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5-Methylcytidine

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Yinsheng Wang – One of the best experts on this subject based on the ideXlab platform.

  • simultaneous quantification of methylated cytidine and adenosine in cellular and tissue rna by nano flow liquid chromatography tandem mass spectrometry coupled with the stable isotope dilution method
    Analytical Chemistry, 2015
    Co-Authors: Nicholas J Amato, Pengcheng Wang, Sara J Mcgowan, Laura J Niedernhofer, Yinsheng Wang
    Abstract:

    The rising interest in understanding the functions, regulation, and maintenance of the epitranscriptome calls for robust and accurate analytical methods for the identification and quantification of post-transcriptionally modified nucleosides in RNA. Monomethylations of cytidine and adenosine are common post-transcriptional modifications in RNA. Herein, we developed an LC–MS/MS/MS coupled with the stable isotope-dilution method for the sensitive and accurate quantifications of 5-Methylcytidine (m5C), 2′-O-methylcytidine (Cm), N6-methyladenosine (m6A), and 2′-O-methyladenosine (Am) in RNA isolated from mammalian cells and tissues. Our results showed that the distributions of m5C, Cm and Am are tissue-specific. In addition, the 2′-O-methylated ribonucleosides (Cm and Am) are present at higher levels than the corresponding methylated nucleobase products (m5C and m6A) in total RNA isolated from mouse brain, pancreas, and spleen but not mouse heart. We also found that the levels of m5C, Cm, and Am are significa…

  • Simultaneous Quantification of Methylated Cytidine and Adenosine in Cellular and Tissue RNA by Nano-Flow Liquid Chromatography–Tandem Mass Spectrometry Coupled with the Stable Isotope-Dilution Method
    Analytical chemistry, 2015
    Co-Authors: Nicholas J Amato, Pengcheng Wang, Sara J Mcgowan, Laura J Niedernhofer, Yinsheng Wang
    Abstract:

    The rising interest in understanding the functions, regulation, and maintenance of the epitranscriptome calls for robust and accurate analytical methods for the identification and quantification of post-transcriptionally modified nucleosides in RNA. Monomethylations of cytidine and adenosine are common post-transcriptional modifications in RNA. Herein, we developed an LC–MS/MS/MS coupled with the stable isotope-dilution method for the sensitive and accurate quantifications of 5-Methylcytidine (m5C), 2′-O-methylcytidine (Cm), N6-methyladenosine (m6A), and 2′-O-methyladenosine (Am) in RNA isolated from mammalian cells and tissues. Our results showed that the distributions of m5C, Cm and Am are tissue-specific. In addition, the 2′-O-methylated ribonucleosides (Cm and Am) are present at higher levels than the corresponding methylated nucleobase products (m5C and m6A) in total RNA isolated from mouse brain, pancreas, and spleen but not mouse heart. We also found that the levels of m5C, Cm, and Am are significa…

Nicholas J Amato – One of the best experts on this subject based on the ideXlab platform.

  • simultaneous quantification of methylated cytidine and adenosine in cellular and tissue rna by nano flow liquid chromatography tandem mass spectrometry coupled with the stable isotope dilution method
    Analytical Chemistry, 2015
    Co-Authors: Nicholas J Amato, Pengcheng Wang, Sara J Mcgowan, Laura J Niedernhofer, Yinsheng Wang
    Abstract:

    The rising interest in understanding the functions, regulation, and maintenance of the epitranscriptome calls for robust and accurate analytical methods for the identification and quantification of post-transcriptionally modified nucleosides in RNA. Monomethylations of cytidine and adenosine are common post-transcriptional modifications in RNA. Herein, we developed an LC–MS/MS/MS coupled with the stable isotope-dilution method for the sensitive and accurate quantifications of 5-Methylcytidine (m5C), 2′-O-methylcytidine (Cm), N6-methyladenosine (m6A), and 2′-O-methyladenosine (Am) in RNA isolated from mammalian cells and tissues. Our results showed that the distributions of m5C, Cm and Am are tissue-specific. In addition, the 2′-O-methylated ribonucleosides (Cm and Am) are present at higher levels than the corresponding methylated nucleobase products (m5C and m6A) in total RNA isolated from mouse brain, pancreas, and spleen but not mouse heart. We also found that the levels of m5C, Cm, and Am are significa…

  • Simultaneous Quantification of Methylated Cytidine and Adenosine in Cellular and Tissue RNA by Nano-Flow Liquid Chromatography–Tandem Mass Spectrometry Coupled with the Stable Isotope-Dilution Method
    Analytical chemistry, 2015
    Co-Authors: Nicholas J Amato, Pengcheng Wang, Sara J Mcgowan, Laura J Niedernhofer, Yinsheng Wang
    Abstract:

    The rising interest in understanding the functions, regulation, and maintenance of the epitranscriptome calls for robust and accurate analytical methods for the identification and quantification of post-transcriptionally modified nucleosides in RNA. Monomethylations of cytidine and adenosine are common post-transcriptional modifications in RNA. Herein, we developed an LC–MS/MS/MS coupled with the stable isotope-dilution method for the sensitive and accurate quantifications of 5-Methylcytidine (m5C), 2′-O-methylcytidine (Cm), N6-methyladenosine (m6A), and 2′-O-methyladenosine (Am) in RNA isolated from mammalian cells and tissues. Our results showed that the distributions of m5C, Cm and Am are tissue-specific. In addition, the 2′-O-methylated ribonucleosides (Cm and Am) are present at higher levels than the corresponding methylated nucleobase products (m5C and m6A) in total RNA isolated from mouse brain, pancreas, and spleen but not mouse heart. We also found that the levels of m5C, Cm, and Am are significa…

  • Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
    Journal of the American Chemical Society, 2014
    Co-Authors: Candace R. Guerrero, Nicholas J Amato, Na Zhong, Yunhua Liu, Shuo Liu, Qian Cai, Seung Gi Jin, Laura J Niedernhofer
    Abstract:

    Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-Methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

Laura J Niedernhofer – One of the best experts on this subject based on the ideXlab platform.

  • simultaneous quantification of methylated cytidine and adenosine in cellular and tissue rna by nano flow liquid chromatography tandem mass spectrometry coupled with the stable isotope dilution method
    Analytical Chemistry, 2015
    Co-Authors: Nicholas J Amato, Pengcheng Wang, Sara J Mcgowan, Laura J Niedernhofer, Yinsheng Wang
    Abstract:

    The rising interest in understanding the functions, regulation, and maintenance of the epitranscriptome calls for robust and accurate analytical methods for the identification and quantification of post-transcriptionally modified nucleosides in RNA. Monomethylations of cytidine and adenosine are common post-transcriptional modifications in RNA. Herein, we developed an LC–MS/MS/MS coupled with the stable isotope-dilution method for the sensitive and accurate quantifications of 5-Methylcytidine (m5C), 2′-O-methylcytidine (Cm), N6-methyladenosine (m6A), and 2′-O-methyladenosine (Am) in RNA isolated from mammalian cells and tissues. Our results showed that the distributions of m5C, Cm and Am are tissue-specific. In addition, the 2′-O-methylated ribonucleosides (Cm and Am) are present at higher levels than the corresponding methylated nucleobase products (m5C and m6A) in total RNA isolated from mouse brain, pancreas, and spleen but not mouse heart. We also found that the levels of m5C, Cm, and Am are significa…

  • Simultaneous Quantification of Methylated Cytidine and Adenosine in Cellular and Tissue RNA by Nano-Flow Liquid Chromatography–Tandem Mass Spectrometry Coupled with the Stable Isotope-Dilution Method
    Analytical chemistry, 2015
    Co-Authors: Nicholas J Amato, Pengcheng Wang, Sara J Mcgowan, Laura J Niedernhofer, Yinsheng Wang
    Abstract:

    The rising interest in understanding the functions, regulation, and maintenance of the epitranscriptome calls for robust and accurate analytical methods for the identification and quantification of post-transcriptionally modified nucleosides in RNA. Monomethylations of cytidine and adenosine are common post-transcriptional modifications in RNA. Herein, we developed an LC–MS/MS/MS coupled with the stable isotope-dilution method for the sensitive and accurate quantifications of 5-Methylcytidine (m5C), 2′-O-methylcytidine (Cm), N6-methyladenosine (m6A), and 2′-O-methyladenosine (Am) in RNA isolated from mammalian cells and tissues. Our results showed that the distributions of m5C, Cm and Am are tissue-specific. In addition, the 2′-O-methylated ribonucleosides (Cm and Am) are present at higher levels than the corresponding methylated nucleobase products (m5C and m6A) in total RNA isolated from mouse brain, pancreas, and spleen but not mouse heart. We also found that the levels of m5C, Cm, and Am are significa…

  • Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
    Journal of the American Chemical Society, 2014
    Co-Authors: Candace R. Guerrero, Nicholas J Amato, Na Zhong, Yunhua Liu, Shuo Liu, Qian Cai, Seung Gi Jin, Laura J Niedernhofer
    Abstract:

    Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-Methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.