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Alaa El-din H. Sayed - One of the best experts on this subject based on the ideXlab platform.
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γ h2ax foci as indication for the dna damage in erythrocytes of medaka oryzias latipes intoxicated with 4 Nonylphenol
Environmental Science and Pollution Research, 2020Co-Authors: Alaa El-din H. Sayed, Shoji Oda, Shosaku Kashiwada, Tomomi Watanabeasaka, Hiroshi MitaniAbstract:The present study aimed to investigate the genotoxicity in erythrocytes induced after exposure of medaka (Oryzias latipes) to 4-Nonylphenol (4-NP). Adult female medaka fish were exposed to 4-NP at three sublethal concentrations for 15 days to compare their sensitivity with that of catfish as an aquatic model. Comet assay and γ-H2AX were used as biomarkers to detect DNA damage in erythrocytes. Exposure to 4-NP resulted in an increase in the tail moment in a dose-dependent manner. The highest level of DNA damage was recorded after exposure to 100 μg/l 4-NP. The number of foci was increased after exposure to 4-NP, indicating damage to DNA. The present results confirmed the high level of morphological alterations and apoptosis of erythrocytes detected in the first part of this study. 4-NP induced genotoxic effects in medaka, which were found to be more sensitive than catfish after exposure to 4-Nonylphenol. Graphical abstract.
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sensitivity of medaka oryzias latipes to 4 Nonylphenol subacute exposure erythrocyte alterations and apoptosis
Environmental Toxicology and Pharmacology, 2018Co-Authors: Alaa El-din H. Sayed, Shoji Oda, Chisato Kataoka, Shosaku Kashiwada, Hiroshi MitaniAbstract:Abstract The present study was undertaken to assess the effects of the endocrine-disrupting compound; 4-Nonylphenol (4-NP) in medaka (Oryzias latipes). The frequencies of erythrocyte alterations, apoptosis, and micronuclei were used as biological indicators of damage. Medaka were exposed 15 days to 4-NP at three sublethal concentrations (50, 80, and 100 μg/l 4-NP) and results compared with those of a previous study using catfish as an animal model. Exposure of medaka resulted in a dose-dependent increase in the frequency of erythrocyte alterations, apoptosis and micronucleus (MN). Many morphological alterations and nuclear abnormalities were observed, including acanthocytes, lobed nucleus, eccentric nucleus, fragmented nucleus, blebbed nucleus, binuclei, deformed nucleus, notched nucleus, hemolysed cells, crenated cells, teardrop-like cells, and schistocytes. Mortality was recorded after treatment with 80 and 100 μg/l 4-NP, indicating that medaka are more sensitive than catfish to 4-NP exposure. We concluded that, 4-NP causes several malformations in the shape and number of erythrocytes in medaka, indicating its genotoxicity.
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modulatory effects of green tea extract against the hepatotoxic effects of 4 Nonylphenol in catfish clarias gariepinus
Ecotoxicology and Environmental Safety, 2018Co-Authors: Alaa El-din H. Sayed, Hamdy A M SolimanAbstract:The antioxidant role of the green tea (Camellia sinensis) extract (GTE) was examined to remedy the toxic effects of (0.2mgl-1) 4-Nonylphenol(4-NP). Biochemical parameters, antioxidant enzymes, liver lipid peroxidation (LPO), DNA fragmentation, and apoptosis as well as histopathology of liver of African catfish Clarias gariepinus were considered. Catfishes were divided into four groups: first group (control), second group (0.2mgl-1 of 4-NP), third group (0.2mgl-1of 4-NP +100mg GTE l-1water), and fourth group (0.2mgl-1 of 4-NP +200mg GTE l-1water). The results showed that significant increments of serum glucose, AST, ALT, total protein, total lipids, cholesterol, G6PDH, and cortisol. Meanwhile, serum acetylcholinesterase, ALP, and LDH were significantly reduced. In addition, antioxidant enzymes (SOD, CAT, GST, and TAC) levels were reduced in 4-NP treated fish compared to control. Also, there were significant increments in hepatic LPO, DNA fragmentation, and apoptotic erythrocytes in 4-NP treated fish compared to control. Liver of 4-NP treated fish showed some histopathological alterations such as, vacuolization in hepatocytes, congestion in central vein, infiltration of mononuclear inflammatory cells, and necrosis as well as depletion of glycogen content of liver. Addition of green tea extract into the water restored the alterations in most of those biomarkers induced by 4-NP. We concluded that, GTE has a protective role against hepatic failure, depletion of antioxidant defense, and genotoxicity induced 4-NP in C. gariepinus.
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Protective effect of Nigella sativa on 4-Nonylphenol-induced nephrotoxicity in Clarias gariepinus (Burchell, 1822)
The Science of the total environment, 2017Co-Authors: Ahmed Kotb, Mahmoud Abd-elkareem, Nasser S. Abou Khalil, Alaa El-din H. SayedAbstract:Abstract The aim of this study was to examine the protective effects of Nigella sativa ( N. sativa ) on 4-Nonylphenol-induced nephrotoxicity in Clarias gariepinus . 30 fishes were divided into five groups: control, 4-Nonylphenol-treated, 1% N. sativa treated, 2.5% N. sativa treated, and 5% N. sativa treated. N. sativa and 4-Nonylphenol were given for 3 weeks. 4-NP and 4-NP- N. sativa treated fishes were compared with the control group. Kidney histology, immunochemistry, and electron microscope were assessed after 4-NP exposure. In the African catfish, 4-NP is mainly excreted through the kidney causing nephrotoxicity. Our results showed that 4-NP administration significantly disturbed the kidney structure and function. 4-NP treated fishes showed dilated glomerular vessels, fewer glomerular cells content, decreased expressions of glomerular proteins, and increased level of autophagy compared to control group ( P N. sativa has different immunological and pharmacological effects such as anti-apoptotic and anti-oxidant, therefore, the administration of N. sativa with 4-Nonylphenol significantly minimize the nephrotoxic effect of 4-NP and maintain the normal kidney structure and function. Our novel study demonstrated for the first time that N. sativa could protect the kidney against 4-NP induced-nephrotoxicity.
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induction of apoptosis and dna damage by 4 Nonylphenol in african catfish clarias gariepinus and the antioxidant role of cydonia oblonga
Ecotoxicology and Environmental Safety, 2017Co-Authors: Alaa El-din H. Sayed, Heba S HamedAbstract:In this study, we assessed the toxic effects of sub lethal concentration (0.1mgl-1) 4-Nonylphenol (4-NP) on serum biochemical parameters, liver lipid peroxidation (LPO) and antioxidant enzymes of the African catfish Clarias gariepinus for 14 days and the ability of the quince leaf extract to alleviate the effects of (4-NP). Fish were categorized into four groups: control, exposure to 0.1mgl-1 4-NP, exposure to 0.1mgl-1 4-NP with quince leaf extract (10ml/30L water), and exposure to 0.1mgl-1 4-NP with quince leaf extract (20ml/30L water). 4-NP exposure induced a significant (p<0.05) increase in the levels of glucose, AST, ALT, creatinine, urea, uric acid, cholesterol, and G6PDH as well as, the percentages of hepatic LPO level, DNA fragmentation, and apoptotic erythrocytes (p<0.05). A significant (p<0.05) decrease in alkaline phosphatase (ALP), total protein, albumin, globulin, total lipids, and LDH were also recorded. Liver enzyme activities (SOD, CAT and TAC) were increased. Addition of the quince leaf extract into the water was able to reinstate the alterations in biochemical parameters, antioxidant biomarkers, apoptotic level and hepatic DNA damage induced by 4-NP.
Qunfang Zhou - One of the best experts on this subject based on the ideXlab platform.
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determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in surface waters from the haihe river in tianjin by gas chromatography mass spectrometry with selected ion monitoring
Chemosphere, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Jingfu Liu, Qunfang ZhouAbstract:The estrogenic pollutants 4-tert-octylphenol (OP), 4-Nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted and concentrated from 300 ml acidified water samples by liquid-liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 mugl(-1)) and NP (0.55 mugl(-1)). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0-20.2, 106-296 and 19.1-106 ngl(-1), respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%. (C) 2004 Elsevier Ltd. All rights reserved.
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determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in surface waters from the haihe river in tianjin by gas chromatography mass spectrometry with selected ion monitoring
Chemosphere, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Jingfu Liu, Qunfang ZhouAbstract:The estrogenic pollutants 4-tert-octylphenol (OP), 4-Nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted and concentrated from 300 ml acidified water samples by liquid-liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 microgl(-1)) and NP (0.55 microgl(-1)). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0-20.2, 106-296 and 19.1-106 ngl(-1), respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%.
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simultaneous determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in guanting reservoir using gas chromatography mass spectrometry with selected ion monitoring
Journal of Environmental Sciences-china, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Qunfang Zhou, Jingfu LiuAbstract:The wide occurrence of estrogenic compounds 4-tert-octylphenol, 4-Nonylphenol, bisphenol A in surface water of Guanting Reservoir was successfully determined. The target compounds in water samples were preconcentrated by liquid-liquid extraction with dichloromethane, derivatized by trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). In the selected seven sampling sites (S1-S7), the concentration of NP in sample S7 was significantly higher than the other in reservoir. The status of pollution in S3 and S7 were much more serious. The concentrations of OP, NP and BPA were in the range of 44.5-48.8, 221.6-349.6 and 30.2-82.7 ng/L, respectively. The pollutants were mainly inputted from the upper river and released from sediments in Guanting Reservoir.
Jingfu Liu - One of the best experts on this subject based on the ideXlab platform.
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determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in surface waters from the haihe river in tianjin by gas chromatography mass spectrometry with selected ion monitoring
Chemosphere, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Jingfu Liu, Qunfang ZhouAbstract:The estrogenic pollutants 4-tert-octylphenol (OP), 4-Nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted and concentrated from 300 ml acidified water samples by liquid-liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 mugl(-1)) and NP (0.55 mugl(-1)). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0-20.2, 106-296 and 19.1-106 ngl(-1), respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%. (C) 2004 Elsevier Ltd. All rights reserved.
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determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in surface waters from the haihe river in tianjin by gas chromatography mass spectrometry with selected ion monitoring
Chemosphere, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Jingfu Liu, Qunfang ZhouAbstract:The estrogenic pollutants 4-tert-octylphenol (OP), 4-Nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted and concentrated from 300 ml acidified water samples by liquid-liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 microgl(-1)) and NP (0.55 microgl(-1)). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0-20.2, 106-296 and 19.1-106 ngl(-1), respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%.
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simultaneous determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in guanting reservoir using gas chromatography mass spectrometry with selected ion monitoring
Journal of Environmental Sciences-china, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Qunfang Zhou, Jingfu LiuAbstract:The wide occurrence of estrogenic compounds 4-tert-octylphenol, 4-Nonylphenol, bisphenol A in surface water of Guanting Reservoir was successfully determined. The target compounds in water samples were preconcentrated by liquid-liquid extraction with dichloromethane, derivatized by trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). In the selected seven sampling sites (S1-S7), the concentration of NP in sample S7 was significantly higher than the other in reservoir. The status of pollution in S3 and S7 were much more serious. The concentrations of OP, NP and BPA were in the range of 44.5-48.8, 221.6-349.6 and 30.2-82.7 ng/L, respectively. The pollutants were mainly inputted from the upper river and released from sediments in Guanting Reservoir.
Xinglong Jin - One of the best experts on this subject based on the ideXlab platform.
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determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in surface waters from the haihe river in tianjin by gas chromatography mass spectrometry with selected ion monitoring
Chemosphere, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Jingfu Liu, Qunfang ZhouAbstract:The estrogenic pollutants 4-tert-octylphenol (OP), 4-Nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted and concentrated from 300 ml acidified water samples by liquid-liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 mugl(-1)) and NP (0.55 mugl(-1)). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0-20.2, 106-296 and 19.1-106 ngl(-1), respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%. (C) 2004 Elsevier Ltd. All rights reserved.
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determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in surface waters from the haihe river in tianjin by gas chromatography mass spectrometry with selected ion monitoring
Chemosphere, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Jingfu Liu, Qunfang ZhouAbstract:The estrogenic pollutants 4-tert-octylphenol (OP), 4-Nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted and concentrated from 300 ml acidified water samples by liquid-liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 microgl(-1)) and NP (0.55 microgl(-1)). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0-20.2, 106-296 and 19.1-106 ngl(-1), respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%.
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simultaneous determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in guanting reservoir using gas chromatography mass spectrometry with selected ion monitoring
Journal of Environmental Sciences-china, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Qunfang Zhou, Jingfu LiuAbstract:The wide occurrence of estrogenic compounds 4-tert-octylphenol, 4-Nonylphenol, bisphenol A in surface water of Guanting Reservoir was successfully determined. The target compounds in water samples were preconcentrated by liquid-liquid extraction with dichloromethane, derivatized by trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). In the selected seven sampling sites (S1-S7), the concentration of NP in sample S7 was significantly higher than the other in reservoir. The status of pollution in S3 and S7 were much more serious. The concentrations of OP, NP and BPA were in the range of 44.5-48.8, 221.6-349.6 and 30.2-82.7 ng/L, respectively. The pollutants were mainly inputted from the upper river and released from sediments in Guanting Reservoir.
Guolan Huang - One of the best experts on this subject based on the ideXlab platform.
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determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in surface waters from the haihe river in tianjin by gas chromatography mass spectrometry with selected ion monitoring
Chemosphere, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Jingfu Liu, Qunfang ZhouAbstract:The estrogenic pollutants 4-tert-octylphenol (OP), 4-Nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted and concentrated from 300 ml acidified water samples by liquid-liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 mugl(-1)) and NP (0.55 mugl(-1)). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0-20.2, 106-296 and 19.1-106 ngl(-1), respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%. (C) 2004 Elsevier Ltd. All rights reserved.
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determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in surface waters from the haihe river in tianjin by gas chromatography mass spectrometry with selected ion monitoring
Chemosphere, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Jingfu Liu, Qunfang ZhouAbstract:The estrogenic pollutants 4-tert-octylphenol (OP), 4-Nonylphenol (NP) and bisphenol A (BPA) were determined in surface water samples from the Haihe River, Tianjin, China. The analytes were extracted and concentrated from 300 ml acidified water samples by liquid-liquid extractions using dichloromethane, derivatized with trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). Among the samples collected from 14 sampling sites, only one sample was found to have a relatively high concentration of BPA (8.30 microgl(-1)) and NP (0.55 microgl(-1)). The concentrations of OP, NP and BPA in the other samples were in the range of 18.0-20.2, 106-296 and 19.1-106 ngl(-1), respectively. Recoveries for OP, NP and BPA in the spiked water samples were all over 80%.
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simultaneous determination of 4 tert octylphenol 4 Nonylphenol and bisphenol a in guanting reservoir using gas chromatography mass spectrometry with selected ion monitoring
Journal of Environmental Sciences-china, 2004Co-Authors: Xinglong Jin, Guolan Huang, Guibin Jiang, Qunfang Zhou, Jingfu LiuAbstract:The wide occurrence of estrogenic compounds 4-tert-octylphenol, 4-Nonylphenol, bisphenol A in surface water of Guanting Reservoir was successfully determined. The target compounds in water samples were preconcentrated by liquid-liquid extraction with dichloromethane, derivatized by trifluoroacetic anhydride, and quantified by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring (SIM). In the selected seven sampling sites (S1-S7), the concentration of NP in sample S7 was significantly higher than the other in reservoir. The status of pollution in S3 and S7 were much more serious. The concentrations of OP, NP and BPA were in the range of 44.5-48.8, 221.6-349.6 and 30.2-82.7 ng/L, respectively. The pollutants were mainly inputted from the upper river and released from sediments in Guanting Reservoir.
