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Alaa El-din H. Sayed – One of the best experts on this subject based on the ideXlab platform.
Protective effect of p53 knockout on 4-Nonylphenol-induced nephrotoxicity in medaka (Oryzias latipes).Chemosphere, 2019Co-Authors: Alaa El-din H. Sayed, Ahmed Kotb, Shoji Oda, Shosaku Kashiwada, Hiroshi MitaniAbstract:
Abstract In the past few decades, environmental pollutants have become common because of misused nonionic surfactants and detergents. Nonylphenol ethoxylates (NPs) are one of the most important contaminants of water. Therefore, the present study aimed to investigate the protective blocking effect of apoptosis (deficient P53 gene) on 4-Nonylphenol (4-NP)-induced nephrotoxicity of medaka (Oryzias latipes). We divided 36 fish into six groups: two different control groups of wild type (Wt; Hd-rR) control and p53 (−/−) control, and four different treated with 4-Nonylphenol (50 μg/L and 100 μg/L) for 15 days. Histology, immunochemistry, and TUNEL assays confirmed that 4-NP causes nephrotoxicity. Our results showed that 4-NP administration significantly disturbed the kidney structure and function and 4-NP-treated fish showed dilated glomerular vessels, had less glomerular cellular content, decreased expression of glomerular proteins, and an increased level of apoptosis compared with a Wt control group (P
Protective effect of Nigella sativa on 4-Nonylphenol-induced nephrotoxicity in Clarias gariepinus (Burchell, 1822)The Science of the total environment, 2017Co-Authors: Ahmed Kotb, Mahmoud Abd-elkareem, Nasser S. Abou Khalil, Alaa El-din H. SayedAbstract:
Abstract The aim of this study was to examine the protective effects of Nigella sativa ( N. sativa ) on 4-Nonylphenol-induced nephrotoxicity in Clarias gariepinus . 30 fishes were divided into five groups: control, 4-Nonylphenol-treated, 1% N. sativa treated, 2.5% N. sativa treated, and 5% N. sativa treated. N. sativa and 4-Nonylphenol were given for 3 weeks. 4-NP and 4-NP- N. sativa treated fishes were compared with the control group. Kidney histology, immunochemistry, and electron microscope were assessed after 4-NP exposure. In the African catfish, 4-NP is mainly excreted through the kidney causing nephrotoxicity. Our results showed that 4-NP administration significantly disturbed the kidney structure and function. 4-NP treated fishes showed dilated glomerular vessels, fewer glomerular cells content, decreased expressions of glomerular proteins, and increased level of autophagy compared to control group ( P N. sativa has different immunological and pharmacological effects such as anti-apoptotic and anti-oxidant, therefore, the administration of N. sativa with 4-Nonylphenol significantly minimize the nephrotoxic effect of 4-NP and maintain the normal kidney structure and function. Our novel study demonstrated for the first time that N. sativa could protect the kidney against 4-NP induced-nephrotoxicity.
antioxidant and antiapoptotic activities of calotropis procera latex on catfish clarias gariepinus exposed to toxic 4 nonylphenolEcotoxicology and Environmental Safety, 2016Co-Authors: Alaa El-din H. Sayed, Nadia H. Mohamed, M. A. Ismail, Wael M Abdelmageed, Ahmed Am ShoreitAbstract:
Calotropis procera L. is known as medicinal plant. The Phytochemical analyzes of its latex revealed that it possessed antioxidants, namely terpenes, phenolic compounds and cardenolides, flavonoids and saponins, while tannins, alkaloids and resin were absent in moderate to high concentration. In the present study, the role of latex of Calotropis procera as antioxidant and antiapoptotic was reported. To carry out this aim, fishes were exposed to 100 µg l(-1) 4-Nonylphenol as chemical pollutant. The enzymes, superoxidase dismutase, catalase, acetlycholinstrase (AchE), glutathione s-transferase, cortisol, G6PDH) and apoptotic cells increased significantly (p<0.05) accompanied by irregular disturbance of (Na(+), K(+)) ions in the presence of 4-Nonylphenol. On the other hand, these enzymes, ions, and apoptotic cells decreased normally and significantly (p<0.05) in the presence of latex. Total phenol content, total capacity antioxidant, reducing power decrease significantly (p<0.05) in the presence of 4-Nonylphenol and increase normally in the presence of latex. Latex was used for the first time to protect catfish after 4-Nonylphenol exposure. Our study confirms that crude latex of Calotropis procera possessed antioxidant and antiapoptotic activities against the toxicity of 4-Nonylphenol.
Wilhelm Püttmann – One of the best experts on this subject based on the ideXlab platform.
Occurrence and temporal variations of the xenoestrogens bisphenol A, 4-tert-octylphenol, and tech. 4-Nonylphenol in two German wastewater treatment plantsEnvironmental Science and Pollution Research, 2008Co-Authors: Cornelia Höhne, Wilhelm PüttmannAbstract:
Goal, scope, and background The xenoestrogens bisphenol A, 4- tert -octylphenol, and the technical isomer mixture of 4-Nonylphenol (tech. 4-Nonylphenol) belong to the group of chemicals which are called endocrine disrupters due to their property of causing hormonal dysfunctions in the endocrine system of organisms at very low concentrations. Bisphenol A, 4- tert -octylphenol, and the tech. 4-Nonylphenol (mixture of isomers) were determined in water samples collected from the influent and effluent of two German wastewater treatment plants (WWTP) during a long-time sampling period from February 2003 till August 2005 to assess their occurrence and temporal variations in WWTPs. Methods The compounds were extracted and concentrated from water by solid-phase extraction (SPE) using Bond Elut PPL cartridges and quantified by use of gas chromatography–mass spectrometry (GC–MS). Results The influent concentrations were as follows: Bisphenol A < limit of detection of the method (< ldm)—12,205 ng L^−1, tech. 4-Nonylphenol < ldm—10,186 ng L^−1, and 4- tert -octylphenol 39—1,495 ng L^−1. The measured effluent concentrations were lower with values in the range of < ldm—7,625 ng L^−1 for bisphenol A, < ldm—14,444 ng L^−1 for tech. 4-Nonylphenol, and < ldm—392 ng L^−1 for 4- tert -octylphenol. All target compounds were largely eliminated during the wastewater treatment process. The elimination efficiency varied between 73% and 93%. Discussion All analytes show highly fluctuating influent concentrations with very high peak concentrations at particular sampling times. The variation of effluent concentrations is by far lower than the variation of influent concentrations. For tech. 4-Nonylphenol, a significant temporal concentration variation has been detected with very high concentrations up to the microgram-per-liter level in the time from February 2003 till July 2003 and clearly decreasing concentrations in the time from June 2004 till August 2005. This corresponds well with the implementation of Directive 2003/53/EC (nonylphenol and nonylphenol ethoxylates in the European Union “may not be placed on the marked or used as a substance or constituent of preparations in concentrations equal or higher than 0.1% by mass”) from January 2005 on. Bisphenol A is present in the effluent samples in a wide range of concentrations from below the detection limit to high concentrations up to the microgram-per-liter level. For 4- tert -octylphenol, no particular trend of concentration development has been observed. Conclusions Combined SPE and GC–MS proved to be an efficient method to identify and quantify polar organic compounds in environmental samples. With respect to the concentrations measured in the present study, bisphenol A sometimes is the prominent compound in influent samples. Neither bisphenol A nor 4- tert -octylphenol or tech. 4-Nonylphenol show seasonal variations. However, there was a significant general trend of decreasing concentrations of tech. 4-Nonylphenol in influent and effluent samples from both WWTPs which probably reflects the implementing Directive 2003/53/EC. Recommendations and perspectives Further research is needed to investigate whether the observed decrease of tech. 4-Nonylphenol concentrations in German WWTPs since June 2004 will continue further on. The reason for the high effluent concentrations of bisphenol A in only a few samples has to be clarified in further research. The results from this study provide insight into the concentration development of the xenoestrogens bisphenol A, tech. 4-Nonylphenol, and 4- tert -octylphenol in WWTPs in the time span between 2003 and 2005.
Occurrence and temporal variations of the xenoestrogens bisphenol A, 4-tert-octylphenol, and tech. 4-Nonylphenol in two German wastewater treatment plantsEnvironmental science and pollution research international, 2008Co-Authors: Cornelia Höhne, Wilhelm PüttmannAbstract:
Goal, scope, and background
The xenoestrogens bisphenol A, 4-tert-octylphenol, and the technical isomer mixture of 4-Nonylphenol (tech. 4-Nonylphenol) belong to the group of chemicals which are called endocrine disrupters due to their property of causing hormonal dysfunctions in the endocrine system of organisms at very low concentrations. Bisphenol A, 4-tert-octylphenol, and the tech. 4-Nonylphenol (mixture of isomers) were determined in water samples collected from the influent and effluent of two German wastewater treatment plants (WWTP) during a long-time sampling period from February 2003 till August 2005 to assess their occurrence and temporal variations in WWTPs.
A.a. Mekkawy – One of the best experts on this subject based on the ideXlab platform.
“Toxic effects of 4-Nonylphenol on the embryonic development of African catfish Clarias gariepinus (Burchell, 1822) ”, 2012Co-Authors: Alaa El-din, Usama M. Mahmoud, H. Sayed, A.a. MekkawyAbstract:
The present study investigated effects of environmental relevant concentrations of 4-Nonylphenol (0.05 to 0.08 to 0.1 mg/l) on the reproduction and embryonic developmental stages of catfish (Clarias gariepinus). To determine the effect of 4-Nonylphenol on reproduction; catfish were exposed to three concentrations of 4-Nonylphenol in a flow-through-system during spawning period (some for one week and other for two weeks). At an estimated 4-Nonylphenol concentration the fertilization rate and hatching rate were significantly decreased with 4-Nonylphenol concentrations increasing while the incubation period, the mortality rate and malformed embryos ratio were increased. Also, the development of embryos and larvae was affected by 4-Nonylphenol in terms of morphological changes and histopathological alterations.
Reproductive biomarkers to identify endocrine disruption in Clarias gariepinus exposed to 4-Nonylphenol.Ecotoxicology and environmental safety, 2011Co-Authors: Alaa El-din H. Sayed, Usama M. Mahmoud, A.a. MekkawyAbstract:
The present study investigated the hormones concentrations and gonads alterations of Clarias gariepinus caused by sublethel concentrations of 4-Nonylphenol (0, 0.05, 0.08 and 0.1 mg/l). The changes in the activities of the hormones after exposure to these sublethel doses of 4-nonylpenol referred to endocrine disruption in Clarias gariepinus in association with histopathological changes in reproductive tissues. The levels of thyroid stimulating hormone (TSH), triiodothyronine (T3), total thyroxine (T4), follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone concentrations significantly decreased (P
Effects of 4-Nonylphenol on blood cells of the African catfish Clarias gariepinus (Burchell, 1822).Tissue & cell, 2011Co-Authors: A.a. Mekkawy, Usama M. Mahmoud, Alaa El-din H. SayedAbstract:
In the present work, the destructive effects of the 4-Nonylphenol on one of the most economically important Nile fishes, namely African catfish (Clarias gariepinus) were studied. Apoptosis, erythrocytes alterations, micronucleus test and blood parameters count were used as biological indicators to detect those effects. After exposure to sublethal concentrations of 4-Nonylphenol (0, 0.05, 0.08 and 0.1mg/l), apoptotic red blood cells with many malformations and micronucleated erythrocytes were recorded. Decrease in the blood parameters such as red blood cells (RBCs), hemoglobin (Hb), package cell volume (PCV), mean corpuscular hemoglobin concentration (MCHC), platelets, white blood cells (WBCs), lymphocytes, basophils, monocytes and increase in mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), neutrophils, eosinophils indicated the negative effects of 4-Nonylphenol. It was concluded that, the 4-Nonylphenol caused genotoxicity in erythrocytes with many malformations in shape and number indicated with other blood parameters.