impacts on the excretion of phenolic Acids derived from colonic breakdown of orange juice flavanones in humans
    Molecular Nutrition & Food Research, 2009
    Co-Authors: Suri Roowi, William Mullen, Christine A Edwards, Alan Crozier
    Abstract:

    : Human urine was collected over a 24 h period after the consumption of 250 mL of (i) water, (ii) orange juice, and (iii) orange juice plus 150 mL of full fat natural yoghurt. The orange juice contained 168 micromol of hesperetin-7-O-rutinoside and 18 micromol of naringenin-7-O-rutinoside. GC-MS analysis of the urine identified nine phenolic Acids, five of which, 3-Hydroxyphenylacetic Acid, 3-hydroxyphenylhydracrylic Acid, dihydroferulic Acid, 3-methoxy-4-hydroxyphenylhydracrylic Acid and 3-hydroxyhippuric Acid, were associated with orange juice consumption indicating that they were derived from colonic catabolism of hesperetin-7-O-rutinoside. The overall 0-24 h excretion of the five phenolic Acids was 6.7 +/- 1.8 micromol after drinking water and this increased significantly (p < 0.05) to 62 +/- 18 micromol, equivalent to 37% of the ingested flavanones, following orange juice consumption. When the orange juice was ingested with yoghurt excretion fell back markedly to 9.3 +/- 4.4 micromol. This was not due to a difference in mouth to caecum transit time, as measured with breath hydrogen production, though possibly there may have been a slowing of the bulk of the meal reaching the large intestine which may then have altered the catabolism of the flavanones to phenolic Acids by the colonic microbiota.

  • bioavailability of 2 14c quercetin 4 glucoside in rats
    Journal of Agricultural and Food Chemistry, 2008
    Co-Authors: William Mullen, Christine A Edwards, Jeanmax Rouanet, Cyril Auger, Pierrelouis Teissedre, Stuart T Caldwell, Richard C Hartley, Micheal E J Lean, Alan Crozier
    Abstract:

    [2-(14)C]quercetin-4'-glucoside (4 mg/kg body weight) was fed by gavage to rats housed in metabolic cages, and over an ensuing 72 h period, radiolabeled products in body tissues, plasma, feces, and urine were monitored by high-performance liquid chromatography with online radioactivity and MS2 detection. One and 6 h after ingestion, while in the small intestine, the flavonol glucoside was converted to glucuronide and methylated and sulfated derivatives of quercetin, but only trace amounts of these metabolites were excreted in urine. On entering the cecum and the colon, the flavonol metabolites declined as they were converted to phenolic Acids, principally 3-Hydroxyphenylacetic Acid and 3,4-diHydroxyphenylacetic Acid, by the colonic microflora. Feces contained mainly 3-Hydroxyphenylacetic Acid. Urine collected 0-12 and 0-24 h after ingestion contained radiolabeled hippuric Acid and 3-Hydroxyphenylacetic Acid. 14C-Hippuric Acid declined markedly in the 24-48 and 48-72 h urine samples, and there was a concomitant increase in labeled benzoic Acid. There was minimal accumulation of radioactivity in plasma, despite a 69% recovery of label in urine over the 72 h period, and likewise, very little radioactivity was detected in body tissues out with the gastrointestinal tract. This is reflected in the fact that 72 h after ingestion 96% of the ingested radioactivity was recovered in feces, urine, and the cage washes, which comprise a mixture of urine and feces. The study reveals that as it passes through the gastrointestinal tract, almost all of the of [2-(14)C]quercetin-4'-glucoside is converted to phenolic Acids, compounds not monitored in previous flavonol bioavailability studies with model animal systems, some of which have used exceedingly high doses of the aglycone quercetin (500 mg/kg body weight), which is not a normal dietary component.

  • the relative contribution of the small and large intestine to the absorption and metabolism of rutin in man
    Free Radical Research, 2006
    Co-Authors: Indu Bala Jaganath, William Mullen, Christine A Edwards, Alan Crozier
    Abstract:

    Tomato juice containing rutin (quercetin-3-rutinoside) was ingested by healthy volunteers and ileostomists. Blood and urine collected over 24 h were analysed by HPLC with photodiode array (PDA) and tandem mass spectrometric detection. Low concentrations of isorhamnetin-3-glucuronide (Cmax = 4.3 +/- 1.5 nmoles/l) and quercetin-3-glucuronide (Cmax = 12 +/- 2 nmoles/l) were detected in plasma of healthy subjects. Metabolites appeared in blood after 4 h indicating absorption from the large intestine. Nine metabolites of rutin were detected in urine but with considerable variation in total amount (40 +/- 1-4981 +/- 115 nmoles over 24 h). No metabolites were detected in plasma or urine of ileostomists and 86 +/- 3% of the ingested rutin was recovered in ileal fluid. In subjects with an intact large intestine, but not ileostomists, rutin was catabolised with the appearance of 3,4-diHydroxyphenylacetic Acid, 3-methoxy-4-Hydroxyphenylacetic Acid and 3-Hydroxyphenylacetic Acid in urine accounting for 22% of rutin intake.

Xiyue Xiong - One of the best experts on this subject based on the ideXlab platform.

  • urinary 3 3 hydroxyphenyl 3 hydroxypropionic Acid 3 Hydroxyphenylacetic Acid and 3 hydroxyhippuric Acid are elevated in children with autism spectrum disorders
    BioMed Research International, 2016
    Co-Authors: Xiyue Xiong, Dan Liu, Yichao Wang, Ting Zeng, Ying Peng
    Abstract:

    Autism spectrum disorders (ASDs) are a group of mental illnesses highly correlated with gut microbiota. Recent studies have shown that some abnormal aromatic metabolites in autism patients are presumably derived from overgrown Clostridium species in gut, which may be used for diagnostic purposes. In this paper, a GC/MS based metabolomic approach was utilized to seek similar biomarkers by analyzing the urinary information in 62 ASDs patients compared with 62 non-ASDs controls in China, aged 1.5-7. Three compounds identified as 3-(3-hydroxyphenyl)-3-hydroxypropionic Acid (HPHPA), 3-Hydroxyphenylacetic Acid (3HPA), and 3-hydroxyhippuric Acid (3HHA) were found in higher concentrations in autistic children than in the controls (p 96%). After two-regression analysis, the optimal area under the curve (AUC, 0.962), sensitivity (90.3%), and specificity (98.4%) were obtained by ROC curve of Prediction probability based on the three metabolites. These findings demonstrate that the measurements of the three compounds are strong predictors of ASDs and support the potential clinical utility for identifying a subgroup of ASDs subjects.

  • Urinary 3-(3-Hydroxyphenyl)-3-hydroxypropionic Acid, 3-Hydroxyphenylacetic Acid, and 3-Hydroxyhippuric Acid Are Elevated in Children with Autism Spectrum Disorders
    Hindawi Limited, 2016
    Co-Authors: Xiyue Xiong, Dan Liu, Yichao Wang, Ting Zeng, Ying Peng
    Abstract:

    Autism spectrum disorders (ASDs) are a group of mental illnesses highly correlated with gut microbiota. Recent studies have shown that some abnormal aromatic metabolites in autism patients are presumably derived from overgrown Clostridium species in gut, which may be used for diagnostic purposes. In this paper, a GC/MS based metabolomic approach was utilized to seek similar biomarkers by analyzing the urinary information in 62 ASDs patients compared with 62 non-ASDs controls in China, aged 1.5–7. Three compounds identified as 3-(3-hydroxyphenyl)-3-hydroxypropionic Acid (HPHPA), 3-Hydroxyphenylacetic Acid (3HPA), and 3-hydroxyhippuric Acid (3HHA) were found in higher concentrations in autistic children than in the controls (p