5S Ribosomal RNA

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 168 Experts worldwide ranked by ideXlab platform

David A. Campbell - One of the best experts on this subject based on the ideXlab platform.

  • 5S Ribosomal RNA gene repeat sequences define at least eight groups of plant trypanosomatids (Phytomonas spp.): phloem-restricted pathogens form a distinct section.
    Journal of Eukaryotic Microbiology, 2000
    Co-Authors: Michel Dollet, Nancy R. Sturm, Manuel Sánchez-moreno, David A. Campbell
    Abstract:

    Trypanosomatids isolated from plants have been assigned typically into the genus Phytomonas. Such designations do not reflect the biology of the diverse isolates; confusion may arise due to the transient presence in plants of monogenetic (insect) trypanosomatids deposited by phytophagous bugs. To develop further molecular markers for the plant kinetoplastids, we have obtained the DNA sequence of the 5S Ribosomal RNA gene from 24 isolates harvested from phloem, latex, and fruit. Small, distinct sequence differences were found at the 3'-ends of the transcribed regions; substantial sequence and size differences were found in the non-transcribed regions. Alignment of the gene sequences from all the isolates suggested the presence of eight groupings. While six groups contained isolates from single plant tissues, groups C and A contained isolates from both fruit and latex. The DNA sequences of the 10 phloem-restricted pathogenic isolates from South America and the Carribean were highly conserved and thus comprised a single group (H). The conserved nature of the 5S Ribosomal RNA genes in these plant pathogens supports the proposal that they be considered as a distinct section, the phloemicola.

  • 5S Ribosomal RNA gene repeat sequences define at least eight groups of plant trypanosomatids phytomonas spp phloem restricted pathogens form a distinct section
    Journal of Eukaryotic Microbiology, 2000
    Co-Authors: Michel Dollet, Nancy R. Sturm, Manuel Sanchezmoreno, David A. Campbell
    Abstract:

    Abstract Trypanosomatids isolated from plants have been assigned typically into the genus Phytomonas. Such designations do not reflect the biology of the diverse isolates; confusion may arise due to the transient presence in plants of monogenetic (insect) trypanosomatids deposited by phytophagous bugs. To develop further molecular markers for the plant kinetoplastids, we have obtained the DNA sequence of the 5S Ribosomal RNA gene from 24 isolates harvested from phloem, latex, and fruit. Small, distinct sequence differences were found at the 3′-ends of the transcribed regions; substantial sequence and size differences were found in the non-transcribed regions. Alignment of the gene sequences from all the isolates suggested the presence of eight groupings. While six groups contained isolates from single plant tissues, groups C and A contained isolates from both fruit and latex. The DNA sequences of the 10 phloem-restricted pathogenic isolates from South America and the Carribean were highly conserved and th...

Michel Dollet - One of the best experts on this subject based on the ideXlab platform.

  • 5S Ribosomal RNA gene repeat sequences define at least eight groups of plant trypanosomatids (Phytomonas spp.): phloem-restricted pathogens form a distinct section.
    Journal of Eukaryotic Microbiology, 2000
    Co-Authors: Michel Dollet, Nancy R. Sturm, Manuel Sánchez-moreno, David A. Campbell
    Abstract:

    Trypanosomatids isolated from plants have been assigned typically into the genus Phytomonas. Such designations do not reflect the biology of the diverse isolates; confusion may arise due to the transient presence in plants of monogenetic (insect) trypanosomatids deposited by phytophagous bugs. To develop further molecular markers for the plant kinetoplastids, we have obtained the DNA sequence of the 5S Ribosomal RNA gene from 24 isolates harvested from phloem, latex, and fruit. Small, distinct sequence differences were found at the 3'-ends of the transcribed regions; substantial sequence and size differences were found in the non-transcribed regions. Alignment of the gene sequences from all the isolates suggested the presence of eight groupings. While six groups contained isolates from single plant tissues, groups C and A contained isolates from both fruit and latex. The DNA sequences of the 10 phloem-restricted pathogenic isolates from South America and the Carribean were highly conserved and thus comprised a single group (H). The conserved nature of the 5S Ribosomal RNA genes in these plant pathogens supports the proposal that they be considered as a distinct section, the phloemicola.

  • 5S Ribosomal RNA gene repeat sequences define at least eight groups of plant trypanosomatids phytomonas spp phloem restricted pathogens form a distinct section
    Journal of Eukaryotic Microbiology, 2000
    Co-Authors: Michel Dollet, Nancy R. Sturm, Manuel Sanchezmoreno, David A. Campbell
    Abstract:

    Abstract Trypanosomatids isolated from plants have been assigned typically into the genus Phytomonas. Such designations do not reflect the biology of the diverse isolates; confusion may arise due to the transient presence in plants of monogenetic (insect) trypanosomatids deposited by phytophagous bugs. To develop further molecular markers for the plant kinetoplastids, we have obtained the DNA sequence of the 5S Ribosomal RNA gene from 24 isolates harvested from phloem, latex, and fruit. Small, distinct sequence differences were found at the 3′-ends of the transcribed regions; substantial sequence and size differences were found in the non-transcribed regions. Alignment of the gene sequences from all the isolates suggested the presence of eight groupings. While six groups contained isolates from single plant tissues, groups C and A contained isolates from both fruit and latex. The DNA sequences of the 10 phloem-restricted pathogenic isolates from South America and the Carribean were highly conserved and th...

Nancy R. Sturm - One of the best experts on this subject based on the ideXlab platform.

  • 5S Ribosomal RNA gene repeat sequences define at least eight groups of plant trypanosomatids (Phytomonas spp.): phloem-restricted pathogens form a distinct section.
    Journal of Eukaryotic Microbiology, 2000
    Co-Authors: Michel Dollet, Nancy R. Sturm, Manuel Sánchez-moreno, David A. Campbell
    Abstract:

    Trypanosomatids isolated from plants have been assigned typically into the genus Phytomonas. Such designations do not reflect the biology of the diverse isolates; confusion may arise due to the transient presence in plants of monogenetic (insect) trypanosomatids deposited by phytophagous bugs. To develop further molecular markers for the plant kinetoplastids, we have obtained the DNA sequence of the 5S Ribosomal RNA gene from 24 isolates harvested from phloem, latex, and fruit. Small, distinct sequence differences were found at the 3'-ends of the transcribed regions; substantial sequence and size differences were found in the non-transcribed regions. Alignment of the gene sequences from all the isolates suggested the presence of eight groupings. While six groups contained isolates from single plant tissues, groups C and A contained isolates from both fruit and latex. The DNA sequences of the 10 phloem-restricted pathogenic isolates from South America and the Carribean were highly conserved and thus comprised a single group (H). The conserved nature of the 5S Ribosomal RNA genes in these plant pathogens supports the proposal that they be considered as a distinct section, the phloemicola.

  • 5S Ribosomal RNA gene repeat sequences define at least eight groups of plant trypanosomatids phytomonas spp phloem restricted pathogens form a distinct section
    Journal of Eukaryotic Microbiology, 2000
    Co-Authors: Michel Dollet, Nancy R. Sturm, Manuel Sanchezmoreno, David A. Campbell
    Abstract:

    Abstract Trypanosomatids isolated from plants have been assigned typically into the genus Phytomonas. Such designations do not reflect the biology of the diverse isolates; confusion may arise due to the transient presence in plants of monogenetic (insect) trypanosomatids deposited by phytophagous bugs. To develop further molecular markers for the plant kinetoplastids, we have obtained the DNA sequence of the 5S Ribosomal RNA gene from 24 isolates harvested from phloem, latex, and fruit. Small, distinct sequence differences were found at the 3′-ends of the transcribed regions; substantial sequence and size differences were found in the non-transcribed regions. Alignment of the gene sequences from all the isolates suggested the presence of eight groupings. While six groups contained isolates from single plant tissues, groups C and A contained isolates from both fruit and latex. The DNA sequences of the 10 phloem-restricted pathogenic isolates from South America and the Carribean were highly conserved and th...

Manuel Sanchezmoreno - One of the best experts on this subject based on the ideXlab platform.

  • 5S Ribosomal RNA gene repeat sequences define at least eight groups of plant trypanosomatids phytomonas spp phloem restricted pathogens form a distinct section
    Journal of Eukaryotic Microbiology, 2000
    Co-Authors: Michel Dollet, Nancy R. Sturm, Manuel Sanchezmoreno, David A. Campbell
    Abstract:

    Abstract Trypanosomatids isolated from plants have been assigned typically into the genus Phytomonas. Such designations do not reflect the biology of the diverse isolates; confusion may arise due to the transient presence in plants of monogenetic (insect) trypanosomatids deposited by phytophagous bugs. To develop further molecular markers for the plant kinetoplastids, we have obtained the DNA sequence of the 5S Ribosomal RNA gene from 24 isolates harvested from phloem, latex, and fruit. Small, distinct sequence differences were found at the 3′-ends of the transcribed regions; substantial sequence and size differences were found in the non-transcribed regions. Alignment of the gene sequences from all the isolates suggested the presence of eight groupings. While six groups contained isolates from single plant tissues, groups C and A contained isolates from both fruit and latex. The DNA sequences of the 10 phloem-restricted pathogenic isolates from South America and the Carribean were highly conserved and th...

Jan Barciszewski - One of the best experts on this subject based on the ideXlab platform.

  • 5S Ribosomal RNA database
    Nucleic Acids Research, 2002
    Co-Authors: Maciej Szymanski, Miroslawa Z Barciszewska, Volker A Erdmann, Jan Barciszewski
    Abstract:

    Ribosomal 5S RNA (5S rRNA) is an integral component of the large Ribosomal subunit in all known organisms with the exception only of mitochondrial ribosomes of fungi and animals. It is thought to enhance protein synthesis by stabilization of a ribosome structure. This paper presents the updated database of 5S rRNA and their genes (5S rDNA). Its short characteristics are presented in the Introduction. The database contains 2280 primary structures of 5S rRNA and 5S rRNA genes. These include 536 eubacterial, 61 archaebacterial, 1611 eukaryotic and 72 organelle sequences. The database is available on line through the World Wide Web at http://biobases.ibch.poznan.pl/5SData/.

  • 5S Ribosomal RNA
    Biomacromolecules, 2000
    Co-Authors: Miroslawa Z Barciszewska, Maciej Szymanski, Volker A Erdmann, Jan Barciszewski
    Abstract:

    : Ribosomes have been visualized in electron micrographs in 1943 but 5S rRNA was discovered 20 years later. The next four decades witnessed big advances in our understanding of the ribosome using biochemical, genetic and low resolution structural approaches. During those times many experimental data accumulates also on 5S rRNA, but its precise function remains unknown. To understand the role of this RNA in ribosome a high-resolution structure is urgently needed. Because the ribosome is a dynamic machine, details on the interaction of 5S rRNA with proteins within entire ribosome are required. Big progress in the structural analysis of ribosome will stimulate further understanding of 5S rRNA.

  • 5S Ribosomal RNA database y2k
    Nucleic Acids Research, 2000
    Co-Authors: Maciej Szymanski, Miroslawa Z Barciszewska, Jan Barciszewski, Volker A Erdmann
    Abstract:

    This paper presents the updated version (Y2K) of the database of Ribosomal 5S ribonucleic acids (5S rRNA) and their genes (5S rDNA), http://rose.man/poznan. pl/5SData/index.html . This edition of the database contains 1985 primary structures of 5S rRNA and 5S rDNA. They include 60 archaebacterial, 470 eubacterial, 63 plastid, nine mitochondrial and 1383 eukaryotic sequences. The nucleotide sequences of the 5S rRNAs or 5S rDNAs are divided according to the taxonomic position of the source organisms.

  • 5S Ribosomal RNA data bank
    Nucleic Acids Research, 1999
    Co-Authors: Maciej Szymanski, Miroslawa Z Barciszewska, Jan Barciszewski, Volker A Erdmann
    Abstract:

    This paper presents the updated version of the data base of Ribosomal 5S ribonucleic acids (5S rRNA) and their genes (5S rDNA). This edition of the data bank contains 1889 primary structures of 5S rRNA and 5S rDNA. These include 60 archaebacterial, 439 eubacterial, 63 plastid, 9 mitochondrial and 1318 eukaryotic sequences. The nucleotide sequences of 5S rRNAs or 5S rDNAs are divided according to the taxonomic position of organisms. The sequences stored in the database can be viewed and retrieved using the taxonomic browser at the URL: http://rose.man.poznan.pl/5SData/5SRNA.html++ +

  • The nucleotide sequence of 5S Ribosomal RNA from the parasitic nematode Ascaris suum. Evolutionary relationships in nematodes.
    Biochimica et Biophysica Acta, 1996
    Co-Authors: Maciej Szymański, Miroslawa Z Barciszewska, Tomasz Opioła, Jan Barciszewski
    Abstract:

    We have determined the nucleotide sequence of 5S Ribosomal RNA from the parasitic nematode Ascaris suum. The analysis of all Nematoda 5S rRNAs and their genes shows that this group must have diverged from other Metazoa at early stages of evolution. This conclusion is supported by the sequence variability in single-stranded regions which are strongly conserved in animal 5S rRNAs.