3 Methylcholanthrene

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Nak Doo Kim - One of the best experts on this subject based on the ideXlab platform.

  • Sex-related differences in rat hepatic cytochromes P450 expression following treatment with phenobarbital or 3-Methylcholanthrene
    Archives of Pharmacal Research, 1992
    Co-Authors: Yoon Sook Lee, Sang Shin Park, Nak Doo Kim
    Abstract:

    The induction of hepatic cytochromes P450 and metabolic effects have been examined in male and female Sprague-Dawley rats following treatment with either phenobarbital or 3-Methylcholanthrene. Hepatic cytochrome P450 levels were higher in males than in females by ≈40%. Treatment of male and female rats with phenobarbital or 3-Methylcholanthrene resulted in an ≈1.6- and 2-fold increase, respectively, in heptic microsomal cytochrome P450 levels in both sexes, relative to untreated animals. Immunoblot analyses were performed to compare sex-related changes in P450 levels. Hepatic P450IIB1 levels in males were greater than those in females following phenobarbital treatment. 3-Methylcholanthrene-induced male hepatic microsomes exhibited greater levels of P450 IA1 and IA2 than female microsomes, whereas uninduced microsomes from males or females failed to exhibit a band. Mab PCN 2-13-1 against P450IIIA recognized an intense band in uninduced hepatic microsomes from males whereas no band was recognized in uninduced microsomes from female rats. The levels of P450IIIA in males were increased 2 to 3-fold following treatment with phenobarbital. while the increase of IIIA levels in females by phenobarbital was minimal, as monitored by immunoblot analysis. Solid phase radioimmunoassay using monoclonal antibodies supported the results of immunoblot analysis. Phenobarbital treatment caused a 6.5-fold increase in the monoclonal antibody binding to IIB1 in males, whereas treatment of females with phenobarbital resulted in a 12-fold increase of IIB1 binding, relative to respective controls. The relative increase of IA levels by 3-Methylcholanthrene was also greater in females than in males (10-vs. 8-fold) although the levels of induced IA were comparable in both sexes, as assessed by radioimmunoassay. Radioimmunoassay also showed that hepatic IIE1 level was 1.5-fold higher in males than in females and that either phenobarbital or 3-Methylcholanthrene treatment caused 80% to 40% decrease in IIE1 levels, relative to control, in both sexes. Sex-related metabolic activities were examined in hepatic microsomes. Hexobarbital hydroxylase activity was 2- to 3-fold higher in uninduced microsomes from males than that from females. This hydroxylase activity was increased 2- and 3-fold in males and females, respectively, following phenobarbital treatment, as compared to controls. Addition of anti-P450IIB1 antibody to phenobarbital-induced hepatic microsomes from males and females produced 64% and 84% inhibition of hexobarbital oxidation, respectively. Aryl hydrocarbon hydroxylase activity was increased ≈12- and 26-fold in males and females. respectively, following 3-Methylcholanthrene treatment relative to controls. The anti-P450IA antibody inhibitable rate of aryl hydrocarbon hydroxylase activity was comparable in both sexes following 3-Methylcholanthrene treatment (≈70%). These results demonstrate that levels of hepatic P450IIB1 or P450IA are greater in male than in female for untreated, phenobarbital- or 3-Methylcholanthrene treated rats. In addition, the relative increase of P450IIB1 or IA by phenobarbital or 3-Methylcholanthrene is more significant in females.

Yoon Sook Lee - One of the best experts on this subject based on the ideXlab platform.

  • Sex-related differences in rat hepatic cytochromes P450 expression following treatment with phenobarbital or 3-Methylcholanthrene
    Archives of Pharmacal Research, 1992
    Co-Authors: Yoon Sook Lee, Sang Shin Park, Nak Doo Kim
    Abstract:

    The induction of hepatic cytochromes P450 and metabolic effects have been examined in male and female Sprague-Dawley rats following treatment with either phenobarbital or 3-Methylcholanthrene. Hepatic cytochrome P450 levels were higher in males than in females by ≈40%. Treatment of male and female rats with phenobarbital or 3-Methylcholanthrene resulted in an ≈1.6- and 2-fold increase, respectively, in heptic microsomal cytochrome P450 levels in both sexes, relative to untreated animals. Immunoblot analyses were performed to compare sex-related changes in P450 levels. Hepatic P450IIB1 levels in males were greater than those in females following phenobarbital treatment. 3-Methylcholanthrene-induced male hepatic microsomes exhibited greater levels of P450 IA1 and IA2 than female microsomes, whereas uninduced microsomes from males or females failed to exhibit a band. Mab PCN 2-13-1 against P450IIIA recognized an intense band in uninduced hepatic microsomes from males whereas no band was recognized in uninduced microsomes from female rats. The levels of P450IIIA in males were increased 2 to 3-fold following treatment with phenobarbital. while the increase of IIIA levels in females by phenobarbital was minimal, as monitored by immunoblot analysis. Solid phase radioimmunoassay using monoclonal antibodies supported the results of immunoblot analysis. Phenobarbital treatment caused a 6.5-fold increase in the monoclonal antibody binding to IIB1 in males, whereas treatment of females with phenobarbital resulted in a 12-fold increase of IIB1 binding, relative to respective controls. The relative increase of IA levels by 3-Methylcholanthrene was also greater in females than in males (10-vs. 8-fold) although the levels of induced IA were comparable in both sexes, as assessed by radioimmunoassay. Radioimmunoassay also showed that hepatic IIE1 level was 1.5-fold higher in males than in females and that either phenobarbital or 3-Methylcholanthrene treatment caused 80% to 40% decrease in IIE1 levels, relative to control, in both sexes. Sex-related metabolic activities were examined in hepatic microsomes. Hexobarbital hydroxylase activity was 2- to 3-fold higher in uninduced microsomes from males than that from females. This hydroxylase activity was increased 2- and 3-fold in males and females, respectively, following phenobarbital treatment, as compared to controls. Addition of anti-P450IIB1 antibody to phenobarbital-induced hepatic microsomes from males and females produced 64% and 84% inhibition of hexobarbital oxidation, respectively. Aryl hydrocarbon hydroxylase activity was increased ≈12- and 26-fold in males and females. respectively, following 3-Methylcholanthrene treatment relative to controls. The anti-P450IA antibody inhibitable rate of aryl hydrocarbon hydroxylase activity was comparable in both sexes following 3-Methylcholanthrene treatment (≈70%). These results demonstrate that levels of hepatic P450IIB1 or P450IA are greater in male than in female for untreated, phenobarbital- or 3-Methylcholanthrene treated rats. In addition, the relative increase of P450IIB1 or IA by phenobarbital or 3-Methylcholanthrene is more significant in females.

Emiko Suzuki - One of the best experts on this subject based on the ideXlab platform.

  • Dose of 3-Methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells.
    Journal of biochemical and molecular toxicology, 2011
    Co-Authors: Yasuko Sone, Etsuko Ueta, Yasuko Sannomaru, Noriko Miyake, Hirohito Sone, Yuzuru Otsuka, Kazuo Kondo, Tadao Kurata, Emiko Suzuki
    Abstract:

    Polycyclic aromatic hydrocarbon (PAH) compounds including 3-Methylcholanthrene induce harmful reactive intermediates and reactive oxygen species. This study reports the effect of 3-Methylcholanthrene on the accumulation of vitamin C and the expression of vitamin C transporters. ODS rats were given l-ascorbic acid daily and intraperitoneal injections of 10 mg 3-Methylcholanthrene in total. On day 10, vitamin C concentrations and the expression of vitamin C transporter in the tissues were measured. As a result, the levels of sodium-dependent vitamin C transporter (SVCTs) 1 and the l-ascorbic acid concentration in 3-Methylcholanthrene-treated livers and hepatocytes have increased significantly. However, the content of vitamin C in the urine and TBARS in the liver have not changed. These results suggest that the administration of 3-Methylcholanthrene elevates the requirement for vitamin C via (SVCTs) 1 due to xenobitics-metabolizing, such as the induction of cytochrome P450 family.

F. Berthou - One of the best experts on this subject based on the ideXlab platform.

  • CYP1A2 and 2E1 expression in rat liver treated with combined inducers (3-Methylcholanthrene and ethanol).
    Biochemical and biophysical research communications, 1995
    Co-Authors: T. Goasduff, J.f. Menez, Y. Dreano, F. Berthou
    Abstract:

    The effects of combined ethanol and 3-Methylcholanthrene treatment on rat hepatic cytochrome CYP1A2 and CYP2E1 expression were evaluated. Such a treatment attempts to mimic the simultaneous consumption of ethanol and cigarette smoke. Treatments involving 3-Methylcholanthrene and combined ethanol + 3-Methylcholanthrene decreased both CYP2E1 expression at the mRNA level (0.6 and 0.4 fold versus controls, respectively) and protein level (0.6 and 0.9 fold versus controls, respectively), while dramatically increasing CYP1A2 expression. Furthermore, combined treatment provokes a synergistic induction of CYP1A2 expression as determined by its catalytic activity and protein content.

Sang Shin Park - One of the best experts on this subject based on the ideXlab platform.

  • Sex-related differences in rat hepatic cytochromes P450 expression following treatment with phenobarbital or 3-Methylcholanthrene
    Archives of Pharmacal Research, 1992
    Co-Authors: Yoon Sook Lee, Sang Shin Park, Nak Doo Kim
    Abstract:

    The induction of hepatic cytochromes P450 and metabolic effects have been examined in male and female Sprague-Dawley rats following treatment with either phenobarbital or 3-Methylcholanthrene. Hepatic cytochrome P450 levels were higher in males than in females by ≈40%. Treatment of male and female rats with phenobarbital or 3-Methylcholanthrene resulted in an ≈1.6- and 2-fold increase, respectively, in heptic microsomal cytochrome P450 levels in both sexes, relative to untreated animals. Immunoblot analyses were performed to compare sex-related changes in P450 levels. Hepatic P450IIB1 levels in males were greater than those in females following phenobarbital treatment. 3-Methylcholanthrene-induced male hepatic microsomes exhibited greater levels of P450 IA1 and IA2 than female microsomes, whereas uninduced microsomes from males or females failed to exhibit a band. Mab PCN 2-13-1 against P450IIIA recognized an intense band in uninduced hepatic microsomes from males whereas no band was recognized in uninduced microsomes from female rats. The levels of P450IIIA in males were increased 2 to 3-fold following treatment with phenobarbital. while the increase of IIIA levels in females by phenobarbital was minimal, as monitored by immunoblot analysis. Solid phase radioimmunoassay using monoclonal antibodies supported the results of immunoblot analysis. Phenobarbital treatment caused a 6.5-fold increase in the monoclonal antibody binding to IIB1 in males, whereas treatment of females with phenobarbital resulted in a 12-fold increase of IIB1 binding, relative to respective controls. The relative increase of IA levels by 3-Methylcholanthrene was also greater in females than in males (10-vs. 8-fold) although the levels of induced IA were comparable in both sexes, as assessed by radioimmunoassay. Radioimmunoassay also showed that hepatic IIE1 level was 1.5-fold higher in males than in females and that either phenobarbital or 3-Methylcholanthrene treatment caused 80% to 40% decrease in IIE1 levels, relative to control, in both sexes. Sex-related metabolic activities were examined in hepatic microsomes. Hexobarbital hydroxylase activity was 2- to 3-fold higher in uninduced microsomes from males than that from females. This hydroxylase activity was increased 2- and 3-fold in males and females, respectively, following phenobarbital treatment, as compared to controls. Addition of anti-P450IIB1 antibody to phenobarbital-induced hepatic microsomes from males and females produced 64% and 84% inhibition of hexobarbital oxidation, respectively. Aryl hydrocarbon hydroxylase activity was increased ≈12- and 26-fold in males and females. respectively, following 3-Methylcholanthrene treatment relative to controls. The anti-P450IA antibody inhibitable rate of aryl hydrocarbon hydroxylase activity was comparable in both sexes following 3-Methylcholanthrene treatment (≈70%). These results demonstrate that levels of hepatic P450IIB1 or P450IA are greater in male than in female for untreated, phenobarbital- or 3-Methylcholanthrene treated rats. In addition, the relative increase of P450IIB1 or IA by phenobarbital or 3-Methylcholanthrene is more significant in females.